(Linear) (Six-base) MAPSORT of: KIEE4592.seq Check: 176 from: 1 to: 3573 KIEE4592.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 921 1655 2046 2106 2145 3191 3197 3573 Size: 921 734 391 60 39 1046 6 376 Fragments arranged by size: 1046 921 734 391 376 60 39 6 AccI GT'mk_AC Cuts at: 0 1279 3573 Size: 1279 2294 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3568 3573 Size: 3568 5 AfeI AGC'GCT Cuts at: 0 3014 3457 3573 Size: 3014 443 116 Fragments arranged by size: 3014 443 116 AflIII A'CryG_T Cuts at: 0 3044 3573 Size: 3044 529 AgeI A'CCGG_T Cuts at: 0 1809 3573 Size: 1809 1764 AhdI GACnn_n'nnGTC Cuts at: 0 27 3573 Size: 27 3546 AleI CACnn'nnGTG Cuts at: 0 835 3573 Size: 835 2738 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2810 2842 3573 Size: 2810 32 731 Fragments arranged by size: 2810 731 32 AlwNI CAG_nnn'CTG Cuts at: 0 3220 3483 3573 Size: 3220 263 90 Fragments arranged by size: 3220 263 90 ApaI G_GGCC'C Cuts at: 0 68 463 636 739 966 1048 1404 1529 Size: 68 395 173 103 227 82 356 125 Cuts at: 1529 1783 1910 2090 2108 2569 2993 3573 Size: 254 127 180 18 461 424 580 Fragments arranged by size: 580 461 424 395 356 254 227 180 173 127 125 103 82 68 18 ApaLI G'TGCA_C Cuts at: 0 3229 3573 Size: 3229 344 AvaI C'yCGr_G Cuts at: 0 68 170 1938 1978 2212 2739 2792 3573 Size: 68 102 1768 40 234 527 53 781 Fragments arranged by size: 1768 781 527 234 102 68 53 40 AvrII C'CTAG_G Cuts at: 0 361 3573 Size: 361 3212 BamHI G'GATC_C Cuts at: 0 2907 3275 3573 Size: 2907 368 298 Fragments arranged by size: 2907 368 298 BanI G'GyrC_C Cuts at: 0 1791 1812 2223 3573 Size: 1791 21 411 1350 Fragments arranged by size: 1791 1350 411 21 BanII G_rGCy'C Cuts at: 0 68 463 599 636 739 966 1048 1404 Size: 68 395 136 37 103 227 82 356 Cuts at: 1404 1529 1783 1910 1932 2068 2090 2108 2221 Size: 125 254 127 22 136 22 18 113 Cuts at: 2221 2371 2569 2747 2993 3573 Size: 150 198 178 246 580 Fragments arranged by size: 580 395 356 254 246 227 198 178 150 136 136 127 125 113 103 82 68 37 22 22 18 BbeI G_GCGC'C Cuts at: 0 1795 3573 Size: 1795 1778 BbsI GAAGACnn'nnnn_ Cuts at: 0 10 3573 Size: 10 3563 BbvCI CC'TCA_GC Cuts at: 0 2195 3573 Size: 2195 1378 BciVI GTATCCnnnnn_n' Cuts at: 0 1963 3573 Size: 1963 1610 BfrBI ATG'CAT Cuts at: 0 937 3573 Size: 937 2636 BglI GCCn_nnn'nGGC Cuts at: 0 166 1685 1694 1864 2954 3573 Size: 166 1519 9 170 1090 619 Fragments arranged by size: 1519 1090 619 170 166 9 BglII A'GATC_T Cuts at: 0 224 3573 Size: 224 3349 BlpI GC'TnA_GC Cuts at: 0 3056 3446 3573 Size: 3056 390 127 Fragments arranged by size: 3056 390 127 Bme1580I G_kGCm'C Cuts at: 0 68 463 636 670 739 966 1048 1404 Size: 68 395 173 34 69 227 82 356 Cuts at: 1404 1529 1783 1910 2090 2108 2228 2569 2576 Size: 125 254 127 180 18 120 341 7 Cuts at: 2576 2621 2723 2993 3233 3573 Size: 45 102 270 240 340 Fragments arranged by size: 395 356 341 340 270 254 240 227 180 173 127 125 120 102 82 69 68 45 34 18 7 BmgBI CAC'GTC Cuts at: 0 119 392 3573 Size: 119 273 3181 Fragments arranged by size: 3181 273 119 BmrI ACTGGGnnnn_n' Cuts at: 0 392 1398 1406 2563 3573 Size: 392 1006 8 1157 1010 Fragments arranged by size: 1157 1010 1006 392 8 BmtI G_CTAG'C Cuts at: 0 545 3573 Size: 545 3028 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 201 243 447 3573 Size: 201 42 204 3126 Fragments arranged by size: 3126 204 201 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 818 1082 1559 1579 1685 2383 2627 Size: 125 693 264 477 20 106 698 244 Cuts at: 2627 3016 3573 Size: 389 557 Fragments arranged by size: 698 693 557 477 389 264 244 125 106 20 Bpu10I CC'TnA_GC Cuts at: 0 2164 2195 2528 3573 Size: 2164 31 333 1045 Fragments arranged by size: 2164 1045 333 31 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1724 1769 2824 3573 Size: 1724 45 1055 749 Fragments arranged by size: 1724 1055 749 45 BsaI GGTCTCn'nnnn_ Cuts at: 0 241 915 1619 2200 2816 3266 3573 Size: 241 674 704 581 616 450 307 Fragments arranged by size: 704 674 616 581 450 307 241 BsaAI yAC'GTr Cuts at: 0 2718 3573 Size: 2718 855 BsaHI Gr'CG_yC Cuts at: 0 1792 3573 Size: 1792 1781 BsaWI w'CCGG_w Cuts at: 0 1809 2544 3573 Size: 1809 735 1029 Fragments arranged by size: 1809 1029 735 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1041 1071 1305 1335 2810 2840 3148 3178 Size: 1041 30 234 30 1475 30 308 30 Cuts at: 3178 3573 Size: 395 Fragments arranged by size: 1475 1041 395 308 234 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 51 235 259 614 1146 1164 1325 1523 Size: 51 184 24 355 532 18 161 198 Cuts at: 1523 2212 3573 Size: 689 1361 Fragments arranged by size: 1361 689 532 355 198 184 161 51 24 18 BseYI C'CCAG_C Cuts at: 0 462 1197 1216 2229 2272 3022 3285 3573 Size: 462 735 19 1013 43 750 263 288 Fragments arranged by size: 1013 750 735 462 288 263 43 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2944 3169 3175 3310 3487 3573 Size: 2944 225 6 135 177 86 Fragments arranged by size: 2944 225 177 135 86 6 BsiHKAI G_wGCw'C Cuts at: 0 810 2544 3233 3573 Size: 810 1734 689 340 Fragments arranged by size: 1734 810 689 340 BsiWI C'GTAC_G Cuts at: 0 1916 3573 Size: 1916 1657 BsmI GAATG_Cn' Cuts at: 0 2557 3573 Size: 2557 1016 Bsp1286I G_dGCh'C Cuts at: 0 68 463 599 636 670 739 810 966 Size: 68 395 136 37 34 69 71 156 Cuts at: 966 1048 1404 1529 1783 1910 1932 2068 2090 Size: 82 356 125 254 127 22 136 22 Cuts at: 2090 2108 2221 2228 2371 2544 2569 2576 2621 Size: 18 113 7 143 173 25 7 45 Cuts at: 2621 2723 2747 2993 3233 3573 Size: 102 24 246 240 340 Fragments arranged by size: 395 356 340 254 246 240 173 156 143 136 136 127 125 113 102 82 71 69 68 45 37 34 25 24 22 22 18 7 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 908 921 1655 2046 2106 2145 3191 3197 Size: 908 13 734 391 60 39 1046 6 Cuts at: 3197 3386 3573 Size: 189 187 Fragments arranged by size: 1046 908 734 391 189 187 60 39 13 6 BsrBI CCG'CTC Cuts at: 0 2463 3060 3573 Size: 2463 597 513 Fragments arranged by size: 2463 597 513 BsrDI GCAATG_nn' Cuts at: 0 1210 1371 1853 3114 3573 Size: 1210 161 482 1261 459 Fragments arranged by size: 1261 1210 482 459 161 BsrFI r'CCGG_y Cuts at: 0 1809 2190 3381 3573 Size: 1809 381 1191 192 Fragments arranged by size: 1809 1191 381 192 BsrGI T'GTAC_A Cuts at: 0 2771 3573 Size: 2771 802 BssHII G'CGCG_C Cuts at: 0 2333 2335 2434 3573 Size: 2333 2 99 1139 Fragments arranged by size: 2333 1139 99 2 BssSI C'ACGA_G Cuts at: 0 2328 3328 3573 Size: 2328 1000 245 Fragments arranged by size: 2328 1000 245 BstAPI GCAn_nnn'nTGC Cuts at: 0 3220 3429 3483 3510 3573 Size: 3220 209 54 27 63 Fragments arranged by size: 3220 209 63 54 27 BstEII G'GTnAC_C Cuts at: 0 1821 3573 Size: 1821 1752 BstXI CCAn_nnnn'nTGG Cuts at: 0 837 3573 Size: 837 2736 BstYI r'GATC_y Cuts at: 0 224 2907 3275 3573 Size: 224 2683 368 298 Fragments arranged by size: 2683 368 298 224 BstZ17I GTA'TAC Cuts at: 0 1280 3573 Size: 1280 2293 Bsu36I CC'TnA_GG Cuts at: 0 2109 2151 3255 3573 Size: 2109 42 1104 318 Fragments arranged by size: 2109 1104 318 42 BtgI C'CryG_G Cuts at: 0 590 1001 1847 1862 2075 3573 Size: 590 411 846 15 213 1498 Fragments arranged by size: 1498 846 590 411 213 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2105 2563 3573 Size: 2105 458 1010 Fragments arranged by size: 2105 1010 458 BtsI GCAGTG_nn' Cuts at: 0 132 2607 2738 2784 3484 3573 Size: 132 2475 131 46 700 89 Fragments arranged by size: 2475 700 132 131 89 46 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1836 1871 3573 Size: 1836 35 1702 Fragments arranged by size: 1836 1702 35 DraIII CAC_nnn'GTG Cuts at: 0 3510 3573 Size: 3510 63 EaeI y'GGCC_r Cuts at: 0 292 1344 2378 3573 Size: 292 1052 1034 1195 Fragments arranged by size: 1195 1052 1034 292 EarI CTCTTCn'nnn_ Cuts at: 0 1095 1164 1182 1713 1719 1728 1734 1836 Size: 1095 69 18 531 6 9 6 102 Cuts at: 1836 3573 Size: 1737 Fragments arranged by size: 1737 1095 531 102 69 18 9 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 345 3573 Size: 345 3228 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 125 818 1082 1559 1579 1685 2383 2627 Size: 125 693 264 477 20 106 698 244 Cuts at: 2627 3016 3568 3573 Size: 389 552 5 Fragments arranged by size: 698 693 552 477 389 264 244 125 106 20 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 454 2150 3550 3573 Size: 454 1696 1400 23 Fragments arranged by size: 1696 1400 454 23 EcoO109I rG'GnC_Cy Cuts at: 0 57 65 459 632 633 700 735 736 Size: 57 8 394 173 1 67 35 1 Cuts at: 736 962 1044 1321 1378 1400 1526 1622 1687 Size: 226 82 277 57 22 126 96 65 Cuts at: 1687 1745 1779 1780 1907 1943 2006 2051 2080 Size: 58 34 1 127 36 63 45 29 Cuts at: 2080 2087 2104 2105 2632 2889 2928 2989 2990 Size: 7 17 1 527 257 39 61 1 Cuts at: 2990 3375 3573 Size: 385 198 Fragments arranged by size: 527 394 385 277 257 226 198 173 127 126 96 82 67 65 63 61 58 57 57 45 39 36 35 34 29 22 17 8 7 1 1 1 1 1 EcoRV GAT'ATC Cuts at: 0 369 580 3573 Size: 369 211 2993 Fragments arranged by size: 2993 369 211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2865 3573 Size: 2865 708 FspI TGC'GCA Cuts at: 0 2653 3573 Size: 2653 920 HaeII r_GCGC'y Cuts at: 0 1105 1795 2171 3016 3459 3466 3573 Size: 1105 690 376 845 443 7 107 Fragments arranged by size: 1105 845 690 443 376 107 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 13 45 421 453 1305 1337 3135 3167 Size: 13 32 376 32 852 32 1798 32 Cuts at: 3167 3573 Size: 406 Fragments arranged by size: 1798 852 406 376 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 201 3573 Size: 201 3372 KasI G'GCGC_C Cuts at: 0 1791 3573 Size: 1791 1782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1742 3573 Size: 193 1549 1831 Fragments arranged by size: 1831 1549 193 MscI TGG'CCA Cuts at: 0 2380 3573 Size: 2380 1193 MslI CAynn'nnrTG Cuts at: 0 835 3573 Size: 835 2738 MspA1I CmG'CkG Cuts at: 0 2788 3022 3070 3289 3420 3445 3501 3516 Size: 2788 234 48 219 131 25 56 15 Cuts at: 3516 3573 Size: 57 Fragments arranged by size: 2788 234 219 131 57 56 48 25 15 NaeI GCC'GGC Cuts at: 0 2192 3383 3573 Size: 2192 1191 190 Fragments arranged by size: 2192 1191 190 NarI GG'CG_CC Cuts at: 0 1792 3573 Size: 1792 1781 NcoI C'CATG_G Cuts at: 0 590 1001 3573 Size: 590 411 2572 Fragments arranged by size: 2572 590 411 NgoMIV G'CCGG_C Cuts at: 0 2190 3381 3573 Size: 2190 1191 192 Fragments arranged by size: 2190 1191 192 NheI G'CTAG_C Cuts at: 0 541 3573 Size: 541 3032 NsiI A_TGCA'T Cuts at: 0 939 3573 Size: 939 2634 NspI r_CATG'y Cuts at: 0 3048 3490 3573 Size: 3048 442 83 Fragments arranged by size: 3048 442 83 PasI CC'CwG_GG Cuts at: 0 61 456 1608 1776 2047 2724 2893 3102 Size: 61 395 1152 168 271 677 169 209 Cuts at: 3102 3573 Size: 471 Fragments arranged by size: 1152 677 471 395 271 209 169 168 61 PciI A'CATG_T Cuts at: 0 3044 3573 Size: 3044 529 PflMI CCAn_nnn'nTGG Cuts at: 0 841 976 1002 1557 3573 Size: 841 135 26 555 2016 Fragments arranged by size: 2016 841 555 135 26 PfoI T'CCnGG_A Cuts at: 0 1536 3573 Size: 1536 2037 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2810 2842 3573 Size: 2810 32 731 Fragments arranged by size: 2810 731 32 PpuMI rG'GwC_Cy Cuts at: 0 700 1321 1745 2006 2051 2080 2632 2889 Size: 700 621 424 261 45 29 552 257 Cuts at: 2889 3375 3573 Size: 486 198 Fragments arranged by size: 700 621 552 486 424 261 257 198 45 29 PspOMI G'GGCC_C Cuts at: 0 64 459 632 735 962 1044 1400 1525 Size: 64 395 173 103 227 82 356 125 Cuts at: 1525 1779 1906 2086 2104 2565 2989 3573 Size: 254 127 180 18 461 424 584 Fragments arranged by size: 584 461 424 395 356 254 227 180 173 127 125 103 82 64 18 PstI C_TGCA'G Cuts at: 0 1149 2043 2942 3131 3425 3455 3573 Size: 1149 894 899 189 294 30 118 Fragments arranged by size: 1149 899 894 294 189 118 30 PvuII CAG'CTG Cuts at: 0 2788 3022 3070 3289 3420 3445 3573 Size: 2788 234 48 219 131 25 128 Fragments arranged by size: 2788 234 219 131 128 48 25 SanDI GG'GwC_CC Cuts at: 0 700 1321 2006 2889 3573 Size: 700 621 685 883 684 Fragments arranged by size: 883 700 685 684 621 SbfI CC_TGCA'GG Cuts at: 0 2043 3573 Size: 2043 1530 SexAI A'CCwGG_T Cuts at: 0 975 2396 3573 Size: 975 1421 1177 Fragments arranged by size: 1421 1177 975 SfcI C'TryA_G Cuts at: 0 405 1145 1674 2039 2612 2938 3127 3421 Size: 405 740 529 365 573 326 189 294 Cuts at: 3421 3451 3537 3573 Size: 30 86 36 Fragments arranged by size: 740 573 529 405 365 326 294 189 86 36 30 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1694 2954 3573 Size: 1694 1260 619 Fragments arranged by size: 1694 1260 619 SfoI GGC'GCC Cuts at: 0 1793 3573 Size: 1793 1780 SgrAI Cr'CCGG_yG Cuts at: 0 1809 3573 Size: 1809 1764 SmaI CCC'GGG Cuts at: 0 70 1940 1980 2741 2794 3573 Size: 70 1870 40 761 53 779 Fragments arranged by size: 1870 779 761 70 53 40 SmlI C'TyrA_G Cuts at: 0 1739 1784 2839 3573 Size: 1739 45 1055 734 Fragments arranged by size: 1739 1055 734 45 SphI G_CATG'C Cuts at: 0 3490 3573 Size: 3490 83 StuI AGG'CCT Cuts at: 0 2114 3573 Size: 2114 1459 StyI C'CwwG_G Cuts at: 0 361 590 704 1001 2255 3573 Size: 361 229 114 297 1254 1318 Fragments arranged by size: 1318 1254 361 297 229 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 494 585 662 780 829 1006 1433 1568 Size: 494 91 77 118 49 177 427 135 Cuts at: 1568 3573 Size: 2005 Fragments arranged by size: 2005 494 427 177 135 118 91 77 49 TatI w'GTAC_w Cuts at: 0 2348 2771 3573 Size: 2348 423 802 Fragments arranged by size: 2348 802 423 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3148 3180 3573 Size: 3148 32 393 Fragments arranged by size: 3148 393 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 625 973 3573 Size: 625 348 2600 Fragments arranged by size: 2600 625 348 XmaI C'CCGG_G Cuts at: 0 68 1938 1978 2739 2792 3573 Size: 68 1870 40 761 53 781 Fragments arranged by size: 1870 781 761 68 53 40 XmnI GAAnn'nnTTC Cuts at: 0 3341 3573 Size: 3341 232 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SanDI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AlfI ApoI AscI AseI AsiSI BaeI BcgI BclI BsaBI BsiEI BsmBI BspEI BspHI BstBI ClaI DraI DrdI EagI EcoICRI EcoRI FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NotI NruI PacI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI