(Circular) (Six-base) MAPSORT of: pFN21AE1253.seq Check: 468 from: 1 to: 6295 pFN21AE1253 6295 bp With 182 enzymes: SgfI * March 28, 2012 12:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2151 3638 1937 Size: 214 1487 4594 Fragments arranged by size: 4594 1487 214 Acc65I G'GTAC_C Cuts at: 3374 3616 3374 Size: 242 6053 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4358 5742 6010 1632 Size: 2726 1384 268 1917 Fragments arranged by size: 2726 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 2351 2922 5194 6171 1203 Size: 1148 571 2272 977 1327 Fragments arranged by size: 2272 1327 1148 977 571 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 2948 5024 2948 Size: 2076 4219 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2499 1184 Size: 1315 4980 AlwNI CAG_nnn'CTG Cuts at: 2296 4038 5610 2296 Size: 1742 1572 2981 Fragments arranged by size: 2981 1742 1572 ApaI G_GGCC'C Cuts at: 2410 2410 Size: 6295 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2492 3317 3604 3830 3982 6091 1493 Size: 999 825 287 226 152 2109 1697 Fragments arranged by size: 2109 1697 999 825 287 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2334 3257 3620 1870 Size: 73 391 923 363 4545 Fragments arranged by size: 4545 923 391 363 73 BamHI G'GATC_C Cuts at: 3625 3992 3625 Size: 367 5928 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2785 3374 3616 5071 618 Size: 325 217 627 998 589 242 1455 1842 Fragments arranged by size: 1842 1455 998 627 589 325 242 217 BanII G_rGCy'C Cuts at: 1391 2250 2410 3184 3228 3580 3614 1391 Size: 859 160 774 44 352 34 4072 Fragments arranged by size: 4072 859 774 352 160 44 34 BbeI G_GCGC'C Cuts at: 2789 2789 Size: 6295 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3201 928 Size: 971 1302 4022 Fragments arranged by size: 4022 1302 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2538 4906 136 Size: 107 122 71 631 605 866 2368 1525 Fragments arranged by size: 2368 1525 866 631 605 122 107 71 BglII A'GATC_T Cuts at: 2609 6290 2609 Size: 3681 2614 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 2410 4356 5512 1790 Size: 620 1946 1156 2573 Fragments arranged by size: 2573 1946 1156 620 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4026 4984 442 Size: 416 53 83 399 24 2609 958 1753 Fragments arranged by size: 2609 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2626 2762 3233 4955 1239 Size: 995 392 136 471 1722 2579 Fragments arranged by size: 2579 1722 995 471 392 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3012 3212 4420 5285 5583 3012 Size: 200 1208 865 298 3724 Fragments arranged by size: 3724 1208 865 298 200 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2218 3228 4958 882 Size: 617 719 1010 1730 2219 Fragments arranged by size: 2219 1730 1010 719 617 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2786 4102 4484 5119 Size: 53 83 186 936 1253 1316 382 635 Cuts at: 5119 275 Size: 1451 Fragments arranged by size: 1451 1316 1253 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4728 5126 5400 5547 5958 4728 Size: 398 274 147 411 5065 Fragments arranged by size: 5065 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3367 3397 1178 Size: 30 165 30 1964 30 4076 Fragments arranged by size: 4076 1964 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6295 BseYI C'CCAG_C Cuts at: 2243 2981 5498 5994 2243 Size: 738 2517 496 2544 Fragments arranged by size: 2544 2517 738 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5722 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2343 3228 3273 4356 4441 5512 2343 Size: 885 45 1083 85 1071 3126 Fragments arranged by size: 3126 1083 1071 885 85 45 BsmI GAATG_Cn' Cuts at: 2758 3806 3899 2758 Size: 1048 93 5154 Fragments arranged by size: 5154 1048 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2343 2410 3184 3228 3273 3580 Size: 399 460 93 67 774 44 45 307 Cuts at: 3580 3614 4356 4441 5512 1391 Size: 34 742 85 1071 2174 Fragments arranged by size: 2174 1071 774 742 460 399 307 93 85 67 45 44 34 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2276 2539 3650 844 Size: 1392 40 263 1111 3489 Fragments arranged by size: 3489 1392 1111 263 40 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4790 4964 65 Size: 1183 3542 174 1396 Fragments arranged by size: 3542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2788 4939 5126 1700 Size: 110 978 2151 187 2869 Fragments arranged by size: 2869 2151 978 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2090 2453 794 Size: 1296 363 4636 Fragments arranged by size: 4636 1296 363 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 3064 1184 Size: 1880 4415 BstXI CCAn_nnnn'nTGG Cuts at: 2420 2594 2420 Size: 174 6121 BstYI r'GATC_y Cuts at: 1972 2609 3545 3625 3992 4377 4394 5846 5932 Size: 637 936 80 367 385 17 1452 86 Cuts at: 5932 5944 6290 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 936 637 385 367 346 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2635 513 Size: 552 939 631 4173 Fragments arranged by size: 4173 939 631 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2656 487 Size: 683 56 1430 4126 Fragments arranged by size: 4126 1430 683 56 BtsI GCAGTG_nn' Cuts at: 2297 2629 3811 4605 4625 2297 Size: 332 1182 794 20 3967 Fragments arranged by size: 3967 1182 794 332 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DraIII CAC_nnn'GTG Cuts at: 3277 3277 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2075 2539 3742 Size: 54 1425 50 275 18 245 464 1203 Cuts at: 3742 4631 6260 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1203 889 464 275 245 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2840 3122 3478 4225 1353 Size: 1487 282 356 747 3423 Fragments arranged by size: 3423 1487 747 356 282 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3226 3226 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2626 2762 3233 4358 4955 5742 Size: 393 602 392 136 471 1125 597 787 Cuts at: 5742 6010 1239 Size: 268 1524 Fragments arranged by size: 1524 1125 787 602 597 471 393 392 268 136 EcoO109I rG'GnC_Cy Cuts at: 2407 2827 2942 3253 2407 Size: 420 115 311 5449 Fragments arranged by size: 5449 420 311 115 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 EcoRV GAT'ATC Cuts at: 2683 2683 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 2789 5442 1678 Size: 1111 2653 2531 Fragments arranged by size: 2653 2531 1111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 2601 2633 2934 Size: 32 135 32 974 32 23 32 301 Cuts at: 2934 2966 3143 3175 4936 4968 5010 5042 1373 Size: 32 177 32 1761 32 42 32 2626 Fragments arranged by size: 2626 1761 974 301 177 135 42 32 32 32 32 32 32 32 32 23 HincII GTy'rAC Cuts at: 669 1657 1938 2152 2805 3098 3639 3885 669 Size: 988 281 214 653 293 541 246 3079 Fragments arranged by size: 3079 988 653 541 293 281 246 214 HindIII A'AGCT_T Cuts at: 748 2204 2709 748 Size: 1456 505 4334 Fragments arranged by size: 4334 1456 505 HpaI GTT'AAC Cuts at: 2805 3885 2805 Size: 1080 5215 KasI G'GCGC_C Cuts at: 2785 2785 Size: 6295 KpnI G_GTAC'C Cuts at: 3378 3620 3378 Size: 242 6053 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3243 5409 5593 1305 Size: 1938 2166 184 2007 Fragments arranged by size: 2166 2007 1938 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4473 Fragments arranged by size: 4473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4253 4612 4771 518 Size: 648 3087 359 159 2042 Fragments arranged by size: 3087 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2104 3711 4388 5536 5781 1606 Size: 498 1607 677 1148 245 2120 Fragments arranged by size: 2120 1607 1148 677 498 245 NaeI GCC'GGC Cuts at: 2790 2790 Size: 6295 NarI GG'CG_CC Cuts at: 2786 2786 Size: 6295 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4804 Fragments arranged by size: 4804 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NgoMIV G'CCGG_C Cuts at: 2788 2788 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2329 2355 2377 2532 2914 2926 3653 Size: 97 1100 26 22 155 382 12 727 Cuts at: 3653 5198 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1100 727 382 155 97 26 22 12 PasI CC'CwG_GG Cuts at: 1348 2196 1348 Size: 848 5447 PciI A'CATG_T Cuts at: 2351 2922 5194 2351 Size: 571 2272 3452 Fragments arranged by size: 3452 2272 571 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 1108 Size: 1149 5146 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2027 2059 4364 4396 5877 5909 2027 Size: 32 2305 32 1481 32 2413 Fragments arranged by size: 2413 2305 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 2942 2827 Size: 115 6180 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspOMI G'GGCC_C Cuts at: 2406 2406 Size: 6295 PstI C_TGCA'G Cuts at: 830 2292 3647 830 Size: 1462 1355 3478 Fragments arranged by size: 3478 1462 1355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 SacI G_AGCT'C Cuts at: 3228 3228 Size: 6295 SalI G'TCGA_C Cuts at: 1936 2150 3637 1936 Size: 214 1487 4594 Fragments arranged by size: 4594 1487 214 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2288 3643 4778 5459 5650 826 Size: 219 1243 1355 1135 681 191 1471 Fragments arranged by size: 1471 1355 1243 1135 681 219 191 SfoI GGC'GCC Cuts at: 2787 2787 Size: 6295 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 2336 3622 1872 Size: 464 1286 4545 Fragments arranged by size: 4545 1286 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 3027 3227 4399 5300 5562 820 Size: 197 926 1084 200 1172 901 262 1553 Fragments arranged by size: 1553 1172 1084 926 901 262 200 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 2377 2532 3653 1132 Size: 1245 155 1121 3774 Fragments arranged by size: 3774 1245 1121 155 SspI AAT'ATT Cuts at: 5 52 2592 4219 5 Size: 47 2540 1627 2081 Fragments arranged by size: 2540 2081 1627 47 StuI AGG'CCT Cuts at: 1517 3104 1517 Size: 1587 4708 StyI C'CwwG_G Cuts at: 513 1065 2004 2823 3184 3734 513 Size: 552 939 819 361 550 3074 Fragments arranged by size: 3074 939 819 552 550 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2973 4322 4339 4492 4677 5122 325 Size: 1255 1393 1349 17 153 185 445 1498 Fragments arranged by size: 1498 1393 1349 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2381 4541 6253 Size: 274 80 33 51 1443 404 2160 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 2160 1712 1443 404 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6255 6287 6255 Size: 32 6263 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2591 2591 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 2334 3620 1870 Size: 464 1286 4545 Fragments arranged by size: 4545 1286 464 XmnI GAAnn'nnTTC Cuts at: 2849 4424 2849 Size: 1575 4720 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI