################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AgeI AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbsI BcgI BciVI BclI BglII BlnI BpuAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AflII 1546 CTTAAG C^TTAAG AflIII 2018 ACACGT A^C[GA][CT]GT Alw44I 1747 GTGCAC G^TGCAC AlwNI 865 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1747 GTGCAC G^TGCAC BamHI 1206 GGATCC G^GATCC BbrPI 2019 CACGTG CAC^GTG BbvCI 954 GCTGAGG CCTCAGC(-5/-2) BfrI 1546 CTTAAG C^TTAAG BlpI 1594 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1594 GCTAAGC GC^T[ACGT]AGC BsaAI 2019 CACGTG [CT]AC^GT[GA] BsaI 1098 GAGACC GGTCTC(1/5) BsaWI 1948 TCCGGT [AT]^CCGG[AT] BsgI 347 GTGCAG GTGCAG(16/14) BsmBI 634 GAGACG CGTCTC(1/5) BsrDI 846 CATTGC GCAATG(2/0) CelII 1594 GCTAAGC GC^T[ACGT]AGC EcoNI 834 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG MaeII 2020 ACGT A^CGT MseI 1547 TTAA T^TAA PmaCI 2019 CACGTG CAC^GTG PmlI 2019 CACGTG CAC^GTG SexAI 1685 ACCTGGT A^CC[AT]GGT SmlI 1546 CTTAAG C^T[CT][GA]AG Tru9I 1547 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 588 GTAC GT^AC 1074 GTAC AocI 778 CCTGAGG CC^T[ACGT]AGG 1240 CCTGAGG Aor51HI 351 AGCGCT AGC^GCT 1130 AGCGCT ApaI 284 GGGCCC GGGCC^C 399 GGGCCC AviII 382 TGCGCA TGC^GCA 1554 TGCGCA BfaI 715 CTAG C^TAG 2144 CTAG BsmAI 634 GAGAC GTCTC(1/5) 1098 GAGAC BspMI 476 ACCTGC ACCTGC(4/8) 560 ACCTGC BsrFI 162 GCCGGC [GA]^CCGG[CT] 878 GCCGGC BssHII 135 GCGCGC G^CGCGC 434 GCGCGC BstYI 510 GGATCT [GA]^GATC[CT] 1206 GGATCC Bsu36I 778 CCTGAGG CC^T[ACGT]AGG 1240 CCTGAGG Cfr10I 162 GCCGGC [GA]^CCGG[CT] 878 GCCGGC DraIII 1684 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 1741 CACCCAGTG DrdI 75 GACCCAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1756 GACAACTGCGTC Eco47III 351 AGCGCT AGC^GCT 1130 AGCGCT Eco81I 778 CCTGAGG CC^T[ACGT]AGG 1240 CCTGAGG FspI 382 TGCGCA TGC^GCA 1554 TGCGCA MaeI 715 CTAG C^TAG 2144 CTAG MflI 510 GGATCT [GA]^GATC[CT] 1206 GGATCC NaeI 162 GCCGGC GCC^GGC 878 GCCGGC NcoI 1178 CCATGG C^CATGG 1965 CCATGG NgoMIV 162 GCCGGC G^CCGGC 878 GCCGGC PshAI 593 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1732 GACCTGAGTC RsaI 588 GTAC GT^AC 1074 GTAC SapI 724 GCTCTTC GCTCTTC(1/4) 1029 GCTCTTC StuI 1282 AGGCCT AGG^CCT 1640 AGGCCT TaqI 1529 TCGA T^CGA 1955 TCGA TthHB8I 1529 TCGA T^CGA 1955 TCGA XcmI 1178 CCATGGAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2166 CCAAGACAAGACTGG XhoII 510 GGATCT [GA]^GATC[CT] 1206 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TfiI TseI Tsp45I TspRI XmaCI XmaI