Restriction map for
pFN21AE5724
(Linear) (Six-base) MAPSORT of: KIEE5724.seq Check: 1012 from: 1 to: 2322
KIEE5724.orf
With 182 enzymes: SgfI *
February 19, 2021 20:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1086 2031 2322
Size: 1086 945 291
Fragments arranged by size:
1086 945 291
AatII G_ACGT'C
Cuts at: 0 974 2322
Size: 974 1348
AccI GT'mk_AC
Cuts at: 0 599 2322
Size: 599 1723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1816 2322
Size: 1816 506
AfeI AGC'GCT
Cuts at: 0 1672 2322
Size: 1672 650
AflIII A'CryG_T
Cuts at: 0 1109 2322
Size: 1109 1213
AlwNI CAG_nnn'CTG
Cuts at: 0 810 1056 1843 2278 2322
Size: 810 246 787 435 44
Fragments arranged by size:
810 787 435 246 44
ApaI G_GGCC'C
Cuts at: 0 618 1767 1888 2322
Size: 618 1149 121 434
Fragments arranged by size:
1149 618 434 121
ApoI r'AATT_y
Cuts at: 0 1650 2322
Size: 1650 672
AvaI C'yCGr_G
Cuts at: 0 1439 1745 1767 2051 2120 2294 2322
Size: 1439 306 22 284 69 174 28
Fragments arranged by size:
1439 306 284 174 69 28 22
BamHI G'GATC_C
Cuts at: 0 1462 2322
Size: 1462 860
BanI G'GyrC_C
Cuts at: 0 63 140 477 813 951 1003 1435 1971
Size: 63 77 337 336 138 52 432 536
Cuts at: 1971 2322
Size: 351
Fragments arranged by size:
536 432 351 337 336 138 77 63
52
BanII G_rGCy'C
Cuts at: 0 618 1378 1767 1782 1888 2231 2322
Size: 618 760 389 15 106 343 91
Fragments arranged by size:
760 618 389 343 106 91 15
BbeI G_GCGC'C
Cuts at: 0 1439 2322
Size: 1439 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 1335 2322
Size: 375 960 987
Fragments arranged by size:
987 960 375
BbvCI CC'TCA_GC
Cuts at: 0 593 655 2159 2322
Size: 593 62 1504 163
Fragments arranged by size:
1504 593 163 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1170 1204 2322
Size: 1170 34 1118
Fragments arranged by size:
1170 1118 34
BclI T'GATC_A
Cuts at: 0 293 2322
Size: 293 2029
BglI GCCn_nnn'nGGC
Cuts at: 0 2265 2322
Size: 2265 57
BglII A'GATC_T
Cuts at: 0 707 1478 2322
Size: 707 771 844
Fragments arranged by size:
844 771 707
BlpI GC'TnA_GC
Cuts at: 0 988 1216 1781 1944 2322
Size: 988 228 565 163 378
Fragments arranged by size:
988 565 378 228 163
Bme1580I G_kGCm'C
Cuts at: 0 618 818 1767 1888 1976 2322
Size: 618 200 949 121 88 346
Fragments arranged by size:
949 618 346 200 121 88
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 798 2322
Size: 294 504 1524
Fragments arranged by size:
1524 504 294
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 2322
Size: 381 1941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 142 595 1895 1940 2075 2287 2322
Size: 76 66 453 1300 45 135 212 35
Fragments arranged by size:
1300 453 212 135 76 66 45 35
Bpu10I CC'TnA_GC
Cuts at: 0 125 593 646 655 2159 2279 2322
Size: 125 468 53 9 1504 120 43
Fragments arranged by size:
1504 468 125 120 53 43 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 2322
Size: 1597 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 855 2076 2305 2322
Size: 855 1221 229 17
Fragments arranged by size:
1221 855 229 17
BsaHI Gr'CG_yC
Cuts at: 0 971 1071 1436 2322
Size: 971 100 365 886
Fragments arranged by size:
971 886 365 100
BsaWI w'CCGG_w
Cuts at: 0 275 2322
Size: 275 2047
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 568 598 2322
Size: 163 30 375 30 1724
Fragments arranged by size:
1724 375 163 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 150 373 469 718 1249 1594 2111 2322
Size: 150 223 96 249 531 345 517 211
Fragments arranged by size:
531 517 345 249 223 211 150 96
BseYI C'CCAG_C
Cuts at: 0 845 1300 2079 2255 2322
Size: 845 455 779 176 67
Fragments arranged by size:
845 779 455 176 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2020 2322
Size: 2020 302
BsiEI CG_ry'CG
Cuts at: 0 19 92 2322
Size: 19 73 2230
Fragments arranged by size:
2230 73 19
BsiHKAI G_wGCw'C
Cuts at: 0 428 1169 1774 1782 2322
Size: 428 741 605 8 540
Fragments arranged by size:
741 605 540 428 8
Bsp1286I G_dGCh'C
Cuts at: 0 428 618 818 1169 1378 1767 1774 1782
Size: 428 190 200 351 209 389 7 8
Cuts at: 1782 1888 1976 2231 2322
Size: 106 88 255 91
Fragments arranged by size:
428 389 351 255 209 200 190 106
91 88 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 1086 2031 2322
Size: 512 574 945 291
Fragments arranged by size:
945 574 512 291
BsrBI CCG'CTC
Cuts at: 0 228 2211 2322
Size: 228 1983 111
Fragments arranged by size:
1983 228 111
BsrDI GCAATG_nn'
Cuts at: 0 1862 2322
Size: 1862 460
BsrFI r'CCGG_y
Cuts at: 0 18 1499 2322
Size: 18 1481 823
Fragments arranged by size:
1481 823 18
BssSI C'ACGA_G
Cuts at: 0 876 1153 1360 2322
Size: 876 277 207 962
Fragments arranged by size:
962 876 277 207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1413 1722 2322
Size: 1413 309 600
Fragments arranged by size:
1413 600 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 741 1805 2322
Size: 741 1064 517
Fragments arranged by size:
1064 741 517
BstYI r'GATC_y
Cuts at: 0 417 467 641 707 1356 1462 1478 2322
Size: 417 50 174 66 649 106 16 844
Fragments arranged by size:
844 649 417 174 106 66 50 16
Bsu36I CC'TnA_GG
Cuts at: 0 99 2223 2322
Size: 99 2124 99
Fragments arranged by size:
2124 99 99
BtgI C'CryG_G
Cuts at: 0 92 481 580 1037 1379 1431 1619 1990
Size: 92 389 99 457 342 52 188 371
Cuts at: 1990 2322
Size: 332
Fragments arranged by size:
457 389 371 342 332 188 99 92
52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2189 2322
Size: 2189 133
BtsI GCAGTG_nn'
Cuts at: 0 215 1310 1701 2322
Size: 215 1095 391 621
Fragments arranged by size:
1095 621 391 215
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1905 1940 2322
Size: 1905 35 382
Fragments arranged by size:
1905 382 35
DraIII CAC_nnn'GTG
Cuts at: 0 867 1722 2322
Size: 867 855 600
Fragments arranged by size:
867 855 600
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 847 2322
Size: 595 252 1475
Fragments arranged by size:
1475 595 252
EaeI y'GGCC_r
Cuts at: 0 16 731 1029 1616 1859 2234 2322
Size: 16 715 298 587 243 375 88
Fragments arranged by size:
715 587 375 298 243 88 16
EagI C'GGCC_G
Cuts at: 0 16 2322
Size: 16 2306
EarI CTCTTCn'nnn_
Cuts at: 0 540 1068 2322
Size: 540 528 1254
Fragments arranged by size:
1254 540 528
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 655 2322
Size: 655 1667
EcoICRI GAG'CTC
Cuts at: 0 1780 2322
Size: 1780 542
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 142 595 1816 1895 1940 2075 2287
Size: 76 66 453 1221 79 45 135 212
Cuts at: 2287 2322
Size: 35
Fragments arranged by size:
1221 453 212 135 79 76 66 45
35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 907 2093 2222 2322
Size: 907 1186 129 100
Fragments arranged by size:
1186 907 129 100
EcoO109I rG'GnC_Cy
Cuts at: 0 245 288 615 1260 1764 1884 1885 2083
Size: 245 43 327 645 504 120 1 198
Cuts at: 2083 2116 2322
Size: 33 206
Fragments arranged by size:
645 504 327 245 206 198 120 43
33 1
EcoRI G'AATT_C
Cuts at: 0 1650 2322
Size: 1650 672
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1350 1413 2322
Size: 1350 63 909
Fragments arranged by size:
1350 909 63
FspI TGC'GCA
Cuts at: 0 508 2322
Size: 508 1814
HaeII r_GCGC'y
Cuts at: 0 1439 1674 1944 2064 2322
Size: 1439 235 270 120 258
Fragments arranged by size:
1439 270 258 235 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 2322
Size: 517 32 1773
Fragments arranged by size:
1773 517 32
HincII GTy'rAC
Cuts at: 0 171 525 2322
Size: 171 354 1797
Fragments arranged by size:
1797 354 171
KasI G'GCGC_C
Cuts at: 0 1435 2322
Size: 1435 887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 2322
Size: 566 1756
MscI TGG'CCA
Cuts at: 0 733 1618 1861 2236 2322
Size: 733 885 243 375 86
Fragments arranged by size:
885 733 375 243 86
MslI CAynn'nnrTG
Cuts at: 0 448 766 791 1565 2322
Size: 448 318 25 774 757
Fragments arranged by size:
774 757 448 318 25
MspA1I CmG'CkG
Cuts at: 0 15 94 241 483 1281 1433 1665 1840
Size: 15 79 147 242 798 152 232 175
Cuts at: 1840 1881 1968 2163 2322
Size: 41 87 195 159
Fragments arranged by size:
798 242 232 195 175 159 152 147
87 79 41 15
NaeI GCC'GGC
Cuts at: 0 20 1501 2322
Size: 20 1481 821
Fragments arranged by size:
1481 821 20
NarI GG'CG_CC
Cuts at: 0 1436 2322
Size: 1436 886
NcoI C'CATG_G
Cuts at: 0 1619 1990 2322
Size: 1619 371 332
Fragments arranged by size:
1619 371 332
NgoMIV G'CCGG_C
Cuts at: 0 18 1499 2322
Size: 18 1481 823
Fragments arranged by size:
1481 823 18
NruI TCG'CGA
Cuts at: 0 1232 2322
Size: 1232 1090
NspI r_CATG'y
Cuts at: 0 927 966 1013 1113 1278 1825 2322
Size: 927 39 47 100 165 547 497
Fragments arranged by size:
927 547 497 165 100 47 39
PciI A'CATG_T
Cuts at: 0 1109 2322
Size: 1109 1213
PflMI CCAn_nnn'nTGG
Cuts at: 0 1056 2322
Size: 1056 1266
PfoI T'CCnGG_A
Cuts at: 0 2091 2322
Size: 2091 231
PpuMI rG'GwC_Cy
Cuts at: 0 288 2083 2116 2322
Size: 288 1795 33 206
Fragments arranged by size:
1795 288 206 33
PspOMI G'GGCC_C
Cuts at: 0 614 1763 1884 2322
Size: 614 1149 121 438
Fragments arranged by size:
1149 614 438 121
PstI C_TGCA'G
Cuts at: 0 806 827 1317 1664 2322
Size: 806 21 490 347 658
Fragments arranged by size:
806 658 490 347 21
PvuII CAG'CTG
Cuts at: 0 1840 2322
Size: 1840 482
SacI G_AGCT'C
Cuts at: 0 1782 2322
Size: 1782 540
SacII CC_GC'GG
Cuts at: 0 95 484 1434 2322
Size: 95 389 950 888
Fragments arranged by size:
950 888 389 95
SanDI GG'GwC_CC
Cuts at: 0 2083 2322
Size: 2083 239
ScaI AGT'ACT
Cuts at: 0 1159 1802 2322
Size: 1159 643 520
Fragments arranged by size:
1159 643 520
SexAI A'CCwGG_T
Cuts at: 0 511 2322
Size: 511 1811
SfcI C'TryA_G
Cuts at: 0 264 802 823 956 1313 1660 2322
Size: 264 538 21 133 357 347 662
Fragments arranged by size:
662 538 357 347 264 133 21
SfoI GGC'GCC
Cuts at: 0 1437 2322
Size: 1437 885
SmaI CCC'GGG
Cuts at: 0 1441 2053 2296 2322
Size: 1441 612 243 26
Fragments arranged by size:
1441 612 243 26
SmlI C'TyrA_G
Cuts at: 0 1576 2322
Size: 1576 746
SphI G_CATG'C
Cuts at: 0 966 2322
Size: 966 1356
StyI C'CwwG_G
Cuts at: 0 144 373 1619 1990 2245 2322
Size: 144 229 1246 371 255 77
Fragments arranged by size:
1246 371 255 229 144 77
TatI w'GTAC_w
Cuts at: 0 1088 1106 1157 1394 1800 2322
Size: 1088 18 51 237 406 522
Fragments arranged by size:
1088 522 406 237 51 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 2322
Size: 861 1461
XmaI C'CCGG_G
Cuts at: 0 1439 2051 2294 2322
Size: 1439 612 243 28
Fragments arranged by size:
1439 612 243 28
ZraI GAC'GTC
Cuts at: 0 972 2322
Size: 972 1350
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PciI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SacII SanDI ScaI
SexAI SfcI SfoI SmaI SmlI SphI StyI TatI
XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BciVI BfrBI
BmgBI BmtI BsaAI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmnI