(Circular) (Six-base) MAPSORT of: pFN21AA0564.seq Check: 1090 from: 1 to: 10420 pFN21AA0564 10420 bp With 182 enzymes: SgfI * September 16, 2010 15:14 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2077 4401 2077 Size: 2324 8096 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8230 278 Size: 53 83 186 936 6694 2468 Fragments arranged by size: 6694 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6434 6449 7763 1937 Size: 4497 15 1314 4594 Fragments arranged by size: 4594 4497 1314 15 Acc65I G'GTAC_C Cuts at: 4754 7741 4754 Size: 2987 7433 AclI AA'CG_TT Cuts at: 1563 5141 8547 8920 1563 Size: 3578 3406 373 3063 Fragments arranged by size: 3578 3406 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3715 4181 4442 4844 5001 5319 7391 7601 Size: 2083 466 261 402 157 318 2072 210 Cuts at: 7601 8483 9867 10135 1632 Size: 882 1384 268 1917 Fragments arranged by size: 2083 2072 1917 1384 882 466 402 318 268 261 210 157 AfeI AGC'GCT Cuts at: 3086 3086 Size: 10420 AflII C'TTAA_G Cuts at: 820 1017 2828 820 Size: 197 1811 8412 Fragments arranged by size: 8412 1811 197 AflIII A'CryG_T Cuts at: 1203 4574 7416 9319 10296 1203 Size: 3371 2842 1903 977 1327 Fragments arranged by size: 3371 2842 1903 1327 977 AgeI A'CCGG_T Cuts at: 9251 9251 Size: 10420 AhdI GACnn_n'nnGTC Cuts at: 4983 5163 9149 4983 Size: 180 3986 6254 Fragments arranged by size: 6254 3986 180 AleI CACnn'nnGTG Cuts at: 1166 3781 1166 Size: 2615 7805 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 1184 Size: 906 9514 AlwNI CAG_nnn'CTG Cuts at: 3787 6941 8163 9735 3787 Size: 3154 1222 1572 4472 Fragments arranged by size: 4472 3154 1572 1222 ApaI G_GGCC'C Cuts at: 5608 5608 Size: 10420 ApaLI G'TGCA_C Cuts at: 4215 6956 8477 9633 4215 Size: 2741 1521 1156 5002 Fragments arranged by size: 5002 2741 1521 1156 ApoI r'AATT_y Cuts at: 1493 2208 2707 3797 3996 4642 4970 5702 6006 Size: 715 499 1090 199 646 328 732 304 Cuts at: 6006 6078 7365 7729 7955 8107 10216 1493 Size: 72 1287 364 226 152 2109 1697 Fragments arranged by size: 2109 1697 1287 1090 732 715 646 499 364 328 304 226 199 152 72 AseI AT'TA_AT Cuts at: 160 1032 5337 8974 160 Size: 872 4305 3637 1606 Fragments arranged by size: 4305 3637 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10420 AvaI C'yCGr_G Cuts at: 1870 1943 2027 2152 4407 4814 7745 1870 Size: 73 84 125 2255 407 2931 4545 Fragments arranged by size: 4545 2931 2255 407 125 84 73 AvrII C'CTAG_G Cuts at: 4735 4735 Size: 10420 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4425 4458 5240 5273 4425 Size: 33 782 33 9572 Fragments arranged by size: 9572 782 33 33 BamHI G'GATC_C Cuts at: 3943 7750 8117 3943 Size: 3807 367 6246 Fragments arranged by size: 6246 3807 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 3217 4754 5499 5518 Size: 325 217 627 245 1185 1537 745 19 Cuts at: 5518 7741 9196 618 Size: 2223 1455 1842 Fragments arranged by size: 2223 1842 1537 1455 1185 745 627 325 245 217 19 BanII G_rGCy'C Cuts at: 1391 4815 4854 5608 7739 1391 Size: 3424 39 754 2131 4072 Fragments arranged by size: 4072 3424 2131 754 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3651 4500 4633 7379 928 Size: 971 1752 849 133 2746 3969 Fragments arranged by size: 3969 2746 1752 971 849 133 BbvCI CC'TCA_GC Cuts at: 4889 4889 Size: 10420 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2387 2421 8611 8645 1903 Size: 34 50 34 366 34 6190 34 3678 Fragments arranged by size: 6190 3678 366 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2203 3392 4029 4426 5070 5268 7651 8312 9522 Size: 1189 637 397 644 198 2383 661 1210 Cuts at: 9522 2203 Size: 3101 Fragments arranged by size: 3101 2383 1210 1189 661 644 637 397 198 BclI T'GATC_A Cuts at: 1548 2482 6016 7600 1548 Size: 934 3534 1584 4368 Fragments arranged by size: 4368 3534 1584 934 BfrBI ATG'CAT Cuts at: 7381 7381 Size: 10420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 6750 9031 Size: 107 122 71 631 605 377 4701 2281 Cuts at: 9031 136 Size: 1525 Fragments arranged by size: 4701 2281 1525 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 6127 7689 10415 6127 Size: 1562 2726 6132 Fragments arranged by size: 6132 2726 1562 BlpI GC'TnA_GC Cuts at: 2631 3611 7837 2631 Size: 980 4226 5214 Fragments arranged by size: 5214 4226 980 Bme1580I G_kGCm'C Cuts at: 1790 2035 3572 4219 5608 6960 8481 9637 1790 Size: 245 1537 647 1389 1352 1521 1156 2573 Fragments arranged by size: 2573 1537 1521 1389 1352 1156 647 245 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10420 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4071 5445 6135 Size: 416 53 83 399 24 2654 1374 690 Cuts at: 6135 8151 9109 442 Size: 2016 958 1753 Fragments arranged by size: 2654 2016 1753 1374 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4975 7053 7092 7127 7317 9080 1239 Size: 3736 2078 39 35 190 1763 2579 Fragments arranged by size: 3736 2579 2078 1763 190 39 35 Bpu10I CC'TnA_GC Cuts at: 4848 4889 4848 Size: 41 10379 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5795 7229 7510 7545 8545 9410 9708 5795 Size: 1434 281 35 1000 865 298 6507 Fragments arranged by size: 6507 1434 1000 865 298 281 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4991 6157 6805 7548 9083 882 Size: 617 3492 1166 648 743 1535 2219 Fragments arranged by size: 3492 2219 1535 1166 743 648 617 BsaAI yAC'GTr Cuts at: 493 1204 4575 6694 8211 493 Size: 711 3371 2119 1517 2702 Fragments arranged by size: 3371 2702 2119 1517 711 BsaBI GATnn'nnATC Cuts at: 2999 5081 6270 6418 6620 7500 10414 2999 Size: 2082 1189 148 202 880 2914 3005 Fragments arranged by size: 3005 2914 2082 1189 880 202 148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8227 8609 9244 275 Size: 53 83 186 936 6694 382 635 1451 Fragments arranged by size: 6694 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3186 8853 9251 9525 9672 10083 3186 Size: 5667 398 274 147 411 3523 Fragments arranged by size: 5667 3523 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2841 2871 7214 7244 1178 Size: 30 165 30 1438 30 4343 30 4354 Fragments arranged by size: 4354 4343 1438 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 3683 4906 5960 6330 7277 1186 Size: 829 1668 1223 1054 370 947 4329 Fragments arranged by size: 4329 1668 1223 1054 947 829 370 BseYI C'CCAG_C Cuts at: 3787 5524 5954 9623 10119 3787 Size: 1737 430 3669 496 4088 Fragments arranged by size: 4088 3669 1737 496 430 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 2481 2511 2520 5126 5877 1163 Size: 573 378 367 30 9 2606 751 5706 Fragments arranged by size: 5706 2606 751 573 378 367 30 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2126 7870 8631 9659 1540 Size: 275 186 125 5744 761 1028 2301 Fragments arranged by size: 5744 2301 1028 761 275 186 125 BsiHKAI G_wGCw'C Cuts at: 4219 4815 6960 8481 8566 9637 4219 Size: 596 2145 1521 85 1071 5002 Fragments arranged by size: 5002 2145 1521 1071 596 85 BsmI GAATG_Cn' Cuts at: 3517 3549 3568 6869 7931 8024 3517 Size: 32 19 3301 1062 93 5913 Fragments arranged by size: 5913 3301 1062 93 32 19 BsmBI CGTCTCn'nnnn_ Cuts at: 5621 5621 Size: 10420 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 3572 4219 4815 4854 5608 6960 Size: 399 245 1537 647 596 39 754 1352 Cuts at: 6960 7739 8481 8566 9637 1391 Size: 779 742 85 1071 2174 Fragments arranged by size: 2174 1537 1352 1071 779 754 742 647 596 399 245 85 39 BspEI T'CCGG_A Cuts at: 3186 10083 3186 Size: 6897 3523 BspHI T'CATG_A Cuts at: 3049 8309 10039 3049 Size: 5260 1730 3430 Fragments arranged by size: 5260 3430 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2077 4401 7775 844 Size: 1233 2324 3374 3489 Fragments arranged by size: 3489 3374 2324 1233 BsrBI CCG'CTC Cuts at: 1394 1823 8307 1394 Size: 429 6484 3507 Fragments arranged by size: 6484 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2403 3738 4747 5352 8915 9089 65 Size: 1183 1155 1335 1009 605 3563 174 1396 Fragments arranged by size: 3563 1396 1335 1183 1155 1009 605 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 6768 7203 9064 9251 1700 Size: 110 337 4621 435 1861 187 2869 Fragments arranged by size: 4621 2869 1861 435 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10420 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10420 BssSI C'ACGA_G Cuts at: 8480 9492 8480 Size: 1012 9408 BstAPI GCAn_nnn'nTGC Cuts at: 794 2092 2498 4646 794 Size: 1298 406 2148 6568 Fragments arranged by size: 6568 2148 1298 406 BstBI TT'CG_AA Cuts at: 5002 9224 5002 Size: 4222 6198 BstEII G'GTnAC_C Cuts at: 1184 3205 1184 Size: 2021 8399 BstYI r'GATC_y Cuts at: 1972 2302 2454 3704 3943 5282 6127 6891 7689 Size: 330 152 1250 239 1339 845 764 798 Cuts at: 7689 7750 8117 8502 8519 9971 10057 10069 10415 Size: 61 367 385 17 1452 86 12 346 Cuts at: 10415 1972 Size: 1977 Fragments arranged by size: 1977 1452 1339 1250 845 798 764 385 367 346 330 239 152 86 61 17 12 BstZ17I GTA'TAC Cuts at: 6435 6435 Size: 10420 Bsu36I CC'TnA_GG Cuts at: 3394 3888 4759 3394 Size: 494 871 9055 Fragments arranged by size: 9055 871 494 BtgI C'CryG_G Cuts at: 513 1065 2044 7653 513 Size: 552 979 5609 3280 Fragments arranged by size: 5609 3280 979 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9681 Fragments arranged by size: 9681 683 56 BtsI GCAGTG_nn' Cuts at: 3483 6470 7936 8730 8750 3483 Size: 2987 1466 794 20 5153 Fragments arranged by size: 5153 2987 1466 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10420 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10385 DraI TTT'AAA Cuts at: 3081 3663 4584 7724 3081 Size: 582 921 3140 5777 Fragments arranged by size: 5777 3140 921 582 DraIII CAC_nnn'GTG Cuts at: 3559 6143 3559 Size: 2584 7836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6552 9427 809 Size: 507 5236 2875 1802 Fragments arranged by size: 5236 2875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 5599 7201 Size: 54 1425 50 275 18 293 3476 1602 Cuts at: 7201 7867 8756 10385 8 Size: 666 889 1629 43 Fragments arranged by size: 3476 1629 1602 1425 889 666 293 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2123 7867 1537 Size: 275 311 5744 4090 Fragments arranged by size: 5744 4090 311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 2778 3695 4657 6155 6724 6825 8350 Size: 999 426 917 962 1498 569 101 1525 Cuts at: 8350 1353 Size: 3423 Fragments arranged by size: 3423 1525 1498 999 962 917 569 426 101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6732 9007 9381 9527 387 Size: 1064 138 5143 2275 374 146 1280 Fragments arranged by size: 5143 2275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4813 4813 Size: 10420 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3715 4181 4442 4844 4975 5001 5319 Size: 393 2083 466 261 402 131 26 318 Cuts at: 5319 7053 7092 7127 7317 7391 7601 8483 9080 Size: 1734 39 35 190 74 210 882 597 Cuts at: 9080 9867 10135 1239 Size: 787 268 1524 Fragments arranged by size: 2083 1734 1524 882 787 597 466 402 393 318 268 261 210 190 131 74 39 35 26 EcoNI CCTnn'n_nnAGG Cuts at: 2084 4734 5538 6925 2084 Size: 2650 804 1387 5579 Fragments arranged by size: 5579 2650 1387 804 EcoO109I rG'GnC_Cy Cuts at: 2333 3874 5605 6673 6761 2333 Size: 1541 1731 1068 88 5992 Fragments arranged by size: 5992 1731 1541 1068 88 EcoRI G'AATT_C Cuts at: 3797 6078 7729 8107 3797 Size: 2281 1651 378 6110 Fragments arranged by size: 6110 2281 1651 378 EcoRV GAT'ATC Cuts at: 5470 6319 7669 5470 Size: 849 1350 8221 Fragments arranged by size: 8221 1350 849 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3264 4076 4496 3264 Size: 812 420 9188 Fragments arranged by size: 9188 812 420 FspI TGC'GCA Cuts at: 8926 8926 Size: 10420 HaeII r_GCGC'y Cuts at: 1678 3088 9567 1678 Size: 1410 6479 2531 Fragments arranged by size: 6479 2531 1410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2439 2471 3298 3330 4929 Size: 32 135 32 867 32 827 32 1599 Cuts at: 4929 4961 4969 5001 5149 5181 6008 6040 6052 Size: 32 8 32 148 32 827 32 12 Cuts at: 6052 6084 6119 6151 6365 6397 6510 6542 6635 Size: 32 35 32 214 32 113 32 93 Cuts at: 6635 6667 9061 9093 9135 9167 1373 Size: 32 2394 32 42 32 2626 Fragments arranged by size: 2626 2394 1599 867 827 827 214 148 135 113 93 42 35 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 12 8 HincII GTy'rAC Cuts at: 669 1657 1938 3245 4378 4948 5124 6450 7764 Size: 988 281 1307 1133 570 176 1326 1314 Cuts at: 7764 8010 669 Size: 246 3079 Fragments arranged by size: 3079 1326 1314 1307 1133 988 570 281 246 176 HindIII A'AGCT_T Cuts at: 748 3171 4865 748 Size: 2423 1694 6303 Fragments arranged by size: 6303 2423 1694 HpaI GTT'AAC Cuts at: 8010 8010 Size: 10420 KpnI G_GTAC'C Cuts at: 4758 7745 4758 Size: 2987 7433 MfeI C'AATT_G Cuts at: 2844 5160 6247 8019 2844 Size: 2316 1087 1772 5245 Fragments arranged by size: 5245 2316 1772 1087 MluI A'CGCG_T Cuts at: 10296 10296 Size: 10420 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 3971 4586 5000 7267 9534 9718 1305 Size: 1350 1316 615 414 2267 2267 184 2007 Fragments arranged by size: 2267 2267 2007 1350 1316 615 414 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 8598 Fragments arranged by size: 8598 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3215 3781 5370 8378 8737 8896 518 Size: 648 2049 566 1589 3008 359 159 2042 Fragments arranged by size: 3008 2049 2042 1589 648 566 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2963 5110 5524 6047 7634 7836 8513 Size: 516 841 2147 414 523 1587 202 677 Cuts at: 8513 9661 9906 1606 Size: 1148 245 2120 Fragments arranged by size: 2147 2120 1587 1148 841 677 523 516 414 245 202 NaeI GCC'GGC Cuts at: 2149 7205 2149 Size: 5056 5364 NcoI C'CATG_G Cuts at: 513 1065 7653 513 Size: 552 6588 3280 Fragments arranged by size: 6588 3280 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10420 NgoMIV G'CCGG_C Cuts at: 2147 7203 2147 Size: 5056 5364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10420 NotI GC'GGCC_GC Cuts at: 7867 7867 Size: 10420 NsiI A_TGCA'T Cuts at: 7383 7383 Size: 10420 NspI r_CATG'y Cuts at: 1132 1229 2071 3011 4286 5403 6688 7180 7385 Size: 97 842 940 1275 1117 1285 492 205 Cuts at: 7385 7439 7778 9323 1132 Size: 54 339 1545 2229 Fragments arranged by size: 2229 1545 1285 1275 1117 940 842 492 339 205 97 54 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10420 PciI A'CATG_T Cuts at: 9319 9319 Size: 10420 PflMI CCAn_nnn'nTGG Cuts at: 1108 3787 7118 1108 Size: 2679 3331 4410 Fragments arranged by size: 4410 3331 2679 PfoI T'CCnGG_A Cuts at: 6975 6975 Size: 10420 PmeI GTTT'AAAC Cuts at: 7724 7724 Size: 10420 PmlI CAC'GTG Cuts at: 4575 6694 4575 Size: 2119 8301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7253 7285 8489 8521 10002 10034 7253 Size: 32 1204 32 1481 32 7639 Fragments arranged by size: 7639 1481 1204 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2333 3874 6673 2333 Size: 1541 2799 6080 Fragments arranged by size: 6080 2799 1541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10420 PsiI TTA'TAA Cuts at: 5243 5688 7990 5243 Size: 445 2302 7673 Fragments arranged by size: 7673 2302 445 PspOMI G'GGCC_C Cuts at: 5604 5604 Size: 10420 PstI C_TGCA'G Cuts at: 830 6561 7772 830 Size: 5731 1211 3478 Fragments arranged by size: 5731 3478 1211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10420 PvuII CAG'CTG Cuts at: 2963 5110 6047 7634 2963 Size: 2147 937 1587 5749 Fragments arranged by size: 5749 2147 1587 937 SacI G_AGCT'C Cuts at: 4815 4815 Size: 10420 SalI G'TCGA_C Cuts at: 1936 6448 7762 1936 Size: 4512 1314 4594 Fragments arranged by size: 4594 4512 1314 SbfI CC_TGCA'GG Cuts at: 7772 7772 Size: 10420 ScaI AGT'ACT Cuts at: 2372 7475 8668 10380 2372 Size: 5103 1193 1712 2412 Fragments arranged by size: 5103 2412 1712 1193 SexAI A'CCwGG_T Cuts at: 6795 6795 Size: 10420 SfcI C'TryA_G Cuts at: 826 1045 2268 3293 3452 3695 6208 6557 7768 Size: 219 1223 1025 159 243 2513 349 1211 Cuts at: 7768 8903 9584 9775 826 Size: 1135 681 191 1471 Fragments arranged by size: 2513 1471 1223 1211 1135 1025 681 349 243 219 191 159 SfiI GGCCn_nnn'nGGCC Cuts at: 2049 2049 Size: 10420 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10420 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10420 SmaI CCC'GGG Cuts at: 1872 7747 1872 Size: 5875 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 4814 5810 7208 7489 7560 Size: 197 926 885 1986 996 1398 281 71 Cuts at: 7560 8524 9425 9687 820 Size: 964 901 262 1553 Fragments arranged by size: 1986 1553 1398 996 964 926 901 885 281 262 197 71 SnaBI TAC'GTA Cuts at: 493 8211 493 Size: 7718 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10420 SphI G_CATG'C Cuts at: 1132 2071 7385 7778 1132 Size: 939 5314 393 3774 Fragments arranged by size: 5314 3774 939 393 SspI AAT'ATT Cuts at: 5 52 2182 8344 5 Size: 47 2130 6162 2081 Fragments arranged by size: 6162 2130 2081 47 StuI AGG'CCT Cuts at: 1517 6744 1517 Size: 5227 5193 StyI C'CwwG_G Cuts at: 513 1065 2724 2741 3744 4321 4735 4906 7341 Size: 552 1659 17 1003 577 414 171 2435 Cuts at: 7341 7653 7662 7859 513 Size: 312 9 197 3074 Fragments arranged by size: 3074 2435 1659 1003 577 552 414 312 197 171 17 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6154 6737 7629 8447 8464 8617 8802 Size: 1255 4574 583 892 818 17 153 185 Cuts at: 8802 9247 325 Size: 445 1498 Fragments arranged by size: 4574 1498 1255 892 818 583 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2370 4441 5418 Size: 274 80 33 51 1443 393 2071 977 Cuts at: 5418 7473 8666 10378 96 Size: 2055 1193 1712 138 Fragments arranged by size: 2071 2055 1712 1443 1193 977 393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3351 3383 4827 4859 7214 7246 10380 10412 3351 Size: 32 1444 32 2355 32 3134 32 3359 Fragments arranged by size: 3359 3134 2355 1444 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10420 XbaI T'CTAG_A Cuts at: 5770 7756 5770 Size: 1986 8434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2671 5107 2671 Size: 2436 7984 XhoI C'TCGA_G Cuts at: 1943 4814 1943 Size: 2871 7549 XmaI C'CCGG_G Cuts at: 1870 7745 1870 Size: 5875 4545 XmnI GAAnn'nnTTC Cuts at: 4032 4416 4611 5654 8549 4032 Size: 384 195 1043 2895 5903 Fragments arranged by size: 5903 2895 1043 384 195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8228 276 Size: 53 83 186 936 6694 2468 Fragments arranged by size: 6694 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BbeI BplI BsiWI BstXI FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI