(Circular) (Six-base) MAPSORT of: pFN21AE3347.seq Check: 8977 from: 1 to: 7786 pFN21AE3347 7786 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2770 2770 Size: 7786 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5596 278 Size: 53 83 186 936 4060 2468 Fragments arranged by size: 4060 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2491 5129 1937 Size: 554 2638 4594 Fragments arranged by size: 4594 2638 554 Acc65I G'GTAC_C Cuts at: 5107 5107 Size: 7786 AclI AA'CG_TT Cuts at: 1563 5913 6286 1563 Size: 4350 373 3063 Fragments arranged by size: 4350 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2949 4304 4349 5849 7233 7501 1632 Size: 1317 1355 45 1500 1384 268 1917 Fragments arranged by size: 1917 1500 1384 1355 1317 268 45 AfeI AGC'GCT Cuts at: 2846 3879 2846 Size: 1033 6753 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7589 AflIII A'CryG_T Cuts at: 1203 2471 6685 7662 1203 Size: 1268 4214 977 1327 Fragments arranged by size: 4214 1327 1268 977 AgeI A'CCGG_T Cuts at: 6617 6617 Size: 7786 AhdI GACnn_n'nnGTC Cuts at: 6515 6515 Size: 7786 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7786 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7786 AlwNI CAG_nnn'CTG Cuts at: 2456 3911 5529 7101 2456 Size: 1455 1618 1572 3141 Fragments arranged by size: 3141 1618 1572 1455 ApaI G_GGCC'C Cuts at: 2308 2308 Size: 7786 ApaLI G'TGCA_C Cuts at: 5843 6999 5843 Size: 1156 6630 ApoI r'AATT_y Cuts at: 1493 2881 3307 3627 3743 3994 4987 5095 5321 Size: 1388 426 320 116 251 993 108 226 Cuts at: 5321 5473 7582 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1388 993 426 320 251 226 152 116 108 AseI AT'TA_AT Cuts at: 160 1032 4779 6340 160 Size: 872 3747 1561 1606 Fragments arranged by size: 3747 1606 1561 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7786 AvaI C'yCGr_G Cuts at: 1870 1943 3805 4095 5111 1870 Size: 73 1862 290 1016 4545 Fragments arranged by size: 4545 1862 1016 290 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4848 4881 4848 Size: 33 7753 BamHI G'GATC_C Cuts at: 5116 5483 5116 Size: 367 7419 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 3964 5016 5107 6562 Size: 325 217 627 768 1409 1052 91 1455 Cuts at: 6562 618 Size: 1842 Fragments arranged by size: 1842 1455 1409 1052 768 627 325 217 91 BanII G_rGCy'C Cuts at: 1391 2308 3364 4496 5105 1391 Size: 917 1056 1132 609 4072 Fragments arranged by size: 4072 1132 1056 917 609 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3843 928 Size: 971 1944 4871 Fragments arranged by size: 4871 1944 971 BbvCI CC'TCA_GC Cuts at: 3274 3843 3889 3274 Size: 569 46 7171 Fragments arranged by size: 7171 569 46 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5977 6011 1903 Size: 34 4040 34 3678 Fragments arranged by size: 4040 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5678 6888 5678 Size: 1210 6576 BclI T'GATC_A Cuts at: 1548 2641 4147 1548 Size: 1093 1506 5187 Fragments arranged by size: 5187 1506 1093 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6397 136 Size: 107 122 71 631 605 4725 1525 Fragments arranged by size: 4725 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3145 4048 7781 3145 Size: 903 3733 3150 Fragments arranged by size: 3733 3150 903 BlpI GC'TnA_GC Cuts at: 5203 5203 Size: 7786 Bme1580I G_kGCm'C Cuts at: 1790 2139 2308 2323 4684 5847 7003 1790 Size: 349 169 15 2361 1163 1156 2573 Fragments arranged by size: 2573 2361 1163 1156 349 169 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7786 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5517 6475 442 Size: 416 53 83 399 24 4100 958 1753 Fragments arranged by size: 4100 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3042 1056 Size: 1986 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4641 4121 Size: 520 7266 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 3057 3336 3597 3811 4167 4543 6446 Size: 815 1003 279 261 214 356 376 1903 Cuts at: 6446 1239 Size: 2579 Fragments arranged by size: 2579 1903 1003 815 376 356 279 261 214 Bpu10I CC'TnA_GC Cuts at: 2977 3128 3274 3572 3843 3889 4636 2977 Size: 151 146 298 271 46 747 6127 Fragments arranged by size: 6127 747 298 271 151 146 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3036 5911 6776 7074 3036 Size: 2875 865 298 3748 Fragments arranged by size: 3748 2875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 2050 6449 882 Size: 617 515 36 4399 2219 Fragments arranged by size: 4399 2219 617 515 36 BsaAI yAC'GTr Cuts at: 493 1204 5577 493 Size: 711 4373 2702 Fragments arranged by size: 4373 2702 711 BsaBI GATnn'nnATC Cuts at: 2682 4047 7780 2682 Size: 1365 3733 2688 Fragments arranged by size: 3733 2688 1365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5593 5975 6610 275 Size: 53 83 186 936 4060 382 635 1451 Fragments arranged by size: 4060 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6219 6617 6891 7038 7449 6219 Size: 398 274 147 411 6556 Fragments arranged by size: 6556 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 3155 3185 4205 Size: 30 165 30 627 30 1095 30 1020 Cuts at: 4205 4235 4541 4571 4588 4618 1178 Size: 30 306 30 17 30 4346 Fragments arranged by size: 4346 1095 1020 627 306 165 30 30 30 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7786 BseYI C'CCAG_C Cuts at: 2103 4610 6989 7485 2103 Size: 2507 2379 496 2404 Fragments arranged by size: 2507 2404 2379 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2847 2883 3128 1163 Size: 573 1111 36 245 5821 Fragments arranged by size: 5821 1111 573 245 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 5236 5997 7025 1540 Size: 275 186 19 3216 761 1028 2301 Fragments arranged by size: 3216 2301 1028 761 275 186 19 BsiHKAI G_wGCw'C Cuts at: 5847 5932 7003 5847 Size: 85 1071 6630 Fragments arranged by size: 6630 1071 85 BsmI GAATG_Cn' Cuts at: 3694 4284 4381 4701 5297 5390 3694 Size: 590 97 320 596 93 6090 Fragments arranged by size: 6090 596 590 320 97 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4431 4431 Size: 7786 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2308 2323 3364 4496 4684 5105 Size: 399 349 169 15 1041 1132 188 421 Cuts at: 5105 5847 5932 7003 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 1041 742 421 399 349 188 169 85 15 BspEI T'CCGG_A Cuts at: 7449 7449 Size: 7786 BspHI T'CATG_A Cuts at: 2686 5675 7405 2686 Size: 2989 1730 3067 Fragments arranged by size: 3067 2989 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2770 4648 5141 844 Size: 1926 1878 493 3489 Fragments arranged by size: 3489 1926 1878 493 BsrBI CCG'CTC Cuts at: 1394 1823 5673 1394 Size: 429 3850 3507 Fragments arranged by size: 3850 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3566 3772 6281 6455 65 Size: 1183 2318 206 2509 174 1396 Fragments arranged by size: 2509 2318 1396 1183 206 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2019 6430 6617 1700 Size: 110 209 4411 187 2869 Fragments arranged by size: 4411 2869 209 187 110 BsrGI T'GTAC_A Cuts at: 96 3199 96 Size: 3103 4683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7786 BssSI C'ACGA_G Cuts at: 5846 6858 5846 Size: 1012 6774 BstAPI GCAn_nnn'nTGC Cuts at: 794 3524 3911 4457 794 Size: 2730 387 546 4123 Fragments arranged by size: 4123 2730 546 387 BstBI TT'CG_AA Cuts at: 6590 6590 Size: 7786 BstEII G'GTnAC_C Cuts at: 1184 4763 1184 Size: 3579 4207 BstXI CCAn_nnnn'nTGG Cuts at: 2270 2576 2270 Size: 306 7480 BstYI r'GATC_y Cuts at: 1972 2624 3145 3809 3947 4048 5116 5483 5868 Size: 652 521 664 138 101 1068 367 385 Cuts at: 5868 5885 7337 7423 7435 7781 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1068 664 652 521 385 367 346 138 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4915 4915 Size: 7786 BtgI C'CryG_G Cuts at: 513 1065 2004 2198 5061 513 Size: 552 939 194 2863 3238 Fragments arranged by size: 3238 2863 939 552 194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7047 Fragments arranged by size: 7047 683 56 BtsI GCAGTG_nn' Cuts at: 3085 4226 4740 5302 6096 6116 3085 Size: 1141 514 562 794 20 4755 Fragments arranged by size: 4755 1141 794 562 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7786 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7751 DraI TTT'AAA Cuts at: 2280 4800 5090 2280 Size: 2520 290 4976 Fragments arranged by size: 4976 2520 290 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2938 6793 809 Size: 507 1622 3855 1802 Fragments arranged by size: 3855 1802 1622 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5233 6122 7751 Size: 54 1425 50 275 18 3403 889 1629 Cuts at: 7751 8 Size: 43 Fragments arranged by size: 3403 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5233 1537 Size: 275 3421 4090 Fragments arranged by size: 4090 3421 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2431 4536 5716 1353 Size: 1078 2105 1180 3423 Fragments arranged by size: 3423 2105 1180 1078 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6373 6747 6893 387 Size: 1064 138 4784 374 146 1280 Fragments arranged by size: 4784 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2949 3057 3336 3597 3811 4167 Size: 393 422 895 108 279 261 214 356 Cuts at: 4167 4304 4349 4543 5849 6446 7233 7501 1239 Size: 137 45 194 1306 597 787 268 1524 Fragments arranged by size: 1524 1306 895 787 597 422 393 356 279 268 261 214 194 137 108 45 EcoNI CCTnn'n_nnAGG Cuts at: 3345 4500 3345 Size: 1155 6631 EcoRI G'AATT_C Cuts at: 5095 5473 5095 Size: 378 7408 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2676 2676 Size: 7786 FspI TGC'GCA Cuts at: 6292 6292 Size: 7786 HaeII r_GCGC'y Cuts at: 1678 2848 3881 6933 1678 Size: 1170 1033 3052 2531 Fragments arranged by size: 3052 2531 1170 1033 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2648 2680 3149 Size: 32 135 32 458 32 586 32 469 Cuts at: 3149 3181 3939 3971 4541 4573 6427 6459 6501 Size: 32 758 32 570 32 1854 32 42 Cuts at: 6501 6533 1373 Size: 32 2626 Fragments arranged by size: 2626 1854 758 586 570 469 458 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2492 5130 5376 669 Size: 988 281 554 2638 246 3079 Fragments arranged by size: 3079 2638 988 554 281 246 HindIII A'AGCT_T Cuts at: 748 2163 3298 748 Size: 1415 1135 5236 Fragments arranged by size: 5236 1415 1135 HpaI GTT'AAC Cuts at: 5376 5376 Size: 7786 KpnI G_GTAC'C Cuts at: 5111 5111 Size: 7786 MfeI C'AATT_G Cuts at: 2370 5385 2370 Size: 3015 4771 MluI A'CGCG_T Cuts at: 7662 7662 Size: 7786 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4424 6900 7084 1305 Size: 3119 2476 184 2007 Fragments arranged by size: 3119 2476 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5964 Fragments arranged by size: 5964 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2268 3978 5744 6103 6262 518 Size: 648 1102 1710 1766 359 159 2042 Fragments arranged by size: 2042 1766 1710 1102 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2119 3278 3524 3893 5202 5879 7027 7272 Size: 513 1159 246 369 1309 677 1148 245 Cuts at: 7272 1606 Size: 2120 Fragments arranged by size: 2120 1309 1159 1148 677 513 369 246 245 NcoI C'CATG_G Cuts at: 513 1065 2004 5061 513 Size: 552 939 3057 3238 Fragments arranged by size: 3238 3057 939 552 NdeI CA'TA_TG Cuts at: 387 2638 3975 387 Size: 2251 1337 4198 Fragments arranged by size: 4198 2251 1337 NheI G'CTAG_C Cuts at: 1052 3038 1052 Size: 1986 5800 NotI GC'GGCC_GC Cuts at: 5233 5233 Size: 7786 NspI r_CATG'y Cuts at: 1132 1229 2475 2514 5144 6689 1132 Size: 97 1246 39 2630 1545 2229 Fragments arranged by size: 2630 2229 1545 1246 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7786 PciI A'CATG_T Cuts at: 2471 6685 2471 Size: 4214 3572 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7786 PfoI T'CCnGG_A Cuts at: 2886 3016 2886 Size: 130 7656 PmeI GTTT'AAAC Cuts at: 5090 5090 Size: 7786 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5855 5887 7368 7400 5855 Size: 32 1481 32 6241 Fragments arranged by size: 6241 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4820 1613 Size: 3207 4579 PsiI TTA'TAA Cuts at: 2403 3759 5356 2403 Size: 1356 1597 4833 Fragments arranged by size: 4833 1597 1356 PspOMI G'GGCC_C Cuts at: 2304 2304 Size: 7786 PstI C_TGCA'G Cuts at: 830 2147 3529 3555 3907 4735 5138 830 Size: 1317 1382 26 352 828 403 3478 Fragments arranged by size: 3478 1382 1317 828 403 352 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7786 PvuII CAG'CTG Cuts at: 3278 3524 3893 3278 Size: 246 369 7171 Fragments arranged by size: 7171 369 246 SalI G'TCGA_C Cuts at: 1936 2490 5128 1936 Size: 554 2638 4594 Fragments arranged by size: 4594 2638 554 SbfI CC_TGCA'GG Cuts at: 5138 5138 Size: 7786 ScaI AGT'ACT Cuts at: 3817 6034 7746 3817 Size: 2217 1712 3857 Fragments arranged by size: 3857 2217 1712 SfcI C'TryA_G Cuts at: 826 1045 2143 2790 3342 3513 3525 3551 3903 Size: 219 1098 647 552 171 12 26 352 Cuts at: 3903 4731 5134 6269 6950 7141 826 Size: 828 403 1135 681 191 1471 Fragments arranged by size: 1471 1135 1098 828 681 647 552 403 352 219 191 171 26 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7786 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7786 SmaI CCC'GGG Cuts at: 1872 5113 1872 Size: 3241 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3051 5890 6791 7053 820 Size: 197 926 1108 2839 901 262 1553 Fragments arranged by size: 2839 1553 1108 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5577 493 Size: 5084 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7786 SphI G_CATG'C Cuts at: 1132 5144 1132 Size: 4012 3774 SspI AAT'ATT Cuts at: 5 52 5710 5 Size: 47 5658 2081 Fragments arranged by size: 5658 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7786 StyI C'CwwG_G Cuts at: 513 1065 2004 5061 5225 513 Size: 552 939 3057 164 3074 Fragments arranged by size: 3074 3057 939 552 164 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4727 4868 5813 5830 5983 6168 6613 Size: 1255 3147 141 945 17 153 185 445 Cuts at: 6613 325 Size: 1498 Fragments arranged by size: 3147 1498 1255 945 445 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3199 3815 5008 Size: 274 80 33 51 1443 1222 616 1193 Cuts at: 5008 6032 7744 96 Size: 1024 1712 138 Fragments arranged by size: 1712 1443 1222 1193 1024 616 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3012 3044 3090 3122 4205 4237 7746 7778 3012 Size: 32 46 32 1083 32 3509 32 3020 Fragments arranged by size: 3509 3020 1083 46 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2042 1316 Size: 726 7060 XbaI T'CTAG_A Cuts at: 5122 5122 Size: 7786 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7786 XmaI C'CCGG_G Cuts at: 1870 5111 1870 Size: 3241 4545 XmnI GAAnn'nnTTC Cuts at: 4362 5915 4362 Size: 1553 6233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5594 276 Size: 53 83 186 936 4060 2468 Fragments arranged by size: 4060 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI