(Circular) (Six-base) MAPSORT of: pFN21AE5724.seq Check: 2491 from: 1 to: 7027 pFN21AE5724 7027 bp With 182 enzymes: SgfI * February 20, 2021 22:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3091 4036 3091 Size: 945 6082 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2979 4837 278 Size: 53 83 186 936 1443 1858 2468 Fragments arranged by size: 2468 1858 1443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2604 4370 1937 Size: 667 1766 4594 Fragments arranged by size: 4594 1766 667 Acc65I G'GTAC_C Cuts at: 4348 4348 Size: 7027 AclI AA'CG_TT Cuts at: 1563 5154 5527 1563 Size: 3591 373 3063 Fragments arranged by size: 3591 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3821 5090 6474 6742 1632 Size: 2189 1269 1384 268 1917 Fragments arranged by size: 2189 1917 1384 1269 268 AfeI AGC'GCT Cuts at: 3677 3677 Size: 7027 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6830 AflIII A'CryG_T Cuts at: 1203 3114 5926 6903 1203 Size: 1911 2812 977 1327 Fragments arranged by size: 2812 1911 1327 977 AgeI A'CCGG_T Cuts at: 5858 5858 Size: 7027 AhdI GACnn_n'nnGTC Cuts at: 5756 5756 Size: 7027 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7027 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7027 AlwNI CAG_nnn'CTG Cuts at: 2815 3061 3848 4283 4770 6342 2815 Size: 246 787 435 487 1572 3500 Fragments arranged by size: 3500 1572 787 487 435 246 ApaI G_GGCC'C Cuts at: 2623 3772 3893 2623 Size: 1149 121 5757 Fragments arranged by size: 5757 1149 121 ApaLI G'TGCA_C Cuts at: 5084 6240 5084 Size: 1156 5871 ApoI r'AATT_y Cuts at: 1493 3655 4336 4562 4714 6823 1493 Size: 2162 681 226 152 2109 1697 Fragments arranged by size: 2162 2109 1697 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 5581 160 Size: 872 4549 1606 Fragments arranged by size: 4549 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 AvaI C'yCGr_G Cuts at: 1870 1943 3444 3750 3772 4056 4125 4299 4352 Size: 73 1501 306 22 284 69 174 53 Cuts at: 4352 1870 Size: 4545 Fragments arranged by size: 4545 1501 306 284 174 73 69 53 22 BamHI G'GATC_C Cuts at: 3467 4357 4724 3467 Size: 890 367 5770 Fragments arranged by size: 5770 890 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2145 2482 2818 2956 Size: 325 217 627 281 77 337 336 138 Cuts at: 2956 3008 3440 3976 4348 5803 618 Size: 52 432 536 372 1455 1842 Fragments arranged by size: 1842 1455 627 536 432 372 337 336 325 281 217 138 77 52 BanII G_rGCy'C Cuts at: 1391 2623 3383 3772 3787 3893 4236 4346 1391 Size: 1232 760 389 15 106 343 110 4072 Fragments arranged by size: 4072 1232 760 389 343 110 106 15 BbeI G_GCGC'C Cuts at: 3444 3444 Size: 7027 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2380 3340 928 Size: 971 481 960 4615 Fragments arranged by size: 4615 971 960 481 BbvCI CC'TCA_GC Cuts at: 2598 2660 4164 2598 Size: 62 1504 5461 Fragments arranged by size: 5461 1504 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3175 3209 5218 5252 1903 Size: 34 1238 34 2009 34 3678 Fragments arranged by size: 3678 2009 1238 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4919 6129 4919 Size: 1210 5817 BclI T'GATC_A Cuts at: 1548 2298 1548 Size: 750 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4270 5638 Size: 107 122 71 631 605 337 2261 1368 Cuts at: 5638 136 Size: 1525 Fragments arranged by size: 2261 1525 1368 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2712 3483 7022 2712 Size: 771 3539 2717 Fragments arranged by size: 3539 2717 771 BlpI GC'TnA_GC Cuts at: 2993 3221 3786 3949 4444 2993 Size: 228 565 163 495 5576 Fragments arranged by size: 5576 565 495 228 163 Bme1580I G_kGCm'C Cuts at: 1790 2623 2823 3772 3893 3981 5088 6244 1790 Size: 833 200 949 121 88 1107 1156 2573 Fragments arranged by size: 2573 1156 1107 949 833 200 121 88 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7027 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2299 2803 4758 Size: 416 53 83 399 24 882 504 1955 Cuts at: 4758 5716 442 Size: 958 1753 Fragments arranged by size: 1955 1753 958 882 504 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7027 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 7027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2147 2600 3900 3945 4080 4292 5687 Size: 842 66 453 1300 45 135 212 1395 Cuts at: 5687 1239 Size: 2579 Fragments arranged by size: 2579 1395 1300 842 453 212 135 66 45 Bpu10I CC'TnA_GC Cuts at: 2130 2598 2651 2660 4164 4284 2130 Size: 468 53 9 1504 120 4873 Fragments arranged by size: 4873 1504 468 120 53 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3602 5152 6017 6315 3602 Size: 1550 865 298 4314 Fragments arranged by size: 4314 1550 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2860 4081 4310 5690 882 Size: 617 1361 1221 229 1380 2219 Fragments arranged by size: 2219 1380 1361 1221 617 229 BsaAI yAC'GTr Cuts at: 493 1204 4818 493 Size: 711 3614 2702 Fragments arranged by size: 3614 2702 711 BsaBI GATnn'nnATC Cuts at: 7021 7021 Size: 7027 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2976 3076 3441 4834 Size: 53 83 186 936 1443 100 365 1393 Cuts at: 4834 5216 5851 275 Size: 382 635 1451 Fragments arranged by size: 1451 1443 1393 936 635 382 365 186 100 83 53 BsaWI w'CCGG_w Cuts at: 2280 5460 5858 6132 6279 6690 2280 Size: 3180 398 274 147 411 2617 Fragments arranged by size: 3180 2617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2168 2198 2573 2603 1178 Size: 30 165 30 765 30 375 30 5602 Fragments arranged by size: 5602 765 375 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2155 2378 2474 2723 3254 3599 4116 1186 Size: 969 223 96 249 531 345 517 4097 Fragments arranged by size: 4097 969 531 517 345 249 223 96 BseYI C'CCAG_C Cuts at: 2850 3305 4084 4260 6230 6726 2850 Size: 455 779 176 1970 496 3151 Fragments arranged by size: 3151 1970 779 496 455 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4025 1163 Size: 573 2289 4165 Fragments arranged by size: 4165 2289 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2024 2097 4477 5238 6266 1540 Size: 275 186 23 73 2380 761 1028 2301 Fragments arranged by size: 2380 2301 1028 761 275 186 73 23 BsiHKAI G_wGCw'C Cuts at: 2433 3174 3779 3787 5088 5173 6244 2433 Size: 741 605 8 1301 85 1071 3216 Fragments arranged by size: 3216 1301 1071 741 605 85 8 BsmI GAATG_Cn' Cuts at: 4538 4631 4538 Size: 93 6934 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2433 2623 2823 3174 3383 3772 3779 Size: 399 643 190 200 351 209 389 7 Cuts at: 3779 3787 3893 3981 4236 4346 5088 5173 6244 Size: 8 106 88 255 110 742 85 1071 Cuts at: 6244 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 643 399 389 351 255 209 200 190 110 106 88 85 8 7 BspEI T'CCGG_A Cuts at: 6690 6690 Size: 7027 BspHI T'CATG_A Cuts at: 4916 6646 4916 Size: 1730 5297 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3091 4036 4382 844 Size: 1673 574 945 346 3489 Fragments arranged by size: 3489 1673 945 574 346 BsrBI CCG'CTC Cuts at: 1394 1823 2233 4216 4914 1394 Size: 429 410 1983 698 3507 Fragments arranged by size: 3507 1983 698 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 3867 5522 5696 65 Size: 1183 2619 1655 174 1396 Fragments arranged by size: 2619 1655 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 3504 5671 5858 1700 Size: 110 213 1481 2167 187 2869 Fragments arranged by size: 2869 2167 1481 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7027 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7027 BssSI C'ACGA_G Cuts at: 2881 3158 3365 5087 6099 2881 Size: 277 207 1722 1012 3809 Fragments arranged by size: 3809 1722 1012 277 207 BstAPI GCAn_nnn'nTGC Cuts at: 794 3418 3727 794 Size: 2624 309 4094 Fragments arranged by size: 4094 2624 309 BstBI TT'CG_AA Cuts at: 5831 5831 Size: 7027 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7027 BstXI CCAn_nnnn'nTGG Cuts at: 2746 3810 2746 Size: 1064 5963 BstYI r'GATC_y Cuts at: 1972 2422 2472 2646 2712 3361 3467 3483 4357 Size: 450 50 174 66 649 106 16 874 Cuts at: 4357 4724 5109 5126 6578 6664 6676 7022 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 874 649 450 385 367 346 174 106 86 66 50 17 16 12 Bsu36I CC'TnA_GG Cuts at: 2104 4228 2104 Size: 2124 4903 BtgI C'CryG_G Cuts at: 513 1065 2004 2097 2486 2585 3042 3384 3436 Size: 552 939 93 389 99 457 342 52 Cuts at: 3436 3624 3995 513 Size: 188 371 3545 Fragments arranged by size: 3545 939 552 457 389 371 342 188 99 93 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4194 487 Size: 683 56 2968 3320 Fragments arranged by size: 3320 2968 683 56 BtsI GCAGTG_nn' Cuts at: 2220 3315 3706 4543 5337 5357 2220 Size: 1095 391 837 794 20 3890 Fragments arranged by size: 3890 1095 837 794 391 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7027 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3910 3945 529 Size: 35 3346 35 3611 Fragments arranged by size: 3611 3346 35 35 DraI TTT'AAA Cuts at: 4331 4331 Size: 7027 DraIII CAC_nnn'GTG Cuts at: 2872 3727 2872 Size: 855 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 2852 6034 809 Size: 507 1284 252 3182 1802 Fragments arranged by size: 3182 1802 1284 507 252 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2021 2736 3034 Size: 54 1425 50 275 18 191 715 298 Cuts at: 3034 3621 3864 4239 4474 5363 6992 8 Size: 587 243 375 235 889 1629 43 Fragments arranged by size: 1629 1425 889 715 587 375 298 275 243 235 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2021 4474 1537 Size: 275 209 2453 4090 Fragments arranged by size: 4090 2453 275 209 EarI CTCTTCn'nnn_ Cuts at: 1353 2545 3073 4957 1353 Size: 1192 528 1884 3423 Fragments arranged by size: 3423 1884 1192 528 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2660 5614 5988 6134 387 Size: 1064 138 1071 2954 374 146 1280 Fragments arranged by size: 2954 1280 1071 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3785 3785 Size: 7027 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2147 2600 3821 3900 3945 4080 Size: 393 449 66 453 1221 79 45 135 Cuts at: 4080 4292 5090 5687 6474 6742 1239 Size: 212 798 597 787 268 1524 Fragments arranged by size: 1524 1221 798 787 597 453 449 393 268 212 135 79 66 45 EcoNI CCTnn'n_nnAGG Cuts at: 2912 4098 4227 2912 Size: 1186 129 5712 Fragments arranged by size: 5712 1186 129 EcoO109I rG'GnC_Cy Cuts at: 2250 2293 2620 3265 3769 3889 3890 4088 4121 Size: 43 327 645 504 120 1 198 33 Cuts at: 4121 2250 Size: 5156 Fragments arranged by size: 5156 645 504 327 198 120 43 33 1 EcoRI G'AATT_C Cuts at: 3655 4336 4714 3655 Size: 681 378 5968 Fragments arranged by size: 5968 681 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3355 3418 3355 Size: 63 6964 FspI TGC'GCA Cuts at: 2513 5533 2513 Size: 3020 4007 HaeII r_GCGC'y Cuts at: 1678 3444 3679 3949 4069 6174 1678 Size: 1766 235 270 120 2105 2531 Fragments arranged by size: 2531 2105 1766 270 235 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 5668 5700 5742 Size: 32 135 32 950 32 3114 32 42 Cuts at: 5742 5774 1373 Size: 32 2626 Fragments arranged by size: 3114 2626 950 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2530 4371 4617 669 Size: 988 281 238 354 1841 246 3079 Fragments arranged by size: 3079 1841 988 354 281 246 238 HindIII A'AGCT_T Cuts at: 748 748 Size: 7027 HpaI GTT'AAC Cuts at: 4617 4617 Size: 7027 KasI G'GCGC_C Cuts at: 3440 3440 Size: 7027 KpnI G_GTAC'C Cuts at: 4352 4352 Size: 7027 MfeI C'AATT_G Cuts at: 4626 4626 Size: 7027 MluI A'CGCG_T Cuts at: 6903 6903 Size: 7027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2571 6141 6325 1305 Size: 1266 3570 184 2007 Fragments arranged by size: 3570 2007 1266 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2738 3623 3866 4241 10 Size: 54 1425 343 906 885 243 375 2796 Fragments arranged by size: 2796 1425 906 885 375 343 243 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 2771 2796 3570 4985 5344 5503 Size: 648 1287 318 25 774 1415 359 159 Cuts at: 5503 518 Size: 2042 Fragments arranged by size: 2042 1415 1287 774 648 359 318 159 25 MspA1I CmG'CkG Cuts at: 1606 2020 2099 2246 2488 3286 3438 3670 3845 Size: 414 79 147 242 798 152 232 175 Cuts at: 3845 3886 3973 4168 4443 5120 6268 6513 1606 Size: 41 87 195 275 677 1148 245 2120 Fragments arranged by size: 2120 1148 798 677 414 275 245 242 232 195 175 152 147 87 79 41 NaeI GCC'GGC Cuts at: 2025 3506 2025 Size: 1481 5546 NarI GG'CG_CC Cuts at: 3441 3441 Size: 7027 NcoI C'CATG_G Cuts at: 513 1065 2004 3624 3995 513 Size: 552 939 1620 371 3545 Fragments arranged by size: 3545 1620 939 552 371 NdeI CA'TA_TG Cuts at: 387 387 Size: 7027 NgoMIV G'CCGG_C Cuts at: 2023 3504 2023 Size: 1481 5546 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7027 NotI GC'GGCC_GC Cuts at: 4474 4474 Size: 7027 NruI TCG'CGA Cuts at: 3237 3237 Size: 7027 NspI r_CATG'y Cuts at: 1132 1229 2932 2971 3018 3118 3283 3830 4385 Size: 97 1703 39 47 100 165 547 555 Cuts at: 4385 5930 1132 Size: 1545 2229 Fragments arranged by size: 2229 1703 1545 555 547 165 100 97 47 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7027 PciI A'CATG_T Cuts at: 3114 5926 3114 Size: 2812 4215 PflMI CCAn_nnn'nTGG Cuts at: 1108 3061 1108 Size: 1953 5074 PfoI T'CCnGG_A Cuts at: 4096 4096 Size: 7027 PmeI GTTT'AAAC Cuts at: 4331 4331 Size: 7027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5096 5128 6609 6641 5096 Size: 32 1481 32 5482 Fragments arranged by size: 5482 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 4088 4121 2293 Size: 1795 33 5199 Fragments arranged by size: 5199 1795 33 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7027 PsiI TTA'TAA Cuts at: 4597 4597 Size: 7027 PspOMI G'GGCC_C Cuts at: 2619 3768 3889 2619 Size: 1149 121 5757 Fragments arranged by size: 5757 1149 121 PstI C_TGCA'G Cuts at: 830 2811 2832 3322 3669 4379 830 Size: 1981 21 490 347 710 3478 Fragments arranged by size: 3478 1981 710 490 347 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7027 PvuII CAG'CTG Cuts at: 3845 3845 Size: 7027 SacI G_AGCT'C Cuts at: 3787 3787 Size: 7027 SacII CC_GC'GG Cuts at: 2100 2489 3439 2100 Size: 389 950 5688 Fragments arranged by size: 5688 950 389 SalI G'TCGA_C Cuts at: 1936 4369 1936 Size: 2433 4594 SanDI GG'GwC_CC Cuts at: 4088 4088 Size: 7027 SbfI CC_TGCA'GG Cuts at: 4379 4379 Size: 7027 ScaI AGT'ACT Cuts at: 3164 3807 5275 6987 3164 Size: 643 1468 1712 3204 Fragments arranged by size: 3204 1712 1468 643 SexAI A'CCwGG_T Cuts at: 2516 2516 Size: 7027 SfcI C'TryA_G Cuts at: 826 1045 2269 2807 2828 2961 3318 3665 4375 Size: 219 1224 538 21 133 357 347 710 Cuts at: 4375 5510 6191 6382 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 710 681 538 357 347 219 191 133 21 SfoI GGC'GCC Cuts at: 3442 3442 Size: 7027 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7027 SmaI CCC'GGG Cuts at: 1872 3446 4058 4301 4354 1872 Size: 1574 612 243 53 4545 Fragments arranged by size: 4545 1574 612 243 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 3581 5131 6032 6294 820 Size: 197 926 1638 1550 901 262 1553 Fragments arranged by size: 1638 1553 1550 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4818 493 Size: 4325 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7027 SphI G_CATG'C Cuts at: 1132 2971 4385 1132 Size: 1839 1414 3774 Fragments arranged by size: 3774 1839 1414 SspI AAT'ATT Cuts at: 5 52 4951 5 Size: 47 4899 2081 Fragments arranged by size: 4899 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7027 StyI C'CwwG_G Cuts at: 513 1065 2004 2149 2378 3624 3995 4250 4466 Size: 552 939 145 229 1246 371 255 216 Cuts at: 4466 513 Size: 3074 Fragments arranged by size: 3074 1246 939 552 371 255 229 216 145 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5054 5071 5224 5409 5854 325 Size: 1255 3474 17 153 185 445 1498 Fragments arranged by size: 3474 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3093 3111 3162 Size: 274 80 33 51 1443 1116 18 51 Cuts at: 3162 3399 3805 5273 6985 96 Size: 237 406 1468 1712 138 Fragments arranged by size: 1712 1468 1443 1116 406 274 237 138 80 51 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6987 7019 6987 Size: 32 6995 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7027 XbaI T'CTAG_A Cuts at: 4363 4363 Size: 7027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2866 2866 Size: 7027 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7027 XmaI C'CCGG_G Cuts at: 1870 3444 4056 4299 4352 1870 Size: 1574 612 243 53 4545 Fragments arranged by size: 4545 1574 612 243 53 XmnI GAAnn'nnTTC Cuts at: 5156 5156 Size: 7027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2977 4835 276 Size: 53 83 186 936 1443 1858 2468 Fragments arranged by size: 2468 1858 1443 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SrfI SwaI