Restriction map for
pFN21AE2366
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpuAI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MfeI MluI MroI MspA1I
MunI MvnI NarI NciI NdeI
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 18 GTCGAC GT^[AC][GT]AC
AcsI 589 AAATTC [GA]^AATT[CT]
AlwNI 61 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 589 AAATTC [GA]^AATT[CT]
AspI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 455 GGACC G^G[AT]CC
BalI 561 TGGCCA TGG^CCA
BfaI 503 CTAG C^TAG
BlpI 66 GCTGAGC GC^T[ACGT]AGC
BmyI 700 GGGCAC G[AGT]GC[ACT]^C
BpmI 101 CTGGAG CTGGAG(16/14)
Bpu1102I 66 GCTGAGC GC^T[ACGT]AGC
BsaAI 412 TACGTG [CT]AC^GT[GA]
BsaJI 564 CCATGG C^C[ACGT][ACGT]GG
BsaWI 395 TCCGGT [AT]^CCGG[AT]
BsmBI 104 GAGACG CGTCTC(1/5)
Bsp1286I 700 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 1276 TGTACA T^GTACA
BsrDI 996 GCAATG GCAATG(2/0)
BsrFI 27 GCCGGC [GA]^CCGG[CT]
BsrGI 1276 TGTACA T^GTACA
BstAPI 1194 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 66 GCTGAGC GC^T[ACGT]AGC
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
Cfr13I 455 GGACC G^G[ACGT]CC
DsaI 564 CCATGG C^C[GA][CT]GG
EcoT14I 564 CCATGG C^C[AT][AT]GG
FauI 31 GCGGG CCCGC(4/6)
HaeII 1379 GGCGCT [GA]GCGC^[CT]
HgaI 118 GACGC GACGC(5/10)
HincII 18 GTCGAC GT[CT]^[GA]AC
HindII 18 GTCGAC GT[CT]^[GA]AC
HphI 742 GGTGA GGTGA(8/7)
MaeI 503 CTAG C^TAG
MaeII 413 ACGT A^CGT
MluNI 561 TGGCCA TGG^CCA
MscI 561 TGGCCA TGG^CCA
NaeI 27 GCCGGC GCC^GGC
NcoI 564 CCATGG C^CATGG
NgoMIV 27 GCCGGC G^CCGGC
NspI 41 ACATGC [GA]CATG^[CT]
PflFI 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1287 GACTC GAGTC(4/5)
SalI 18 GTCGAC G^TCGAC
Sau96I 455 GGACC G^G[ACGT]CC
SgrAI 26 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 262 CTCAAG C^T[CT][GA]AG
SspBI 1276 TGTACA T^GTACA
StyI 564 CCATGG C^C[AT][AT]GG
Tth111I 118 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 502 TCTAGA T^CTAGA
XcmI 279 CCAACCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 31 GCGG CCGC(-3/-1)
49 GCGG
BbvI 1340 GCAGC GCAGC(8/12)
1306 GCTGC
BsiYI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1370 CCAGTATTTGG
BslI 283 CCATTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1370 CCAGTATTTGG
BsmI 813 GAATGC GAATGC(1/-1)
1343 GCATTC
BssKI 187 CCTGG ^CC[ACGT]GG
1072 CCTGG
BstNI 187 CCTGG CC^[AT]GG
1072 CCTGG
CfoI 25 GCGC GCG^C
1380 GCGC
EcoRII 187 CCTGG ^CC[AT]GG
1072 CCTGG
HapII 28 CCGG C^CGG
396 CCGG
HhaI 25 GCGC GCG^C
1380 GCGC
HinP1I 25 GCGC G^CGC
1380 GCGC
HpaII 28 CCGG C^CGG
396 CCGG
MspI 28 CCGG C^CGG
396 CCGG
MvaI 187 CCTGG CC^[AT]GG
1072 CCTGG
PflMI 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1370 CCAGTATTTGG
ScrFI 187 CCTGG CC^[ACGT]GG
1072 CCTGG
SfcI 344 CTGTAG C^T[GA][CT]AG
491 CTGTAG
SspI 236 AATATT AAT^ATT
681 AATATT
TseI 1306 GCTGC G^C[AT]GC
1340 GCAGC
Van91I 283 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1370 CCAGTATTTGG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AfaI AluI AlwI BsaBI BsmAI
BsrI BstF5I BstYI Cac8I DdeI
DpnII EaeI Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MwoI NdeII NlaIII RsaI Sau3AI
SfaNI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XhoII