(Linear) (Six-base) MAPSORT of: KIEE5805.seq Check: 3176 from: 1 to: 2997 KIEE5805.orf With 182 enzymes: SgfI * July 25, 2014 11:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 558 704 2997 Size: 558 146 2293 Fragments arranged by size: 2293 558 146 AccI GT'mk_AC Cuts at: 0 284 2997 Size: 284 2713 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 865 2587 2924 2997 Size: 26 839 1722 337 73 Fragments arranged by size: 1722 839 337 73 26 AleI CACnn'nnGTG Cuts at: 0 2992 2997 Size: 2992 5 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1591 1623 2599 2631 2997 Size: 1591 32 976 32 366 Fragments arranged by size: 1591 976 366 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 573 684 2997 Size: 573 111 2313 Fragments arranged by size: 2313 573 111 ApaI G_GGCC'C Cuts at: 0 79 123 406 2655 2997 Size: 79 44 283 2249 342 Fragments arranged by size: 2249 342 283 79 44 ApaLI G'TGCA_C Cuts at: 0 322 832 2997 Size: 322 510 2165 Fragments arranged by size: 2165 510 322 ApoI r'AATT_y Cuts at: 0 469 589 2997 Size: 469 120 2408 Fragments arranged by size: 2408 469 120 AvaI C'yCGr_G Cuts at: 0 116 704 863 2997 Size: 116 588 159 2134 Fragments arranged by size: 2134 588 159 116 BamHI G'GATC_C Cuts at: 0 535 2997 Size: 535 2462 BanI G'GyrC_C Cuts at: 0 2283 2997 Size: 2283 714 BanII G_rGCy'C Cuts at: 0 49 79 123 406 691 2018 2655 2893 Size: 49 30 44 283 285 1327 637 238 Cuts at: 2893 2997 Size: 104 Fragments arranged by size: 1327 637 285 283 238 104 49 44 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 1305 2997 Size: 1305 1692 BbvCI CC'TCA_GC Cuts at: 0 1255 2997 Size: 1255 1742 BciVI GTATCCnnnnn_n' Cuts at: 0 545 2997 Size: 545 2452 BclI T'GATC_A Cuts at: 0 2234 2997 Size: 2234 763 BglI GCCn_nnn'nGGC Cuts at: 0 583 2707 2957 2997 Size: 583 2124 250 40 Fragments arranged by size: 2124 583 250 40 BglII A'GATC_T Cuts at: 0 1781 2997 Size: 1781 1216 BlpI GC'TnA_GC Cuts at: 0 43 685 1260 2029 2931 2997 Size: 43 642 575 769 902 66 Fragments arranged by size: 902 769 642 575 66 43 Bme1580I G_kGCm'C Cuts at: 0 79 123 326 406 836 1434 2655 2997 Size: 79 44 203 80 430 598 1221 342 Fragments arranged by size: 1221 598 430 342 203 80 79 44 BmrI ACTGGGnnnn_n' Cuts at: 0 1393 2997 Size: 1393 1604 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 520 741 1979 2156 2997 Size: 460 60 221 1238 177 841 Fragments arranged by size: 1238 841 460 221 177 60 Bpu10I CC'TnA_GC Cuts at: 0 1255 2746 2997 Size: 1255 1491 251 Fragments arranged by size: 1491 1255 251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 526 1861 2845 2997 Size: 85 441 1335 984 152 Fragments arranged by size: 1335 984 441 152 85 BsaI GGTCTCn'nnnn_ Cuts at: 0 2119 2997 Size: 2119 878 BsaWI w'CCGG_w Cuts at: 0 308 548 2217 2997 Size: 308 240 1669 780 Fragments arranged by size: 1669 780 308 240 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 5 35 739 754 769 784 1121 1151 Size: 5 30 704 15 15 15 337 30 Cuts at: 1151 1591 1621 2479 2509 2527 2557 2599 2629 Size: 440 30 858 30 18 30 42 30 Cuts at: 2629 2997 Size: 368 Fragments arranged by size: 858 704 440 368 337 42 30 30 30 30 30 30 18 15 15 15 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 457 460 2290 2860 2901 2997 Size: 457 3 1830 570 41 96 Fragments arranged by size: 1830 570 457 96 41 3 BseYI C'CCAG_C Cuts at: 0 397 420 1086 1177 2955 2997 Size: 397 23 666 91 1778 42 Fragments arranged by size: 1778 666 397 91 42 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2503 2997 Size: 2503 494 BsiEI CG_ry'CG Cuts at: 0 2362 2997 Size: 2362 635 BsiHKAI G_wGCw'C Cuts at: 0 181 326 479 691 836 1995 2018 2997 Size: 181 145 153 212 145 1159 23 979 Fragments arranged by size: 1159 979 212 181 153 145 145 23 BsmI GAATG_Cn' Cuts at: 0 1209 2021 2997 Size: 1209 812 976 Fragments arranged by size: 1209 976 812 BsmBI CGTCTCn'nnnn_ Cuts at: 0 369 2349 2997 Size: 369 1980 648 Fragments arranged by size: 1980 648 369 Bsp1286I G_dGCh'C Cuts at: 0 49 79 123 181 326 406 479 691 Size: 49 30 44 58 145 80 73 212 Cuts at: 691 836 1434 1995 2018 2655 2893 2997 Size: 145 598 561 23 637 238 104 Fragments arranged by size: 637 598 561 238 212 145 145 104 80 73 58 49 44 30 23 BspEI T'CCGG_A Cuts at: 0 548 2217 2997 Size: 548 1669 780 Fragments arranged by size: 1669 780 548 BspHI T'CATG_A Cuts at: 0 933 1619 2997 Size: 933 686 1378 Fragments arranged by size: 1378 933 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 558 704 2612 2997 Size: 558 146 1908 385 Fragments arranged by size: 1908 558 385 146 BsrBI CCG'CTC Cuts at: 0 429 1684 2997 Size: 429 1255 1313 Fragments arranged by size: 1313 1255 429 BsrDI GCAATG_nn' Cuts at: 0 650 2450 2997 Size: 650 1800 547 Fragments arranged by size: 1800 650 547 BsrFI r'CCGG_y Cuts at: 0 38 2877 2997 Size: 38 2839 120 Fragments arranged by size: 2839 120 38 BsrGI T'GTAC_A Cuts at: 0 776 2997 Size: 776 2221 BssHII G'CGCG_C Cuts at: 0 1000 2997 Size: 1000 1997 BssSI C'ACGA_G Cuts at: 0 835 1756 2734 2755 2997 Size: 835 921 978 21 242 Fragments arranged by size: 978 921 835 242 21 BstAPI GCAn_nnn'nTGC Cuts at: 0 857 2997 Size: 857 2140 BstBI TT'CG_AA Cuts at: 0 2109 2158 2674 2997 Size: 2109 49 516 323 Fragments arranged by size: 2109 516 323 49 BstEII G'GTnAC_C Cuts at: 0 1588 1972 2997 Size: 1588 384 1025 Fragments arranged by size: 1588 1025 384 BstXI CCAn_nnnn'nTGG Cuts at: 0 1353 2174 2997 Size: 1353 821 823 Fragments arranged by size: 1353 823 821 BstYI r'GATC_y Cuts at: 0 150 535 839 1781 2997 Size: 150 385 304 942 1216 Fragments arranged by size: 1216 942 385 304 150 Bsu36I CC'TnA_GG Cuts at: 0 50 2997 Size: 50 2947 BtgI C'CryG_G Cuts at: 0 562 2647 2783 2997 Size: 562 2085 136 214 Fragments arranged by size: 2085 562 214 136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1556 2997 Size: 1556 1441 BtsI GCAGTG_nn' Cuts at: 0 100 2179 2997 Size: 100 2079 818 Fragments arranged by size: 2079 818 100 DraIII CAC_nnn'GTG Cuts at: 0 1217 2997 Size: 1217 1780 DrdI GACnn_nn'nnGTC Cuts at: 0 95 2997 Size: 95 2902 EaeI y'GGCC_r Cuts at: 0 156 345 575 1796 2359 2997 Size: 156 189 230 1221 563 638 Fragments arranged by size: 1221 638 563 230 189 156 EagI C'GGCC_G Cuts at: 0 2359 2997 Size: 2359 638 EarI CTCTTCn'nnn_ Cuts at: 0 935 1360 1409 1841 1979 2266 2809 2997 Size: 935 425 49 432 138 287 543 188 Fragments arranged by size: 935 543 432 425 287 188 138 49 EcoICRI GAG'CTC Cuts at: 0 689 2016 2997 Size: 689 1327 981 Fragments arranged by size: 1327 981 689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 26 460 520 741 865 1979 2156 2587 Size: 26 434 60 221 124 1114 177 431 Cuts at: 2587 2924 2997 Size: 337 73 Fragments arranged by size: 1114 434 431 337 221 177 124 73 60 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 53 442 2842 2997 Size: 53 389 2400 155 Fragments arranged by size: 2400 389 155 53 EcoO109I rG'GnC_Cy Cuts at: 0 75 76 112 120 402 1398 2178 2652 Size: 75 1 36 8 282 996 780 474 Cuts at: 2652 2997 Size: 345 Fragments arranged by size: 996 780 474 345 282 75 36 8 1 EcoRI G'AATT_C Cuts at: 0 589 2997 Size: 589 2408 EcoRV GAT'ATC Cuts at: 0 2385 2997 Size: 2385 612 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 816 2997 Size: 816 2181 HaeII r_GCGC'y Cuts at: 0 1793 1854 2997 Size: 1793 61 1143 Fragments arranged by size: 1793 1143 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 96 128 214 246 1647 1679 2392 2424 Size: 96 32 86 32 1401 32 713 32 Cuts at: 2424 2997 Size: 573 Fragments arranged by size: 1401 713 573 96 86 32 32 32 32 HincII GTy'rAC Cuts at: 0 285 2532 2997 Size: 285 2247 465 Fragments arranged by size: 2247 465 285 HindIII A'AGCT_T Cuts at: 0 1865 2997 Size: 1865 1132 MfeI C'AATT_G Cuts at: 0 1160 2997 Size: 1160 1837 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 167 1050 2489 2997 Size: 167 883 1439 508 Fragments arranged by size: 1439 883 508 167 MscI TGG'CCA Cuts at: 0 158 347 577 1798 2997 Size: 158 189 230 1221 1199 Fragments arranged by size: 1221 1199 230 189 158 MslI CAynn'nnrTG Cuts at: 0 263 938 2038 2230 2317 2990 2992 2997 Size: 263 675 1100 192 87 673 2 5 Fragments arranged by size: 1100 675 673 263 192 87 5 2 MspA1I CmG'CkG Cuts at: 0 462 2565 2955 2997 Size: 462 2103 390 42 Fragments arranged by size: 2103 462 390 42 NaeI GCC'GGC Cuts at: 0 40 2879 2997 Size: 40 2839 118 Fragments arranged by size: 2839 118 40 NcoI C'CATG_G Cuts at: 0 2647 2997 Size: 2647 350 NgoMIV G'CCGG_C Cuts at: 0 38 2877 2997 Size: 38 2839 120 Fragments arranged by size: 2839 120 38 NspI r_CATG'y Cuts at: 0 258 2997 Size: 258 2739 PflMI CCAn_nnn'nTGG Cuts at: 0 354 1515 2899 2997 Size: 354 1161 1384 98 Fragments arranged by size: 1384 1161 354 98 PfoI T'CCnGG_A Cuts at: 0 785 1352 1772 2997 Size: 785 567 420 1225 Fragments arranged by size: 1225 785 567 420 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1243 1275 1591 1623 2599 2631 2997 Size: 1243 32 316 32 976 32 366 Fragments arranged by size: 1243 976 366 316 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 112 1398 2178 2997 Size: 112 1286 780 819 Fragments arranged by size: 1286 819 780 112 PspOMI G'GGCC_C Cuts at: 0 75 119 402 2651 2997 Size: 75 44 283 2249 346 Fragments arranged by size: 2249 346 283 75 44 PstI C_TGCA'G Cuts at: 0 134 680 701 1680 2623 2997 Size: 134 546 21 979 943 374 Fragments arranged by size: 979 943 546 374 134 21 PvuII CAG'CTG Cuts at: 0 2565 2955 2997 Size: 2565 390 42 Fragments arranged by size: 2565 390 42 SacI G_AGCT'C Cuts at: 0 691 2018 2997 Size: 691 1327 979 Fragments arranged by size: 1327 979 691 SalI G'TCGA_C Cuts at: 0 283 2997 Size: 283 2714 SapI GCTCTTCn'nnn_ Cuts at: 0 1841 2809 2997 Size: 1841 968 188 Fragments arranged by size: 1841 968 188 ScaI AGT'ACT Cuts at: 0 2494 2683 2997 Size: 2494 189 314 Fragments arranged by size: 2494 314 189 SfcI C'TryA_G Cuts at: 0 130 676 697 1372 1676 2187 2499 2619 Size: 130 546 21 675 304 511 312 120 Cuts at: 2619 2997 Size: 378 Fragments arranged by size: 675 546 511 378 312 304 130 120 21 SmaI CCC'GGG Cuts at: 0 118 2997 Size: 118 2879 SmlI C'TyrA_G Cuts at: 0 100 505 1876 2860 2997 Size: 100 405 1371 984 137 Fragments arranged by size: 1371 984 405 137 100 SphI G_CATG'C Cuts at: 0 258 2997 Size: 258 2739 StuI AGG'CCT Cuts at: 0 1449 1944 2997 Size: 1449 495 1053 Fragments arranged by size: 1449 1053 495 StyI C'CwwG_G Cuts at: 0 233 333 2647 2997 Size: 233 100 2314 350 Fragments arranged by size: 2314 350 233 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2143 2997 Size: 2143 854 TatI w'GTAC_w Cuts at: 0 291 776 2222 2492 2681 2997 Size: 291 485 1446 270 189 316 Fragments arranged by size: 1446 485 316 291 270 189 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1121 1153 1203 1235 2527 2559 2997 Size: 1121 32 50 32 1292 32 438 Fragments arranged by size: 1292 1121 438 50 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 166 340 876 1908 2997 Size: 166 174 536 1032 1089 Fragments arranged by size: 1089 1032 536 174 166 XmaI C'CCGG_G Cuts at: 0 116 2997 Size: 116 2881 XmnI GAAnn'nnTTC Cuts at: 0 1172 1207 2997 Size: 1172 35 1790 Fragments arranged by size: 1790 1172 35 Enzymes that do cut: AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SalI SapI ScaI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiWI BstZ17I ClaI CspCI DraI EciI FseI FspI FspAI HpaI KasI KpnI MluI NarI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI