(Circular) (Six-base) MAPSORT of: pFN21AE5805.seq Check: 3384 from: 1 to: 7702 pFN21AE5805 7702 bp With 182 enzymes: SgfI * July 25, 2014 13:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2563 2709 2563 Size: 146 7556 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5512 278 Size: 53 83 186 936 3976 2468 Fragments arranged by size: 3976 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2289 5045 1937 Size: 352 2756 4594 Fragments arranged by size: 4594 2756 352 Acc65I G'GTAC_C Cuts at: 5023 5023 Size: 7702 AclI AA'CG_TT Cuts at: 1563 5829 6202 1563 Size: 4266 373 3063 Fragments arranged by size: 4266 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2870 4592 4929 5765 7149 7417 1632 Size: 399 839 1722 337 836 1384 268 1917 Fragments arranged by size: 1917 1722 1384 839 836 399 337 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7505 AflIII A'CryG_T Cuts at: 1203 6601 7578 1203 Size: 5398 977 1327 Fragments arranged by size: 5398 1327 977 AgeI A'CCGG_T Cuts at: 6533 6533 Size: 7702 AhdI GACnn_n'nnGTC Cuts at: 6431 6431 Size: 7702 AleI CACnn'nnGTG Cuts at: 1166 4997 1166 Size: 3831 3871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7702 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3596 3628 4604 4636 3596 Size: 32 976 32 6662 Fragments arranged by size: 6662 976 32 32 AlwNI CAG_nnn'CTG Cuts at: 2578 2689 5445 7017 2578 Size: 111 2756 1572 3263 Fragments arranged by size: 3263 2756 1572 111 ApaI G_GGCC'C Cuts at: 2084 2128 2411 4660 2084 Size: 44 283 2249 5126 Fragments arranged by size: 5126 2249 283 44 ApaLI G'TGCA_C Cuts at: 2327 2837 5759 6915 2327 Size: 510 2922 1156 3114 Fragments arranged by size: 3114 2922 1156 510 ApoI r'AATT_y Cuts at: 1493 2474 2594 5011 5237 5389 7498 1493 Size: 981 120 2417 226 152 2109 1697 Fragments arranged by size: 2417 2109 1697 981 226 152 120 AseI AT'TA_AT Cuts at: 160 1032 6256 160 Size: 872 5224 1606 Fragments arranged by size: 5224 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7702 AvaI C'yCGr_G Cuts at: 1870 1943 2121 2709 2868 5027 1870 Size: 73 178 588 159 2159 4545 Fragments arranged by size: 4545 2159 588 178 159 73 BamHI G'GATC_C Cuts at: 2540 5032 5399 2540 Size: 2492 367 4843 Fragments arranged by size: 4843 2492 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4288 5023 6478 618 Size: 325 217 627 2501 735 1455 1842 Fragments arranged by size: 2501 1842 1455 735 627 325 217 BanII G_rGCy'C Cuts at: 1391 2054 2084 2128 2411 2696 4023 4660 4898 Size: 663 30 44 283 285 1327 637 238 Cuts at: 4898 5021 1391 Size: 123 4072 Fragments arranged by size: 4072 1327 663 637 285 283 238 123 44 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3310 928 Size: 971 1411 5320 Fragments arranged by size: 5320 1411 971 BbvCI CC'TCA_GC Cuts at: 3260 3260 Size: 7702 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5893 5927 1903 Size: 34 50 34 3872 34 3678 Fragments arranged by size: 3872 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2550 5594 6804 2550 Size: 3044 1210 3448 Fragments arranged by size: 3448 3044 1210 BclI T'GATC_A Cuts at: 1548 4239 1548 Size: 2691 5011 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4712 4962 Size: 107 122 71 631 605 916 2124 250 Cuts at: 4962 6313 136 Size: 1351 1525 Fragments arranged by size: 2124 1525 1351 916 631 605 250 122 107 71 BglII A'GATC_T Cuts at: 3786 7697 3786 Size: 3911 3791 BlpI GC'TnA_GC Cuts at: 2048 2690 3265 4034 4936 5119 2048 Size: 642 575 769 902 183 4631 Fragments arranged by size: 4631 902 769 642 575 183 Bme1580I G_kGCm'C Cuts at: 1790 2084 2128 2331 2411 2841 3439 4660 5763 Size: 294 44 203 80 430 598 1221 1103 Cuts at: 5763 6919 1790 Size: 1156 2573 Fragments arranged by size: 2573 1221 1156 1103 598 430 294 203 80 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3398 5433 6391 Size: 416 53 83 399 24 1981 2035 958 Cuts at: 6391 442 Size: 1753 Fragments arranged by size: 2035 1981 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7702 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2525 2746 3984 4161 6362 1239 Size: 1226 60 221 1238 177 2201 2579 Fragments arranged by size: 2579 2201 1238 1226 221 177 60 Bpu10I CC'TnA_GC Cuts at: 3260 4751 3260 Size: 1491 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 2531 3866 4850 5827 6692 6990 2090 Size: 441 1335 984 977 865 298 2802 Fragments arranged by size: 2802 1335 984 977 865 441 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4124 6365 882 Size: 617 2625 2241 2219 Fragments arranged by size: 2625 2241 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5493 493 Size: 711 4289 2702 Fragments arranged by size: 4289 2702 711 BsaBI GATnn'nnATC Cuts at: 7696 7696 Size: 7702 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5509 5891 6526 275 Size: 53 83 186 936 3976 382 635 1451 Fragments arranged by size: 3976 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2313 2553 4222 6135 6533 6807 6954 7365 2313 Size: 240 1669 1913 398 274 147 411 2650 Fragments arranged by size: 2650 1913 1669 411 398 274 240 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 2744 2759 2774 Size: 30 165 30 607 30 704 15 15 Cuts at: 2774 2789 3126 3156 3596 3626 4484 4514 4532 Size: 15 337 30 440 30 858 30 18 Cuts at: 4532 4562 4604 4634 1178 Size: 30 42 30 4246 Fragments arranged by size: 4246 858 704 607 440 337 165 42 30 30 30 30 30 30 30 30 18 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 2465 4295 4865 4906 1186 Size: 1276 3 1830 570 41 3982 Fragments arranged by size: 3982 1830 1276 570 41 3 BseYI C'CCAG_C Cuts at: 2402 2425 3091 3182 4960 6905 7401 2402 Size: 23 666 91 1778 1945 496 2703 Fragments arranged by size: 2703 1945 1778 666 496 91 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4508 1163 Size: 573 2772 4357 Fragments arranged by size: 4357 2772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4367 5152 5913 6941 1540 Size: 275 186 2366 785 761 1028 2301 Fragments arranged by size: 2366 2301 1028 785 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2331 2484 2696 2841 4000 4023 5763 5848 Size: 145 153 212 145 1159 23 1740 85 Cuts at: 5848 6919 2186 Size: 1071 2969 Fragments arranged by size: 2969 1740 1159 1071 212 153 145 145 85 23 BsmI GAATG_Cn' Cuts at: 3214 4026 5213 5306 3214 Size: 812 1187 93 5610 Fragments arranged by size: 5610 1187 812 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2374 4354 2374 Size: 1980 5722 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2084 2128 2186 2331 2411 2484 Size: 399 264 30 44 58 145 80 73 Cuts at: 2484 2696 2841 3439 4000 4023 4660 4898 5021 Size: 212 145 598 561 23 637 238 123 Cuts at: 5021 5763 5848 6919 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 637 598 561 399 264 238 212 145 145 123 85 80 73 58 44 30 23 BspEI T'CCGG_A Cuts at: 2553 4222 7365 2553 Size: 1669 3143 2890 Fragments arranged by size: 3143 2890 1669 BspHI T'CATG_A Cuts at: 2938 3624 5591 7321 2938 Size: 686 1967 1730 3319 Fragments arranged by size: 3319 1967 1730 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2563 2709 4617 5057 844 Size: 1719 146 1908 440 3489 Fragments arranged by size: 3489 1908 1719 440 146 BsrBI CCG'CTC Cuts at: 1394 1823 2434 3689 5589 1394 Size: 429 611 1255 1900 3507 Fragments arranged by size: 3507 1900 1255 611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2655 4455 6197 6371 65 Size: 1183 1407 1800 1742 174 1396 Fragments arranged by size: 1800 1742 1407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 4882 6346 6533 1700 Size: 110 233 2839 1464 187 2869 Fragments arranged by size: 2869 2839 1464 233 187 110 BsrGI T'GTAC_A Cuts at: 96 2781 96 Size: 2685 5017 BssHII G'CGCG_C Cuts at: 1925 3005 1925 Size: 1080 6622 BssSI C'ACGA_G Cuts at: 2840 3761 4739 4760 5762 6774 2840 Size: 921 978 21 1002 1012 3768 Fragments arranged by size: 3768 1012 1002 978 921 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 2862 794 Size: 2068 5634 BstBI TT'CG_AA Cuts at: 4114 4163 4679 6506 4114 Size: 49 516 1827 5310 Fragments arranged by size: 5310 1827 516 49 BstEII G'GTnAC_C Cuts at: 1184 3593 3977 1184 Size: 2409 384 4909 Fragments arranged by size: 4909 2409 384 BstXI CCAn_nnnn'nTGG Cuts at: 3358 4179 3358 Size: 821 6881 BstYI r'GATC_y Cuts at: 1972 2155 2540 2844 3786 5032 5399 5784 5801 Size: 183 385 304 942 1246 367 385 17 Cuts at: 5801 7253 7339 7351 7697 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1246 942 385 385 367 346 304 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2055 2055 Size: 7702 BtgI C'CryG_G Cuts at: 513 1065 2567 4652 4788 513 Size: 552 1502 2085 136 3427 Fragments arranged by size: 3427 2085 1502 552 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3561 487 Size: 683 56 2335 4628 Fragments arranged by size: 4628 2335 683 56 BtsI GCAGTG_nn' Cuts at: 2105 4184 5218 6012 6032 2105 Size: 2079 1034 794 20 3775 Fragments arranged by size: 3775 2079 1034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7702 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7667 DraI TTT'AAA Cuts at: 5006 5006 Size: 7702 DraIII CAC_nnn'GTG Cuts at: 3222 3222 Size: 7702 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2100 6709 809 Size: 507 784 4609 1802 Fragments arranged by size: 4609 1802 784 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2161 2350 2580 Size: 54 1425 50 275 18 331 189 230 Cuts at: 2580 3801 4364 5149 6038 7667 8 Size: 1221 563 785 889 1629 43 Fragments arranged by size: 1629 1425 1221 889 785 563 331 275 230 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4364 5149 1537 Size: 275 2552 785 4090 Fragments arranged by size: 4090 2552 785 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 3365 3414 3846 3984 4271 4814 5632 Size: 1587 425 49 432 138 287 543 818 Cuts at: 5632 1353 Size: 3423 Fragments arranged by size: 3423 1587 818 543 432 425 287 138 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6289 6663 6809 387 Size: 1064 138 4700 374 146 1280 Fragments arranged by size: 4700 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2694 4021 2694 Size: 1327 6375 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2465 2525 2746 2870 3984 4161 Size: 393 399 434 60 221 124 1114 177 Cuts at: 4161 4592 4929 5765 6362 7149 7417 1239 Size: 431 337 836 597 787 268 1524 Fragments arranged by size: 1524 1114 836 787 597 434 431 399 393 337 268 221 177 124 60 EcoNI CCTnn'n_nnAGG Cuts at: 2058 2447 4847 2058 Size: 389 2400 4913 Fragments arranged by size: 4913 2400 389 EcoO109I rG'GnC_Cy Cuts at: 2080 2081 2117 2125 2407 3403 4183 4657 2080 Size: 1 36 8 282 996 780 474 5125 Fragments arranged by size: 5125 996 780 474 282 36 8 1 EcoRI G'AATT_C Cuts at: 2594 5011 5389 2594 Size: 2417 378 4907 Fragments arranged by size: 4907 2417 378 EcoRV GAT'ATC Cuts at: 4390 4390 Size: 7702 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2821 2821 Size: 7702 FspI TGC'GCA Cuts at: 6208 6208 Size: 7702 HaeII r_GCGC'y Cuts at: 1678 3798 3859 6849 1678 Size: 2120 61 2990 2531 Fragments arranged by size: 2990 2531 2120 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 2219 2251 3652 Size: 32 135 32 529 32 86 32 1401 Cuts at: 3652 3684 4397 4429 6343 6375 6417 6449 1373 Size: 32 713 32 1914 32 42 32 2626 Fragments arranged by size: 2626 1914 1401 713 529 135 86 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2290 4537 5046 5292 669 Size: 988 281 352 2247 509 246 3079 Fragments arranged by size: 3079 2247 988 509 352 281 246 HindIII A'AGCT_T Cuts at: 748 3870 748 Size: 3122 4580 HpaI GTT'AAC Cuts at: 5292 5292 Size: 7702 KpnI G_GTAC'C Cuts at: 5027 5027 Size: 7702 MfeI C'AATT_G Cuts at: 3165 5301 3165 Size: 2136 5566 MluI A'CGCG_T Cuts at: 7578 7578 Size: 7702 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2172 3055 4494 6816 7000 1305 Size: 867 883 1439 2322 184 2007 Fragments arranged by size: 2322 2007 1439 883 867 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2163 2352 2582 3803 10 Size: 54 1425 343 331 189 230 1221 3909 Fragments arranged by size: 3909 1425 1221 343 331 230 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2268 2943 4043 4235 4322 4995 4997 Size: 648 1102 675 1100 192 87 673 2 Cuts at: 4997 5660 6019 6178 518 Size: 663 359 159 2042 Fragments arranged by size: 2042 1102 1100 675 673 663 648 359 192 159 87 2 MspA1I CmG'CkG Cuts at: 1606 2467 4570 4960 5118 5795 6943 7188 1606 Size: 861 2103 390 158 677 1148 245 2120 Fragments arranged by size: 2120 2103 1148 861 677 390 245 158 NaeI GCC'GGC Cuts at: 2045 4884 2045 Size: 2839 4863 NcoI C'CATG_G Cuts at: 513 1065 4652 513 Size: 552 3587 3563 Fragments arranged by size: 3587 3563 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7702 NgoMIV G'CCGG_C Cuts at: 2043 4882 2043 Size: 2839 4863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7702 NotI GC'GGCC_GC Cuts at: 5149 5149 Size: 7702 NspI r_CATG'y Cuts at: 1132 1229 2263 5060 6605 1132 Size: 97 1034 2797 1545 2229 Fragments arranged by size: 2797 2229 1545 1034 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7702 PciI A'CATG_T Cuts at: 6601 6601 Size: 7702 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 3520 4904 1108 Size: 1251 1161 1384 3906 Fragments arranged by size: 3906 1384 1251 1161 PfoI T'CCnGG_A Cuts at: 2790 3357 3777 2790 Size: 567 420 6715 Fragments arranged by size: 6715 567 420 PmeI GTTT'AAAC Cuts at: 5006 5006 Size: 7702 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3248 3280 3596 3628 4604 4636 5771 5803 7284 Size: 32 316 32 976 32 1135 32 1481 Cuts at: 7284 7316 3248 Size: 32 3634 Fragments arranged by size: 3634 1481 1135 976 316 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2117 3403 4183 2117 Size: 1286 780 5636 Fragments arranged by size: 5636 1286 780 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7702 PsiI TTA'TAA Cuts at: 5272 5272 Size: 7702 PspOMI G'GGCC_C Cuts at: 2080 2124 2407 4656 2080 Size: 44 283 2249 5126 Fragments arranged by size: 5126 2249 283 44 PstI C_TGCA'G Cuts at: 830 2139 2685 2706 3685 4628 5054 830 Size: 1309 546 21 979 943 426 3478 Fragments arranged by size: 3478 1309 979 943 546 426 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7702 PvuII CAG'CTG Cuts at: 4570 4960 4570 Size: 390 7312 SacI G_AGCT'C Cuts at: 2696 4023 2696 Size: 1327 6375 SalI G'TCGA_C Cuts at: 1936 2288 5044 1936 Size: 352 2756 4594 Fragments arranged by size: 4594 2756 352 SapI GCTCTTCn'nnn_ Cuts at: 3846 4814 3846 Size: 968 6734 SbfI CC_TGCA'GG Cuts at: 5054 5054 Size: 7702 ScaI AGT'ACT Cuts at: 4499 4688 5950 7662 4499 Size: 189 1262 1712 4539 Fragments arranged by size: 4539 1712 1262 189 SfcI C'TryA_G Cuts at: 826 1045 2135 2681 2702 3377 3681 4192 4504 Size: 219 1090 546 21 675 304 511 312 Cuts at: 4504 4624 5050 6185 6866 7057 826 Size: 120 426 1135 681 191 1471 Fragments arranged by size: 1471 1135 1090 681 675 546 511 426 312 304 219 191 120 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7702 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7702 SmaI CCC'GGG Cuts at: 1872 2123 5029 1872 Size: 251 2906 4545 Fragments arranged by size: 4545 2906 251 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2510 3881 4865 5806 6707 Size: 197 926 162 405 1371 984 941 901 Cuts at: 6707 6969 820 Size: 262 1553 Fragments arranged by size: 1553 1371 984 941 926 901 405 262 197 162 SnaBI TAC'GTA Cuts at: 493 5493 493 Size: 5000 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7702 SphI G_CATG'C Cuts at: 1132 2263 5060 1132 Size: 1131 2797 3774 Fragments arranged by size: 3774 2797 1131 SspI AAT'ATT Cuts at: 5 52 5626 5 Size: 47 5574 2081 Fragments arranged by size: 5574 2081 47 StuI AGG'CCT Cuts at: 1517 3454 3949 1517 Size: 1937 495 5270 Fragments arranged by size: 5270 1937 495 StyI C'CwwG_G Cuts at: 513 1065 2238 2338 4652 5141 513 Size: 552 1173 100 2314 489 3074 Fragments arranged by size: 3074 2314 1173 552 489 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4148 5729 5746 5899 6084 6529 325 Size: 1255 2568 1581 17 153 185 445 1498 Fragments arranged by size: 2568 1581 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2296 2781 4227 Size: 274 80 33 51 1443 319 485 1446 Cuts at: 4227 4497 4686 5948 7660 96 Size: 270 189 1262 1712 138 Fragments arranged by size: 1712 1446 1443 1262 485 319 274 270 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3126 3158 3208 3240 4532 4564 7662 7694 3126 Size: 32 50 32 1292 32 3098 32 3134 Fragments arranged by size: 3134 3098 1292 50 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7702 XbaI T'CTAG_A Cuts at: 5038 5038 Size: 7702 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2171 2345 2881 3913 2171 Size: 174 536 1032 5960 Fragments arranged by size: 5960 1032 536 174 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7702 XmaI C'CCGG_G Cuts at: 1870 2121 5027 1870 Size: 251 2906 4545 Fragments arranged by size: 4545 2906 251 XmnI GAAnn'nnTTC Cuts at: 3177 3212 5831 3177 Size: 35 2619 5048 Fragments arranged by size: 5048 2619 35 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5510 276 Size: 53 83 186 936 3976 2468 Fragments arranged by size: 3976 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI