################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BcgI BfrI BlnI BsaAI BsaBI BsaHI BsiWI BspDI BspLU11I Bst1107I BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 283 GTCGAC GT^[AC][GT]AC AocI 49 CCTCAGG CC^T[ACGT]AGG BamHI 535 GGATCC G^GATCC BanI 2283 GGCACC G^G[CT][GA]CC BbsI 1312 GTCTTC GAAGAC(2/6) BbvCI 1254 CCTCAGC CCTCAGC(-5/-2) BciVI 551 GGATAC GTATCC(6/5) BclI 2234 TGATCA T^GATCA BfaI 2182 CTAG C^TAG BglII 1781 AGATCT A^GATCT BpuAI 1312 GTCTTC GAAGAC(2/6) BsaI 2125 GAGACC GGTCTC(1/5) BsgI 2518 CTGCAC GTGCAG(16/14) BsiEI 2359 CGGCCG CG[GA][CT]^CG Bsp1407I 776 TGTACA T^GTACA BsrGI 776 TGTACA T^GTACA BssHII 1000 GCGCGC G^CGCGC BstAPI 851 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 49 CCTCAGG CC^T[ACGT]AGG DraIII 1212 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 89 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2359 CGGCCG C^GGCCG EclXI 2359 CGGCCG C^GGCCG Eco52I 2359 CGGCCG C^GGCCG Eco81I 49 CCTCAGG CC^T[ACGT]AGG EcoRI 589 GAATTC G^AATTC EcoRV 2383 GATATC GAT^ATC FbaI 2234 TGATCA T^GATCA HindIII 1865 AAGCTT A^AGCTT MaeI 2182 CTAG C^TAG MfeI 1160 CAATTG C^AATTG MunI 1160 CAATTG C^AATTG NcoI 2647 CCATGG C^CATGG NspI 254 GCATGC [GA]CATG^[CT] SalI 283 GTCGAC G^TCGAC SmaI 116 CCCGGG CCC^GGG SphI 254 GCATGC GCATG^C SspBI 776 TGTACA T^GTACA XmaCI 116 CCCGGG C^CCGGG XmaI 116 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccIII 548 TCCGGA T^CCGGA 2217 TCCGGA AcsI 469 GAATTT [GA]^AATT[CT] 589 GAATTC Alw44I 322 GTGCAC G^TGCAC 832 GTGCAC AlwNI 568 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 679 CAGAAGCTG ApaLI 322 GTGCAC G^TGCAC 832 GTGCAC ApoI 469 GAATTT [GA]^AATT[CT] 589 GAATTC Asp700I 1168 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1203 GAATGCCTTC BseAI 548 TCCGGA T^CCGGA 2217 TCCGGA BsmBI 363 CGTCTC CGTCTC(1/5) 2355 GAGACG BsmI 1203 GAATGC GAATGC(1/-1) 2021 GCATTC BspEI 548 TCCGGA T^CCGGA 2217 TCCGGA BspHI 933 TCATGA T^CATGA 1619 TCATGA BsrBI 427 CCGCTC CCGCTC(-3/-3) 1682 CCGCTC BsrDI 651 CATTGC GCAATG(2/0) 2451 CATTGC BsrFI 38 GCCGGC [GA]^CCGG[CT] 2877 GCCGGC BstEII 1588 GGTTACC G^GT[ACGT]ACC 1972 GGTTACC BstPI 1588 GGTTACC G^GT[ACGT]ACC 1972 GGTTACC BstXI 1346 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2167 CCAGAGCAGTGG Cfr10I 38 GCCGGC [GA]^CCGG[CT] 2877 GCCGGC EcoO65I 1588 GGTTACC G^GT[ACGT]ACC 1972 GGTTACC HaeII 1789 GGCGCT [GA]GCGC^[CT] 1850 AGCGCC HgaI 2577 GACGC GACGC(5/10) 2750 GCGTC HincII 283 GTCGAC GT[CT]^[GA]AC 2530 GTCAAC HindII 283 GTCGAC GT[CT]^[GA]AC 2530 GTCAAC MroI 548 TCCGGA T^CCGGA 2217 TCCGGA MseI 887 TTAA T^TAA 2627 TTAA NaeI 38 GCCGGC GCC^GGC 2877 GCCGGC NgoMIV 38 GCCGGC G^CCGGC 2877 GCCGGC PvuII 2563 CAGCTG CAG^CTG 2953 CAGCTG RcaI 933 TCATGA T^CATGA 1619 TCATGA SacI 687 GAGCTC GAGCT^C 2014 GAGCTC SapI 1846 GAAGAGC GCTCTTC(1/4) 2814 GAAGAGC ScaI 2492 AGTACT AGT^ACT 2681 AGTACT StuI 1447 AGGCCT AGG^CCT 1942 AGGCCT TfiI 2390 GAATC G^A[AT]TC 2769 GATTC Tru9I 887 TTAA T^TAA 2627 TTAA XmnI 1168 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1203 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AfaI AluI AlwI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstBI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII