Restriction map for
pFN21AE2470
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MaeIII MamI MfeI
MluNI MroI MscI MslI MunI
NdeI NheI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SfcI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tsp45I Tsp509I Van91I
XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 886 GTCTAC GT^[AC][GT]AC
AflIII 1130 ACGCGT A^C[GA][CT]GT
AlwI 315 GATCC GGATC(4/5)
AlwNI 686 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 54 GAGCTC G[AT]GC[AT]^C
AspI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1290 GGACC G^G[AT]CC
BbsI 1204 GAAGAC GAAGAC(2/6)
BglII 263 AGATCT A^GATCT
BpmI 420 CTCCAG CTGGAG(16/14)
BpuAI 1204 GAAGAC GAAGAC(2/6)
BsiHKAI 54 GAGCTC G[AT]GC[AT]^C
Bsp1407I 776 TGTACA T^GTACA
BspMI 1211 ACCTGC ACCTGC(4/8)
BsrGI 776 TGTACA T^GTACA
BsrI 1382 ACTGG ACTGG(1/-1)
BstAPI 685 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1201 TTCGAA TT^CGAA
BstF5I 654 CATCC GGATG(2/0)
BstXI 269 CCAAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 1015 CTGAG C^T[ACGT]AG
DraII 1289 GGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1161 CTCTTC CTCTTC(1/4)
EcoNI 969 CCTGTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1289 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 495 CCATGG C^C[AT][AT]GG
FokI 654 CATCC GGATG(9/13)
Ksp632I 1161 CTCTTC CTCTTC(1/4)
KspI 1074 CCGCGG CCGC^GG
MaeII 910 ACGT A^CGT
MluI 1130 ACGCGT A^CGCGT
MseI 1301 TTAA T^TAA
NcoI 495 CCATGG C^CATGG
NotI 1019 GCGGCCGC GC^GGCCGC
NspV 1201 TTCGAA TT^CGAA
PflFI 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1289 GGGACCT [GA]G^G[AT]CC[CT]
SacI 54 GAGCTC GAGCT^C
SacII 1074 CCGCGG CCGC^GG
SexAI 1406 ACCTGGT A^CC[AT]GGT
SfaNI 1397 GCATC GCATC(5/9)
SfuI 1201 TTCGAA TT^CGAA
SmaI 849 CCCGGG CCC^GGG
SspBI 776 TGTACA T^GTACA
StyI 495 CCATGG C^C[AT][AT]GG
TfiI 86 GAATC G^A[AT]TC
Tru9I 1301 TTAA T^TAA
TspRI 480 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 466 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 849 CCCGGG C^CCGGG
XmaI 849 CCCGGG C^CCGGG
XmnI 189 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Aor51HI 36 AGCGCT AGC^GCT
837 AGCGCT
BsgI 1347 CTGCAC GTGCAG(16/14)
4 GTGCAG
BsmAI 464 GAGAC GTCTC(1/5)
1379 GAGAC
BssHII 1092 GCGCGC G^CGCGC
1118 GCGCGC
BstYI 263 AGATCT [GA]^GATC[CT]
314 AGATCC
Eco47III 36 AGCGCT AGC^GCT
837 AGCGCT
MflI 263 AGATCT [GA]^GATC[CT]
314 AGATCC
MspA1I 686 CAGCGG C[AC]G^C[GT]G
1074 CCGCGG
NspI 248 ACATGC [GA]CATG^[CT]
1247 GCATGT
PaeR7I 76 CTCGAG C^TCGAG
1387 CTCGAG
XcmI 647 CCAAGCTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
953 CCAGCGACCCCCTGG
XhoI 76 CTCGAG C^TCGAG
1387 CTCGAG
XhoII 263 AGATCT [GA]^GATC[CT]
314 AGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MboI MboII MnlI
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfoI SmlI TaqI TseI
TthHB8I