(Circular) (Six-base) MAPSORT of: pFN21AE1172.seq Check: 3680 from: 1 to: 6589 pFN21AE1172 6589 bp With 182 enzymes: SgfI * March 28, 2012 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4399 278 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3932 1937 Size: 1995 4594 Acc65I G'GTAC_C Cuts at: 3910 3910 Size: 6589 AclI AA'CG_TT Cuts at: 1563 4716 5089 1563 Size: 3153 373 3063 Fragments arranged by size: 3153 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2737 3409 3493 3829 4652 6036 6304 1632 Size: 1105 672 84 336 823 1384 268 1917 Fragments arranged by size: 1917 1384 1105 823 672 336 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6392 AflIII A'CryG_T Cuts at: 1203 5488 6465 1203 Size: 4285 977 1327 Fragments arranged by size: 4285 1327 977 AgeI A'CCGG_T Cuts at: 5420 5420 Size: 6589 AhdI GACnn_n'nnGTC Cuts at: 5318 5318 Size: 6589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6589 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6589 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 2123 Size: 32 6557 AlwNI CAG_nnn'CTG Cuts at: 4332 5904 4332 Size: 1572 5017 ApaLI G'TGCA_C Cuts at: 4646 5802 4646 Size: 1156 5433 ApoI r'AATT_y Cuts at: 1493 2030 2388 2454 2787 2871 2955 3207 3459 Size: 537 358 66 333 84 84 252 252 Cuts at: 3459 3543 3795 3898 4124 4276 6385 1493 Size: 84 252 103 226 152 2109 1697 Fragments arranged by size: 2109 1697 537 358 333 252 252 252 226 152 103 84 84 84 66 AseI AT'TA_AT Cuts at: 160 1032 5143 160 Size: 872 4111 1606 Fragments arranged by size: 4111 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 AvaI C'yCGr_G Cuts at: 1870 1943 3914 1870 Size: 73 1971 4545 Fragments arranged by size: 4545 1971 73 BamHI G'GATC_C Cuts at: 3919 4286 3919 Size: 367 6222 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3910 5365 618 Size: 325 217 627 2123 1455 1842 Fragments arranged by size: 2123 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2285 3908 1391 Size: 894 1623 4072 Fragments arranged by size: 4072 1623 894 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2147 2911 3415 3429 3499 3765 3835 Size: 971 248 764 504 14 70 266 70 Cuts at: 3835 928 Size: 3682 Fragments arranged by size: 3682 971 764 504 266 248 70 70 14 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4780 4814 1903 Size: 34 2843 34 3678 Fragments arranged by size: 3678 2843 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4481 5691 4481 Size: 1210 5379 BclI T'GATC_A Cuts at: 1548 2506 1548 Size: 958 5631 BfrBI ATG'CAT Cuts at: 2707 2707 Size: 6589 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5200 136 Size: 107 122 71 631 605 3528 1525 Fragments arranged by size: 3528 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3252 3756 6584 3252 Size: 504 2828 3257 Fragments arranged by size: 3257 2828 504 BlpI GC'TnA_GC Cuts at: 4006 4006 Size: 6589 Bme1580I G_kGCm'C Cuts at: 1790 4650 5806 1790 Size: 2860 1156 2573 Fragments arranged by size: 2860 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4320 5278 442 Size: 416 53 83 399 24 2903 958 1753 Fragments arranged by size: 2903 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6589 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2299 2299 Size: 6589 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2733 2817 2901 2985 3489 3657 5249 1239 Size: 1494 84 84 84 504 168 1592 2579 Fragments arranged by size: 2579 1592 1494 504 168 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4714 5579 5877 4714 Size: 865 298 5426 Fragments arranged by size: 5426 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 3217 5252 882 Size: 617 644 1074 2035 2219 Fragments arranged by size: 2219 2035 1074 644 617 BsaAI yAC'GTr Cuts at: 493 1204 4380 493 Size: 711 3176 2702 Fragments arranged by size: 3176 2702 711 BsaBI GATnn'nnATC Cuts at: 6583 6583 Size: 6589 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4396 4778 5413 275 Size: 53 83 186 936 2863 382 635 1451 Fragments arranged by size: 2863 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5022 5420 5694 5841 6252 5022 Size: 398 274 147 411 5359 Fragments arranged by size: 5359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2123 2153 1178 Size: 30 165 30 720 30 5614 Fragments arranged by size: 5614 720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2293 1186 Size: 1107 5482 BseYI C'CCAG_C Cuts at: 5792 6288 5792 Size: 496 6093 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3853 1163 Size: 573 2117 3899 Fragments arranged by size: 3899 2117 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4039 4800 5828 1540 Size: 275 186 2038 761 1028 2301 Fragments arranged by size: 2301 2038 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2285 2577 4650 4735 5806 2285 Size: 292 2073 85 1071 3068 Fragments arranged by size: 3068 2073 1071 292 85 BsmI GAATG_Cn' Cuts at: 2709 4100 4193 2709 Size: 1391 93 5105 Fragments arranged by size: 5105 1391 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2285 2577 3908 4650 4735 5806 1391 Size: 399 495 292 1331 742 85 1071 2174 Fragments arranged by size: 2174 1331 1071 742 495 399 292 85 BspEI T'CCGG_A Cuts at: 6252 6252 Size: 6589 BspHI T'CATG_A Cuts at: 2734 2818 2902 3733 4478 6208 2734 Size: 84 84 831 745 1730 3115 Fragments arranged by size: 3115 1730 831 745 84 84 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3944 844 Size: 3100 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4476 1394 Size: 429 2653 3507 Fragments arranged by size: 3507 2653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5084 5258 65 Size: 1183 3836 174 1396 Fragments arranged by size: 3836 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5233 5420 1700 Size: 110 3423 187 2869 Fragments arranged by size: 3423 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6589 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6589 BssSI C'ACGA_G Cuts at: 4649 5661 4649 Size: 1012 5577 BstAPI GCAn_nnn'nTGC Cuts at: 794 2253 3831 794 Size: 1459 1578 3552 Fragments arranged by size: 3552 1578 1459 BstBI TT'CG_AA Cuts at: 2839 5393 2839 Size: 2554 4035 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6589 BstYI r'GATC_y Cuts at: 1972 2197 3252 3756 3919 4286 4671 4688 6140 Size: 225 1055 504 163 367 385 17 1452 Cuts at: 6140 6226 6238 6584 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1055 504 385 367 346 225 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5850 Fragments arranged by size: 5850 683 56 BtsI GCAGTG_nn' Cuts at: 2656 4105 4899 4919 2656 Size: 1449 794 20 4326 Fragments arranged by size: 4326 1449 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6589 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6554 DraI TTT'AAA Cuts at: 2484 3893 2484 Size: 1409 5180 DraIII CAC_nnn'GTG Cuts at: 3831 3831 Size: 6589 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5596 809 Size: 507 4280 1802 Fragments arranged by size: 4280 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2578 4036 Size: 54 1425 50 275 18 213 535 1458 Cuts at: 4036 4925 6554 8 Size: 889 1629 43 Fragments arranged by size: 1629 1458 1425 889 535 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4036 1537 Size: 275 2224 4090 Fragments arranged by size: 4090 2224 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2058 2214 2756 4519 1353 Size: 705 156 542 1763 3423 Fragments arranged by size: 3423 1763 705 542 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5176 5550 5696 387 Size: 1064 138 3587 374 146 1280 Fragments arranged by size: 3587 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2283 2283 Size: 6589 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2733 2737 2817 2901 2985 3409 3489 Size: 393 1101 4 80 84 84 424 80 Cuts at: 3489 3493 3657 3829 4652 5249 6036 6304 1239 Size: 4 164 172 823 597 787 268 1524 Fragments arranged by size: 1524 1101 823 787 597 424 393 268 172 164 84 84 80 80 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2015 Size: 6589 EcoO109I rG'GnC_Cy Cuts at: 2021 2021 Size: 6589 EcoRI G'AATT_C Cuts at: 2787 2871 3207 3459 3795 3898 4276 2787 Size: 84 336 252 336 103 378 5100 Fragments arranged by size: 5100 378 336 336 252 103 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3159 3159 Size: 6589 FspI TGC'GCA Cuts at: 2088 5095 2088 Size: 3007 3582 HaeII r_GCGC'y Cuts at: 1678 5736 1678 Size: 4058 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 3184 3216 3244 Size: 32 135 32 845 32 735 32 28 Cuts at: 3244 3276 3748 3780 5230 5262 5304 5336 1373 Size: 32 472 32 1450 32 42 32 2626 Fragments arranged by size: 2626 1450 845 735 472 135 42 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3933 4179 669 Size: 988 281 1995 246 3079 Fragments arranged by size: 3079 1995 988 281 246 HindIII A'AGCT_T Cuts at: 748 2556 2684 2852 748 Size: 1808 128 168 4485 Fragments arranged by size: 4485 1808 168 128 HpaI GTT'AAC Cuts at: 4179 4179 Size: 6589 KpnI G_GTAC'C Cuts at: 3914 3914 Size: 6589 MfeI C'AATT_G Cuts at: 4188 4188 Size: 6589 MluI A'CGCG_T Cuts at: 6465 6465 Size: 6589 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 5703 5887 1305 Size: 792 3606 184 2007 Fragments arranged by size: 3606 2007 792 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 4554 Fragments arranged by size: 4554 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2355 2739 2823 2907 3048 3300 Size: 648 873 316 384 84 84 141 252 Cuts at: 3300 4547 4906 5065 518 Size: 1247 359 159 2042 Fragments arranged by size: 2042 1247 873 648 384 359 316 252 159 141 84 84 MspA1I CmG'CkG Cuts at: 1606 4005 4682 5830 6075 1606 Size: 2399 677 1148 245 2120 Fragments arranged by size: 2399 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5098 Fragments arranged by size: 5098 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6589 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6589 NotI GC'GGCC_GC Cuts at: 4036 4036 Size: 6589 NsiI A_TGCA'T Cuts at: 2709 2709 Size: 6589 NspI r_CATG'y Cuts at: 1132 1229 3947 5492 1132 Size: 97 2718 1545 2229 Fragments arranged by size: 2718 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2143 1348 Size: 795 5794 PciI A'CATG_T Cuts at: 5488 5488 Size: 6589 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6589 PmeI GTTT'AAAC Cuts at: 3893 3893 Size: 6589 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 2155 4658 4690 6171 6203 2123 Size: 32 2503 32 1481 32 2509 Fragments arranged by size: 2509 2503 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2021 2021 Size: 6589 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6589 PsiI TTA'TAA Cuts at: 2562 4159 2562 Size: 1597 4992 PstI C_TGCA'G Cuts at: 830 2214 3941 830 Size: 1384 1727 3478 Fragments arranged by size: 3478 1727 1384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6589 SacI G_AGCT'C Cuts at: 2285 2285 Size: 6589 SalI G'TCGA_C Cuts at: 1936 3931 1936 Size: 1995 4594 SanDI GG'GwC_CC Cuts at: 2021 2021 Size: 6589 SapI GCTCTTCn'nnn_ Cuts at: 2756 2756 Size: 6589 SbfI CC_TGCA'GG Cuts at: 3941 3941 Size: 6589 ScaI AGT'ACT Cuts at: 4837 6549 4837 Size: 1712 4877 SexAI A'CCwGG_T Cuts at: 2133 3525 2133 Size: 1392 5197 SfcI C'TryA_G Cuts at: 826 1045 2210 2363 3937 5072 5753 5944 826 Size: 219 1165 153 1574 1135 681 191 1471 Fragments arranged by size: 1574 1471 1165 1135 681 219 191 153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6589 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6589 SmaI CCC'GGG Cuts at: 1872 3916 1872 Size: 2044 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4693 5594 5856 820 Size: 197 926 2750 901 262 1553 Fragments arranged by size: 2750 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4380 493 Size: 3887 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6589 SphI G_CATG'C Cuts at: 1132 3947 1132 Size: 2815 3774 SspI AAT'ATT Cuts at: 5 52 4513 5 Size: 47 4461 2081 Fragments arranged by size: 4461 2081 47 StuI AGG'CCT Cuts at: 1517 3646 3693 1517 Size: 2129 47 4413 Fragments arranged by size: 4413 2129 47 StyI C'CwwG_G Cuts at: 513 1065 2004 4028 513 Size: 552 939 2024 3074 Fragments arranged by size: 3074 2024 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2385 4616 4633 4786 4971 5416 325 Size: 1255 805 2231 17 153 185 445 1498 Fragments arranged by size: 2231 1498 1255 805 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4835 6547 96 Size: 274 80 33 51 1443 2858 1712 138 Fragments arranged by size: 2858 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6549 6581 6549 Size: 32 6557 Tth111I GACn'n_nGTC Cuts at: 1316 2831 2915 3167 1316 Size: 1515 84 252 4738 Fragments arranged by size: 4738 1515 252 84 XbaI T'CTAG_A Cuts at: 3925 3925 Size: 6589 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6589 XmaI C'CCGG_G Cuts at: 1870 3914 1870 Size: 2044 4545 XmnI GAAnn'nnTTC Cuts at: 2845 4718 2845 Size: 1873 4716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4397 276 Size: 53 83 186 936 2863 2468 Fragments arranged by size: 2863 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI XcmI