(Linear) (Six-base) MAPSORT of: KIEE1172.seq Check: 4214 from: 1 to: 1884 KIEE1172.orf With 182 enzymes: SgfI * March 22, 2012 15:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 732 1404 1488 1824 1884 Size: 732 672 84 336 60 Fragments arranged by size: 732 672 336 84 60 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 118 150 1884 Size: 118 32 1734 Fragments arranged by size: 1734 118 32 ApoI r'AATT_y Cuts at: 0 25 383 449 782 866 950 1202 1454 Size: 25 358 66 333 84 84 252 252 Cuts at: 1454 1538 1790 1884 Size: 84 252 94 Fragments arranged by size: 358 333 252 252 252 94 84 84 84 66 25 BanII G_rGCy'C Cuts at: 0 280 1884 Size: 280 1604 BbsI GAAGACnn'nnnn_ Cuts at: 0 142 906 1410 1424 1494 1760 1830 1884 Size: 142 764 504 14 70 266 70 54 Fragments arranged by size: 764 504 266 142 70 70 54 14 BclI T'GATC_A Cuts at: 0 501 1884 Size: 501 1383 BfrBI ATG'CAT Cuts at: 0 702 1884 Size: 702 1182 BglII A'GATC_T Cuts at: 0 1247 1751 1884 Size: 1247 504 133 Fragments arranged by size: 1247 504 133 BmgBI CAC'GTC Cuts at: 0 105 1884 Size: 105 1779 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 294 1884 Size: 294 1590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 728 812 896 980 1484 1652 1884 Size: 728 84 84 84 504 168 232 Fragments arranged by size: 728 504 232 168 84 84 84 BsaI GGTCTCn'nnnn_ Cuts at: 0 138 1212 1884 Size: 138 1074 672 Fragments arranged by size: 1074 672 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 118 148 1884 Size: 118 30 1736 Fragments arranged by size: 1736 118 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 288 1884 Size: 288 1596 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1848 1884 Size: 1848 36 BsiHKAI G_wGCw'C Cuts at: 0 280 572 1884 Size: 280 292 1312 Fragments arranged by size: 1312 292 280 BsmI GAATG_Cn' Cuts at: 0 704 1884 Size: 704 1180 Bsp1286I G_dGCh'C Cuts at: 0 280 572 1884 Size: 280 292 1312 Fragments arranged by size: 1312 292 280 BspHI T'CATG_A Cuts at: 0 729 813 897 1728 1884 Size: 729 84 84 831 156 Fragments arranged by size: 831 729 156 84 84 BstAPI GCAn_nnn'nTGC Cuts at: 0 248 1826 1884 Size: 248 1578 58 Fragments arranged by size: 1578 248 58 BstBI TT'CG_AA Cuts at: 0 834 1884 Size: 834 1050 BstYI r'GATC_y Cuts at: 0 192 1247 1751 1884 Size: 192 1055 504 133 Fragments arranged by size: 1055 504 192 133 BtsI GCAGTG_nn' Cuts at: 0 651 1884 Size: 651 1233 DraI TTT'AAA Cuts at: 0 479 1884 Size: 479 1405 DraIII CAC_nnn'GTG Cuts at: 0 1826 1884 Size: 1826 58 EaeI y'GGCC_r Cuts at: 0 38 573 1884 Size: 38 535 1311 Fragments arranged by size: 1311 535 38 EarI CTCTTCn'nnn_ Cuts at: 0 53 209 751 1884 Size: 53 156 542 1133 Fragments arranged by size: 1133 542 156 53 EcoICRI GAG'CTC Cuts at: 0 278 1884 Size: 278 1606 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 728 732 812 896 980 1404 1484 1488 Size: 728 4 80 84 84 424 80 4 Cuts at: 1488 1652 1824 1884 Size: 164 172 60 Fragments arranged by size: 728 424 172 164 84 84 80 80 60 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 10 1884 Size: 10 1874 EcoO109I rG'GnC_Cy Cuts at: 0 16 1884 Size: 16 1868 EcoRI G'AATT_C Cuts at: 0 782 866 1202 1454 1790 1884 Size: 782 84 336 252 336 94 Fragments arranged by size: 782 336 336 252 94 84 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1154 1884 Size: 1154 730 FspI TGC'GCA Cuts at: 0 83 1884 Size: 83 1801 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 412 444 1179 1211 1239 1271 1743 1775 Size: 412 32 735 32 28 32 472 32 Cuts at: 1775 1884 Size: 109 Fragments arranged by size: 735 472 412 109 32 32 32 32 28 HindIII A'AGCT_T Cuts at: 0 551 679 847 1884 Size: 551 128 168 1037 Fragments arranged by size: 1037 551 168 128 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 92 1884 Size: 92 1792 MscI TGG'CCA Cuts at: 0 40 1884 Size: 40 1844 MslI CAynn'nnrTG Cuts at: 0 34 350 734 818 902 1043 1295 1884 Size: 34 316 384 84 84 141 252 589 Fragments arranged by size: 589 384 316 252 141 84 84 34 NsiI A_TGCA'T Cuts at: 0 704 1884 Size: 704 1180 PasI CC'CwG_GG Cuts at: 0 138 1884 Size: 138 1746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 118 150 1884 Size: 118 32 1734 Fragments arranged by size: 1734 118 32 PpuMI rG'GwC_Cy Cuts at: 0 16 1884 Size: 16 1868 PsiI TTA'TAA Cuts at: 0 557 1884 Size: 557 1327 PstI C_TGCA'G Cuts at: 0 209 1884 Size: 209 1675 SacI G_AGCT'C Cuts at: 0 280 1884 Size: 280 1604 SanDI GG'GwC_CC Cuts at: 0 16 1884 Size: 16 1868 SapI GCTCTTCn'nnn_ Cuts at: 0 751 1884 Size: 751 1133 SexAI A'CCwGG_T Cuts at: 0 128 1520 1884 Size: 128 1392 364 Fragments arranged by size: 1392 364 128 SfcI C'TryA_G Cuts at: 0 205 358 1884 Size: 205 153 1526 Fragments arranged by size: 1526 205 153 StuI AGG'CCT Cuts at: 0 1641 1688 1884 Size: 1641 47 196 Fragments arranged by size: 1641 196 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 380 1884 Size: 380 1504 Tth111I GACn'n_nGTC Cuts at: 0 826 910 1162 1884 Size: 826 84 252 722 Fragments arranged by size: 826 722 252 84 XmnI GAAnn'nnTTC Cuts at: 0 840 1884 Size: 840 1044 Enzymes that do cut: AcuI AloI ApoI BanII BbsI BclI BfrBI BglII BmgBI BplI BpmI BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BstAPI BstBI BstYI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HindIII MmeI MscI MslI NsiI PasI PpiI PpuMI PsiI PstI SacI SanDI SapI SexAI SfcI StuI TaqII Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmrI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI EcoRV FseI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PciI PflMI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI XbaI XcmI XhoI XmaI ZraI