(Linear) (Six-base) MAPSORT of: KIBB6293.seq Check: 3531 from: 1 to: 2418 KIBB6293.orf With 182 enzymes: SgfI * July 3, 2012 16:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 492 2346 2418 Size: 492 1854 72 Fragments arranged by size: 1854 492 72 AatII G_ACGT'C Cuts at: 0 584 650 2418 Size: 584 66 1768 Fragments arranged by size: 1768 584 66 AflIII A'CryG_T Cuts at: 0 413 2264 2418 Size: 413 1851 154 Fragments arranged by size: 1851 413 154 AhdI GACnn_n'nnGTC Cuts at: 0 93 1620 2418 Size: 93 1527 798 Fragments arranged by size: 1527 798 93 AleI CACnn'nnGTG Cuts at: 0 2248 2418 Size: 2248 170 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2185 2215 2217 2247 2418 Size: 2185 30 2 30 171 Fragments arranged by size: 2185 171 30 30 2 AlwNI CAG_nnn'CTG Cuts at: 0 141 171 252 687 974 2226 2370 2418 Size: 141 30 81 435 287 1252 144 48 Fragments arranged by size: 1252 435 287 144 141 81 48 30 ApoI r'AATT_y Cuts at: 0 1458 2418 Size: 1458 960 AvaI C'yCGr_G Cuts at: 0 467 533 1302 1735 2004 2418 Size: 467 66 769 433 269 414 Fragments arranged by size: 769 467 433 414 269 66 BamHI G'GATC_C Cuts at: 0 44 1307 2418 Size: 44 1263 1111 Fragments arranged by size: 1263 1111 44 BanI G'GyrC_C Cuts at: 0 1318 2418 Size: 1318 1100 BanII G_rGCy'C Cuts at: 0 308 545 901 2057 2418 Size: 308 237 356 1156 361 Fragments arranged by size: 1156 361 356 308 237 BbsI GAAGACnn'nnnn_ Cuts at: 0 861 2418 Size: 861 1557 BbvCI CC'TCA_GC Cuts at: 0 25 121 1084 1688 2418 Size: 25 96 963 604 730 Fragments arranged by size: 963 730 604 96 25 BciVI GTATCCnnnnn_n' Cuts at: 0 1868 2418 Size: 1868 550 BclI T'GATC_A Cuts at: 0 1530 2418 Size: 1530 888 BglII A'GATC_T Cuts at: 0 764 1491 2418 Size: 764 727 927 Fragments arranged by size: 927 764 727 Bme1580I G_kGCm'C Cuts at: 0 1321 2301 2418 Size: 1321 980 117 Fragments arranged by size: 1321 980 117 BmgBI CAC'GTC Cuts at: 0 557 963 1446 2418 Size: 557 406 483 972 Fragments arranged by size: 972 557 483 406 BmrI ACTGGGnnnn_n' Cuts at: 0 1236 2418 Size: 1236 1182 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 315 2418 Size: 315 2103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 624 709 753 2418 Size: 592 32 85 44 1665 Fragments arranged by size: 1665 592 85 44 32 Bpu10I CC'TnA_GC Cuts at: 0 25 121 1084 1688 2418 Size: 25 96 963 604 730 Fragments arranged by size: 963 730 604 96 25 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2125 2418 Size: 2125 293 BsaI GGTCTCn'nnnn_ Cuts at: 0 775 2418 Size: 775 1643 BsaAI yAC'GTr Cuts at: 0 150 594 1374 2418 Size: 150 444 780 1044 Fragments arranged by size: 1044 780 444 150 BsaHI Gr'CG_yC Cuts at: 0 581 647 2134 2418 Size: 581 66 1487 284 Fragments arranged by size: 1487 581 284 66 BsaWI w'CCGG_w Cuts at: 0 1826 2418 Size: 1826 592 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 304 334 343 373 2185 2215 2245 2418 Size: 304 30 9 30 1812 30 30 173 Fragments arranged by size: 1812 304 173 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 589 1936 1939 1942 2209 2418 Size: 130 459 1347 3 3 267 209 Fragments arranged by size: 1347 459 267 209 130 3 3 BseYI C'CCAG_C Cuts at: 0 819 1096 1839 2072 2418 Size: 819 277 743 233 346 Fragments arranged by size: 819 743 346 277 233 BsiEI CG_ry'CG Cuts at: 0 380 886 1392 1624 2152 2249 2418 Size: 380 506 506 232 528 97 169 Fragments arranged by size: 528 506 506 380 232 169 97 BsiHKAI G_wGCw'C Cuts at: 0 275 308 2418 Size: 275 33 2110 Fragments arranged by size: 2110 275 33 BsmBI CGTCTCn'nnnn_ Cuts at: 0 418 915 2191 2418 Size: 418 497 1276 227 Fragments arranged by size: 1276 497 418 227 Bsp1286I G_dGCh'C Cuts at: 0 275 308 545 901 1321 2057 2301 2418 Size: 275 33 237 356 420 736 244 117 Fragments arranged by size: 736 420 356 275 244 237 117 33 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 492 2346 2418 Size: 492 1854 72 Fragments arranged by size: 1854 492 72 BsrBI CCG'CTC Cuts at: 0 294 741 882 932 1164 2159 2418 Size: 294 447 141 50 232 995 259 Fragments arranged by size: 995 447 294 259 232 141 50 BsrDI GCAATG_nn' Cuts at: 0 1656 2415 2418 Size: 1656 759 3 Fragments arranged by size: 1656 759 3 BsrFI r'CCGG_y Cuts at: 0 564 1007 1632 2147 2151 2418 Size: 564 443 625 515 4 267 Fragments arranged by size: 625 564 515 443 267 4 BsrGI T'GTAC_A Cuts at: 0 440 2418 Size: 440 1978 BssHII G'CGCG_C Cuts at: 0 655 657 2418 Size: 655 2 1761 Fragments arranged by size: 1761 655 2 BssSI C'ACGA_G Cuts at: 0 268 1759 2418 Size: 268 1491 659 Fragments arranged by size: 1491 659 268 BstAPI GCAn_nnn'nTGC Cuts at: 0 950 2276 2418 Size: 950 1326 142 Fragments arranged by size: 1326 950 142 BstXI CCAn_nnnn'nTGG Cuts at: 0 1146 1378 2418 Size: 1146 232 1040 Fragments arranged by size: 1146 1040 232 BstYI r'GATC_y Cuts at: 0 44 131 764 1307 1491 2287 2418 Size: 44 87 633 543 184 796 131 Fragments arranged by size: 796 633 543 184 131 87 44 BtgI C'CryG_G Cuts at: 0 59 366 902 1313 1452 1514 2418 Size: 59 307 536 411 139 62 904 Fragments arranged by size: 904 536 411 307 139 62 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1052 2418 Size: 1052 1366 BtsI GCAGTG_nn' Cuts at: 0 948 1095 2418 Size: 948 147 1323 Fragments arranged by size: 1323 948 147 DraIII CAC_nnn'GTG Cuts at: 0 41 84 1325 2265 2418 Size: 41 43 1241 940 153 Fragments arranged by size: 1241 940 153 43 41 DrdI GACnn_nn'nnGTC Cuts at: 0 223 2418 Size: 223 2195 EaeI y'GGCC_r Cuts at: 0 201 377 883 1202 1389 1883 2149 2412 Size: 201 176 506 319 187 494 266 263 Cuts at: 2412 2418 Size: 6 Fragments arranged by size: 506 494 319 266 263 201 187 176 6 EagI C'GGCC_G Cuts at: 0 377 883 1389 2149 2418 Size: 377 506 506 760 269 Fragments arranged by size: 760 506 506 377 269 EarI CTCTTCn'nnn_ Cuts at: 0 217 922 2398 2418 Size: 217 705 1476 20 Fragments arranged by size: 1476 705 217 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 141 217 247 2418 Size: 141 76 30 2171 Fragments arranged by size: 2171 141 76 30 EcoICRI GAG'CTC Cuts at: 0 306 2418 Size: 306 2112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 624 709 753 2418 Size: 592 32 85 44 1665 Fragments arranged by size: 1665 592 85 44 32 EcoO109I rG'GnC_Cy Cuts at: 0 525 684 1014 2018 2127 2418 Size: 525 159 330 1004 109 291 Fragments arranged by size: 1004 525 330 291 159 109 FspI TGC'GCA Cuts at: 0 1648 2418 Size: 1648 770 HaeII r_GCGC'y Cuts at: 0 571 1561 1770 2418 Size: 571 990 209 648 Fragments arranged by size: 990 648 571 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 79 111 304 336 343 375 1328 1360 Size: 79 32 193 32 7 32 953 32 Cuts at: 1360 1606 1638 2418 Size: 246 32 780 Fragments arranged by size: 953 780 246 193 79 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 0 909 2418 Size: 909 1509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 56 335 826 1307 2418 Size: 56 279 491 481 1111 Fragments arranged by size: 1111 491 481 279 56 MscI TGG'CCA Cuts at: 0 1885 2418 Size: 1885 533 MslI CAynn'nnrTG Cuts at: 0 478 856 2248 2356 2418 Size: 478 378 1392 108 62 Fragments arranged by size: 1392 478 378 108 62 MspA1I CmG'CkG Cuts at: 0 1221 1516 1687 2312 2418 Size: 1221 295 171 625 106 Fragments arranged by size: 1221 625 295 171 106 NaeI GCC'GGC Cuts at: 0 566 1009 2153 2418 Size: 566 443 1144 265 Fragments arranged by size: 1144 566 443 265 NcoI C'CATG_G Cuts at: 0 59 902 1452 2418 Size: 59 843 550 966 Fragments arranged by size: 966 843 550 59 NgoMIV G'CCGG_C Cuts at: 0 564 1007 2151 2418 Size: 564 443 1144 267 Fragments arranged by size: 1144 564 443 267 NotI GC'GGCC_GC Cuts at: 0 377 2418 Size: 377 2041 NspI r_CATG'y Cuts at: 0 417 2418 Size: 417 2001 PciI A'CATG_T Cuts at: 0 413 2418 Size: 413 2005 PflMI CCAn_nnn'nTGG Cuts at: 0 84 252 1616 2117 2418 Size: 84 168 1364 501 301 Fragments arranged by size: 1364 501 301 168 84 PfoI T'CCnGG_A Cuts at: 0 134 769 2418 Size: 134 635 1649 Fragments arranged by size: 1649 635 134 PmlI CAC'GTG Cuts at: 0 150 594 1374 2418 Size: 150 444 780 1044 Fragments arranged by size: 1044 780 444 150 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2185 2215 2217 2247 2418 Size: 2185 30 2 30 171 Fragments arranged by size: 2185 171 30 30 2 PpuMI rG'GwC_Cy Cuts at: 0 684 1014 2018 2127 2418 Size: 684 330 1004 109 291 Fragments arranged by size: 1004 684 330 291 109 PstI C_TGCA'G Cuts at: 0 167 550 2375 2418 Size: 167 383 1825 43 Fragments arranged by size: 1825 383 167 43 PvuII CAG'CTG Cuts at: 0 2312 2418 Size: 2312 106 SacI G_AGCT'C Cuts at: 0 308 2418 Size: 308 2110 SacII CC_GC'GG Cuts at: 0 1517 2418 Size: 1517 901 SanDI GG'GwC_CC Cuts at: 0 2018 2418 Size: 2018 400 SapI GCTCTTCn'nnn_ Cuts at: 0 922 2398 2418 Size: 922 1476 20 Fragments arranged by size: 1476 922 20 SfcI C'TryA_G Cuts at: 0 163 177 546 2058 2371 2418 Size: 163 14 369 1512 313 47 Fragments arranged by size: 1512 369 313 163 47 14 SmaI CCC'GGG Cuts at: 0 535 1304 2418 Size: 535 769 1114 Fragments arranged by size: 1114 769 535 SmlI C'TyrA_G Cuts at: 0 2140 2418 Size: 2140 278 StuI AGG'CCT Cuts at: 0 161 2418 Size: 161 2257 StyI C'CwwG_G Cuts at: 0 59 107 287 734 902 1259 1452 1610 Size: 59 48 180 447 168 357 193 158 Cuts at: 1610 1804 2045 2418 Size: 194 241 373 Fragments arranged by size: 447 373 357 241 194 193 180 168 158 59 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 379 2418 Size: 379 2039 TatI w'GTAC_w Cuts at: 0 440 2418 Size: 440 1978 Tth111I GACn'n_nGTC Cuts at: 0 865 2418 Size: 865 1553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 249 567 816 1557 2418 Size: 249 318 249 741 861 Fragments arranged by size: 861 741 318 249 249 XmaI C'CCGG_G Cuts at: 0 533 1302 2418 Size: 533 769 1116 Fragments arranged by size: 1116 769 533 XmnI GAAnn'nnTTC Cuts at: 0 1462 2418 Size: 1462 956 ZraI GAC'GTC Cuts at: 0 582 648 2418 Size: 582 66 1770 Fragments arranged by size: 1770 582 66 Enzymes that do cut: AarI AatII AflIII AhdI AleI AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PciI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII SanDI SapI SfcI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AfeI AflII AgeI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BglI BlpI BmtI BsaBI BsgI BsiWI BsmI BspEI BspHI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NruI NsiI PacI PasI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI