Restriction map for
pFN21AE1253
(Linear) (Six-base) MAPSORT of: KIEE1253.seq Check: 3682 from: 1 to: 1590
KIEE1253.orf
With 182 enzymes: SgfI *
March 22, 2012 15:51 ..
AccI GT'mk_AC
Cuts at: 0 146 1590
Size: 146 1444
Acc65I G'GTAC_C
Cuts at: 0 1369 1590
Size: 1369 221
AflIII A'CryG_T
Cuts at: 0 346 917 1590
Size: 346 571 673
Fragments arranged by size:
673 571 346
AhdI GACnn_n'nnGTC
Cuts at: 0 943 1590
Size: 943 647
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 494 1590
Size: 494 1096
AlwNI CAG_nnn'CTG
Cuts at: 0 291 1590
Size: 291 1299
ApaI G_GGCC'C
Cuts at: 0 405 1590
Size: 405 1185
ApoI r'AATT_y
Cuts at: 0 487 1312 1590
Size: 487 825 278
Fragments arranged by size:
825 487 278
AvaI C'yCGr_G
Cuts at: 0 329 1252 1590
Size: 329 923 338
Fragments arranged by size:
923 338 329
BanI G'GyrC_C
Cuts at: 0 780 1369 1590
Size: 780 589 221
Fragments arranged by size:
780 589 221
BanII G_rGCy'C
Cuts at: 0 245 405 1179 1223 1575 1590
Size: 245 160 774 44 352 15
Fragments arranged by size:
774 352 245 160 44 15
BbeI G_GCGC'C
Cuts at: 0 784 1590
Size: 784 806
BbsI GAAGACnn'nnnn_
Cuts at: 0 1196 1590
Size: 1196 394
BglI GCCn_nnn'nGGC
Cuts at: 0 533 1590
Size: 533 1057
BglII A'GATC_T
Cuts at: 0 604 1590
Size: 604 986
Bme1580I G_kGCm'C
Cuts at: 0 405 1590
Size: 405 1185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1590
Size: 27 1563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 621 757 1228 1590
Size: 229 392 136 471 362
Fragments arranged by size:
471 392 362 229 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1207 1590
Size: 1007 200 383
Fragments arranged by size:
1007 383 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 1223 1590
Size: 213 1010 367
Fragments arranged by size:
1010 367 213
BsaHI Gr'CG_yC
Cuts at: 0 781 1590
Size: 781 809
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1362 1392 1590
Size: 1362 30 198
Fragments arranged by size:
1362 198 30
BseYI C'CCAG_C
Cuts at: 0 238 976 1590
Size: 238 738 614
Fragments arranged by size:
738 614 238
BsiHKAI G_wGCw'C
Cuts at: 0 338 1223 1268 1590
Size: 338 885 45 322
Fragments arranged by size:
885 338 322 45
BsmI GAATG_Cn'
Cuts at: 0 753 1590
Size: 753 837
Bsp1286I G_dGCh'C
Cuts at: 0 245 338 405 1179 1223 1268 1575 1590
Size: 245 93 67 774 44 45 307 15
Fragments arranged by size:
774 307 245 93 67 45 44 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 231 271 534 1590
Size: 231 40 263 1056
Fragments arranged by size:
1056 263 231 40
BsrFI r'CCGG_y
Cuts at: 0 783 1590
Size: 783 807
BstAPI GCAn_nnn'nTGC
Cuts at: 0 85 448 1590
Size: 85 363 1142
Fragments arranged by size:
1142 363 85
BstEII G'GTnAC_C
Cuts at: 0 1059 1590
Size: 1059 531
BstXI CCAn_nnnn'nTGG
Cuts at: 0 415 589 1590
Size: 415 174 1001
Fragments arranged by size:
1001 415 174
BstYI r'GATC_y
Cuts at: 0 604 1540 1590
Size: 604 936 50
Fragments arranged by size:
936 604 50
BtgI C'CryG_G
Cuts at: 0 630 1590
Size: 630 960
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 651 1590
Size: 651 939
BtsI GCAGTG_nn'
Cuts at: 0 292 624 1590
Size: 292 332 966
Fragments arranged by size:
966 332 292
DraIII CAC_nnn'GTG
Cuts at: 0 1272 1590
Size: 1272 318
EaeI y'GGCC_r
Cuts at: 0 70 534 1590
Size: 70 464 1056
Fragments arranged by size:
1056 464 70
EarI CTCTTCn'nnn_
Cuts at: 0 835 1117 1473 1590
Size: 835 282 356 117
Fragments arranged by size:
835 356 282 117
EcoICRI GAG'CTC
Cuts at: 0 1221 1590
Size: 1221 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 621 757 1228 1590
Size: 229 392 136 471 362
Fragments arranged by size:
471 392 362 229 136
EcoO109I rG'GnC_Cy
Cuts at: 0 402 822 937 1248 1590
Size: 402 420 115 311 342
Fragments arranged by size:
420 402 342 311 115
EcoRV GAT'ATC
Cuts at: 0 678 1590
Size: 678 912
HaeII r_GCGC'y
Cuts at: 0 784 1590
Size: 784 806
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 596 628 929 961 1138 1170
Size: 541 32 23 32 301 32 177 32
Cuts at: 1170 1590
Size: 420
Fragments arranged by size:
541 420 301 177 32 32 32 32
23
HincII GTy'rAC
Cuts at: 0 147 800 1093 1590
Size: 147 653 293 497
Fragments arranged by size:
653 497 293 147
HindIII A'AGCT_T
Cuts at: 0 199 704 1590
Size: 199 505 886
Fragments arranged by size:
886 505 199
HpaI GTT'AAC
Cuts at: 0 800 1590
Size: 800 790
KasI G'GCGC_C
Cuts at: 0 780 1590
Size: 780 810
KpnI G_GTAC'C
Cuts at: 0 1373 1590
Size: 1373 217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1238 1590
Size: 1238 352
MspA1I CmG'CkG
Cuts at: 0 99 1590
Size: 99 1491
NaeI GCC'GGC
Cuts at: 0 785 1590
Size: 785 805
NarI GG'CG_CC
Cuts at: 0 781 1590
Size: 781 809
NgoMIV G'CCGG_C
Cuts at: 0 783 1590
Size: 783 807
NspI r_CATG'y
Cuts at: 0 324 350 372 527 909 921 1590
Size: 324 26 22 155 382 12 669
Fragments arranged by size:
669 382 324 155 26 22 12
PasI CC'CwG_GG
Cuts at: 0 191 1590
Size: 191 1399
PciI A'CATG_T
Cuts at: 0 346 917 1590
Size: 346 571 673
Fragments arranged by size:
673 571 346
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1590
Size: 252 1338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 1590
Size: 22 32 1536
Fragments arranged by size:
1536 32 22
PpuMI rG'GwC_Cy
Cuts at: 0 822 937 1590
Size: 822 115 653
Fragments arranged by size:
822 653 115
PspOMI G'GGCC_C
Cuts at: 0 401 1590
Size: 401 1189
PstI C_TGCA'G
Cuts at: 0 287 1590
Size: 287 1303
SacI G_AGCT'C
Cuts at: 0 1223 1590
Size: 1223 367
SalI G'TCGA_C
Cuts at: 0 145 1590
Size: 145 1445
SfcI C'TryA_G
Cuts at: 0 283 1590
Size: 283 1307
SfoI GGC'GCC
Cuts at: 0 782 1590
Size: 782 808
SmaI CCC'GGG
Cuts at: 0 331 1590
Size: 331 1259
SmlI C'TyrA_G
Cuts at: 0 1022 1222 1590
Size: 1022 200 368
Fragments arranged by size:
1022 368 200
SphI G_CATG'C
Cuts at: 0 372 527 1590
Size: 372 155 1063
Fragments arranged by size:
1063 372 155
SspI AAT'ATT
Cuts at: 0 587 1590
Size: 587 1003
StuI AGG'CCT
Cuts at: 0 1099 1590
Size: 1099 491
StyI C'CwwG_G
Cuts at: 0 818 1179 1590
Size: 818 361 411
Fragments arranged by size:
818 411 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 968 1590
Size: 968 622
TatI w'GTAC_w
Cuts at: 0 376 1590
Size: 376 1214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 586 1590
Size: 586 1004
XmaI C'CCGG_G
Cuts at: 0 329 1590
Size: 329 1261
XmnI GAAnn'nnTTC
Cuts at: 0 844 1590
Size: 844 746
Enzymes that do cut:
AccI Acc65I AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I
BplI BpmI BpuEI BsaI BsaHI BsaXI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV HaeII Hin4I HincII HindIII HpaI KasI
KpnI MmeI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PflMI PpiI PpuMI PspOMI PstI SacI SalI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI Bpu10I BsaAI BsaBI BsaWI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EciI EcoNI EcoRI FalI FseI FspI FspAI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI TstI Tth111I XbaI XhoI ZraI