################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AvrII BbeI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsgI BsiWI BsmI BspDI BspEI BspHI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 1458 GAATTT [GA]^AATT[CT] ApoI 1458 GAATTT [GA]^AATT[CT] Asp700I 1458 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1646 TGCGCA TGC^GCA BalI 1883 TGGCCA TGG^CCA BanI 1318 GGCACC G^G[CT][GA]CC BbsI 868 GTCTTC GAAGAC(2/6) BciVI 1857 GTATCC GTATCC(6/5) BclI 1530 TGATCA T^GATCA BfaI 1417 CTAG C^TAG BpuAI 868 GTCTTC GAAGAC(2/6) BsaI 781 GAGACC GGTCTC(1/5) BsaWI 1826 ACCGGA [AT]^CCGG[AT] Bsp1407I 440 TGTACA T^GTACA BspLU11I 413 ACATGT A^CATGT BsrGI 440 TGTACA T^GTACA DrdI 217 GACTACGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1530 TGATCA T^GATCA FspI 1646 TGCGCA TGC^GCA HincII 907 GTTGAC GT[CT]^[GA]AC HindII 907 GTTGAC GT[CT]^[GA]AC KspI 1514 CCGCGG CCGC^GG MaeI 1417 CTAG C^TAG MluNI 1883 TGGCCA TGG^CCA MscI 1883 TGGCCA TGG^CCA NotI 376 GCGGCCGC GC^GGCCGC NspI 413 ACATGT [GA]CATG^[CT] PflFI 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 2310 CAGCTG CAG^CTG SacI 304 GAGCTC GAGCT^C SacII 1514 CCGCGG CCGC^GG SmlI 2140 CTCAAG C^T[CT][GA]AG SspBI 440 TGTACA T^GTACA StuI 159 AGGCCT AGG^CCT TfiI 829 GATTC G^A[AT]TC Tth111I 862 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1458 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 580 GACGTC GACGT^C 646 GACGTC AflIII 413 ACATGT A^C[GA][CT]GT 2264 ACGTGT AhdI 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1615 GACTGGCGGTC AspEI 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1615 GACTGGCGGTC AspHI 271 GTGCTC G[AT]GC[AT]^C 304 GAGCTC BamHI 44 GGATCC G^GATCC 1307 GGATCC BglII 764 AGATCT A^GATCT 1491 AGATCT BsiHKAI 271 GTGCTC G[AT]GC[AT]^C 304 GAGCTC BspMI 501 GCAGGT ACCTGC(4/8) 2355 GCAGGT BsrDI 1649 GCAATG GCAATG(2/0) 2408 GCAATG BssHII 655 GCGCGC G^CGCGC 657 GCGCGC BssSI 268 CTCGTG CACGAG(-5/-1) 1759 CTCGTG BstAPI 944 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2270 GCAAGGACTGC BstXI 1139 CCAGCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1371 CCACGTGGCTGG Eam1105I 88 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1615 GACTGGCGGTC MaeIII 514 GTGAC ^GT[ACGT]AC 2136 GTCAC SapI 927 GAAGAGC GCTCTTC(1/4) 2391 GCTCTTC SmaI 533 CCCGGG CCC^GGG 1302 CCCGGG Tsp45I 514 GTGAC ^GT[GC]AC 2136 GTCAC Tsp509I 431 AATT ^AATT 1459 AATT XmaCI 533 CCCGGG C^CCGGG 1302 CCCGGG XmaI 533 CCCGGG C^CCGGG 1302 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AvaI AvaII BanII BbrPI BbvCI BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I Van91I XcmI XhoII