(Linear) (Six-base) MAPSORT of: KIEE5622.seq Check: 8182 from: 1 to: 1608 KIEE5622.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 615 1608 Size: 615 993 AccI GT'mk_AC Cuts at: 0 320 1608 Size: 320 1288 AflIII A'CryG_T Cuts at: 0 960 1608 Size: 960 648 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1551 1608 Size: 1551 57 AlwNI CAG_nnn'CTG Cuts at: 0 250 1608 Size: 250 1358 BamHI G'GATC_C Cuts at: 0 829 1608 Size: 829 779 BanI G'GyrC_C Cuts at: 0 597 1608 Size: 597 1011 BbeI G_GCGC'C Cuts at: 0 601 1608 Size: 601 1007 BclI T'GATC_A Cuts at: 0 102 687 1301 1608 Size: 102 585 614 307 Fragments arranged by size: 614 585 307 102 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 650 1608 Size: 489 161 958 Fragments arranged by size: 958 489 161 Bpu10I CC'TnA_GC Cuts at: 0 876 1038 1588 1608 Size: 876 162 550 20 Fragments arranged by size: 876 550 162 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1020 1608 Size: 1020 588 BsaI GGTCTCn'nnnn_ Cuts at: 0 626 1171 1608 Size: 626 545 437 Fragments arranged by size: 626 545 437 BsaBI GATnn'nnATC Cuts at: 0 920 1608 Size: 920 688 BsaHI Gr'CG_yC Cuts at: 0 598 1608 Size: 598 1010 BsaWI w'CCGG_w Cuts at: 0 1118 1608 Size: 1118 490 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 487 517 1608 Size: 487 30 1091 Fragments arranged by size: 1091 487 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 958 1159 1608 Size: 958 201 449 Fragments arranged by size: 958 449 201 BseYI C'CCAG_C Cuts at: 0 499 1313 1608 Size: 499 814 295 Fragments arranged by size: 814 499 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 587 1608 Size: 587 1021 BsiHKAI G_wGCw'C Cuts at: 0 550 1608 Size: 550 1058 Bsp1286I G_dGCh'C Cuts at: 0 550 1608 Size: 550 1058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 615 1608 Size: 615 993 BstXI CCAn_nnnn'nTGG Cuts at: 0 343 847 1317 1579 1608 Size: 343 504 470 262 29 Fragments arranged by size: 504 470 343 262 29 BstYI r'GATC_y Cuts at: 0 829 1608 Size: 829 779 Bsu36I CC'TnA_GG Cuts at: 0 326 1608 Size: 326 1282 BtgI C'CryG_G Cuts at: 0 1056 1608 Size: 1056 552 BtsI GCAGTG_nn' Cuts at: 0 1192 1608 Size: 1192 416 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1143 1178 1608 Size: 1143 35 430 Fragments arranged by size: 1143 430 35 EaeI y'GGCC_r Cuts at: 0 990 1331 1608 Size: 990 341 277 Fragments arranged by size: 990 341 277 EarI CTCTTCn'nnn_ Cuts at: 0 2 1570 1608 Size: 2 1568 38 Fragments arranged by size: 1568 38 2 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 650 1608 Size: 489 161 958 Fragments arranged by size: 958 489 161 EcoNI CCTnn'n_nnAGG Cuts at: 0 1333 1608 Size: 1333 275 EcoO109I rG'GnC_Cy Cuts at: 0 855 1608 Size: 855 753 EcoRV GAT'ATC Cuts at: 0 386 1608 Size: 386 1222 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 146 668 1608 Size: 146 522 940 Fragments arranged by size: 940 522 146 HaeII r_GCGC'y Cuts at: 0 601 1608 Size: 601 1007 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 187 219 676 708 1608 Size: 187 32 457 32 900 Fragments arranged by size: 900 457 187 32 32 KasI G'GCGC_C Cuts at: 0 597 1608 Size: 597 1011 MfeI C'AATT_G Cuts at: 0 401 1608 Size: 401 1207 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 557 1608 Size: 557 1051 MscI TGG'CCA Cuts at: 0 992 1333 1608 Size: 992 341 275 Fragments arranged by size: 992 341 275 MspA1I CmG'CkG Cuts at: 0 1317 1608 Size: 1317 291 NarI GG'CG_CC Cuts at: 0 598 1608 Size: 598 1010 NcoI C'CATG_G Cuts at: 0 1056 1608 Size: 1056 552 NdeI CA'TA_TG Cuts at: 0 1455 1608 Size: 1455 153 NspI r_CATG'y Cuts at: 0 167 964 1608 Size: 167 797 644 Fragments arranged by size: 797 644 167 PciI A'CATG_T Cuts at: 0 960 1608 Size: 960 648 PfoI T'CCnGG_A Cuts at: 0 694 1608 Size: 694 914 PpuMI rG'GwC_Cy Cuts at: 0 855 1608 Size: 855 753 PstI C_TGCA'G Cuts at: 0 612 1199 1608 Size: 612 587 409 Fragments arranged by size: 612 587 409 PvuII CAG'CTG Cuts at: 0 1317 1608 Size: 1317 291 SapI GCTCTTCn'nnn_ Cuts at: 0 2 1608 Size: 2 1606 SexAI A'CCwGG_T Cuts at: 0 953 1608 Size: 953 655 SfcI C'TryA_G Cuts at: 0 295 608 1008 1195 1383 1608 Size: 295 313 400 187 188 225 Fragments arranged by size: 400 313 295 225 188 187 SfoI GGC'GCC Cuts at: 0 599 1608 Size: 599 1009 SmlI C'TyrA_G Cuts at: 0 999 1608 Size: 999 609 SspI AAT'ATT Cuts at: 0 411 1364 1608 Size: 411 953 244 Fragments arranged by size: 953 411 244 StuI AGG'CCT Cuts at: 0 490 1608 Size: 490 1118 StyI C'CwwG_G Cuts at: 0 1056 1334 1608 Size: 1056 278 274 Fragments arranged by size: 1056 278 274 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1060 1150 1327 1608 Size: 1060 90 177 281 Fragments arranged by size: 1060 281 177 90 XmnI GAAnn'nnTTC Cuts at: 0 1364 1608 Size: 1364 244 Enzymes that do cut: AarI AccI AflIII AlfI AlwNI BamHI BanI BbeI BclI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MfeI MmeI MscI MspA1I NarI NcoI NdeI NspI PciI PfoI PpuMI PstI PvuII SapI SexAI SfcI SfoI SmlI SspI StuI StyI TaqII XmnI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanII BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BsaAI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRI FseI FspI FspAI HincII HindIII HpaI KpnI MluI MslI NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI