(Circular) (Six-base) MAPSORT of: pFN21AE2470.seq Check: 7993 from: 1 to: 6127 pFN21AE2470 6127 bp With 182 enzymes: SgfI * May 21, 2013 17:11 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2892 3470 1937 Size: 955 578 4594 Fragments arranged by size: 4594 955 578 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3253 4190 5574 5842 1632 Size: 1621 937 1384 268 1917 Fragments arranged by size: 1917 1621 1384 937 268 AfeI AGC'GCT Cuts at: 2043 2844 2043 Size: 801 5326 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5930 AflIII A'CryG_T Cuts at: 1203 3135 5026 6003 1203 Size: 1932 1891 977 1327 Fragments arranged by size: 1932 1891 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 2696 3870 5442 2696 Size: 1174 1572 3381 Fragments arranged by size: 3381 1572 1174 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2081 2149 2167 2854 3178 3359 Size: 73 125 13 68 18 687 324 181 Cuts at: 3359 3379 3392 3452 1870 Size: 20 13 60 4545 Fragments arranged by size: 4545 687 324 181 125 73 68 60 20 18 13 13 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2567 2756 3448 4903 Size: 325 217 627 285 495 189 692 1455 Cuts at: 4903 618 Size: 1842 Fragments arranged by size: 1842 1455 692 627 495 325 285 217 189 BanII G_rGCy'C Cuts at: 1391 2063 3185 3318 3446 1391 Size: 672 1122 133 128 4072 Fragments arranged by size: 4072 1122 672 133 128 BbeI G_GCGC'C Cuts at: 2076 2571 2760 2076 Size: 495 189 5443 Fragments arranged by size: 5443 495 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3216 928 Size: 971 1317 3839 Fragments arranged by size: 3839 1317 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2505 2726 2822 Size: 107 122 71 631 605 833 221 96 Cuts at: 2822 3024 4738 136 Size: 202 1714 1525 Fragments arranged by size: 1714 1525 833 631 605 221 202 122 107 96 71 BglII A'GATC_T Cuts at: 2268 6122 2268 Size: 3854 2273 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2178 2915 4188 5344 1790 Size: 388 737 1273 1156 2573 Fragments arranged by size: 2573 1273 1156 737 388 BmgBI CAC'GTC Cuts at: 1318 2916 1318 Size: 1598 4529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3858 4816 442 Size: 416 53 83 399 24 2441 958 1753 Fragments arranged by size: 2441 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3337 3394 3407 3337 Size: 57 13 6057 Fragments arranged by size: 6057 57 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 4787 1239 Size: 1171 2377 2579 Fragments arranged by size: 2579 2377 1171 Bpu10I CC'TnA_GC Cuts at: 3020 3020 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2339 4252 5117 5415 2339 Size: 1913 865 298 3051 Fragments arranged by size: 3051 1913 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4790 882 Size: 617 3291 2219 Fragments arranged by size: 3291 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2568 2757 3934 Size: 53 83 186 936 540 495 189 1177 Cuts at: 3934 4316 4951 275 Size: 382 635 1451 Fragments arranged by size: 1451 1177 936 635 540 495 382 189 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2414 2438 2444 2460 Size: 30 165 30 1005 6 24 6 16 Cuts at: 2460 2490 3125 3155 1178 Size: 30 635 30 4150 Fragments arranged by size: 4150 1005 635 165 30 30 30 30 24 16 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2422 2437 2556 2809 2996 2999 3133 Size: 903 333 15 119 253 187 3 134 Cuts at: 3133 3151 3154 3163 3166 3175 1186 Size: 18 3 9 3 9 4138 Fragments arranged by size: 4138 903 333 253 187 134 119 18 15 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 2086 2281 2798 2957 3014 3233 5330 5826 2086 Size: 195 517 159 57 219 2097 496 2387 Fragments arranged by size: 2387 2097 517 496 219 195 159 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2030 3337 1163 Size: 573 294 1307 3953 Fragments arranged by size: 3953 1307 573 294 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2439 3028 3061 3292 3577 4338 Size: 275 186 438 589 33 231 285 761 Cuts at: 4338 5366 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 589 438 285 275 231 186 33 BsiHKAI G_wGCw'C Cuts at: 2063 4188 4273 5344 2063 Size: 2125 85 1071 2846 Fragments arranged by size: 2846 2125 1071 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2178 2915 3185 3318 3446 4188 Size: 399 273 115 737 270 133 128 742 Cuts at: 4188 4273 5344 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 737 399 273 270 133 128 115 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3225 3482 844 Size: 2381 257 3489 Fragments arranged by size: 3489 2381 257 BsrBI CCG'CTC Cuts at: 1394 1823 2224 2344 3024 3063 4014 1394 Size: 429 401 120 680 39 951 3507 Fragments arranged by size: 3507 951 680 429 401 120 39 BsrDI GCAATG_nn' Cuts at: 65 1248 4622 4796 65 Size: 1183 3374 174 1396 Fragments arranged by size: 3374 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2051 2075 2388 2581 3056 4771 4958 Size: 110 241 24 313 193 475 1715 187 Cuts at: 4958 1700 Size: 2869 Fragments arranged by size: 2869 1715 475 313 241 193 187 110 24 BsrGI T'GTAC_A Cuts at: 96 2781 96 Size: 2685 3442 BssHII G'CGCG_C Cuts at: 1925 3097 3123 1925 Size: 1172 26 4929 Fragments arranged by size: 4929 1172 26 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 2696 794 Size: 1902 4225 BstBI TT'CG_AA Cuts at: 3207 4931 3207 Size: 1724 4403 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2281 Size: 6127 BstYI r'GATC_y Cuts at: 1972 2268 2319 3457 3824 4209 4226 5678 5764 Size: 296 51 1138 367 385 17 1452 86 Cuts at: 5764 5776 6122 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1138 385 367 346 296 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2500 2928 3079 513 Size: 552 939 123 373 428 151 3561 Fragments arranged by size: 3561 939 552 428 373 151 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2491 3643 4437 4457 2491 Size: 1152 794 20 4161 Fragments arranged by size: 4161 1152 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2461 2496 529 Size: 35 1897 35 4160 Fragments arranged by size: 4160 1897 35 35 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2436 2497 Size: 54 1425 50 275 18 219 387 61 Cuts at: 2497 3025 3058 3574 4463 6092 8 Size: 528 33 516 889 1629 43 Fragments arranged by size: 1629 1425 889 528 516 387 275 219 61 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 2436 3025 3058 3574 1537 Size: 275 624 589 33 516 4090 Fragments arranged by size: 4090 624 589 516 275 33 EarI CTCTTCn'nnn_ Cuts at: 1353 3172 4057 1353 Size: 1819 885 3423 Fragments arranged by size: 3423 1819 885 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2354 2803 2885 4714 5088 5234 Size: 1064 138 765 449 82 1829 374 146 Cuts at: 5234 387 Size: 1280 Fragments arranged by size: 1829 1280 1064 765 449 374 146 138 82 EcoICRI GAG'CTC Cuts at: 2061 2061 Size: 6127 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 3253 4190 4787 5574 5842 1239 Size: 393 778 843 937 597 787 268 1524 Fragments arranged by size: 1524 937 843 787 778 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2978 2978 Size: 6127 EcoO109I rG'GnC_Cy Cuts at: 3295 3295 Size: 6127 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2045 2076 2571 2760 2805 2846 3039 3287 Size: 367 31 495 189 45 41 193 248 Cuts at: 3287 5274 1678 Size: 1987 2531 Fragments arranged by size: 2531 1987 495 367 248 193 189 45 41 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2460 2492 2759 2791 3239 Size: 32 135 32 888 32 267 32 448 Cuts at: 3239 3271 4768 4800 4842 4874 1373 Size: 32 1497 32 42 32 2626 Fragments arranged by size: 2626 1497 888 448 267 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3717 669 Size: 988 281 1533 246 3079 Fragments arranged by size: 3079 1533 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KasI G'GCGC_C Cuts at: 2072 2567 2756 2072 Size: 495 189 5443 Fragments arranged by size: 5443 495 189 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 3135 6003 3135 Size: 2868 3259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3219 3432 5241 5425 1305 Size: 1914 213 1809 184 2007 Fragments arranged by size: 2007 1914 1809 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4305 Fragments arranged by size: 4305 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4085 4444 4603 518 Size: 648 2919 359 159 2042 Fragments arranged by size: 2919 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2693 3081 3543 4220 5368 5613 1606 Size: 1087 388 462 677 1148 245 2120 Fragments arranged by size: 2120 1148 1087 677 462 388 245 NaeI GCC'GGC Cuts at: 2053 2077 3058 2053 Size: 24 981 5122 Fragments arranged by size: 5122 981 24 NarI GG'CG_CC Cuts at: 2073 2568 2757 2073 Size: 495 189 5443 Fragments arranged by size: 5443 495 189 NcoI C'CATG_G Cuts at: 513 1065 2004 2500 513 Size: 552 939 496 4140 Fragments arranged by size: 4140 939 552 496 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NgoMIV G'CCGG_C Cuts at: 2051 2075 3056 2051 Size: 24 981 5122 Fragments arranged by size: 5122 981 24 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3025 3574 3025 Size: 549 5578 NspI r_CATG'y Cuts at: 1132 1229 2257 3256 3485 5030 1132 Size: 97 1028 999 229 1545 2229 Fragments arranged by size: 2229 1545 1028 999 229 97 PasI CC'CwG_GG Cuts at: 1348 2752 2968 3259 1348 Size: 1404 216 291 4216 Fragments arranged by size: 4216 1404 291 216 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6127 PfoI T'CCnGG_A Cuts at: 3342 3342 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3221 3253 4196 4228 5709 5741 3221 Size: 32 943 32 1481 32 3607 Fragments arranged by size: 3607 1481 943 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3295 3295 Size: 6127 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6127 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspXI vC'TCGA_Gb Cuts at: 2081 3392 2081 Size: 1311 4816 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 SacI G_AGCT'C Cuts at: 2063 2063 Size: 6127 SacII CC_GC'GG Cuts at: 3082 3082 Size: 6127 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SexAI A'CCwGG_T Cuts at: 3411 3411 Size: 6127 SfcI C'TryA_G Cuts at: 826 1045 3475 4610 5291 5482 826 Size: 219 2430 1135 681 191 1471 Fragments arranged by size: 2430 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2074 2569 2758 2074 Size: 495 189 5443 Fragments arranged by size: 5443 495 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 2856 3454 1872 Size: 984 598 4545 Fragments arranged by size: 4545 984 598 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 2354 3392 4231 5132 5394 Size: 197 926 138 273 1038 839 901 262 Cuts at: 5394 820 Size: 1553 Fragments arranged by size: 1553 1038 926 901 839 273 262 197 138 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 2500 3566 513 Size: 552 939 496 1066 3074 Fragments arranged by size: 3074 1066 939 552 496 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2781 4373 6085 Size: 274 80 33 51 1443 804 1592 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 1712 1592 1443 804 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 6087 6119 3125 Size: 32 2930 32 3133 Fragments arranged by size: 3133 2930 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2474 1316 Size: 1158 4969 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2659 2965 2659 Size: 306 5821 XhoI C'TCGA_G Cuts at: 1943 2081 3392 1943 Size: 138 1311 4678 Fragments arranged by size: 4678 1311 138 XmaI C'CCGG_G Cuts at: 1870 2854 3452 1870 Size: 984 598 4545 Fragments arranged by size: 4545 984 598 XmnI GAAnn'nnTTC Cuts at: 2198 4256 2198 Size: 2058 4069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI