##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        Sau3AI      
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

ApaI           71  GGGCCC               GGGCC^C
AspHI         106  GAGCTC               G[AT]GC[AT]^C
AspI          282  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           37  CTCGGG               C^[CT]CG[GA]G
BglI          300  GCCCCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          136  CTCCAG               CTGGAG(16/14)
BsaI          287  GGTCTC               GGTCTC(1/5)
BseRI          34  CTCCTC               GAGGAG(10/8)
BsiHKAI       106  GAGCTC               G[AT]GC[AT]^C
BsmAI         288  GTCTC                GTCTC(1/5)
BsoBI          37  CTCGGG               C^[CT]CG[GA]G
HaeII         331  GGCGCT               [GA]GCGC^[CT]
HgaI          181  GACGC                GACGC(5/10)
HphI           98  TCACC                GGTGA(8/7)
MaeII          94  ACGT                 A^CGT
NspI          245  ACATGC               [GA]CATG^[CT]
PflFI         282  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         280  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         326  CAGCTG               CAG^CTG
SacI          106  GAGCTC               GAGCT^C
SbfI          255  CCTGCAGG             CCTGCA^GG
SexAI         283  ACCTGGT              A^CC[AT]GGT
SfaNI         267  GATGC                GCATC(5/9)
Sse8387I      255  CCTGCAGG             CCTGCA^GG
StuI          260  AGGCCT               AGG^CCT
Tth111I       282  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AluI          107  AGCT                 AG^CT
              327  AGCT                
AlwNI         139  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              151  CAGGGCCTG           
BcnI            8  CCCGG                CC^[GC]GG
               74  CCCGG               
BlpI          108  GCTCAGC              GC^T[ACGT]AGC
              210  GCTCAGC             
Bpu1102I      108  GCTCAGC              GC^T[ACGT]AGC
              210  GCTCAGC             
BsmFI          91  GGGAC                GGGAC(10/14)
              280  GGGAC               
BssHII         52  GCGCGC               G^CGCGC
              128  GCGCGC              
BstF5I        252  CATCC                GGATG(2/0)
              266  GGATG               
CelII         108  GCTCAGC              GC^T[ACGT]AGC
              210  GCTCAGC             
EcoNI         117  CCTGCGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              200  CCTGGACCAGG         
FokI          252  CATCC                GGATG(9/13)
              266  GGATG               
HapII           9  CCGG                 C^CGG
               75  CCGG                
HpaII           9  CCGG                 C^CGG
               75  CCGG                
MspA1I        139  CAGCGG               C[AC]G^C[GT]G
              326  CAGCTG              
MspI            9  CCGG                 C^CGG
               75  CCGG                
NciI            8  CCCGG                CC^[GC]GG
               74  CCCGG               
NlaIII        195  CATG                 CATG^
              246  CATG                
PstI          256  CTGCAG               CTGCA^G
              338  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AccII       AciI        AvaII       BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EcoO109I    EcoRII      
FauI        Fnu4HI      HaeIII      HhaI        HinP1I      
ItaI        MnlI        MvaI        MvnI        MwoI        
NlaIV       Sau96I      ScrFI       SfcI        TseI