Restriction map for
pFN21AE6732
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII BbeI
BbrPI BbvCI BcgI BfrI BglI
BlpI Bpu1102I BsaAI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HaeII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 2306 GTAGAC GT^[AC][GT]AC
AcyI 691 GACGCC G[GA]^CG[CT]C
AflIII 1110 ACGTGT A^C[GA][CT]GT
AseI 3150 ATTAAT AT^TAAT
AsnI 3150 ATTAAT AT^TAAT
Asp700I 2265 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 1023 TGATCA T^GATCA
BcnI 2380 CCGGG CC^[GC]GG
BsaBI 1312 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 691 GACGCC G[GA]^CG[CT]C
BsaWI 2101 ACCGGA [AT]^CCGG[AT]
BseRI 2015 CTCCTC GAGGAG(10/8)
BsgI 2120 GTGCAG GTGCAG(16/14)
Bsp1407I 1227 TGTACA T^GTACA
BsrGI 1227 TGTACA T^GTACA
CfoI 2063 GCGC GCG^C
DraII 944 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 1390 GACTTTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 731 GAAGAG CTCTTC(1/4)
EcoO109I 944 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 3241 ATGCAT ATGCA^T
FbaI 1023 TGATCA T^GATCA
HgaI 691 GACGC GACGC(5/10)
HhaI 2063 GCGC GCG^C
Hin1I 691 GACGCC G[GA]^CG[CT]C
HinP1I 2063 GCGC G^CGC
HindIII 222 AAGCTT A^AGCTT
Ksp632I 731 GAAGAG CTCTTC(1/4)
MaeII 1110 ACGT A^CGT
MamI 1312 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 2380 CCGGG CC^[GC]GG
NsiI 3241 ATGCAT ATGCA^T
NspI 2163 GCATGT [GA]CATG^[CT]
PflMI 524 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3150 ATTAAT AT^TAAT
SfcI 2146 CTACAG C^T[GA][CT]AG
SpeI 2298 ACTAGT A^CTAGT
SspBI 1227 TGTACA T^GTACA
Van91I 524 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2265 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AciI 1835 CCGC CCGC(-3/-1)
2806 CCGC
AlwNI 161 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2252 CAGCATCTG
AvrII 60 CCTAGG C^CTAGG
2877 CCTAGG
BamHI 2417 GGATCC G^GATCC
3350 GGATCC
BanII 924 GGGCTC G[GA]GC[CT]^C
3384 GGGCTC
BciVI 1305 GTATCC GTATCC(6/5)
1317 GTATCC
BglII 1075 AGATCT A^GATCT
2087 AGATCT
BlnI 60 CCTAGG C^CTAGG
2877 CCTAGG
BspHI 1026 TCATGA T^CATGA
2792 TCATGA
BstXI 207 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1867 CCACTCTCCTGG
DraIII 1329 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1843 CACTGTGTG
EcoRI 77 GAATTC G^AATTC
509 GAATTC
HapII 2102 CCGG C^CGG
2380 CCGG
HpaII 2102 CCGG C^CGG
2380 CCGG
MfeI 1852 CAATTG C^AATTG
2485 CAATTG
MspI 2102 CCGG C^CGG
2380 CCGG
MunI 1852 CAATTG C^AATTG
2485 CAATTG
PvuII 286 CAGCTG CAG^CTG
2346 CAGCTG
RcaI 1026 TCATGA T^CATGA
2792 TCATGA
SmlI 622 CTCAAG C^T[CT][GA]AG
927 CTCAAG
XbaI 121 TCTAGA T^CTAGA
133 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BalI BanI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII