(Linear) (Six-base) MAPSORT of: KIEE2470.seq Check: 6406 from: 1 to: 1422 KIEE2470.orf With 182 enzymes: SgfI * May 21, 2013 16:22 .. AccI GT'mk_AC Cuts at: 0 887 1422 Size: 887 535 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1248 1422 Size: 1248 174 AfeI AGC'GCT Cuts at: 0 38 839 1422 Size: 38 801 583 Fragments arranged by size: 801 583 38 AflIII A'CryG_T Cuts at: 0 1130 1422 Size: 1130 292 AlwNI CAG_nnn'CTG Cuts at: 0 691 1422 Size: 691 731 AvaI C'yCGr_G Cuts at: 0 63 76 144 162 849 1173 1354 1374 Size: 63 13 68 18 687 324 181 20 Cuts at: 1374 1387 1422 Size: 13 35 Fragments arranged by size: 687 324 181 68 63 35 20 18 13 13 BanI G'GyrC_C Cuts at: 0 67 562 751 1422 Size: 67 495 189 671 Fragments arranged by size: 671 495 189 67 BanII G_rGCy'C Cuts at: 0 58 1180 1313 1422 Size: 58 1122 133 109 Fragments arranged by size: 1122 133 109 58 BbeI G_GCGC'C Cuts at: 0 71 566 755 1422 Size: 71 495 189 667 Fragments arranged by size: 667 495 189 71 BbsI GAAGACnn'nnnn_ Cuts at: 0 1211 1422 Size: 1211 211 BglI GCCn_nnn'nGGC Cuts at: 0 500 721 817 1019 1422 Size: 500 221 96 202 403 Fragments arranged by size: 500 403 221 202 96 BglII A'GATC_T Cuts at: 0 263 1422 Size: 263 1159 Bme1580I G_kGCm'C Cuts at: 0 173 910 1422 Size: 173 737 512 Fragments arranged by size: 737 512 173 BmgBI CAC'GTC Cuts at: 0 911 1422 Size: 911 511 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1332 1389 1402 1422 Size: 1332 57 13 20 Fragments arranged by size: 1332 57 20 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1422 Size: 405 1017 Bpu10I CC'TnA_GC Cuts at: 0 1015 1422 Size: 1015 407 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 1422 Size: 334 1088 BsaHI Gr'CG_yC Cuts at: 0 68 563 752 1422 Size: 68 495 189 670 Fragments arranged by size: 670 495 189 68 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 403 409 433 439 455 485 1120 1150 Size: 403 6 24 6 16 30 635 30 Cuts at: 1150 1422 Size: 272 Fragments arranged by size: 635 403 272 30 30 24 16 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 84 417 432 551 804 991 994 1128 Size: 84 333 15 119 253 187 3 134 Cuts at: 1128 1146 1149 1158 1161 1170 1422 Size: 18 3 9 3 9 252 Fragments arranged by size: 333 253 252 187 134 119 84 18 15 9 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 81 276 793 952 1009 1228 1422 Size: 81 195 517 159 57 219 194 Fragments arranged by size: 517 219 195 194 159 81 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 25 1332 1422 Size: 25 1307 90 Fragments arranged by size: 1307 90 25 BsiEI CG_ry'CG Cuts at: 0 434 1023 1056 1287 1422 Size: 434 589 33 231 135 Fragments arranged by size: 589 434 231 135 33 BsiHKAI G_wGCw'C Cuts at: 0 58 1422 Size: 58 1364 Bsp1286I G_dGCh'C Cuts at: 0 58 173 910 1180 1313 1422 Size: 58 115 737 270 133 109 Fragments arranged by size: 737 270 133 115 109 58 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1220 1422 Size: 1220 202 BsrBI CCG'CTC Cuts at: 0 219 339 1019 1058 1422 Size: 219 120 680 39 364 Fragments arranged by size: 680 364 219 120 39 BsrFI r'CCGG_y Cuts at: 0 46 70 383 576 1051 1422 Size: 46 24 313 193 475 371 Fragments arranged by size: 475 371 313 193 46 24 BsrGI T'GTAC_A Cuts at: 0 776 1422 Size: 776 646 BssHII G'CGCG_C Cuts at: 0 1092 1118 1422 Size: 1092 26 304 Fragments arranged by size: 1092 304 26 BstAPI GCAn_nnn'nTGC Cuts at: 0 691 1422 Size: 691 731 BstBI TT'CG_AA Cuts at: 0 1202 1422 Size: 1202 220 BstXI CCAn_nnnn'nTGG Cuts at: 0 276 1422 Size: 276 1146 BstYI r'GATC_y Cuts at: 0 263 314 1422 Size: 263 51 1108 Fragments arranged by size: 1108 263 51 BtgI C'CryG_G Cuts at: 0 122 495 923 1074 1422 Size: 122 373 428 151 348 Fragments arranged by size: 428 373 348 151 122 BtsI GCAGTG_nn' Cuts at: 0 486 1422 Size: 486 936 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 456 491 1422 Size: 456 35 931 Fragments arranged by size: 931 456 35 EaeI y'GGCC_r Cuts at: 0 44 431 492 1020 1053 1422 Size: 44 387 61 528 33 369 Fragments arranged by size: 528 387 369 61 44 33 EagI C'GGCC_G Cuts at: 0 431 1020 1053 1422 Size: 431 589 33 369 Fragments arranged by size: 589 431 369 33 EarI CTCTTCn'nnn_ Cuts at: 0 1167 1422 Size: 1167 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 349 798 880 1422 Size: 349 449 82 542 Fragments arranged by size: 542 449 349 82 EcoICRI GAG'CTC Cuts at: 0 56 1422 Size: 56 1366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1248 1422 Size: 405 843 174 Fragments arranged by size: 843 405 174 EcoNI CCTnn'n_nnAGG Cuts at: 0 973 1422 Size: 973 449 EcoO109I rG'GnC_Cy Cuts at: 0 1290 1422 Size: 1290 132 HaeII r_GCGC'y Cuts at: 0 40 71 566 755 800 841 1034 1282 Size: 40 31 495 189 45 41 193 248 Cuts at: 1282 1422 Size: 140 Fragments arranged by size: 495 248 193 189 140 45 41 40 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 455 487 754 786 1234 1266 1422 Size: 455 32 267 32 448 32 156 Fragments arranged by size: 455 448 267 156 32 32 32 KasI G'GCGC_C Cuts at: 0 67 562 751 1422 Size: 67 495 189 671 Fragments arranged by size: 671 495 189 67 MluI A'CGCG_T Cuts at: 0 1130 1422 Size: 1130 292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1214 1422 Size: 1214 208 MspA1I CmG'CkG Cuts at: 0 688 1076 1422 Size: 688 388 346 Fragments arranged by size: 688 388 346 NaeI GCC'GGC Cuts at: 0 48 72 1053 1422 Size: 48 24 981 369 Fragments arranged by size: 981 369 48 24 NarI GG'CG_CC Cuts at: 0 68 563 752 1422 Size: 68 495 189 670 Fragments arranged by size: 670 495 189 68 NcoI C'CATG_G Cuts at: 0 495 1422 Size: 495 927 NgoMIV G'CCGG_C Cuts at: 0 46 70 1051 1422 Size: 46 24 981 371 Fragments arranged by size: 981 371 46 24 NotI GC'GGCC_GC Cuts at: 0 1020 1422 Size: 1020 402 NspI r_CATG'y Cuts at: 0 252 1251 1422 Size: 252 999 171 Fragments arranged by size: 999 252 171 PasI CC'CwG_GG Cuts at: 0 747 963 1254 1422 Size: 747 216 291 168 Fragments arranged by size: 747 291 216 168 PfoI T'CCnGG_A Cuts at: 0 1337 1422 Size: 1337 85 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1216 1248 1422 Size: 1216 32 174 Fragments arranged by size: 1216 174 32 PpuMI rG'GwC_Cy Cuts at: 0 1290 1422 Size: 1290 132 PspXI vC'TCGA_Gb Cuts at: 0 76 1387 1422 Size: 76 1311 35 Fragments arranged by size: 1311 76 35 SacI G_AGCT'C Cuts at: 0 58 1422 Size: 58 1364 SacII CC_GC'GG Cuts at: 0 1077 1422 Size: 1077 345 SexAI A'CCwGG_T Cuts at: 0 1406 1422 Size: 1406 16 SfoI GGC'GCC Cuts at: 0 69 564 753 1422 Size: 69 495 189 669 Fragments arranged by size: 669 495 189 69 SmaI CCC'GGG Cuts at: 0 851 1422 Size: 851 571 SmlI C'TyrA_G Cuts at: 0 76 349 1387 1422 Size: 76 273 1038 35 Fragments arranged by size: 1038 273 76 35 StyI C'CwwG_G Cuts at: 0 495 1422 Size: 495 927 TatI w'GTAC_w Cuts at: 0 776 1422 Size: 776 646 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1120 1152 1422 Size: 1120 32 270 Fragments arranged by size: 1120 270 32 Tth111I GACn'n_nGTC Cuts at: 0 469 1422 Size: 469 953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 654 960 1422 Size: 654 306 462 Fragments arranged by size: 654 462 306 XhoI C'TCGA_G Cuts at: 0 76 1387 1422 Size: 76 1311 35 Fragments arranged by size: 1311 76 35 XmaI C'CCGG_G Cuts at: 0 849 1422 Size: 849 573 XmnI GAAnn'nnTTC Cuts at: 0 193 1422 Size: 193 1229 Enzymes that do cut: AccI AcuI AfeI AflIII AlwNI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI BtgI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MluI MmeI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PfoI PpiI PpuMI PspXI SacI SacII SexAI SfoI SmaI SmlI StyI TatI TstI Tth111I XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssSI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MscI MslI NdeI NheI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI ZraI