################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccII AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FauI FbaI FseI FspI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1369 GGTACC G^GTACC AccI 145 GTCGAC GT^[AC][GT]AC AcyI 780 GGCGCC G[GA]^CG[CT]C AhdI 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1541 GATCC GGATC(4/5) AlwNI 286 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 401 GGGCCC GGGCC^C Asp700I 840 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1369 GGTACC G^GTACC AspEI 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 780 GGCGCC GGCGC^C BbsI 1189 GAAGAC GAAGAC(2/6) BfaI 1070 CTAG C^TAG BglI 527 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 604 AGATCT A^GATCT BpuAI 1189 GAAGAC GAAGAC(2/6) BsaHI 780 GGCGCC G[GA]^CG[CT]C BsmI 747 GAATGC GAATGC(1/-1) BsrFI 783 GCCGGC [GA]^CCGG[CT] BstEII 1059 GGTCACC G^GT[ACGT]ACC BstPI 1059 GGTCACC G^GT[ACGT]ACC Cfr10I 783 GCCGGC [GA]^CCGG[CT] DraIII 1267 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 630 CCACGG C^C[GA][CT]GG Eam1105I 938 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1059 GGTCACC G^GT[ACGT]ACC EcoRV 676 GATATC GAT^ATC HaeII 780 GGCGCC [GA]GCGC^[CT] HgaI 354 GCGTC GACGC(5/10) Hin1I 780 GGCGCC G[GA]^CG[CT]C HpaI 798 GTTAAC GTT^AAC KasI 780 GGCGCC G^GCGCC KpnI 1369 GGTACC GGTAC^C MaeI 1070 CTAG C^TAG MseI 799 TTAA T^TAA MspA1I 97 CAGCGG C[AC]G^C[GT]G NaeI 783 GCCGGC GCC^GGC NarI 780 GGCGCC GG^CGCC NgoMIV 783 GCCGGC G^CCGGC PflMI 246 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 283 CTGCAG CTGCA^G SacI 1219 GAGCTC GAGCT^C SalI 145 GTCGAC G^TCGAC SfcI 283 CTGCAG C^T[GA][CT]AG SfoI 780 GGCGCC GGC^GCC SmaI 329 CCCGGG CCC^GGG SspI 585 AATATT AAT^ATT StuI 1097 AGGCCT AGG^CCT Tru9I 799 TTAA T^TAA Van91I 246 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 579 CCACCAAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 329 CCCGGG C^CCGGG XmaI 329 CCCGGG C^CCGGG XmnI 840 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 487 GAATTT [GA]^AATT[CT] 1312 AAATTC AflIII 346 ACATGT A^C[GA][CT]GT 917 ACATGT ApoI 487 GAATTT [GA]^AATT[CT] 1312 AAATTC AvaI 329 CCCGGG C^[CT]CG[GA]G 1252 CCCGAG BanI 780 GGCGCC G^G[CT][GA]CC 1369 GGTACC BsaI 219 GAGACC GGTCTC(1/5) 1229 GAGACC BsmFI 327 GTCCC GGGAC(10/14) 356 GTCCC BsoBI 329 CCCGGG C^[CT]CG[GA]G 1252 CCCGAG BspLU11I 346 ACATGT A^CATGT 917 ACATGT BstAPI 79 GCAGCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 442 GCATTGGCTGC BstXI 408 CCACAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 582 CCAAATATTTGG BstYI 604 AGATCT [GA]^GATC[CT] 1540 AGATCC CfoI 781 GCGC GCG^C 1184 GCGC EaeI 70 CGGCCA [CT]^GGCC[GA] 534 CGGCCA EcoT14I 818 CCAAGG C^C[AT][AT]GG 1179 CCTTGG HhaI 781 GCGC GCG^C 1184 GCGC HinP1I 781 GCGC G^CGC 1184 GCGC HindIII 199 AAGCTT A^AGCTT 704 AAGCTT MflI 604 AGATCT [GA]^GATC[CT] 1540 AGATCC PpuMI 821 AGGTCCT [GA]G^G[AT]CC[CT] 936 AGGACCT SmlI 1022 CTCAAG C^T[CT][GA]AG 1222 CTCAAG SphI 368 GCATGC GCATG^C 523 GCATGC StyI 818 CCAAGG C^C[AT][AT]GG 1179 CCTTGG Tsp509I 488 AATT ^AATT 1313 AATT XhoII 604 AGATCT [GA]^GATC[CT] 1540 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp45I TspRI TthHB8I