(Linear) (Six-base) MAPSORT of: KIEE3949.seq Check: 444 from: 1 to: 2208 KIEE3949.orf With 182 enzymes: SgfI * March 22, 2012 16:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 569 2208 Size: 569 1639 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 712 818 1926 2208 Size: 712 106 1108 282 Fragments arranged by size: 1108 712 282 106 AfeI AGC'GCT Cuts at: 0 353 1132 2208 Size: 353 779 1076 Fragments arranged by size: 1076 779 353 AflII C'TTAA_G Cuts at: 0 1546 2208 Size: 1546 662 AflIII A'CryG_T Cuts at: 0 2018 2208 Size: 2018 190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1776 2208 Size: 1776 432 AlwNI CAG_nnn'CTG Cuts at: 0 870 2208 Size: 870 1338 ApaI G_GGCC'C Cuts at: 0 288 403 2208 Size: 288 115 1805 Fragments arranged by size: 1805 288 115 ApaLI G'TGCA_C Cuts at: 0 1747 2208 Size: 1747 461 AvaI C'yCGr_G Cuts at: 0 48 87 280 403 1565 1907 1985 2189 Size: 48 39 193 123 1162 342 78 204 Cuts at: 2189 2208 Size: 19 Fragments arranged by size: 1162 342 204 193 123 78 48 39 19 BamHI G'GATC_C Cuts at: 0 1206 2208 Size: 1206 1002 BanI G'GyrC_C Cuts at: 0 93 159 256 683 1928 2025 2153 2184 Size: 93 66 97 427 1245 97 128 31 Cuts at: 2184 2208 Size: 24 Fragments arranged by size: 1245 427 128 97 97 93 66 31 24 BanII G_rGCy'C Cuts at: 0 65 288 296 403 467 1065 1301 1538 Size: 65 223 8 107 64 598 236 237 Cuts at: 1538 1802 1886 2141 2208 Size: 264 84 255 67 Fragments arranged by size: 598 264 255 237 236 223 107 84 67 65 64 8 BbeI G_GCGC'C Cuts at: 0 97 163 260 687 1932 2208 Size: 97 66 97 427 1245 276 Fragments arranged by size: 1245 427 276 97 97 66 BbvCI CC'TCA_GC Cuts at: 0 955 2208 Size: 955 1253 BglI GCCn_nnn'nGGC Cuts at: 0 145 407 830 1060 2208 Size: 145 262 423 230 1148 Fragments arranged by size: 1148 423 262 230 145 BlpI GC'TnA_GC Cuts at: 0 1595 2208 Size: 1595 613 Bme1580I G_kGCm'C Cuts at: 0 288 403 1751 2004 2028 2187 2208 Size: 288 115 1348 253 24 159 21 Fragments arranged by size: 1348 288 253 159 115 24 21 BmrI ACTGGGnnnn_n' Cuts at: 0 372 657 688 1738 2208 Size: 372 285 31 1050 470 Fragments arranged by size: 1050 470 372 285 31 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 315 1276 2208 Size: 315 961 932 Fragments arranged by size: 961 932 315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 652 691 1366 1493 1627 2208 Size: 652 39 675 127 134 581 Fragments arranged by size: 675 652 581 134 127 39 Bpu10I CC'TnA_GC Cuts at: 0 59 955 980 1454 2030 2208 Size: 59 896 25 474 576 178 Fragments arranged by size: 896 576 474 178 59 25 BsaI GGTCTCn'nnnn_ Cuts at: 0 1092 2208 Size: 1092 1116 BsaAI yAC'GTr Cuts at: 0 2021 2208 Size: 2021 187 BsaHI Gr'CG_yC Cuts at: 0 94 160 257 446 637 684 1929 2208 Size: 94 66 97 189 191 47 1245 279 Fragments arranged by size: 1245 279 191 189 97 94 66 47 BsaWI w'CCGG_w Cuts at: 0 1948 2208 Size: 1948 260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 326 356 2032 2062 2208 Size: 326 30 1676 30 146 Fragments arranged by size: 1676 326 146 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1 733 796 820 1825 2101 2104 2208 Size: 1 732 63 24 1005 276 3 104 Fragments arranged by size: 1005 732 276 104 63 24 3 1 BseYI C'CCAG_C Cuts at: 0 77 207 396 1082 1086 1422 1465 1570 Size: 77 130 189 686 4 336 43 105 Cuts at: 1570 1803 2116 2208 Size: 233 313 92 Fragments arranged by size: 686 336 313 233 189 130 105 92 77 43 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 368 2208 Size: 368 1840 BsiEI CG_ry'CG Cuts at: 0 19 149 167 248 2208 Size: 19 130 18 81 1960 Fragments arranged by size: 1960 130 81 19 18 BsiHKAI G_wGCw'C Cuts at: 0 1187 1751 2036 2208 Size: 1187 564 285 172 Fragments arranged by size: 1187 564 285 172 BsmBI CGTCTCn'nnnn_ Cuts at: 0 628 2208 Size: 628 1580 Bsp1286I G_dGCh'C Cuts at: 0 65 288 296 403 467 1065 1187 1301 Size: 65 223 8 107 64 598 122 114 Cuts at: 1301 1538 1751 1802 1886 2004 2028 2036 2141 Size: 237 213 51 84 118 24 8 105 Cuts at: 2141 2187 2208 Size: 46 21 Fragments arranged by size: 598 237 223 213 122 118 114 107 105 84 65 64 51 46 24 21 8 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 485 569 2208 Size: 485 84 1639 Fragments arranged by size: 1639 485 84 BsrBI CCG'CTC Cuts at: 0 1395 1502 1620 2208 Size: 1395 107 118 588 Fragments arranged by size: 1395 588 118 107 BsrDI GCAATG_nn' Cuts at: 0 845 2208 Size: 845 1363 BsrFI r'CCGG_y Cuts at: 0 162 878 2208 Size: 162 716 1330 Fragments arranged by size: 1330 716 162 BssHII G'CGCG_C Cuts at: 0 135 434 2208 Size: 135 299 1774 Fragments arranged by size: 1774 299 135 BstYI r'GATC_y Cuts at: 0 510 1206 2208 Size: 510 696 1002 Fragments arranged by size: 1002 696 510 Bsu36I CC'TnA_GG Cuts at: 0 779 1241 2208 Size: 779 462 967 Fragments arranged by size: 967 779 462 BtgI C'CryG_G Cuts at: 0 167 1178 1965 2208 Size: 167 1011 787 243 Fragments arranged by size: 1011 787 243 167 BtsI GCAGTG_nn' Cuts at: 0 703 880 2208 Size: 703 177 1328 Fragments arranged by size: 1328 703 177 DraIII CAC_nnn'GTG Cuts at: 0 1689 1746 2208 Size: 1689 57 462 Fragments arranged by size: 1689 462 57 DrdI GACnn_nn'nnGTC Cuts at: 0 81 1762 2208 Size: 81 1681 446 Fragments arranged by size: 1681 446 81 EaeI y'GGCC_r Cuts at: 0 16 146 164 245 2208 Size: 16 130 18 81 1963 Fragments arranged by size: 1963 130 81 18 16 EagI C'GGCC_G Cuts at: 0 16 146 164 245 2208 Size: 16 130 18 81 1963 Fragments arranged by size: 1963 130 81 18 16 EarI CTCTTCn'nnn_ Cuts at: 0 731 797 1036 1153 1273 2090 2096 2208 Size: 731 66 239 117 120 817 6 112 Fragments arranged by size: 817 731 239 120 117 112 66 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 346 442 2208 Size: 346 96 1766 Fragments arranged by size: 1766 346 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 652 691 712 818 1366 1493 1627 1926 Size: 652 39 21 106 548 127 134 299 Cuts at: 1926 2208 Size: 282 Fragments arranged by size: 652 548 299 282 134 127 106 39 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 838 2208 Size: 838 1370 EcoO109I rG'GnC_Cy Cuts at: 0 53 105 284 285 310 323 400 485 Size: 53 52 179 1 25 13 77 85 Cuts at: 485 627 775 831 867 1866 2208 Size: 142 148 56 36 999 342 Fragments arranged by size: 999 342 179 148 142 85 77 56 53 52 36 25 13 1 FspI TGC'GCA Cuts at: 0 384 1556 2208 Size: 384 1172 652 Fragments arranged by size: 1172 652 384 HaeII r_GCGC'y Cuts at: 0 97 163 175 190 208 260 355 687 Size: 97 66 12 15 18 52 95 332 Cuts at: 687 1134 1703 1932 1998 2208 Size: 447 569 229 66 210 Fragments arranged by size: 569 447 332 229 210 97 95 66 66 52 18 15 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 326 358 1091 1109 1123 1141 2208 Size: 326 32 733 18 14 18 1067 Fragments arranged by size: 1067 733 326 32 18 18 14 KasI G'GCGC_C Cuts at: 0 93 159 256 683 1928 2208 Size: 93 66 97 427 1245 280 Fragments arranged by size: 1245 427 280 97 93 66 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1370 1385 2208 Size: 1370 15 823 Fragments arranged by size: 1370 823 15 MspA1I CmG'CkG Cuts at: 0 21 81 520 954 1086 1380 1449 1465 Size: 21 60 439 434 132 294 69 16 Cuts at: 1465 1560 1829 2208 Size: 95 269 379 Fragments arranged by size: 439 434 379 294 269 132 95 69 60 21 16 NaeI GCC'GGC Cuts at: 0 164 880 2208 Size: 164 716 1328 Fragments arranged by size: 1328 716 164 NarI GG'CG_CC Cuts at: 0 94 160 257 684 1929 2208 Size: 94 66 97 427 1245 279 Fragments arranged by size: 1245 427 279 97 94 66 NcoI C'CATG_G Cuts at: 0 1178 1965 2208 Size: 1178 787 243 Fragments arranged by size: 1178 787 243 NgoMIV G'CCGG_C Cuts at: 0 162 878 2208 Size: 162 716 1330 Fragments arranged by size: 1330 716 162 PasI CC'CwG_GG Cuts at: 0 1149 2208 Size: 1149 1059 PfoI T'CCnGG_A Cuts at: 0 440 623 1343 1766 2208 Size: 440 183 720 423 442 Fragments arranged by size: 720 442 440 423 183 PmlI CAC'GTG Cuts at: 0 2021 2208 Size: 2021 187 PpuMI rG'GwC_Cy Cuts at: 0 53 310 323 627 867 1866 2208 Size: 53 257 13 304 240 999 342 Fragments arranged by size: 999 342 304 257 240 53 13 PshAI GACnn'nnGTC Cuts at: 0 597 1736 2208 Size: 597 1139 472 Fragments arranged by size: 1139 597 472 PspOMI G'GGCC_C Cuts at: 0 284 399 2208 Size: 284 115 1809 Fragments arranged by size: 1809 284 115 PstI C_TGCA'G Cuts at: 0 482 566 875 1230 1448 2075 2208 Size: 482 84 309 355 218 627 133 Fragments arranged by size: 627 482 355 309 218 133 84 PvuII CAG'CTG Cuts at: 0 81 520 954 1086 1380 1449 1465 1560 Size: 81 439 434 132 294 69 16 95 Cuts at: 1560 2208 Size: 648 Fragments arranged by size: 648 439 434 294 132 95 81 69 16 SanDI GG'GwC_CC Cuts at: 0 53 310 2208 Size: 53 257 1898 Fragments arranged by size: 1898 257 53 SapI GCTCTTCn'nnn_ Cuts at: 0 731 1036 2208 Size: 731 305 1172 Fragments arranged by size: 1172 731 305 SexAI A'CCwGG_T Cuts at: 0 1685 2208 Size: 1685 523 SfcI C'TryA_G Cuts at: 0 478 562 871 1004 1226 1444 1824 2071 Size: 478 84 309 133 222 218 380 247 Cuts at: 2071 2208 Size: 137 Fragments arranged by size: 478 380 309 247 222 218 137 133 84 SfoI GGC'GCC Cuts at: 0 95 161 258 685 1930 2208 Size: 95 66 97 427 1245 278 Fragments arranged by size: 1245 427 278 97 95 66 SmaI CCC'GGG Cuts at: 0 50 89 405 1567 2208 Size: 50 39 316 1162 641 Fragments arranged by size: 1162 641 316 50 39 SmlI C'TyrA_G Cuts at: 0 1546 2208 Size: 1546 662 SrfI GCCC'GGGC Cuts at: 0 1567 2208 Size: 1567 641 StuI AGG'CCT Cuts at: 0 1284 1642 2208 Size: 1284 358 566 Fragments arranged by size: 1284 566 358 StyI C'CwwG_G Cuts at: 0 1178 1285 1965 2208 Size: 1178 107 680 243 Fragments arranged by size: 1178 680 243 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1696 1757 2208 Size: 1696 61 451 Fragments arranged by size: 1696 451 61 TatI w'GTAC_w Cuts at: 0 587 2208 Size: 587 1621 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2032 2064 2208 Size: 2032 32 144 Fragments arranged by size: 2032 144 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1185 2173 2208 Size: 1185 988 35 Fragments arranged by size: 1185 988 35 XmaI C'CCGG_G Cuts at: 0 48 87 403 1565 2208 Size: 48 39 316 1162 643 Fragments arranged by size: 1162 643 316 48 39 Enzymes that do cut: AarI AcuI AfeI AflII AflIII AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MspA1I NaeI NarI NcoI NgoMIV PasI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SanDI SapI SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI BsiWI BsmI BspEI BspHI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MslI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ZraI