(Linear) (Six-base) MAPSORT of: KIEE3347.seq Check: 9601 from: 1 to: 3081 KIEE3347.orf With 182 enzymes: SgfI * July 3, 2012 17:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 765 3081 Size: 765 2316 AccI GT'mk_AC Cuts at: 0 486 3081 Size: 486 2595 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 944 2299 2344 3081 Size: 944 1355 45 737 Fragments arranged by size: 1355 944 737 45 AfeI AGC'GCT Cuts at: 0 841 1874 3081 Size: 841 1033 1207 Fragments arranged by size: 1207 1033 841 AflIII A'CryG_T Cuts at: 0 466 3081 Size: 466 2615 AlwNI CAG_nnn'CTG Cuts at: 0 451 1906 3081 Size: 451 1455 1175 Fragments arranged by size: 1455 1175 451 ApaI G_GGCC'C Cuts at: 0 303 3081 Size: 303 2778 ApoI r'AATT_y Cuts at: 0 876 1302 1622 1738 1989 2982 3081 Size: 876 426 320 116 251 993 99 Fragments arranged by size: 993 876 426 320 251 116 99 AseI AT'TA_AT Cuts at: 0 2774 3081 Size: 2774 307 AvaI C'yCGr_G Cuts at: 0 1800 2090 3081 Size: 1800 290 991 Fragments arranged by size: 1800 991 290 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2843 2876 3081 Size: 2843 33 205 Fragments arranged by size: 2843 205 33 BanI G'GyrC_C Cuts at: 0 550 1959 3011 3081 Size: 550 1409 1052 70 Fragments arranged by size: 1409 1052 550 70 BanII G_rGCy'C Cuts at: 0 303 1359 2491 3081 Size: 303 1056 1132 590 Fragments arranged by size: 1132 1056 590 303 BbsI GAAGACnn'nnnn_ Cuts at: 0 1838 3081 Size: 1838 1243 BbvCI CC'TCA_GC Cuts at: 0 1269 1838 1884 3081 Size: 1269 569 46 1197 Fragments arranged by size: 1269 1197 569 46 BclI T'GATC_A Cuts at: 0 636 2142 3081 Size: 636 1506 939 Fragments arranged by size: 1506 939 636 BglII A'GATC_T Cuts at: 0 1140 2043 3081 Size: 1140 903 1038 Fragments arranged by size: 1140 1038 903 Bme1580I G_kGCm'C Cuts at: 0 134 303 318 2679 3081 Size: 134 169 15 2361 402 Fragments arranged by size: 2361 402 169 134 15 BmtI G_CTAG'C Cuts at: 0 1037 3081 Size: 1037 2044 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2116 2636 3081 Size: 2116 520 445 Fragments arranged by size: 2116 520 445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 1052 1331 1592 1806 2162 2538 3081 Size: 49 1003 279 261 214 356 376 543 Fragments arranged by size: 1003 543 376 356 279 261 214 49 Bpu10I CC'TnA_GC Cuts at: 0 972 1123 1269 1567 1838 1884 2631 3081 Size: 972 151 146 298 271 46 747 450 Fragments arranged by size: 972 747 450 298 271 151 146 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1031 3081 Size: 1031 2050 BsaI GGTCTCn'nnnn_ Cuts at: 0 9 45 3081 Size: 9 36 3036 Fragments arranged by size: 3036 36 9 BsaBI GATnn'nnATC Cuts at: 0 677 2042 3081 Size: 677 1365 1039 Fragments arranged by size: 1365 1039 677 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 55 1150 1180 2200 2230 2536 2566 Size: 25 30 1095 30 1020 30 306 30 Cuts at: 2566 2583 2613 3081 Size: 17 30 468 Fragments arranged by size: 1095 1020 468 306 30 30 30 30 30 25 17 BseYI C'CCAG_C Cuts at: 0 98 2605 3081 Size: 98 2507 476 Fragments arranged by size: 2507 476 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 842 878 1123 3081 Size: 842 36 245 1958 Fragments arranged by size: 1958 842 245 36 BsiEI CG_ry'CG Cuts at: 0 15 3081 Size: 15 3066 BsmI GAATG_Cn' Cuts at: 0 1689 2279 2376 2696 3081 Size: 1689 590 97 320 385 Fragments arranged by size: 1689 590 385 320 97 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2426 3081 Size: 2426 655 Bsp1286I G_dGCh'C Cuts at: 0 134 303 318 1359 2491 2679 3081 Size: 134 169 15 1041 1132 188 402 Fragments arranged by size: 1132 1041 402 188 169 134 15 BspHI T'CATG_A Cuts at: 0 681 3081 Size: 681 2400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 765 2643 3081 Size: 765 1878 438 Fragments arranged by size: 1878 765 438 BsrDI GCAATG_nn' Cuts at: 0 1561 1767 3081 Size: 1561 206 1314 Fragments arranged by size: 1561 1314 206 BsrFI r'CCGG_y Cuts at: 0 14 3081 Size: 14 3067 BsrGI T'GTAC_A Cuts at: 0 1194 3081 Size: 1194 1887 BstAPI GCAn_nnn'nTGC Cuts at: 0 1519 1906 2452 3081 Size: 1519 387 546 629 Fragments arranged by size: 1519 629 546 387 BstEII G'GTnAC_C Cuts at: 0 2758 3081 Size: 2758 323 BstXI CCAn_nnnn'nTGG Cuts at: 0 265 571 3081 Size: 265 306 2510 Fragments arranged by size: 2510 306 265 BstYI r'GATC_y Cuts at: 0 619 1140 1804 1942 2043 3081 Size: 619 521 664 138 101 1038 Fragments arranged by size: 1038 664 619 521 138 101 Bsu36I CC'TnA_GG Cuts at: 0 2910 3081 Size: 2910 171 BtgI C'CryG_G Cuts at: 0 193 3056 3081 Size: 193 2863 25 Fragments arranged by size: 2863 193 25 BtsI GCAGTG_nn' Cuts at: 0 1080 2221 2735 3081 Size: 1080 1141 514 346 Fragments arranged by size: 1141 1080 514 346 DraI TTT'AAA Cuts at: 0 275 2795 3081 Size: 275 2520 286 Fragments arranged by size: 2520 286 275 DrdI GACnn_nn'nnGTC Cuts at: 0 933 3081 Size: 933 2148 EarI CTCTTCn'nnn_ Cuts at: 0 426 2531 3081 Size: 426 2105 550 Fragments arranged by size: 2105 550 426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 944 1052 1331 1592 1806 2162 2299 Size: 49 895 108 279 261 214 356 137 Cuts at: 2299 2344 2538 3081 Size: 45 194 543 Fragments arranged by size: 895 543 356 279 261 214 194 137 108 49 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 1340 2495 3081 Size: 1340 1155 586 Fragments arranged by size: 1340 1155 586 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 671 3081 Size: 671 2410 HaeII r_GCGC'y Cuts at: 0 843 1876 3081 Size: 843 1033 1205 Fragments arranged by size: 1205 1033 843 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 25 57 643 675 1144 1176 1934 1966 Size: 25 32 586 32 469 32 758 32 Cuts at: 1966 2536 2568 3081 Size: 570 32 513 Fragments arranged by size: 758 586 570 513 469 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 0 487 3081 Size: 487 2594 HindIII A'AGCT_T Cuts at: 0 158 1293 3081 Size: 158 1135 1788 Fragments arranged by size: 1788 1135 158 MfeI C'AATT_G Cuts at: 0 365 3081 Size: 365 2716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2419 3081 Size: 2419 662 MslI CAynn'nnrTG Cuts at: 0 263 1973 3081 Size: 263 1710 1108 Fragments arranged by size: 1710 1108 263 MspA1I CmG'CkG Cuts at: 0 114 1273 1519 1888 3081 Size: 114 1159 246 369 1193 Fragments arranged by size: 1193 1159 369 246 114 NcoI C'CATG_G Cuts at: 0 3056 3081 Size: 3056 25 NdeI CA'TA_TG Cuts at: 0 633 1970 3081 Size: 633 1337 1111 Fragments arranged by size: 1337 1111 633 NheI G'CTAG_C Cuts at: 0 1033 3081 Size: 1033 2048 NspI r_CATG'y Cuts at: 0 470 509 3081 Size: 470 39 2572 Fragments arranged by size: 2572 470 39 PciI A'CATG_T Cuts at: 0 466 3081 Size: 466 2615 PfoI T'CCnGG_A Cuts at: 0 881 1011 3081 Size: 881 130 2070 Fragments arranged by size: 2070 881 130 PshAI GACnn'nnGTC Cuts at: 0 2815 3081 Size: 2815 266 PsiI TTA'TAA Cuts at: 0 398 1754 3081 Size: 398 1356 1327 Fragments arranged by size: 1356 1327 398 PspOMI G'GGCC_C Cuts at: 0 299 3081 Size: 299 2782 PstI C_TGCA'G Cuts at: 0 142 1524 1550 1902 2730 3081 Size: 142 1382 26 352 828 351 Fragments arranged by size: 1382 828 352 351 142 26 PvuII CAG'CTG Cuts at: 0 1273 1519 1888 3081 Size: 1273 246 369 1193 Fragments arranged by size: 1273 1193 369 246 SalI G'TCGA_C Cuts at: 0 485 3081 Size: 485 2596 ScaI AGT'ACT Cuts at: 0 1812 3081 Size: 1812 1269 SfcI C'TryA_G Cuts at: 0 138 785 1337 1508 1520 1546 1898 2726 Size: 138 647 552 171 12 26 352 828 Cuts at: 2726 3081 Size: 355 Fragments arranged by size: 828 647 552 355 352 171 138 26 12 SmlI C'TyrA_G Cuts at: 0 1046 3081 Size: 1046 2035 StyI C'CwwG_G Cuts at: 0 3056 3081 Size: 3056 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2722 2863 3081 Size: 2722 141 218 Fragments arranged by size: 2722 218 141 TatI w'GTAC_w Cuts at: 0 1194 1810 3003 3081 Size: 1194 616 1193 78 Fragments arranged by size: 1194 1193 616 78 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1007 1039 1085 1117 2200 2232 3081 Size: 1007 32 46 32 1083 32 849 Fragments arranged by size: 1083 1007 849 46 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 37 3081 Size: 37 3044 XmnI GAAnn'nnTTC Cuts at: 0 2357 3081 Size: 2357 724 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlwNI ApaI ApoI AseI AvaI BaeI BanI BanII BbsI BbvCI BclI BglII Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI DrdI EarI Eco57MI EcoNI FalI HaeII Hin4I HincII HindIII MfeI MmeI MslI MspA1I NcoI NdeI NheI NspI PciI PfoI PshAI PsiI PspOMI PstI PvuII SalI ScaI SfcI SmlI StyI TaqII TatI TstI Tth111I XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI AvrII BamHI BbeI BcgI BciVI BfrBI BglI BlpI BmgBI BmrI BsaAI BsaHI BsaWI BseRI BsiHKAI BsiWI BspEI BsrBI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MluI MscI NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI PspXI PsrI PvuI RsrII SacI SacII SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI ZraI