(Circular) (Six-base) MAPSORT of: pFN21AE4592.seq Check: 1577 from: 1 to: 8278 pFN21AE4592 8278 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2926 3660 4051 4111 4150 5196 5202 2926 Size: 734 391 60 39 1046 6 6002 Fragments arranged by size: 6002 1046 734 391 60 39 6 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6088 278 Size: 53 83 186 936 4552 2468 Fragments arranged by size: 4552 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 5621 1937 Size: 1347 2337 4594 Fragments arranged by size: 4594 2337 1347 Acc65I G'GTAC_C Cuts at: 5599 5599 Size: 8278 AclI AA'CG_TT Cuts at: 1563 6405 6778 1563 Size: 4842 373 3063 Fragments arranged by size: 4842 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5573 6341 7725 7993 1632 Size: 3941 768 1384 268 1917 Fragments arranged by size: 3941 1917 1384 768 268 AfeI AGC'GCT Cuts at: 5019 5462 5019 Size: 443 7835 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8081 AflIII A'CryG_T Cuts at: 1203 5049 7177 8154 1203 Size: 3846 2128 977 1327 Fragments arranged by size: 3846 2128 1327 977 AgeI A'CCGG_T Cuts at: 3814 7109 3814 Size: 3295 4983 AhdI GACnn_n'nnGTC Cuts at: 2032 7007 2032 Size: 4975 3303 AleI CACnn'nnGTG Cuts at: 1166 2840 1166 Size: 1674 6604 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8278 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4815 4847 4815 Size: 32 8246 AlwNI CAG_nnn'CTG Cuts at: 5225 5488 6021 7593 5225 Size: 263 533 1572 5910 Fragments arranged by size: 5910 1572 533 263 ApaI G_GGCC'C Cuts at: 2073 2468 2641 2744 2971 3053 3409 3534 3788 Size: 395 173 103 227 82 356 125 254 Cuts at: 3788 3915 4095 4113 4574 4998 2073 Size: 127 180 18 461 424 5353 Fragments arranged by size: 5353 461 424 395 356 254 227 180 173 127 125 103 82 18 ApaLI G'TGCA_C Cuts at: 5234 6335 7491 5234 Size: 1101 1156 6021 Fragments arranged by size: 6021 1156 1101 ApoI r'AATT_y Cuts at: 1493 5587 5813 5965 8074 1493 Size: 4094 226 152 2109 1697 Fragments arranged by size: 4094 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6832 160 Size: 872 5800 1606 Fragments arranged by size: 5800 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8278 AvaI C'yCGr_G Cuts at: 1870 1943 2073 2175 3943 3983 4217 4744 4797 Size: 73 130 102 1768 40 234 527 53 Cuts at: 4797 5603 1870 Size: 806 4545 Fragments arranged by size: 4545 1768 806 527 234 130 102 73 53 40 AvrII C'CTAG_G Cuts at: 2366 2366 Size: 8278 BamHI G'GATC_C Cuts at: 4912 5280 5608 5975 4912 Size: 368 328 367 7215 Fragments arranged by size: 7215 368 367 328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3796 3817 4228 5599 7054 Size: 325 217 627 2009 21 411 1371 1455 Cuts at: 7054 618 Size: 1842 Fragments arranged by size: 2009 1842 1455 1371 627 411 325 217 21 BanII G_rGCy'C Cuts at: 1391 2073 2468 2604 2641 2744 2971 3053 3409 Size: 682 395 136 37 103 227 82 356 Cuts at: 3409 3534 3788 3915 3937 4073 4095 4113 4226 Size: 125 254 127 22 136 22 18 113 Cuts at: 4226 4376 4574 4752 4998 5597 1391 Size: 150 198 178 246 599 4072 Fragments arranged by size: 4072 682 599 395 356 254 246 227 198 178 150 136 136 127 125 113 103 82 37 22 22 18 BbeI G_GCGC'C Cuts at: 3800 3800 Size: 8278 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 928 Size: 971 116 7191 Fragments arranged by size: 7191 971 116 BbvCI CC'TCA_GC Cuts at: 4200 4200 Size: 8278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6469 6503 1903 Size: 34 4532 34 3678 Fragments arranged by size: 4532 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3968 6170 7380 3968 Size: 2202 1210 4866 Fragments arranged by size: 4866 2202 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8278 BfrBI ATG'CAT Cuts at: 2942 2942 Size: 8278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 3690 3699 Size: 107 122 71 631 605 499 1519 9 Cuts at: 3699 3869 4959 6889 136 Size: 170 1090 1930 1525 Fragments arranged by size: 1930 1525 1519 1090 631 605 499 170 122 107 71 9 BglII A'GATC_T Cuts at: 2229 8273 2229 Size: 6044 2234 BlpI GC'TnA_GC Cuts at: 5061 5451 5695 5061 Size: 390 244 7644 Fragments arranged by size: 7644 390 244 Bme1580I G_kGCm'C Cuts at: 1790 2073 2468 2641 2675 2744 2971 3053 3409 Size: 283 395 173 34 69 227 82 356 Cuts at: 3409 3534 3788 3915 4095 4113 4233 4574 4581 Size: 125 254 127 180 18 120 341 7 Cuts at: 4581 4626 4728 4998 5238 6339 7495 1790 Size: 45 102 270 240 1101 1156 2573 Fragments arranged by size: 2573 1156 1101 395 356 341 283 270 254 240 227 180 173 127 125 120 102 82 69 45 34 18 7 BmgBI CAC'GTC Cuts at: 1318 2124 2397 1318 Size: 806 273 7199 Fragments arranged by size: 7199 806 273 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2397 3403 3411 Size: 416 53 83 399 24 980 1006 8 Cuts at: 3411 4568 6009 6967 442 Size: 1157 1441 958 1753 Fragments arranged by size: 1753 1441 1157 1006 980 958 416 399 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 2550 1056 Size: 1494 6784 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2248 2452 2206 Size: 42 204 8032 Fragments arranged by size: 8032 204 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2823 3087 3564 3584 3690 4388 4632 Size: 891 693 264 477 20 106 698 244 Cuts at: 4632 5021 6938 1239 Size: 389 1917 2579 Fragments arranged by size: 2579 1917 891 698 693 477 389 264 244 106 20 Bpu10I CC'TnA_GC Cuts at: 4169 4200 4533 4169 Size: 31 333 7914 Fragments arranged by size: 7914 333 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3729 3774 4829 6403 7268 7566 3729 Size: 45 1055 1574 865 298 4441 Fragments arranged by size: 4441 1574 1055 865 298 45 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2246 2920 3624 4205 4821 5271 6941 Size: 617 747 674 704 581 616 450 1670 Cuts at: 6941 882 Size: 2219 Fragments arranged by size: 2219 1670 747 704 674 617 616 581 450 BsaAI yAC'GTr Cuts at: 493 1204 4723 6069 493 Size: 711 3519 1346 2702 Fragments arranged by size: 3519 2702 1346 711 BsaBI GATnn'nnATC Cuts at: 8272 8272 Size: 8278 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3797 6085 6467 7102 Size: 53 83 186 936 2264 2288 382 635 Cuts at: 7102 275 Size: 1451 Fragments arranged by size: 2288 2264 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3814 4549 6711 7109 7383 7530 7941 3814 Size: 735 2162 398 274 147 411 4151 Fragments arranged by size: 4151 2162 735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3046 3076 3310 3340 4815 Size: 30 165 30 1643 30 234 30 1475 Cuts at: 4815 4845 5153 5183 1178 Size: 30 308 30 4273 Fragments arranged by size: 4273 1643 1475 308 234 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2240 2264 2619 3151 3169 3330 3528 Size: 870 184 24 355 532 18 161 198 Cuts at: 3528 4217 1186 Size: 689 5247 Fragments arranged by size: 5247 870 689 532 355 198 184 161 24 18 BseYI C'CCAG_C Cuts at: 2467 3202 3221 4234 4277 5027 5290 7481 7977 Size: 735 19 1013 43 750 263 2191 496 Cuts at: 7977 2467 Size: 2768 Fragments arranged by size: 2768 2191 1013 750 735 496 263 43 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4949 5174 5180 5315 5492 1163 Size: 573 3213 225 6 135 177 3949 Fragments arranged by size: 3949 3213 573 225 177 135 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5728 6489 7517 1540 Size: 275 186 3727 761 1028 2301 Fragments arranged by size: 3727 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2815 4549 5238 6339 6424 7495 2815 Size: 1734 689 1101 85 1071 3598 Fragments arranged by size: 3598 1734 1101 1071 689 85 BsiWI C'GTAC_G Cuts at: 3921 3921 Size: 8278 BsmI GAATG_Cn' Cuts at: 4562 5789 5882 4562 Size: 1227 93 6958 Fragments arranged by size: 6958 1227 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2468 2604 2641 2675 2744 2815 Size: 399 283 395 136 37 34 69 71 Cuts at: 2815 2971 3053 3409 3534 3788 3915 3937 4073 Size: 156 82 356 125 254 127 22 136 Cuts at: 4073 4095 4113 4226 4233 4376 4549 4574 4581 Size: 22 18 113 7 143 173 25 7 Cuts at: 4581 4626 4728 4752 4998 5238 5597 6339 6424 Size: 45 102 24 246 240 359 742 85 Cuts at: 6424 7495 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 395 359 356 283 254 246 240 173 156 143 136 136 127 125 113 102 85 82 71 69 45 37 34 25 24 22 22 18 7 7 BspEI T'CCGG_A Cuts at: 7941 7941 Size: 8278 BspHI T'CATG_A Cuts at: 6167 7897 6167 Size: 1730 6548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2913 2926 3660 4051 4111 4150 5196 5202 Size: 2069 13 734 391 60 39 1046 6 Cuts at: 5202 5391 5633 844 Size: 189 242 3489 Fragments arranged by size: 3489 2069 1046 734 391 242 189 60 39 13 6 BsrBI CCG'CTC Cuts at: 1394 1823 4468 5065 6165 1394 Size: 429 2645 597 1100 3507 Fragments arranged by size: 3507 2645 1100 597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3215 3376 3858 5119 6773 6947 65 Size: 1183 1967 161 482 1261 1654 174 1396 Fragments arranged by size: 1967 1654 1396 1261 1183 482 174 161 BsrFI r'CCGG_y Cuts at: 1700 1810 3814 4195 5386 6922 7109 1700 Size: 110 2004 381 1191 1536 187 2869 Fragments arranged by size: 2869 2004 1536 1191 381 187 110 BsrGI T'GTAC_A Cuts at: 96 4776 96 Size: 4680 3598 BssHII G'CGCG_C Cuts at: 1925 4338 4340 4439 1925 Size: 2413 2 99 5764 Fragments arranged by size: 5764 2413 99 2 BssSI C'ACGA_G Cuts at: 4333 5333 6338 7350 4333 Size: 1000 1005 1012 5261 Fragments arranged by size: 5261 1012 1005 1000 BstAPI GCAn_nnn'nTGC Cuts at: 794 5225 5434 5488 5515 794 Size: 4431 209 54 27 3557 Fragments arranged by size: 4431 3557 209 54 27 BstBI TT'CG_AA Cuts at: 7082 7082 Size: 8278 BstEII G'GTnAC_C Cuts at: 1184 3826 1184 Size: 2642 5636 BstXI CCAn_nnnn'nTGG Cuts at: 2842 2842 Size: 8278 BstYI r'GATC_y Cuts at: 1972 2229 4912 5280 5608 5975 6360 6377 7829 Size: 257 2683 368 328 367 385 17 1452 Cuts at: 7829 7915 7927 8273 1972 Size: 86 12 346 1977 Fragments arranged by size: 2683 1977 1452 385 368 367 346 328 257 86 17 12 BstZ17I GTA'TAC Cuts at: 3285 3285 Size: 8278 Bsu36I CC'TnA_GG Cuts at: 4114 4156 5260 4114 Size: 42 1104 7132 Fragments arranged by size: 7132 1104 42 BtgI C'CryG_G Cuts at: 513 1065 2595 3006 3852 3867 4080 513 Size: 552 1530 411 846 15 213 4711 Fragments arranged by size: 4711 1530 846 552 411 213 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4110 4568 487 Size: 683 56 2884 458 4197 Fragments arranged by size: 4197 2884 683 458 56 BtsI GCAGTG_nn' Cuts at: 2137 4612 4743 4789 5489 5794 6588 6608 2137 Size: 2475 131 46 700 305 794 20 3807 Fragments arranged by size: 3807 2475 794 700 305 131 46 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3841 3876 529 Size: 35 3277 35 4931 Fragments arranged by size: 4931 3277 35 35 DraI TTT'AAA Cuts at: 5582 5582 Size: 8278 DraIII CAC_nnn'GTG Cuts at: 5515 5515 Size: 8278 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7285 809 Size: 507 5969 1802 Fragments arranged by size: 5969 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2297 3349 4383 Size: 54 1425 50 275 18 467 1052 1034 Cuts at: 4383 5725 6614 8243 8 Size: 1342 889 1629 43 Fragments arranged by size: 1629 1425 1342 1052 1034 889 467 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5725 1537 Size: 275 3913 4090 Fragments arranged by size: 4090 3913 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3100 3169 3187 3718 3724 3733 3739 3841 Size: 1747 69 18 531 6 9 6 102 Cuts at: 3841 6208 1353 Size: 2367 3423 Fragments arranged by size: 3423 2367 1747 531 102 69 18 9 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2350 6865 7239 7385 387 Size: 1064 138 761 4515 374 146 1280 Fragments arranged by size: 4515 1280 1064 761 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2823 3087 3564 3584 3690 4388 Size: 393 498 693 264 477 20 106 698 Cuts at: 4388 4632 5021 5573 6341 6938 7725 7993 1239 Size: 244 389 552 768 597 787 268 1524 Fragments arranged by size: 1524 787 768 698 693 597 552 498 477 393 389 268 264 244 106 20 EcoNI CCTnn'n_nnAGG Cuts at: 2459 4155 5555 2459 Size: 1696 1400 5182 Fragments arranged by size: 5182 1696 1400 EcoO109I rG'GnC_Cy Cuts at: 2062 2070 2464 2637 2638 2705 2740 2741 2967 Size: 8 394 173 1 67 35 1 226 Cuts at: 2967 3049 3326 3383 3405 3531 3627 3692 3750 Size: 82 277 57 22 126 96 65 58 Cuts at: 3750 3784 3785 3912 3948 4011 4056 4085 4092 Size: 34 1 127 36 63 45 29 7 Cuts at: 4092 4109 4110 4637 4894 4933 4994 4995 5380 Size: 17 1 527 257 39 61 1 385 Cuts at: 5380 2062 Size: 4960 Fragments arranged by size: 4960 527 394 385 277 257 226 173 127 126 96 82 67 65 63 61 58 57 45 39 36 35 34 29 22 17 8 7 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 5587 5965 5587 Size: 378 7900 EcoRV GAT'ATC Cuts at: 2374 2585 2374 Size: 211 8067 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4870 4870 Size: 8278 FspI TGC'GCA Cuts at: 4658 6784 4658 Size: 2126 6152 HaeII r_GCGC'y Cuts at: 1678 3110 3800 4176 5021 5464 5471 7425 1678 Size: 1432 690 376 845 443 7 1954 2531 Fragments arranged by size: 2531 1954 1432 845 690 443 376 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2050 2426 2458 3310 Size: 32 135 32 446 32 376 32 852 Cuts at: 3310 3342 5140 5172 6919 6951 6993 7025 1373 Size: 32 1798 32 1747 32 42 32 2626 Fragments arranged by size: 2626 1798 1747 852 446 376 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 5622 5868 669 Size: 988 281 268 3416 246 3079 Fragments arranged by size: 3416 3079 988 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8278 HpaI GTT'AAC Cuts at: 5868 5868 Size: 8278 KasI G'GCGC_C Cuts at: 3796 3796 Size: 8278 KpnI G_GTAC'C Cuts at: 5603 5603 Size: 8278 MfeI C'AATT_G Cuts at: 5877 5877 Size: 8278 MluI A'CGCG_T Cuts at: 8154 8154 Size: 8278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2198 3747 7392 7576 1305 Size: 893 1549 3645 184 2007 Fragments arranged by size: 3645 2007 1549 893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4385 10 Size: 54 1425 343 2553 3903 Fragments arranged by size: 3903 2553 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2840 6236 6595 6754 518 Size: 648 1674 3396 359 159 2042 Fragments arranged by size: 3396 2042 1674 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4793 5027 5075 5294 5425 5450 5506 5521 Size: 3187 234 48 219 131 25 56 15 Cuts at: 5521 5694 6371 7519 7764 1606 Size: 173 677 1148 245 2120 Fragments arranged by size: 3187 2120 1148 677 245 234 219 173 131 56 48 25 15 NaeI GCC'GGC Cuts at: 4197 5388 4197 Size: 1191 7087 NarI GG'CG_CC Cuts at: 3797 3797 Size: 8278 NcoI C'CATG_G Cuts at: 513 1065 2595 3006 513 Size: 552 1530 411 5785 Fragments arranged by size: 5785 1530 552 411 NdeI CA'TA_TG Cuts at: 387 387 Size: 8278 NgoMIV G'CCGG_C Cuts at: 4195 5386 4195 Size: 1191 7087 NheI G'CTAG_C Cuts at: 1052 2546 1052 Size: 1494 6784 NotI GC'GGCC_GC Cuts at: 5725 5725 Size: 8278 NsiI A_TGCA'T Cuts at: 2944 2944 Size: 8278 NspI r_CATG'y Cuts at: 1132 1229 5053 5495 5636 7181 1132 Size: 97 3824 442 141 1545 2229 Fragments arranged by size: 3824 2229 1545 442 141 97 PasI CC'CwG_GG Cuts at: 1348 2066 2461 3613 3781 4052 4729 4898 5107 Size: 718 395 1152 168 271 677 169 209 Cuts at: 5107 1348 Size: 4519 Fragments arranged by size: 4519 1152 718 677 395 271 209 169 168 PciI A'CATG_T Cuts at: 5049 7177 5049 Size: 2128 6150 PflMI CCAn_nnn'nTGG Cuts at: 1108 2846 2981 3007 3562 1108 Size: 1738 135 26 555 5824 Fragments arranged by size: 5824 1738 555 135 26 PfoI T'CCnGG_A Cuts at: 3541 3541 Size: 8278 PmeI GTTT'AAAC Cuts at: 5582 5582 Size: 8278 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4815 4847 6347 6379 7860 7892 4815 Size: 32 1500 32 1481 32 5201 Fragments arranged by size: 5201 1500 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2705 3326 3750 4011 4056 4085 4637 4894 5380 Size: 621 424 261 45 29 552 257 486 Cuts at: 5380 2705 Size: 5603 Fragments arranged by size: 5603 621 552 486 424 261 257 45 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8278 PsiI TTA'TAA Cuts at: 5848 5848 Size: 8278 PspOMI G'GGCC_C Cuts at: 2069 2464 2637 2740 2967 3049 3405 3530 3784 Size: 395 173 103 227 82 356 125 254 Cuts at: 3784 3911 4091 4109 4570 4994 2069 Size: 127 180 18 461 424 5353 Fragments arranged by size: 5353 461 424 395 356 254 227 180 173 127 125 103 82 18 PstI C_TGCA'G Cuts at: 830 3154 4048 4947 5136 5430 5460 5630 830 Size: 2324 894 899 189 294 30 170 3478 Fragments arranged by size: 3478 2324 899 894 294 189 170 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8278 PvuII CAG'CTG Cuts at: 4793 5027 5075 5294 5425 5450 4793 Size: 234 48 219 131 25 7621 Fragments arranged by size: 7621 234 219 131 48 25 SalI G'TCGA_C Cuts at: 1936 5620 1936 Size: 3684 4594 SanDI GG'GwC_CC Cuts at: 2705 3326 4011 4894 2705 Size: 621 685 883 6089 Fragments arranged by size: 6089 883 685 621 SbfI CC_TGCA'GG Cuts at: 4048 5630 4048 Size: 1582 6696 ScaI AGT'ACT Cuts at: 6526 8238 6526 Size: 1712 6566 SexAI A'CCwGG_T Cuts at: 2980 4401 2980 Size: 1421 6857 SfcI C'TryA_G Cuts at: 826 1045 2410 3150 3679 4044 4617 4943 5132 Size: 219 1365 740 529 365 573 326 189 Cuts at: 5132 5426 5456 5542 5626 6761 7442 7633 826 Size: 294 30 86 84 1135 681 191 1471 Fragments arranged by size: 1471 1365 1135 740 681 573 529 365 326 294 219 191 189 86 84 30 SfiI GGCCn_nnn'nGGCC Cuts at: 3699 4959 3699 Size: 1260 7018 SfoI GGC'GCC Cuts at: 3798 3798 Size: 8278 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8278 SgrAI Cr'CCGG_yG Cuts at: 1700 3814 1700 Size: 2114 6164 SmaI CCC'GGG Cuts at: 1872 2075 3945 3985 4746 4799 5605 1872 Size: 203 1870 40 761 53 806 4545 Fragments arranged by size: 4545 1870 806 761 203 53 40 SmlI C'TyrA_G Cuts at: 820 1017 1943 3744 3789 4844 6382 7283 7545 Size: 197 926 1801 45 1055 1538 901 262 Cuts at: 7545 820 Size: 1553 Fragments arranged by size: 1801 1553 1538 1055 926 901 262 197 45 SnaBI TAC'GTA Cuts at: 493 6069 493 Size: 5576 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8278 SphI G_CATG'C Cuts at: 1132 5495 5636 1132 Size: 4363 141 3774 Fragments arranged by size: 4363 3774 141 SspI AAT'ATT Cuts at: 5 52 6202 5 Size: 47 6150 2081 Fragments arranged by size: 6150 2081 47 StuI AGG'CCT Cuts at: 1517 4119 1517 Size: 2602 5676 StyI C'CwwG_G Cuts at: 513 1065 2366 2595 2709 3006 4260 5717 513 Size: 552 1301 229 114 297 1254 1457 3074 Fragments arranged by size: 3074 1457 1301 1254 552 297 229 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2499 2590 2667 2785 2834 3011 3438 Size: 1255 919 91 77 118 49 177 427 Cuts at: 3438 3573 6305 6322 6475 6660 7105 325 Size: 135 2732 17 153 185 445 1498 Fragments arranged by size: 2732 1498 1255 919 445 427 185 177 153 135 118 91 77 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4353 4776 6524 Size: 274 80 33 51 1443 2376 423 1748 Cuts at: 6524 8236 96 Size: 1712 138 Fragments arranged by size: 2376 1748 1712 1443 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5153 5185 8238 8270 5153 Size: 32 3053 32 5161 Fragments arranged by size: 5161 3053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8278 XbaI T'CTAG_A Cuts at: 5614 5614 Size: 8278 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2630 2978 2630 Size: 348 7930 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8278 XmaI C'CCGG_G Cuts at: 1870 2073 3943 3983 4744 4797 5603 1870 Size: 203 1870 40 761 53 806 4545 Fragments arranged by size: 4545 1870 806 761 203 53 40 XmnI GAAnn'nnTTC Cuts at: 5346 6407 5346 Size: 1061 7217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6086 276 Size: 53 83 186 936 4552 2468 Fragments arranged by size: 4552 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsmBI EcoICRI FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SrfI SwaI