Restriction map for
pFN21AE5622
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BanII BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FauI FseI FspI HgaI HincII
HindII HindIII HpaI HphI KpnI
KspI MluI MroI MslI MvnI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 319 GTCTAC GT^[AC][GT]AC
AciI 1113 CCGC CCGC(-3/-1)
AcyI 597 GGCGCC G[GA]^CG[CT]C
AflIII 960 ACATGT A^C[GA][CT]GT
AlwNI 245 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 325 CCTCAGG CC^T[ACGT]AGG
Asp700I 1360 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 546 GAGCAC G[AT]GC[AT]^C
AvaII 855 GGACC G^G[AT]CC
BamHI 829 GGATCC G^GATCC
BanI 597 GGCGCC G^G[CT][GA]CC
BbeI 597 GGCGCC GGCGC^C
BcnI 720 CCGGG CC^[GC]GG
BmyI 546 GAGCAC G[AGT]GC[ACT]^C
BsaBI 916 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 597 GGCGCC G[GA]^CG[CT]C
BsaWI 1118 ACCGGA [AT]^CCGG[AT]
BsgI 602 CTGCAC GTGCAG(16/14)
BsiHKAI 546 GAGCAC G[AT]GC[AT]^C
Bsp1286I 546 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 960 ACATGT A^CATGT
BspMI 606 ACCTGC ACCTGC(4/8)
BstYI 829 GGATCC [GA]^GATC[CT]
Bsu36I 325 CCTCAGG CC^T[ACGT]AGG
CfoI 598 GCGC GCG^C
Cfr13I 855 GGACC G^G[ACGT]CC
DraII 854 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1056 CCATGG C^C[GA][CT]GG
Eco81I 325 CCTCAGG CC^T[ACGT]AGG
EcoNI 1329 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 854 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 384 GATATC GAT^ATC
HaeII 597 GGCGCC [GA]GCGC^[CT]
HhaI 598 GCGC GCG^C
Hin1I 597 GGCGCC G[GA]^CG[CT]C
HinP1I 598 GCGC G^CGC
KasI 597 GGCGCC G^GCGCC
MamI 916 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 401 CAATTG C^AATTG
MflI 829 GGATCC [GA]^GATC[CT]
MspA1I 1315 CAGCTG C[AC]G^C[GT]G
MunI 401 CAATTG C^AATTG
NarI 597 GGCGCC GG^CGCC
NciI 720 CCGGG CC^[GC]GG
NcoI 1056 CCATGG C^CATGG
NdeI 1454 CATATG CA^TATG
PpuMI 854 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1315 CAGCTG CAG^CTG
SapI 7 GAAGAGC GCTCTTC(1/4)
Sau96I 855 GGACC G^G[ACGT]CC
SexAI 953 ACCAGGT A^CC[AT]GGT
SfoI 597 GGCGCC GGC^GCC
SmlI 999 CTTGAG C^T[CT][GA]AG
StuI 488 AGGCCT AGG^CCT
XhoII 829 GGATCC [GA]^GATC[CT]
XmnI 1360 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 483 GTAC GT^AC
958 GTAC
AlwI 830 GATCC GGATC(4/5)
829 GGATC
BalI 990 TGGCCA TGG^CCA
1331 TGGCCA
BpmI 504 CTCCAG CTGGAG(16/14)
629 CTGGAG
BsaI 632 GAGACC GGTCTC(1/5)
1165 GGTCTC
BseRI 1168 CTCCTC GAGGAG(10/8)
943 GAGGAG
BsiYI 1179 CCAGAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1329 CCTGGCCAAGG
BslI 1179 CCAGAGGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1329 CCTGGCCAAGG
EaeI 990 TGGCCA [CT]^GGCC[GA]
1331 TGGCCA
EarI 1564 CTCTTC CTCTTC(1/4)
7 GAAGAG
EcoT14I 1056 CCATGG C^C[AT][AT]GG
1334 CCAAGG
HapII 720 CCGG C^CGG
1119 CCGG
HpaII 720 CCGG C^CGG
1119 CCGG
Ksp632I 1564 CTCTTC CTCTTC(1/4)
7 GAAGAG
MaeII 340 ACGT A^CGT
1157 ACGT
MluNI 990 TGGCCA TGG^CCA
1331 TGGCCA
MscI 990 TGGCCA TGG^CCA
1331 TGGCCA
MspI 720 CCGG C^CGG
1119 CCGG
NspI 163 ACATGC [GA]CATG^[CT]
960 ACATGT
PstI 608 CTGCAG CTGCA^G
1195 CTGCAG
RsaI 483 GTAC GT^AC
958 GTAC
SspI 409 AATATT AAT^ATT
1362 AATATT
StyI 1056 CCATGG C^C[AT][AT]GG
1334 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AluI BbvI BclI BfaI BsaJI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstXI Cac8I DdeI DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI