Restriction map for
pFN21AE5724
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbrPI BciVI BfrI BlnI
BsaAI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eam1105I EcoO65I EcoRV EcoT22I FseI
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 970 GACGTC GACGT^C
AccI 598 GTCTAC GT^[AC][GT]AC
AcsI 1650 GAATTC [GA]^AATT[CT]
AflIII 1109 ACATGT A^C[GA][CT]GT
Aor51HI 1670 AGCGCT AGC^GCT
ApoI 1650 GAATTC [GA]^AATT[CT]
AviII 506 TGCGCA TGC^GCA
BamHI 1462 GGATCC G^GATCC
BbeI 1435 GGCGCC GGCGC^C
BcgI 1181 GCAAGATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 293 TGATCA T^GATCA
BglI 2259 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 275 TCCGGT [AT]^CCGG[AT]
BsgI 2035 CTGCAC GTGCAG(16/14)
BspLU11I 1109 ACATGT A^CATGT
BsrDI 1863 CATTGC GCAATG(2/0)
EagI 16 CGGCCG C^GGCCG
EclXI 16 CGGCCG C^GGCCG
Eco47III 1670 AGCGCT AGC^GCT
Eco52I 16 CGGCCG C^GGCCG
EcoRI 1650 GAATTC G^AATTC
FbaI 293 TGATCA T^GATCA
FspI 506 TGCGCA TGC^GCA
KasI 1435 GGCGCC G^GCGCC
MseI 1244 TTAA T^TAA
NarI 1435 GGCGCC GG^CGCC
NruI 1230 TCGCGA TCG^CGA
PflMI 1050 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1838 CAGCTG CAG^CTG
SacI 1778 GAGCTC GAGCT^C
SexAI 511 ACCTGGT A^CC[AT]GGT
SfoI 1435 GGCGCC GGC^GCC
SmlI 1576 CTTGAG C^T[CT][GA]AG
SphI 962 GCATGC GCATG^C
Tru9I 1244 TTAA T^TAA
Tsp509I 1651 AATT ^AATT
Van91I 1050 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 854 CCATCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 98 CCTCAGG CC^T[ACGT]AGG
2222 CCTCAGG
BbsI 382 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BglII 707 AGATCT A^GATCT
1478 AGATCT
BpuAI 382 GTCTTC GAAGAC(2/6)
1342 GTCTTC
BsiEI 16 CGGCCG CG[GA][CT]^CG
89 CGACCG
BsrBI 226 GAGCGG CCGCTC(-3/-3)
2209 GAGCGG
BsrFI 18 GCCGGC [GA]^CCGG[CT]
1499 GCCGGC
BstAPI 1407 GCATGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1716 GCACTTGGTGC
BstXI 734 CCAGTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1798 CCAGTACTGTGG
Bsu36I 98 CCTCAGG CC^T[ACGT]AGG
2222 CCTCAGG
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
1499 GCCGGC
DraIII 862 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1717 CACTTGGTG
DrdI 589 GACGCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
841 GACTGCTGGGTC
EarI 534 CTCTTC CTCTTC(1/4)
1062 CTCTTC
Eco81I 98 CCTCAGG CC^T[ACGT]AGG
2222 CCTCAGG
HincII 169 GTCAAC GT[CT]^[GA]AC
523 GTTGAC
HindII 169 GTCAAC GT[CT]^[GA]AC
523 GTTGAC
Ksp632I 534 CTCTTC CTCTTC(1/4)
1062 CTCTTC
MaeII 389 ACGT A^CGT
971 ACGT
NaeI 18 GCCGGC GCC^GGC
1499 GCCGGC
NcoI 1619 CCATGG C^CATGG
1990 CCATGG
NgoMIV 18 GCCGGC G^CCGGC
1499 GCCGGC
ScaI 1157 AGTACT AGT^ACT
1800 AGTACT
TaqI 218 TCGA T^CGA
1190 TCGA
TthHB8I 218 TCGA T^CGA
1190 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TseI Tsp45I TspRI XhoII XmaCI
XmaI