################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BseRI BsiHKAI BsiWI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco52I EcoO109I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MluI MluNI MroI MscI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 485 GTCGAC GT^[AC][GT]AC AccII 147 CGCG CG^CG AflIII 466 ACATGT A^C[GA][CT]GT AocI 2909 CCTCAGG CC^T[ACGT]AGG ApaI 299 GGGCCC GGGCC^C AseI 2773 ATTAAT AT^TAAT AsnI 2773 ATTAAT AT^TAAT Asp700I 2353 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 190 GGACC G^G[AT]CC BbsI 1845 GTCTTC GAAGAC(2/6) BpuAI 1845 GTCTTC GAAGAC(2/6) BsiEI 12 CGACCG CG[GA][CT]^CG BsmBI 2420 CGTCTC CGTCTC(1/5) Bsp1407I 1194 TGTACA T^GTACA BspHI 681 TCATGA T^CATGA BspLU11I 466 ACATGT A^CATGT BsrFI 14 ACCGGC [GA]^CCGG[CT] BsrGI 1194 TGTACA T^GTACA BstEII 2758 GGTAACC G^GT[ACGT]ACC BstPI 2758 GGTAACC G^GT[ACGT]ACC BstUI 147 CGCG CG^CG Bsu36I 2909 CCTCAGG CC^T[ACGT]AGG Cfr10I 14 ACCGGC [GA]^CCGG[CT] DrdI 927 GACTAGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2909 CCTCAGG CC^T[ACGT]AGG EcoO65I 2758 GGTAACC G^GT[ACGT]ACC EcoT14I 3056 CCATGG C^C[AT][AT]GG HgaI 143 GCGTC GACGC(5/10) HincII 485 GTCGAC GT[CT]^[GA]AC HindII 485 GTCGAC GT[CT]^[GA]AC MaeII 1666 ACGT A^CGT MfeI 365 CAATTG C^AATTG MunI 365 CAATTG C^AATTG MvnI 147 CGCG CG^CG NcoI 3056 CCATGG C^CATGG NheI 1033 GCTAGC G^CTAGC PflFI 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2811 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2773 ATTAAT AT^TAAT RcaI 681 TCATGA T^CATGA SalI 485 GTCGAC G^TCGAC ScaI 1810 AGTACT AGT^ACT SmlI 1046 CTCAAG C^T[CT][GA]AG SspBI 1194 TGTACA T^GTACA StyI 3056 CCATGG C^C[AT][AT]GG TaqI 486 TCGA T^CGA Tth111I 34 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 486 TCGA T^CGA XmnI 2353 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 446 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG 1901 CAGCCCCTG Aor51HI 839 AGCGCT AGC^GCT 1872 AGCGCT AvaI 1800 CTCGGG C^[CT]CG[GA]G 2090 CCCGAG BclI 636 TGATCA T^GATCA 2142 TGATCA BglII 1140 AGATCT A^GATCT 2043 AGATCT BsaBI 673 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2038 GATCAAGATC BsaI 3 GGTCTC GGTCTC(1/5) 39 GGTCTC BsmFI 254 GTCCC GGGAC(10/14) 2826 GTCCC BsoBI 1800 CTCGGG C^[CT]CG[GA]G 2090 CCCGAG BspMI 756 ACCTGC ACCTGC(4/8) 2652 GCAGGT BsrDI 1562 CATTGC GCAATG(2/0) 1760 GCAATG BstXI 258 CCATAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 564 CCAATACTGTGG DraI 273 TTTAAA TTT^AAA 2793 TTTAAA DsaI 193 CCGTGG C^C[GA][CT]GG 3056 CCATGG EarI 2525 CTCTTC CTCTTC(1/4) 431 GAAGAG Eco47III 839 AGCGCT AGC^GCT 1872 AGCGCT EcoNI 1336 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2491 CCTCATATAGG FauI 111 CCCGC CCCGC(4/6) 1353 GCGGG FbaI 636 TGATCA T^GATCA 2142 TGATCA HaeII 839 AGCGCT [GA]GCGC^[CT] 1872 AGCGCT HindIII 158 AAGCTT A^AGCTT 1293 AAGCTT Ksp632I 2525 CTCTTC CTCTTC(1/4) 431 GAAGAG MamI 673 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2038 GATCAAGATC MslI 259 CATAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1969 CATATGTATG NdeI 632 CATATG CA^TATG 1969 CATATG NspI 466 ACATGT [GA]CATG^[CT] 505 ACATGC SfaNI 153 GCATC GCATC(5/9) 1513 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BanI BanII BbvCI BbvI BfaI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII