################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII ApoI AscI AseI AsnI Asp718I AspI BbrPI BcgI BclI BfrI BsaBI BseAI BsiEI BsmBI BspDI BspEI BspHI BstBI ClaI CpoI DraI DrdI EagI EclXI Eco52I EcoRI FbaI FseI HgaI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SfuI SnaBI SpeI SspI SwaI TfiI Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 1278 GTATAC GT^[AC][GT]AC AcyI 1791 GGCGCC G[GA]^CG[CT]C AflIII 3044 ACATGT A^C[GA][CT]GT AgeI 1809 ACCGGT A^CCGGT AhdI 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3229 GTGCAC G^TGCAC ApaLI 3229 GTGCAC G^TGCAC Asp700I 3337 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2651 TGCGCA TGC^GCA AvrII 361 CCTAGG C^CTAGG BalI 2378 TGGCCA TGG^CCA BbeI 1791 GGCGCC GGCGC^C BbsI 3 GAAGAC GAAGAC(2/6) BbvCI 2194 GCTGAGG CCTCAGC(-5/-2) BciVI 1969 GGATAC GTATCC(6/5) BglII 224 AGATCT A^GATCT BlnI 361 CCTAGG C^CTAGG BpuAI 3 GAAGAC GAAGAC(2/6) BsaAI 2716 TACGTG [CT]AC^GT[GA] BsaHI 1791 GGCGCC G[GA]^CG[CT]C BsiWI 1916 CGTACG C^GTACG BsmI 2551 GAATGC GAATGC(1/-1) Bsp1407I 2771 TGTACA T^GTACA BspLU11I 3044 ACATGT A^CATGT BsrGI 2771 TGTACA T^GTACA Bst1107I 1278 GTATAC GTA^TAC BstEII 1821 GGTCACC G^GT[ACGT]ACC BstPI 1821 GGTCACC G^GT[ACGT]ACC BstXI 830 CCACTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1278 GTATAC GTA^TAC DraIII 3505 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 22 GACTCGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1821 GGTCACC G^GT[ACGT]ACC EcoT22I 935 ATGCAT ATGCA^T FspI 2651 TGCGCA TGC^GCA Hin1I 1791 GGCGCC G[GA]^CG[CT]C HincII 199 GTCAAC GT[CT]^[GA]AC HindII 199 GTCAAC GT[CT]^[GA]AC KasI 1791 GGCGCC G^GCGCC MluNI 2378 TGGCCA TGG^CCA MscI 2378 TGGCCA TGG^CCA MseI 338 TTAA T^TAA MslI 831 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1791 GGCGCC GG^CGCC NheI 541 GCTAGC G^CTAGC NsiI 935 ATGCAT ATGCA^T PinAI 1809 ACCGGT A^CCGGT SbfI 2038 CCTGCAGG CCTGCA^GG SfoI 1791 GGCGCC GGC^GCC SgrAI 1808 CACCGGTG C[GA]^CCGG[CT]G SphI 3486 GCATGC GCATG^C Sse8387I 2038 CCTGCAGG CCTGCA^GG SspBI 2771 TGTACA T^GTACA StuI 2112 AGGCCT AGG^CCT Tru9I 338 TTAA T^TAA XmnI 3337 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwNI 3215 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 3478 CAGTGGCTG Aor51HI 3012 AGCGCT AGC^GCT 3455 AGCGCT BamHI 2907 GGATCC G^GATCC 3275 GGATCC BlpI 3055 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC Bpu1102I 3055 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC BsaWI 1809 ACCGGT [AT]^CCGG[AT] 2544 TCCGGT BsrBI 2461 GAGCGG CCGCTC(-3/-3) 3058 GAGCGG BssSI 3328 CACGAG CACGAG(-5/-1) 2328 CTCGTG CelII 3055 GCTGAGC GC^T[ACGT]AGC 3445 GCTGAGC Eco47III 3012 AGCGCT AGC^GCT 3455 AGCGCT EcoRV 367 GATATC GAT^ATC 578 GATATC NaeI 2190 GCCGGC GCC^GGC 3381 GCCGGC NcoI 590 CCATGG C^CATGG 1001 CCATGG NgoMIV 2190 GCCGGC G^CCGGC 3381 GCCGGC NspI 3044 ACATGT [GA]CATG^[CT] 3486 GCATGC SexAI 975 ACCTGGT A^CC[AT]GGT 2396 ACCTGGT SfiI 1687 GGCCCCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2947 GGCCCACCTGGCC XcmI 618 CCAGGCTCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 966 CCAACCACTACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AocI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI