(Circular) (Six-base) MAPSORT of: pFN21AE2343.seq Check: 6508 from: 1 to: 6664 pFN21AE2343 6664 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3526 3526 Size: 6664 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2067 4474 278 Size: 53 83 186 936 531 2407 2468 Fragments arranged by size: 2468 2407 936 531 186 83 53 AccI GT'mk_AC Cuts at: 1937 3015 4007 1937 Size: 1078 992 4594 Fragments arranged by size: 4594 1078 992 Acc65I G'GTAC_C Cuts at: 2499 3985 2499 Size: 1486 5178 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2819 4727 6111 6379 1632 Size: 1187 1908 1384 268 1917 Fragments arranged by size: 1917 1908 1384 1187 268 AfeI AGC'GCT Cuts at: 2189 3321 2189 Size: 1132 5532 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 2847 3961 5563 6540 1203 Size: 1644 1114 1602 977 1327 Fragments arranged by size: 1644 1602 1327 1114 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3338 1184 Size: 2154 4510 AlwNI CAG_nnn'CTG Cuts at: 2059 3703 4407 5979 2059 Size: 1644 704 1572 2744 Fragments arranged by size: 2744 1644 1572 704 ApaI G_GGCC'C Cuts at: 2389 2459 2598 2826 3061 3127 3165 3899 2389 Size: 70 139 228 235 66 38 734 5154 Fragments arranged by size: 5154 734 235 228 139 70 66 38 ApaLI G'TGCA_C Cuts at: 4721 5877 4721 Size: 1156 5508 ApoI r'AATT_y Cuts at: 1493 3973 4199 4351 6460 1493 Size: 2480 226 152 2109 1697 Fragments arranged by size: 2480 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2291 2471 2701 2825 3259 3501 3838 Size: 73 348 180 230 124 434 242 337 Cuts at: 3838 3989 1870 Size: 151 4545 Fragments arranged by size: 4545 434 348 337 242 230 180 151 124 73 AvrII C'CTAG_G Cuts at: 3899 3899 Size: 6664 BamHI G'GATC_C Cuts at: 2287 3994 4361 2287 Size: 1707 367 4590 Fragments arranged by size: 4590 1707 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 2467 2499 2506 2531 Size: 325 217 627 489 191 32 7 25 Cuts at: 2531 2737 2777 3353 3398 3538 3602 3985 5440 Size: 206 40 576 45 140 64 383 1455 Cuts at: 5440 618 Size: 1842 Fragments arranged by size: 1842 1455 627 576 489 383 325 217 206 191 140 64 45 40 32 25 7 BanII G_rGCy'C Cuts at: 1391 2298 2389 2399 2459 2598 2826 2859 3008 Size: 907 91 10 60 139 228 33 149 Cuts at: 3008 3036 3061 3127 3165 3899 3983 1391 Size: 28 25 66 38 734 84 4072 Fragments arranged by size: 4072 907 734 228 149 139 91 84 66 60 38 33 28 25 10 BbeI G_GCGC'C Cuts at: 2280 2471 2510 2535 3357 3402 2280 Size: 191 39 25 822 45 5542 Fragments arranged by size: 5542 822 191 45 39 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5693 BbvCI CC'TCA_GC Cuts at: 2599 2599 Size: 6664 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4855 4889 1903 Size: 34 50 34 2834 34 3678 Fragments arranged by size: 3678 2834 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2873 3375 3437 Size: 107 122 71 631 605 1201 502 62 Cuts at: 3437 5275 136 Size: 1838 1525 Fragments arranged by size: 1838 1525 1201 631 605 502 122 107 71 62 BglII A'GATC_T Cuts at: 6659 6659 Size: 6664 BlpI GC'TnA_GC Cuts at: 4081 4081 Size: 6664 Bme1580I G_kGCm'C Cuts at: 1790 2146 2389 2459 2598 2826 3061 3127 3165 Size: 356 243 70 139 228 235 66 38 Cuts at: 3165 3605 3773 3899 4725 5881 1790 Size: 440 168 126 826 1156 2573 Fragments arranged by size: 2573 1156 826 440 356 243 235 228 168 139 126 70 66 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2396 4395 5353 Size: 416 53 83 399 24 979 1999 958 Cuts at: 5353 442 Size: 1753 Fragments arranged by size: 1999 1753 979 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3050 3149 3236 3407 3893 5324 1239 Size: 1811 99 87 171 486 1431 2579 Fragments arranged by size: 2579 1811 1431 486 171 99 87 Bpu10I CC'TnA_GC Cuts at: 2599 3242 2599 Size: 643 6021 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2978 3188 4789 5654 5952 2978 Size: 210 1601 865 298 3690 Fragments arranged by size: 3690 1601 865 298 210 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5327 882 Size: 617 3828 2219 Fragments arranged by size: 3828 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3667 4455 493 Size: 711 2463 788 2702 Fragments arranged by size: 2702 2463 788 711 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2259 2277 2468 Size: 53 83 186 936 531 195 18 191 Cuts at: 2468 2507 2532 2963 2985 3354 3399 4471 4853 Size: 39 25 431 22 369 45 1072 382 Cuts at: 4853 5488 275 Size: 635 1451 Fragments arranged by size: 1451 1072 936 635 531 431 382 369 195 191 186 83 53 45 39 25 22 18 BsaWI w'CCGG_w Cuts at: 3956 5097 5495 5769 5916 6327 3956 Size: 1141 398 274 147 411 4293 Fragments arranged by size: 4293 1141 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2054 2084 2870 2900 2948 Size: 30 165 30 651 30 786 30 48 Cuts at: 2948 2978 3215 3245 1178 Size: 30 237 30 4597 Fragments arranged by size: 4597 786 651 237 165 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2972 3057 3233 3236 3410 3458 1186 Size: 873 913 85 176 3 174 48 4392 Fragments arranged by size: 4392 913 873 176 174 85 48 3 BseYI C'CCAG_C Cuts at: 2028 3325 3349 3720 3796 5867 6363 2028 Size: 1297 24 371 76 2071 496 2329 Fragments arranged by size: 2329 2071 1297 496 371 76 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3191 3785 1163 Size: 573 1455 594 4042 Fragments arranged by size: 4042 1455 594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2484 2559 2769 3441 3825 4114 Size: 275 186 483 75 210 672 384 289 Cuts at: 4114 4875 5903 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 672 483 384 289 275 210 186 75 BsiHKAI G_wGCw'C Cuts at: 4725 4810 5881 4725 Size: 85 1071 5508 Fragments arranged by size: 5508 1071 85 BsiWI C'GTAC_G Cuts at: 2850 2850 Size: 6664 BsmI GAATG_Cn' Cuts at: 4175 4268 4175 Size: 93 6571 BsmBI CGTCTCn'nnnn_ Cuts at: 2071 2071 Size: 6664 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2298 2389 2399 2459 2598 2826 Size: 399 356 152 91 10 60 139 228 Cuts at: 2826 2859 3008 3036 3061 3127 3165 3605 3773 Size: 33 149 28 25 66 38 440 168 Cuts at: 3773 3899 3983 4725 4810 5881 1391 Size: 126 84 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 356 228 168 152 149 139 126 91 85 84 66 60 38 33 28 25 10 BspEI T'CCGG_A Cuts at: 3956 6327 3956 Size: 2371 4293 BspHI T'CATG_A Cuts at: 4553 6283 4553 Size: 1730 4934 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3526 4019 844 Size: 2682 493 3489 Fragments arranged by size: 3489 2682 493 BsrBI CCG'CTC Cuts at: 1394 1823 3055 3544 3745 4551 1394 Size: 429 1232 489 201 806 3507 Fragments arranged by size: 3507 1232 806 489 429 201 BsrDI GCAATG_nn' Cuts at: 65 1248 2449 5159 5333 65 Size: 1183 1201 2710 174 1396 Fragments arranged by size: 2710 1396 1201 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 2438 2483 2734 3642 5308 5495 Size: 110 276 352 45 251 908 1666 187 Cuts at: 5495 1700 Size: 2869 Fragments arranged by size: 2869 1666 908 352 276 251 187 110 45 BsrGI T'GTAC_A Cuts at: 96 2610 96 Size: 2514 4150 BssHII G'CGCG_C Cuts at: 1925 2520 2655 3198 3411 3413 3443 3528 3732 Size: 595 135 543 213 2 30 85 204 Cuts at: 3732 1925 Size: 4857 Fragments arranged by size: 4857 595 543 213 204 135 85 30 2 BssSI C'ACGA_G Cuts at: 3069 4724 5736 3069 Size: 1655 1012 3997 Fragments arranged by size: 3997 1655 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2448 3703 794 Size: 1654 1255 3755 Fragments arranged by size: 3755 1654 1255 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 3112 3349 3919 3112 Size: 237 570 5857 Fragments arranged by size: 5857 570 237 BstYI r'GATC_y Cuts at: 1972 2287 3699 3994 4361 4746 4763 6215 6301 Size: 315 1412 295 367 385 17 1452 86 Cuts at: 6301 6313 6659 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1412 385 367 346 315 295 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2763 2871 2898 2952 3278 3302 3618 Size: 552 1698 108 27 54 326 24 316 Cuts at: 3618 3691 513 Size: 73 3486 Fragments arranged by size: 3486 1698 552 326 316 108 73 54 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5925 Fragments arranged by size: 5925 683 56 BtsI GCAGTG_nn' Cuts at: 2166 3179 4180 4974 4994 2166 Size: 1013 1001 794 20 3836 Fragments arranged by size: 3836 1013 1001 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2120 2155 2959 2994 529 Size: 35 1556 35 804 35 4199 Fragments arranged by size: 4199 1556 804 35 35 35 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2300 2481 Size: 54 1425 50 275 18 215 255 181 Cuts at: 2481 2556 2766 3438 3822 3885 4111 5000 6629 Size: 75 210 672 384 63 226 889 1629 Cuts at: 6629 8 Size: 43 Fragments arranged by size: 1629 1425 889 672 384 275 255 226 215 210 181 75 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2481 2556 2766 3438 3822 4111 1537 Size: 275 669 75 210 672 384 289 4090 Fragments arranged by size: 4090 672 669 384 289 275 210 75 EarI CTCTTCn'nnn_ Cuts at: 1353 4594 1353 Size: 3241 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3194 5251 5625 5771 387 Size: 1064 138 1605 2057 374 146 1280 Fragments arranged by size: 2057 1605 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2819 3050 3149 3236 3407 3893 4727 Size: 393 1187 231 99 87 171 486 834 Cuts at: 4727 5324 6111 6379 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1187 834 787 597 486 393 268 231 171 99 87 EcoNI CCTnn'n_nnAGG Cuts at: 3422 3422 Size: 6664 EcoO109I rG'GnC_Cy Cuts at: 2385 2386 2455 2595 2822 2866 2998 3058 3123 Size: 1 69 140 227 44 132 60 65 Cuts at: 3123 3124 3162 3868 3896 3903 2385 Size: 1 38 706 28 7 5146 Fragments arranged by size: 5146 706 227 140 132 69 65 60 44 38 28 7 1 1 EcoRI G'AATT_C Cuts at: 3973 4351 3973 Size: 378 6286 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 2191 2280 2471 2510 2535 2606 3215 3323 Size: 513 89 191 39 25 71 609 108 Cuts at: 3323 3357 3402 3491 3649 3674 3716 3761 3853 Size: 34 45 89 158 25 42 45 92 Cuts at: 3853 3884 5811 1678 Size: 31 1927 2531 Fragments arranged by size: 2531 1927 609 513 191 158 108 92 89 89 71 45 45 42 39 34 31 25 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2141 2173 2870 Size: 32 135 32 482 32 55 32 697 Cuts at: 2870 2902 2948 2980 5305 5337 5379 5411 1373 Size: 32 46 32 2325 32 42 32 2626 Fragments arranged by size: 2626 2325 697 482 135 55 46 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4008 4254 669 Size: 988 281 2070 246 3079 Fragments arranged by size: 3079 2070 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KasI G'GCGC_C Cuts at: 2276 2467 2506 2531 3353 3398 2276 Size: 191 39 25 822 45 5542 Fragments arranged by size: 5542 822 191 45 39 25 KpnI G_GTAC'C Cuts at: 2503 3989 2503 Size: 1486 5178 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 2847 6540 2847 Size: 3693 2971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2079 2199 5778 5962 1305 Size: 774 120 3579 184 2007 Fragments arranged by size: 3579 2007 774 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 2047 2302 3887 10 Size: 54 1425 343 215 255 1585 2787 Fragments arranged by size: 2787 1585 1425 343 255 215 54 MslI CAynn'nnrTG Cuts at: 518 1166 4622 4981 5140 518 Size: 648 3456 359 159 2042 Fragments arranged by size: 3456 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2608 2900 2954 3280 3304 3333 3349 3620 Size: 1002 292 54 326 24 29 16 271 Cuts at: 3620 3693 3709 3805 4080 4757 5905 6150 1606 Size: 73 16 96 275 677 1148 245 2120 Fragments arranged by size: 2120 1148 1002 677 326 292 275 271 245 96 73 54 29 24 16 16 NaeI GCC'GGC Cuts at: 2088 2440 2485 2736 3644 2088 Size: 352 45 251 908 5108 Fragments arranged by size: 5108 908 352 251 45 NarI GG'CG_CC Cuts at: 2277 2468 2507 2532 3354 3399 2277 Size: 191 39 25 822 45 5542 Fragments arranged by size: 5542 822 191 45 39 25 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6112 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NgoMIV G'CCGG_C Cuts at: 2086 2438 2483 2734 3642 2086 Size: 352 45 251 908 5108 Fragments arranged by size: 5108 908 352 251 45 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 3438 4111 3438 Size: 673 5991 NspI r_CATG'y Cuts at: 1132 1229 4022 5567 1132 Size: 97 2793 1545 2229 Fragments arranged by size: 2793 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2111 2242 2389 3119 1348 Size: 763 131 147 730 4893 Fragments arranged by size: 4893 763 730 147 131 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2098 1108 Size: 902 88 5674 Fragments arranged by size: 5674 902 88 PfoI T'CCnGG_A Cuts at: 3048 3048 Size: 6664 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2998 3868 2998 Size: 870 5794 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6664 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2385 2455 2594 2822 3057 3123 3161 3895 2385 Size: 70 139 228 235 66 38 734 5154 Fragments arranged by size: 5154 734 235 228 139 70 66 38 PstI C_TGCA'G Cuts at: 830 2571 3537 3708 3849 4016 830 Size: 1741 966 171 141 167 3478 Fragments arranged by size: 3478 1741 966 171 167 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 3349 3805 3349 Size: 456 6208 RsrII CG'GwC_CG Cuts at: 3282 3282 Size: 6664 SacII CC_GC'GG Cuts at: 2901 2955 3281 3305 3621 3694 2901 Size: 54 326 24 316 73 5871 Fragments arranged by size: 5871 326 316 73 54 24 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 2204 4912 6624 2204 Size: 2708 1712 2244 Fragments arranged by size: 2708 2244 1712 SfcI C'TryA_G Cuts at: 826 1045 2567 3533 3704 3845 4012 5147 5828 Size: 219 1522 966 171 141 167 1135 681 Cuts at: 5828 6019 826 Size: 191 1471 Fragments arranged by size: 1522 1471 1135 966 681 219 191 171 167 141 SfoI GGC'GCC Cuts at: 2278 2469 2508 2533 3355 3400 2278 Size: 191 39 25 822 45 5542 Fragments arranged by size: 5542 822 191 45 39 25 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 3642 1700 Size: 1942 4722 SmaI CCC'GGG Cuts at: 1872 2703 3261 3503 3840 3991 1872 Size: 831 558 242 337 151 4545 Fragments arranged by size: 4545 831 558 337 242 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 2993 3203 4768 5669 5931 820 Size: 197 926 1050 210 1565 901 262 1553 Fragments arranged by size: 1565 1553 1050 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 2346 1517 Size: 829 5835 StyI C'CwwG_G Cuts at: 513 1065 2325 2862 3888 3899 3912 4103 513 Size: 552 1260 537 1026 11 13 191 3074 Fragments arranged by size: 3074 1260 1026 552 537 191 13 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4691 4708 4861 5046 5491 325 Size: 1255 3111 17 153 185 445 1498 Fragments arranged by size: 3111 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 2610 4910 Size: 274 80 33 51 1443 225 408 2300 Cuts at: 4910 6622 96 Size: 1712 138 Fragments arranged by size: 2300 1712 1443 408 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3215 3247 6624 6656 3215 Size: 32 3377 32 3223 Fragments arranged by size: 3377 3223 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2869 2869 Size: 6664 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 2701 3259 3501 3838 3989 1870 Size: 831 558 242 337 151 4545 Fragments arranged by size: 4545 831 558 337 242 151 XmnI GAAnn'nnTTC Cuts at: 4793 4793 Size: 6664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2065 4472 276 Size: 53 83 186 936 531 2407 2468 Fragments arranged by size: 2468 2407 936 531 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SacI SanDI SapI SexAI SfiI SrfI SwaI