(Circular) (Six-base) MAPSORT of: pFN21AE5622.seq Check: 3115 from: 1 to: 6313 pFN21AE5622 6313 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2620 2620 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 3656 1937 Size: 388 1331 4594 Fragments arranged by size: 4594 1331 388 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4376 5760 6028 1632 Size: 2744 1384 268 1917 Fragments arranged by size: 2744 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 2965 5212 6189 1203 Size: 1762 2247 977 1327 Fragments arranged by size: 2247 1762 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3556 1184 Size: 2372 3941 AlwNI CAG_nnn'CTG Cuts at: 2255 4056 5628 2255 Size: 1801 1572 2940 Fragments arranged by size: 2940 1801 1572 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 3622 3848 4000 6109 1493 Size: 2129 226 152 2109 1697 Fragments arranged by size: 2129 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 3638 1870 Size: 73 1695 4545 Fragments arranged by size: 4545 1695 73 BamHI G'GATC_C Cuts at: 2834 3643 4010 2834 Size: 809 367 5137 Fragments arranged by size: 5137 809 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2602 3634 5089 618 Size: 325 217 627 815 1032 1455 1842 Fragments arranged by size: 1842 1455 1032 815 627 325 217 BanII G_rGCy'C Cuts at: 1391 3632 1391 Size: 2241 4072 BbeI G_GCGC'C Cuts at: 2606 2606 Size: 6313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 2107 2692 3306 1548 Size: 559 585 614 4555 Fragments arranged by size: 4555 614 585 559 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4924 136 Size: 107 122 71 631 605 3252 1525 Fragments arranged by size: 3252 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6308 6308 Size: 6313 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 4374 5530 1790 Size: 2584 1156 2573 Fragments arranged by size: 2584 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4044 5002 442 Size: 416 53 83 399 24 2627 958 1753 Fragments arranged by size: 2627 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2494 2655 4973 1239 Size: 1255 161 2318 2579 Fragments arranged by size: 2579 2318 1255 161 Bpu10I CC'TnA_GC Cuts at: 2881 3043 3593 2881 Size: 162 550 5601 Fragments arranged by size: 5601 550 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3025 4438 5303 5601 3025 Size: 1413 865 298 3737 Fragments arranged by size: 3737 1413 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2631 3176 4976 882 Size: 617 1132 545 1800 2219 Fragments arranged by size: 2219 1800 1132 617 545 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 2925 6307 2925 Size: 3382 2931 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2603 4120 4502 5137 Size: 53 83 186 936 1070 1517 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 1517 1451 1070 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3123 4746 5144 5418 5565 5976 3123 Size: 1623 398 274 147 411 3460 Fragments arranged by size: 3460 1623 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4969 Fragments arranged by size: 4969 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2963 3164 1186 Size: 1777 201 4335 Fragments arranged by size: 4335 1777 201 BseYI C'CCAG_C Cuts at: 2504 3318 5516 6012 2504 Size: 814 2198 496 2805 Fragments arranged by size: 2805 2198 814 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2592 1163 Size: 573 856 4884 Fragments arranged by size: 4884 856 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3763 4524 5552 1540 Size: 275 186 1762 761 1028 2301 Fragments arranged by size: 2301 1762 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2555 4374 4459 5530 2555 Size: 1819 85 1071 3338 Fragments arranged by size: 3338 1819 1071 85 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2555 3632 4374 4459 5530 1391 Size: 399 765 1077 742 85 1071 2174 Fragments arranged by size: 2174 1077 1071 765 742 399 85 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 4202 5932 4202 Size: 1730 4583 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2620 3668 844 Size: 1776 1048 3489 Fragments arranged by size: 3489 1776 1048 BsrBI CCG'CTC Cuts at: 1394 1823 4200 1394 Size: 429 2377 3507 Fragments arranged by size: 3507 2377 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4808 4982 65 Size: 1183 3560 174 1396 Fragments arranged by size: 3560 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4957 5144 1700 Size: 110 3147 187 2869 Fragments arranged by size: 3147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6313 BstBI TT'CG_AA Cuts at: 5117 5117 Size: 6313 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 2348 2852 3322 3584 2348 Size: 504 470 262 5077 Fragments arranged by size: 5077 504 470 262 BstYI r'GATC_y Cuts at: 1972 2834 3643 4010 4395 4412 5864 5950 5962 Size: 862 809 367 385 17 1452 86 12 Cuts at: 5962 6308 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 862 809 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2331 2331 Size: 6313 BtgI C'CryG_G Cuts at: 513 1065 2004 3061 513 Size: 552 939 1057 3765 Fragments arranged by size: 3765 1057 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5574 Fragments arranged by size: 5574 683 56 BtsI GCAGTG_nn' Cuts at: 3197 3829 4623 4643 3197 Size: 632 794 20 4867 Fragments arranged by size: 4867 794 632 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3148 3183 529 Size: 35 2584 35 3659 Fragments arranged by size: 3659 2584 35 35 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5320 809 Size: 507 4004 1802 Fragments arranged by size: 4004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2995 3336 3760 Size: 54 1425 50 275 18 1165 341 424 Cuts at: 3760 4649 6278 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1165 889 424 341 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3760 1537 Size: 275 1948 4090 Fragments arranged by size: 4090 1948 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2007 3575 4243 1353 Size: 654 1568 668 3423 Fragments arranged by size: 3423 1568 668 654 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2494 2655 4376 4973 5760 6028 1239 Size: 393 862 161 1721 597 787 268 1524 Fragments arranged by size: 1721 1524 862 787 597 393 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 3338 3338 Size: 6313 EcoO109I rG'GnC_Cy Cuts at: 2860 2860 Size: 6313 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 EcoRV GAT'ATC Cuts at: 2391 2391 Size: 6313 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2151 2673 2151 Size: 522 5791 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2606 5460 1678 Size: 928 2854 2531 Fragments arranged by size: 2854 2531 928 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2681 2713 4954 Size: 32 135 32 620 32 457 32 2241 Cuts at: 4954 4986 5028 5060 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2241 620 457 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KasI G'GCGC_C Cuts at: 2602 2602 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 2406 3912 2406 Size: 1506 4807 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 5427 5611 1305 Size: 1257 2865 184 2007 Fragments arranged by size: 2865 2007 1257 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2997 3338 10 Size: 54 1425 343 1165 341 2985 Fragments arranged by size: 2985 1425 1165 343 341 54 MslI CAynn'nnrTG Cuts at: 518 1166 4271 4630 4789 518 Size: 648 3105 359 159 2042 Fragments arranged by size: 3105 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3322 3729 4406 5554 5799 1606 Size: 1716 407 677 1148 245 2120 Fragments arranged by size: 2120 1716 1148 677 407 245 NarI GG'CG_CC Cuts at: 2603 2603 Size: 6313 NcoI C'CATG_G Cuts at: 513 1065 2004 3061 513 Size: 552 939 1057 3765 Fragments arranged by size: 3765 1057 939 552 NdeI CA'TA_TG Cuts at: 387 3460 387 Size: 3073 3240 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 2172 2969 3671 5216 1132 Size: 97 943 797 702 1545 2229 Fragments arranged by size: 2229 1545 943 797 702 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6313 PciI A'CATG_T Cuts at: 2965 5212 2965 Size: 2247 4066 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6313 PfoI T'CCnGG_A Cuts at: 2699 2699 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4382 4414 5895 5927 4382 Size: 32 1481 32 4768 Fragments arranged by size: 4768 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2860 2860 Size: 6313 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6313 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PstI C_TGCA'G Cuts at: 830 2617 3204 3665 830 Size: 1787 587 461 3478 Fragments arranged by size: 3478 1787 587 461 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 3322 3322 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SapI GCTCTTCn'nnn_ Cuts at: 2007 2007 Size: 6313 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SexAI A'CCwGG_T Cuts at: 2958 2958 Size: 6313 SfcI C'TryA_G Cuts at: 826 1045 2300 2613 3013 3200 3388 3661 4796 Size: 219 1255 313 400 187 188 273 1135 Cuts at: 4796 5477 5668 826 Size: 681 191 1471 Fragments arranged by size: 1471 1255 1135 681 400 313 273 219 191 188 187 SfoI GGC'GCC Cuts at: 2604 2604 Size: 6313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3004 4417 5318 5580 820 Size: 197 926 1061 1413 901 262 1553 Fragments arranged by size: 1553 1413 1061 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 3610 152 Size: 3458 2855 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 2416 3369 4237 5 Size: 47 2364 953 868 2081 Fragments arranged by size: 2364 2081 953 868 47 StuI AGG'CCT Cuts at: 1517 2495 1517 Size: 978 5335 StyI C'CwwG_G Cuts at: 513 1065 2004 3061 3339 3752 513 Size: 552 939 1057 278 413 3074 Fragments arranged by size: 3074 1057 939 552 413 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3065 3155 3332 4340 4357 4510 4695 Size: 1255 1485 90 177 1008 17 153 185 Cuts at: 4695 5140 325 Size: 445 1498 Fragments arranged by size: 1498 1485 1255 1008 445 185 177 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4559 6271 96 Size: 274 80 33 51 1443 2582 1712 138 Fragments arranged by size: 2582 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6273 6305 6273 Size: 32 6281 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6313 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 3369 4442 3369 Size: 1073 5240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI