(Circular) (Six-base) MAPSORT of: pFN21AE2366.seq Check: 9624 from: 1 to: 6103 pFN21AE2366 6103 bp With 182 enzymes: SgfI * August 4, 2011 13:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3913 278 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2024 3446 1937 Size: 87 1422 4594 Fragments arranged by size: 4594 1422 87 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4166 5550 5818 1632 Size: 2534 1384 268 1917 Fragments arranged by size: 2534 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5906 AflIII A'CryG_T Cuts at: 1203 5002 5979 1203 Size: 3799 977 1327 Fragments arranged by size: 3799 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 2678 1166 Size: 1512 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2071 3846 5418 2071 Size: 1775 1572 2756 Fragments arranged by size: 2756 1775 1572 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 2594 3412 3638 3790 5899 1493 Size: 1101 818 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 818 226 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 3428 1870 Size: 73 1485 4545 Fragments arranged by size: 4545 1485 73 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3424 4879 618 Size: 325 217 627 1637 1455 1842 Fragments arranged by size: 1842 1637 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3422 1391 Size: 2031 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5132 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4294 4328 1903 Size: 34 2357 34 3678 Fragments arranged by size: 3678 2357 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6098 6098 Size: 6103 BlpI GC'TnA_GC Cuts at: 2072 3520 2072 Size: 1448 4655 Bme1580I G_kGCm'C Cuts at: 1790 2709 4164 5320 1790 Size: 919 1455 1156 2573 Fragments arranged by size: 2573 1455 1156 919 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 3834 4792 Size: 416 53 83 399 24 1691 726 958 Cuts at: 4792 442 Size: 1753 Fragments arranged by size: 1753 1691 958 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2502 2502 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2127 4763 1239 Size: 888 2636 2579 Fragments arranged by size: 2636 2579 888 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2252 4228 5093 5391 2252 Size: 1976 865 298 2964 Fragments arranged by size: 2964 1976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4766 882 Size: 617 3267 2219 Fragments arranged by size: 3267 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2419 3894 493 Size: 711 1215 1475 2702 Fragments arranged by size: 2702 1475 1215 711 BsaBI GATnn'nnATC Cuts at: 2613 2736 2844 6097 2613 Size: 123 108 3253 2619 Fragments arranged by size: 3253 2619 123 108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3910 4292 4927 275 Size: 53 83 186 936 2377 382 635 1451 Fragments arranged by size: 2377 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2400 4536 4934 5208 5355 5766 2400 Size: 2136 398 274 147 411 2737 Fragments arranged by size: 2737 2136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5878 Fragments arranged by size: 5878 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6103 BseYI C'CCAG_C Cuts at: 3387 5306 5802 3387 Size: 1919 496 3688 Fragments arranged by size: 3688 1919 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4164 4249 5320 4164 Size: 85 1071 4947 Fragments arranged by size: 4947 1071 85 BsmI GAATG_Cn' Cuts at: 2824 3348 3614 3707 2824 Size: 524 266 93 5220 Fragments arranged by size: 5220 524 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2103 2103 Size: 6103 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2709 3422 4164 4249 5320 1391 Size: 399 919 713 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 713 399 85 BspEI T'CCGG_A Cuts at: 5766 5766 Size: 6103 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3458 844 Size: 2614 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3990 1394 Size: 429 2167 3507 Fragments arranged by size: 3507 2167 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3008 4598 4772 65 Size: 1183 1760 1590 174 1396 Fragments arranged by size: 1760 1590 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 4747 4934 1700 Size: 110 222 2715 187 2869 Fragments arranged by size: 2869 2715 222 187 110 BsrGI T'GTAC_A Cuts at: 96 3281 96 Size: 3185 2918 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 3205 794 Size: 2411 3692 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6103 BstYI r'GATC_y Cuts at: 1972 2329 2504 2608 3013 3262 3433 3800 4185 Size: 357 175 104 405 249 171 367 385 Cuts at: 4185 4202 5654 5740 5752 6098 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 405 385 367 357 346 249 175 171 104 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2569 513 Size: 552 1504 4047 Fragments arranged by size: 4047 1504 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2956 487 Size: 683 56 1730 3634 Fragments arranged by size: 3634 1730 683 56 BtsI GCAGTG_nn' Cuts at: 2083 2552 3619 4413 4433 2083 Size: 469 1067 794 20 3753 Fragments arranged by size: 3753 1067 794 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2566 2974 Size: 54 1425 50 275 18 225 511 408 Cuts at: 2974 3550 4439 6068 8 Size: 576 889 1629 43 Fragments arranged by size: 1629 1425 889 576 511 408 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4033 1353 Size: 2680 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2127 4166 4763 5550 5818 1239 Size: 393 495 2039 597 787 268 1524 Fragments arranged by size: 2039 1524 787 597 495 393 268 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 3388 5250 1678 Size: 1710 1862 2531 Fragments arranged by size: 2531 1862 1710 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2515 2547 2659 2691 3005 Size: 32 135 32 943 32 112 32 314 Cuts at: 3005 3037 4744 4776 4818 4850 1373 Size: 32 1707 32 42 32 2626 Fragments arranged by size: 2626 1707 943 314 135 112 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2025 3447 3693 669 Size: 988 281 87 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 87 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5217 5401 1305 Size: 3912 184 2007 Fragments arranged by size: 3912 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2568 10 Size: 54 1425 343 736 3545 Fragments arranged by size: 3545 1425 736 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 2678 2712 3303 4061 4420 4579 Size: 648 1408 104 34 591 758 359 159 Cuts at: 4579 518 Size: 2042 Fragments arranged by size: 2042 1408 758 648 591 359 159 104 34 MspA1I CmG'CkG Cuts at: 1606 3519 4196 5344 5589 1606 Size: 1913 677 1148 245 2120 Fragments arranged by size: 2120 1913 1148 677 245 NaeI GCC'GGC Cuts at: 2034 2034 Size: 6103 NcoI C'CATG_G Cuts at: 513 1065 2569 513 Size: 552 1504 4047 Fragments arranged by size: 4047 1504 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NgoMIV G'CCGG_C Cuts at: 2032 2032 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2050 3461 5006 1132 Size: 97 821 1411 1545 2229 Fragments arranged by size: 2229 1545 1411 821 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 2294 3381 1108 Size: 1186 1087 3830 Fragments arranged by size: 3830 1186 1087 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2703 2735 4172 4204 5685 5717 2703 Size: 32 1437 32 1481 32 3089 Fragments arranged by size: 3089 1481 1437 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 2844 3673 2844 Size: 829 5274 PstI C_TGCA'G Cuts at: 830 3455 830 Size: 2625 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 SalI G'TCGA_C Cuts at: 1936 2023 3445 1936 Size: 87 1422 4594 Fragments arranged by size: 4594 1422 87 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SfcI C'TryA_G Cuts at: 826 1045 2349 2496 3451 4586 5267 5458 826 Size: 219 1304 147 955 1135 681 191 1471 Fragments arranged by size: 1471 1304 1135 955 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 2032 1700 Size: 332 5771 SmaI CCC'GGG Cuts at: 1872 3430 1872 Size: 1558 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2267 4207 5108 5370 820 Size: 197 926 324 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 324 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 3461 1132 Size: 2329 3774 SspI AAT'ATT Cuts at: 5 52 2243 2688 4027 5 Size: 47 2191 445 1339 2081 Fragments arranged by size: 2191 2081 1339 445 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6103 StyI C'CwwG_G Cuts at: 513 1065 2569 3542 513 Size: 552 1504 973 3074 Fragments arranged by size: 3074 1504 973 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 4130 4147 4300 4485 4930 325 Size: 1255 1155 1395 17 153 185 445 1498 Fragments arranged by size: 1498 1395 1255 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3281 4349 6061 Size: 274 80 33 51 1443 1304 1068 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 1712 1443 1304 1068 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6063 6095 6063 Size: 32 6071 Tth111I GACn'n_nGTC Cuts at: 1316 2126 1316 Size: 810 5293 XbaI T'CTAG_A Cuts at: 2507 3439 2507 Size: 932 5171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2291 2291 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6103 XmaI C'CCGG_G Cuts at: 1870 3428 1870 Size: 1558 4545 XmnI GAAnn'nnTTC Cuts at: 4232 4232 Size: 6103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3911 276 Size: 53 83 186 936 2377 2468 Fragments arranged by size: 2468 2377 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI