(Circular) (Six-base) MAPSORT of: pFN21AB6293.seq Check: 8658 from: 1 to: 7123 pFN21AB6293 7123 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2497 4351 2497 Size: 1854 5269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2589 2655 4933 278 Size: 53 83 186 936 1053 66 2278 2468 Fragments arranged by size: 2468 2278 1053 936 186 83 66 53 AccI GT'mk_AC Cuts at: 1937 4466 1937 Size: 2529 4594 Acc65I G'GTAC_C Cuts at: 4444 4444 Size: 7123 AclI AA'CG_TT Cuts at: 1563 5250 5623 1563 Size: 3687 373 3063 Fragments arranged by size: 3687 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5186 6570 6838 1632 Size: 3554 1384 268 1917 Fragments arranged by size: 3554 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6926 AflIII A'CryG_T Cuts at: 1203 2418 4269 6022 6999 1203 Size: 1215 1851 1753 977 1327 Fragments arranged by size: 1851 1753 1327 1215 977 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 2098 3625 5852 2098 Size: 1527 2227 3369 Fragments arranged by size: 3369 2227 1527 AleI CACnn'nnGTG Cuts at: 1166 4253 1166 Size: 3087 4036 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4190 4220 4222 4252 4190 Size: 30 2 30 7061 Fragments arranged by size: 7061 30 30 2 AlwNI CAG_nnn'CTG Cuts at: 2146 2176 2257 2692 2979 4231 4375 4866 6438 Size: 30 81 435 287 1252 144 491 1572 Cuts at: 6438 2146 Size: 2831 Fragments arranged by size: 2831 1572 1252 491 435 287 144 81 30 ApaLI G'TGCA_C Cuts at: 5180 6336 5180 Size: 1156 5967 ApoI r'AATT_y Cuts at: 1493 3463 4432 4658 4810 6919 1493 Size: 1970 969 226 152 2109 1697 Fragments arranged by size: 2109 1970 1697 969 226 152 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2472 2538 3307 3740 4009 4448 1870 Size: 73 529 66 769 433 269 439 4545 Fragments arranged by size: 4545 769 529 439 433 269 73 66 BamHI G'GATC_C Cuts at: 2049 3312 4453 4820 2049 Size: 1263 1141 367 4352 Fragments arranged by size: 4352 1263 1141 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3323 4444 5899 618 Size: 325 217 627 1536 1121 1455 1842 Fragments arranged by size: 1842 1536 1455 1121 627 325 217 BanII G_rGCy'C Cuts at: 1391 2313 2550 2906 4062 4442 1391 Size: 922 237 356 1156 380 4072 Fragments arranged by size: 4072 1156 922 380 356 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2866 928 Size: 971 967 5185 Fragments arranged by size: 5185 971 967 BbvCI CC'TCA_GC Cuts at: 2030 2126 3089 3693 2030 Size: 96 963 604 5460 Fragments arranged by size: 5460 963 604 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5314 5348 1903 Size: 34 3377 34 3678 Fragments arranged by size: 3678 3377 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3873 5015 6225 3873 Size: 1142 1210 4771 Fragments arranged by size: 4771 1210 1142 BclI T'GATC_A Cuts at: 1548 3535 1548 Size: 1987 5136 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5734 136 Size: 107 122 71 631 605 4062 1525 Fragments arranged by size: 4062 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2769 3496 7118 2769 Size: 727 3622 2774 Fragments arranged by size: 3622 2774 727 BlpI GC'TnA_GC Cuts at: 4540 4540 Size: 7123 Bme1580I G_kGCm'C Cuts at: 1790 3326 4306 5184 6340 1790 Size: 1536 980 878 1156 2573 Fragments arranged by size: 2573 1536 1156 980 878 BmgBI CAC'GTC Cuts at: 1318 2562 2968 3451 1318 Size: 1244 406 483 4990 Fragments arranged by size: 4990 1244 483 406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3241 4854 5812 Size: 416 53 83 399 24 1824 1613 958 Cuts at: 5812 442 Size: 1753 Fragments arranged by size: 1824 1753 1613 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 2320 Size: 7123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2597 2629 2714 2758 5783 1239 Size: 1358 32 85 44 3025 2579 Fragments arranged by size: 3025 2579 1358 85 44 32 Bpu10I CC'TnA_GC Cuts at: 2030 2126 3089 3693 2030 Size: 96 963 604 5460 Fragments arranged by size: 5460 963 604 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4130 5248 6113 6411 4130 Size: 1118 865 298 4842 Fragments arranged by size: 4842 1118 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 5786 882 Size: 617 1281 3006 2219 Fragments arranged by size: 3006 2219 1281 617 BsaAI yAC'GTr Cuts at: 493 1204 2155 2599 3379 4914 493 Size: 711 951 444 780 1535 2702 Fragments arranged by size: 2702 1535 951 780 711 444 BsaBI GATnn'nnATC Cuts at: 7117 7117 Size: 7123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2586 2652 4139 4930 Size: 53 83 186 936 1053 66 1487 791 Cuts at: 4930 5312 5947 275 Size: 382 635 1451 Fragments arranged by size: 1487 1451 1053 936 791 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 3831 5556 5954 6228 6375 6786 3831 Size: 1725 398 274 147 411 4168 Fragments arranged by size: 4168 1725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 2348 2378 4190 Size: 30 165 30 906 30 9 30 1812 Cuts at: 4190 4220 4250 1178 Size: 30 30 4051 Fragments arranged by size: 4051 1812 906 165 30 30 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2594 3941 3944 3947 4214 1186 Size: 949 459 1347 3 3 267 4095 Fragments arranged by size: 4095 1347 949 459 267 3 3 BseYI C'CCAG_C Cuts at: 2824 3101 3844 4077 6326 6822 2824 Size: 277 743 233 2249 496 3125 Fragments arranged by size: 3125 2249 743 496 277 233 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6550 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2385 2891 3397 3629 4157 4254 Size: 275 186 384 506 506 232 528 97 Cuts at: 4254 4573 5334 6362 1540 Size: 319 761 1028 2301 Fragments arranged by size: 2301 1028 761 528 506 506 384 319 275 232 186 97 BsiHKAI G_wGCw'C Cuts at: 2280 2313 5184 5269 6340 2280 Size: 33 2871 85 1071 3063 Fragments arranged by size: 3063 2871 1071 85 33 BsmI GAATG_Cn' Cuts at: 4634 4727 4634 Size: 93 7030 BsmBI CGTCTCn'nnnn_ Cuts at: 2423 2920 4196 2423 Size: 497 1276 5350 Fragments arranged by size: 5350 1276 497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2280 2313 2550 2906 3326 4062 4306 Size: 399 490 33 237 356 420 736 244 Cuts at: 4306 4442 5184 5269 6340 1391 Size: 136 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 736 490 420 399 356 244 237 136 85 33 BspEI T'CCGG_A Cuts at: 6786 6786 Size: 7123 BspHI T'CATG_A Cuts at: 5012 6742 5012 Size: 1730 5393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2497 4351 4478 844 Size: 1653 1854 127 3489 Fragments arranged by size: 3489 1854 1653 127 BsrBI CCG'CTC Cuts at: 1394 1823 2299 2746 2887 2937 3169 4164 5010 Size: 429 476 447 141 50 232 995 846 Cuts at: 5010 1394 Size: 3507 Fragments arranged by size: 3507 995 846 476 447 429 232 141 50 BsrDI GCAATG_nn' Cuts at: 65 1248 3661 4420 5618 5792 65 Size: 1183 2413 759 1198 174 1396 Fragments arranged by size: 2413 1396 1198 1183 759 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2569 3012 3637 4152 4156 5767 5954 Size: 110 759 443 625 515 4 1611 187 Cuts at: 5954 1700 Size: 2869 Fragments arranged by size: 2869 1611 759 625 515 443 187 110 4 BsrGI T'GTAC_A Cuts at: 96 2445 96 Size: 2349 4774 BssHII G'CGCG_C Cuts at: 1925 2660 2662 1925 Size: 735 2 6386 Fragments arranged by size: 6386 735 2 BssSI C'ACGA_G Cuts at: 2273 3764 5183 6195 2273 Size: 1491 1419 1012 3201 Fragments arranged by size: 3201 1491 1419 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2955 4281 794 Size: 2161 1326 3636 Fragments arranged by size: 3636 2161 1326 BstBI TT'CG_AA Cuts at: 5927 5927 Size: 7123 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7123 BstXI CCAn_nnnn'nTGG Cuts at: 3151 3383 3151 Size: 232 6891 BstYI r'GATC_y Cuts at: 1972 2049 2136 2769 3312 3496 4292 4453 4820 Size: 77 87 633 543 184 796 161 367 Cuts at: 4820 5205 5222 6674 6760 6772 7118 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 796 633 543 385 367 346 184 161 87 86 77 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2371 2907 3318 3457 3519 Size: 552 939 60 307 536 411 139 62 Cuts at: 3519 513 Size: 4117 Fragments arranged by size: 4117 939 552 536 411 307 139 62 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3057 487 Size: 683 56 1831 4553 Fragments arranged by size: 4553 1831 683 56 BtsI GCAGTG_nn' Cuts at: 2953 3100 4639 5433 5453 2953 Size: 147 1539 794 20 4623 Fragments arranged by size: 4623 1539 794 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7088 DraI TTT'AAA Cuts at: 4427 4427 Size: 7123 DraIII CAC_nnn'GTG Cuts at: 2046 2089 3330 4270 2046 Size: 43 1241 940 4899 Fragments arranged by size: 4899 1241 940 43 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2228 6130 809 Size: 507 912 3902 1802 Fragments arranged by size: 3902 1802 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2382 2888 Size: 54 1425 50 275 18 376 176 506 Cuts at: 2888 3207 3394 3888 4154 4417 4570 5459 7088 Size: 319 187 494 266 263 153 889 1629 Cuts at: 7088 8 Size: 43 Fragments arranged by size: 1629 1425 889 506 494 376 319 275 266 263 187 176 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2382 2888 3394 4154 4570 1537 Size: 275 570 506 506 760 416 4090 Fragments arranged by size: 4090 760 570 506 506 416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2927 4403 5053 1353 Size: 869 705 1476 650 3423 Fragments arranged by size: 3423 1476 869 705 650 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2146 2222 2252 5710 6084 6230 Size: 1064 138 557 76 30 3458 374 146 Cuts at: 6230 387 Size: 1280 Fragments arranged by size: 3458 1280 1064 557 374 146 138 76 30 EcoICRI GAG'CTC Cuts at: 2311 2311 Size: 7123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2597 2629 2714 2758 5186 5783 6570 Size: 393 965 32 85 44 2428 597 787 Cuts at: 6570 6838 1239 Size: 268 1524 Fragments arranged by size: 2428 1524 965 787 597 393 268 85 44 32 EcoO109I rG'GnC_Cy Cuts at: 2530 2689 3019 4023 4132 2530 Size: 159 330 1004 109 5521 Fragments arranged by size: 5521 1004 330 159 109 EcoRI G'AATT_C Cuts at: 4432 4810 4432 Size: 378 6745 FspI TGC'GCA Cuts at: 3653 5629 3653 Size: 1976 5147 HaeII r_GCGC'y Cuts at: 1678 2576 3566 3775 6270 1678 Size: 898 990 209 2495 2531 Fragments arranged by size: 2531 2495 990 898 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2084 2116 2309 2341 2348 Size: 32 135 32 512 32 193 32 7 Cuts at: 2348 2380 3333 3365 3611 3643 5764 5796 5838 Size: 32 953 32 246 32 2121 32 42 Cuts at: 5838 5870 1373 Size: 32 2626 Fragments arranged by size: 2626 2121 953 512 246 193 135 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 2914 4467 4713 669 Size: 988 281 976 1553 246 3079 Fragments arranged by size: 3079 1553 988 976 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 4713 4713 Size: 7123 KpnI G_GTAC'C Cuts at: 4448 4448 Size: 7123 MfeI C'AATT_G Cuts at: 4722 4722 Size: 7123 MluI A'CGCG_T Cuts at: 6999 6999 Size: 7123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2061 2340 2831 3312 6237 6421 1305 Size: 756 279 491 481 2925 184 2007 Fragments arranged by size: 2925 2007 756 491 481 279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3890 10 Size: 54 1425 343 2058 3243 Fragments arranged by size: 3243 2058 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2483 2861 4253 4361 5081 5440 5599 Size: 648 1317 378 1392 108 720 359 159 Cuts at: 5599 518 Size: 2042 Fragments arranged by size: 2042 1392 1317 720 648 378 359 159 108 MspA1I CmG'CkG Cuts at: 1606 3226 3521 3692 4317 4539 5216 6364 6609 Size: 1620 295 171 625 222 677 1148 245 Cuts at: 6609 1606 Size: 2120 Fragments arranged by size: 2120 1620 1148 677 625 295 245 222 171 NaeI GCC'GGC Cuts at: 2571 3014 4158 2571 Size: 443 1144 5536 Fragments arranged by size: 5536 1144 443 NcoI C'CATG_G Cuts at: 513 1065 2004 2064 2907 3457 513 Size: 552 939 60 843 550 4179 Fragments arranged by size: 4179 939 843 552 550 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NgoMIV G'CCGG_C Cuts at: 2569 3012 4156 2569 Size: 443 1144 5536 Fragments arranged by size: 5536 1144 443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7123 NotI GC'GGCC_GC Cuts at: 2382 4570 2382 Size: 2188 4935 NspI r_CATG'y Cuts at: 1132 1229 2422 4481 6026 1132 Size: 97 1193 2059 1545 2229 Fragments arranged by size: 2229 2059 1545 1193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7123 PciI A'CATG_T Cuts at: 2418 6022 2418 Size: 3604 3519 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 2257 3621 4122 1108 Size: 981 168 1364 501 4109 Fragments arranged by size: 4109 1364 981 501 168 PfoI T'CCnGG_A Cuts at: 2139 2774 2139 Size: 635 6488 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PmlI CAC'GTG Cuts at: 2155 2599 3379 2155 Size: 444 780 5899 Fragments arranged by size: 5899 780 444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4220 4222 4252 5192 5224 6705 6737 4190 Size: 30 2 30 940 32 1481 32 4576 Fragments arranged by size: 4576 1481 940 32 32 30 30 2 PpuMI rG'GwC_Cy Cuts at: 2689 3019 4023 4132 2689 Size: 330 1004 109 5680 Fragments arranged by size: 5680 1004 330 109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7123 PsiI TTA'TAA Cuts at: 4693 4693 Size: 7123 PstI C_TGCA'G Cuts at: 830 2172 2555 4380 4475 830 Size: 1342 383 1825 95 3478 Fragments arranged by size: 3478 1825 1342 383 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 PvuII CAG'CTG Cuts at: 4317 4317 Size: 7123 SacI G_AGCT'C Cuts at: 2313 2313 Size: 7123 SacII CC_GC'GG Cuts at: 3522 3522 Size: 7123 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SanDI GG'GwC_CC Cuts at: 4023 4023 Size: 7123 SapI GCTCTTCn'nnn_ Cuts at: 2927 4403 2927 Size: 1476 5647 SbfI CC_TGCA'GG Cuts at: 4475 4475 Size: 7123 ScaI AGT'ACT Cuts at: 5371 7083 5371 Size: 1712 5411 SfcI C'TryA_G Cuts at: 826 1045 2168 2182 2551 4063 4376 4471 5606 Size: 219 1123 14 369 1512 313 95 1135 Cuts at: 5606 6287 6478 826 Size: 681 191 1471 Fragments arranged by size: 1512 1471 1135 1123 681 369 313 219 191 95 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7123 SmaI CCC'GGG Cuts at: 1872 2540 3309 4450 1872 Size: 668 769 1141 4545 Fragments arranged by size: 4545 1141 769 668 SmlI C'TyrA_G Cuts at: 820 1017 1943 4145 5227 6128 6390 820 Size: 197 926 2202 1082 901 262 1553 Fragments arranged by size: 2202 1553 1082 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4914 493 Size: 4421 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 4481 1132 Size: 3349 3774 SspI AAT'ATT Cuts at: 5 52 5047 5 Size: 47 4995 2081 Fragments arranged by size: 4995 2081 47 StuI AGG'CCT Cuts at: 1517 2166 1517 Size: 649 6474 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2112 2292 2739 2907 3264 Size: 552 939 60 48 180 447 168 357 Cuts at: 3264 3457 3615 3809 4050 4562 513 Size: 193 158 194 241 512 3074 Fragments arranged by size: 3074 939 552 512 447 357 241 194 193 180 168 158 60 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2384 5150 5167 5320 5505 5950 325 Size: 1255 804 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 5369 7081 Size: 274 80 33 51 1443 468 2924 1712 Cuts at: 7081 96 Size: 138 Fragments arranged by size: 2924 1712 1443 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7083 7115 7083 Size: 32 7091 Tth111I GACn'n_nGTC Cuts at: 1316 2870 1316 Size: 1554 5569 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2254 2572 2821 3562 2254 Size: 318 249 741 5815 Fragments arranged by size: 5815 741 318 249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7123 XmaI C'CCGG_G Cuts at: 1870 2538 3307 4448 1870 Size: 668 769 1141 4545 Fragments arranged by size: 4545 1141 769 668 XmnI GAAnn'nnTTC Cuts at: 3467 5252 3467 Size: 1785 5338 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2587 2653 4931 276 Size: 53 83 186 936 1053 66 2278 2468 Fragments arranged by size: 2468 2278 1053 936 186 83 66 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI