(Circular) (Six-base) MAPSORT of: pFN21AB6270.seq Check: 3407 from: 1 to: 7021 pFN21AB6270 7021 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4831 278 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4364 1937 Size: 2427 4594 Acc65I G'GTAC_C Cuts at: 4342 4342 Size: 7021 AclI AA'CG_TT Cuts at: 1563 5148 5521 1563 Size: 3585 373 3063 Fragments arranged by size: 3585 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4202 5084 6468 6736 1632 Size: 2570 882 1384 268 1917 Fragments arranged by size: 2570 1917 1384 882 268 AfeI AGC'GCT Cuts at: 2425 2425 Size: 7021 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6824 AflIII A'CryG_T Cuts at: 1203 5920 6897 1203 Size: 4717 977 1327 Fragments arranged by size: 4717 1327 977 AgeI A'CCGG_T Cuts at: 5852 5852 Size: 7021 AhdI GACnn_n'nnGTC Cuts at: 5750 5750 Size: 7021 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7021 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4025 1184 Size: 2841 4180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2927 2959 2927 Size: 32 6989 AlwNI CAG_nnn'CTG Cuts at: 2962 3260 4087 4764 6336 2962 Size: 298 827 677 1572 3647 Fragments arranged by size: 3647 1572 827 677 298 ApaI G_GGCC'C Cuts at: 2200 2225 2200 Size: 25 6996 ApaLI G'TGCA_C Cuts at: 3134 3926 5078 6234 3134 Size: 792 1152 1156 3921 Fragments arranged by size: 3921 1156 1152 792 ApoI r'AATT_y Cuts at: 1493 4330 4556 4708 6817 1493 Size: 2837 226 152 2109 1697 Fragments arranged by size: 2837 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5575 160 Size: 872 4543 1606 Fragments arranged by size: 4543 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 AvaI C'yCGr_G Cuts at: 1870 1943 2153 4346 1870 Size: 73 210 2193 4545 Fragments arranged by size: 4545 2193 210 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4137 4170 4137 Size: 33 6988 BamHI G'GATC_C Cuts at: 4351 4718 4351 Size: 367 6654 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 3210 4342 5797 618 Size: 325 217 627 240 1183 1132 1455 1842 Fragments arranged by size: 1842 1455 1183 1132 627 325 240 217 BanII G_rGCy'C Cuts at: 1391 2200 2225 2436 2480 2980 3573 4340 1391 Size: 809 25 211 44 500 593 767 4072 Fragments arranged by size: 4072 809 767 593 500 211 44 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2903 4060 4169 928 Size: 971 1004 1157 109 3780 Fragments arranged by size: 3780 1157 1004 971 109 BbvCI CC'TCA_GC Cuts at: 2370 2787 3796 2370 Size: 417 1009 5595 Fragments arranged by size: 5595 1009 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5212 5246 1903 Size: 34 3275 34 3678 Fragments arranged by size: 3678 3275 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4913 6123 4913 Size: 1210 5811 BclI T'GATC_A Cuts at: 1548 1548 Size: 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3732 3852 3956 Size: 107 122 71 631 605 2060 120 104 Cuts at: 3956 5632 136 Size: 1676 1525 Fragments arranged by size: 2060 1676 1525 631 605 122 120 107 104 71 BglII A'GATC_T Cuts at: 2341 7016 2341 Size: 4675 2346 BlpI GC'TnA_GC Cuts at: 4438 4438 Size: 7021 Bme1580I G_kGCm'C Cuts at: 1790 2200 2225 3138 3668 3930 5082 6238 1790 Size: 410 25 913 530 262 1152 1156 2573 Fragments arranged by size: 2573 1156 1152 913 530 410 262 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7021 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2732 3157 3194 Size: 416 53 83 399 24 1315 425 37 Cuts at: 3194 3306 4752 5710 442 Size: 112 1446 958 1753 Fragments arranged by size: 1753 1446 1315 958 425 416 399 112 83 53 37 24 BmtI G_CTAG'C Cuts at: 1056 2559 1056 Size: 1503 5518 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 3592 2425 Size: 1167 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2241 2649 2971 3077 3389 3481 4259 Size: 836 166 408 322 106 312 92 778 Cuts at: 4259 5681 1239 Size: 1422 2579 Fragments arranged by size: 2579 1422 836 778 408 322 312 166 106 92 Bpu10I CC'TnA_GC Cuts at: 2370 2787 2979 3796 2370 Size: 417 192 817 5595 Fragments arranged by size: 5595 817 417 192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5146 6011 6309 5146 Size: 865 298 5858 Fragments arranged by size: 5858 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5684 882 Size: 617 4185 2219 Fragments arranged by size: 4185 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4812 493 Size: 711 3608 2702 Fragments arranged by size: 3608 2702 711 BsaBI GATnn'nnATC Cuts at: 2268 3443 7015 2268 Size: 1175 3572 2274 Fragments arranged by size: 3572 2274 1175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3865 4828 5210 5845 Size: 53 83 186 936 2332 963 382 635 Cuts at: 5845 275 Size: 1451 Fragments arranged by size: 2332 1451 963 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2097 3657 5454 5852 6126 6273 6684 2097 Size: 1560 1797 398 274 147 411 2434 Fragments arranged by size: 2434 1797 1560 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2927 2957 1178 Size: 30 165 30 1524 30 5242 Fragments arranged by size: 5242 1524 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2398 2426 2501 2504 2588 2657 2951 2954 Size: 1212 28 75 3 84 69 294 3 Cuts at: 2954 3543 4250 1186 Size: 589 707 3957 Fragments arranged by size: 3957 1212 707 589 294 84 75 69 28 3 3 BseYI C'CCAG_C Cuts at: 3179 3391 3572 3987 4185 6224 6720 3179 Size: 212 181 415 198 2039 496 3480 Fragments arranged by size: 3480 2039 496 415 212 198 181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2798 1163 Size: 573 1062 5386 Fragments arranged by size: 5386 1062 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4471 5232 6260 1540 Size: 275 186 1855 615 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 615 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 2980 3138 3527 3930 4208 5082 5167 6238 Size: 544 158 389 403 278 874 85 1071 Cuts at: 6238 2436 Size: 3219 Fragments arranged by size: 3219 1071 874 544 403 389 278 158 85 BsiWI C'GTAC_G Cuts at: 2056 2056 Size: 7021 BsmI GAATG_Cn' Cuts at: 2621 4532 4625 2621 Size: 1911 93 5017 Fragments arranged by size: 5017 1911 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2200 2225 2436 2480 2980 3138 3527 Size: 399 410 25 211 44 500 158 389 Cuts at: 3527 3573 3668 3930 4208 4340 5082 5167 6238 Size: 46 95 262 278 132 742 85 1071 Cuts at: 6238 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 500 410 399 389 278 262 211 158 132 95 85 46 44 25 BspEI T'CCGG_A Cuts at: 2097 6684 2097 Size: 4587 2434 BspHI T'CATG_A Cuts at: 4910 6640 4910 Size: 1730 5291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2970 4376 844 Size: 2126 1406 3489 Fragments arranged by size: 3489 2126 1406 BsrBI CCG'CTC Cuts at: 1394 1823 2386 3972 4908 1394 Size: 429 563 1586 936 3507 Fragments arranged by size: 3507 1586 936 563 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3721 5516 5690 65 Size: 1183 2473 1795 174 1396 Fragments arranged by size: 2473 1795 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3515 3762 5665 5852 1700 Size: 110 1705 247 1903 187 2869 Fragments arranged by size: 2869 1903 1705 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7021 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7021 BssSI C'ACGA_G Cuts at: 5081 6093 5081 Size: 1012 6009 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7021 BstBI TT'CG_AA Cuts at: 5825 5825 Size: 7021 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7021 BstXI CCAn_nnnn'nTGG Cuts at: 3207 3455 4157 3207 Size: 248 702 6071 Fragments arranged by size: 6071 702 248 BstYI r'GATC_y Cuts at: 1972 2263 2341 3444 4351 4718 5103 5120 6572 Size: 291 78 1103 907 367 385 17 1452 Cuts at: 6572 6658 6670 7016 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1103 907 385 367 346 291 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2410 2410 Size: 7021 BtgI C'CryG_G Cuts at: 513 1065 2023 2331 2508 2781 513 Size: 552 958 308 177 273 4753 Fragments arranged by size: 4753 958 552 308 273 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6282 Fragments arranged by size: 6282 683 56 BtsI GCAGTG_nn' Cuts at: 2550 3997 4537 5331 5351 2550 Size: 1447 540 794 20 4220 Fragments arranged by size: 4220 1447 794 540 20 ClaI AT'CG_AT Cuts at: 1554 4059 1554 Size: 2505 4516 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6986 DraI TTT'AAA Cuts at: 4325 4325 Size: 7021 DraIII CAC_nnn'GTG Cuts at: 3202 3202 Size: 7021 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2849 6028 809 Size: 507 1533 3179 1802 Fragments arranged by size: 3179 1802 1533 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3096 3408 3691 Size: 54 1425 50 275 18 1266 312 283 Cuts at: 3691 3853 4468 5357 6986 8 Size: 162 615 889 1629 43 Fragments arranged by size: 1629 1425 1266 889 615 312 283 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 4468 1537 Size: 275 2041 615 4090 Fragments arranged by size: 4090 2041 615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2008 2447 2681 3944 4121 4951 1353 Size: 655 439 234 1263 177 830 3423 Fragments arranged by size: 3423 1263 830 655 439 234 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 5608 5982 6128 387 Size: 1064 138 660 3359 374 146 1280 Fragments arranged by size: 3359 1280 1064 660 374 146 138 EcoICRI GAG'CTC Cuts at: 2434 2978 2434 Size: 544 6477 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2241 2649 2971 3077 3389 3481 Size: 393 443 166 408 322 106 312 92 Cuts at: 3481 4202 4259 5084 5681 6468 6736 1239 Size: 721 57 825 597 787 268 1524 Fragments arranged by size: 1524 825 787 721 597 443 408 393 322 312 268 166 106 92 57 EcoNI CCTnn'n_nnAGG Cuts at: 2598 2678 2922 2598 Size: 80 244 6697 Fragments arranged by size: 6697 244 80 EcoO109I rG'GnC_Cy Cuts at: 2051 2196 2197 2222 2275 2533 3300 3633 4195 Size: 145 1 25 53 258 767 333 562 Cuts at: 4195 2051 Size: 4877 Fragments arranged by size: 4877 767 562 333 258 145 53 25 1 EcoRI G'AATT_C Cuts at: 4330 4708 4330 Size: 378 6643 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 3466 2731 Size: 735 6286 FspI TGC'GCA Cuts at: 2966 5527 2966 Size: 2561 4460 HaeII r_GCGC'y Cuts at: 1678 2427 6168 1678 Size: 749 3741 2531 Fragments arranged by size: 3741 2531 749 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2054 2086 2477 2509 3004 Size: 32 135 32 482 32 391 32 495 Cuts at: 3004 3036 3523 3555 5662 5694 5736 5768 1373 Size: 32 487 32 2107 32 42 32 2626 Fragments arranged by size: 2626 2107 495 487 482 391 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3067 4365 4611 669 Size: 988 281 1129 1298 246 3079 Fragments arranged by size: 3079 1298 1129 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7021 HpaI GTT'AAC Cuts at: 4611 4611 Size: 7021 KpnI G_GTAC'C Cuts at: 4346 4346 Size: 7021 MfeI C'AATT_G Cuts at: 4620 4620 Size: 7021 MluI A'CGCG_T Cuts at: 6897 6897 Size: 7021 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2475 2715 6135 6319 1305 Size: 1170 240 3420 184 2007 Fragments arranged by size: 3420 2007 1170 240 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3098 3410 3693 10 Size: 54 1425 343 1266 312 283 3338 Fragments arranged by size: 3338 1425 1266 343 312 283 54 MslI CAynn'nnrTG Cuts at: 518 1166 2820 3453 3471 4979 5338 5497 518 Size: 648 1654 633 18 1508 359 159 2042 Fragments arranged by size: 2042 1654 1508 648 633 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2119 2333 2374 2949 4437 5114 6262 6507 Size: 513 214 41 575 1488 677 1148 245 Cuts at: 6507 1606 Size: 2120 Fragments arranged by size: 2120 1488 1148 677 575 513 245 214 41 NaeI GCC'GGC Cuts at: 3517 3764 3517 Size: 247 6774 NcoI C'CATG_G Cuts at: 513 1065 2023 2508 513 Size: 552 958 485 5026 Fragments arranged by size: 5026 958 552 485 NdeI CA'TA_TG Cuts at: 387 387 Size: 7021 NgoMIV G'CCGG_C Cuts at: 3515 3762 3515 Size: 247 6774 NheI G'CTAG_C Cuts at: 1052 2555 1052 Size: 1503 5518 NotI GC'GGCC_GC Cuts at: 4468 4468 Size: 7021 NspI r_CATG'y Cuts at: 1132 1229 4379 5924 1132 Size: 97 3150 1545 2229 Fragments arranged by size: 3150 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3031 3587 1348 Size: 1683 556 4782 Fragments arranged by size: 4782 1683 556 PciI A'CATG_T Cuts at: 5920 5920 Size: 7021 PflMI CCAn_nnn'nTGG Cuts at: 1108 2345 3202 1108 Size: 1237 857 4927 Fragments arranged by size: 4927 1237 857 PfoI T'CCnGG_A Cuts at: 2379 2679 4236 2379 Size: 300 1557 5164 Fragments arranged by size: 5164 1557 300 PmeI GTTT'AAAC Cuts at: 4325 4325 Size: 7021 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2927 2959 5090 5122 6603 6635 2927 Size: 32 2131 32 1481 32 3313 Fragments arranged by size: 3313 2131 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2051 2275 2533 3633 4195 2051 Size: 224 258 1100 562 4877 Fragments arranged by size: 4877 1100 562 258 224 PshAI GACnn'nnGTC Cuts at: 1613 4169 4247 1613 Size: 2556 78 4387 Fragments arranged by size: 4387 2556 78 PsiI TTA'TAA Cuts at: 4591 4591 Size: 7021 PspOMI G'GGCC_C Cuts at: 2196 2221 2196 Size: 25 6996 PstI C_TGCA'G Cuts at: 830 2916 3549 4373 830 Size: 2086 633 824 3478 Fragments arranged by size: 3478 2086 824 633 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7021 PvuII CAG'CTG Cuts at: 2374 2949 2374 Size: 575 6446 SacI G_AGCT'C Cuts at: 2436 2980 2436 Size: 544 6477 SacII CC_GC'GG Cuts at: 2334 2334 Size: 7021 SalI G'TCGA_C Cuts at: 1936 4363 1936 Size: 2427 4594 SbfI CC_TGCA'GG Cuts at: 4373 4373 Size: 7021 ScaI AGT'ACT Cuts at: 5269 6981 5269 Size: 1712 5309 SfcI C'TryA_G Cuts at: 826 1045 2912 3545 3610 4369 5504 6185 6376 Size: 219 1867 633 65 759 1135 681 191 Cuts at: 6376 826 Size: 1471 Fragments arranged by size: 1867 1471 1135 759 681 633 219 191 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7021 SmaI CCC'GGG Cuts at: 1872 4348 1872 Size: 2476 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5125 6026 6288 820 Size: 197 926 3182 901 262 1553 Fragments arranged by size: 3182 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4812 493 Size: 4319 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7021 SphI G_CATG'C Cuts at: 1132 4379 1132 Size: 3247 3774 SspI AAT'ATT Cuts at: 5 52 4945 5 Size: 47 4893 2081 Fragments arranged by size: 4893 2081 47 StuI AGG'CCT Cuts at: 1517 3794 1517 Size: 2277 4744 StyI C'CwwG_G Cuts at: 513 1065 2023 2508 3830 4460 513 Size: 552 958 485 1322 630 3074 Fragments arranged by size: 3074 1322 958 630 552 485 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 3122 3213 3513 5048 5065 5218 Size: 1255 612 930 91 300 1535 17 153 Cuts at: 5218 5403 5848 325 Size: 185 445 1498 Fragments arranged by size: 1535 1498 1255 930 612 445 300 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2766 5267 6979 Size: 274 80 33 51 1443 789 2501 1712 Cuts at: 6979 96 Size: 138 Fragments arranged by size: 2501 1712 1443 789 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4187 4219 6981 7013 4187 Size: 32 2762 32 4195 Fragments arranged by size: 4195 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7021 XbaI T'CTAG_A Cuts at: 4357 4357 Size: 7021 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7021 XmaI C'CCGG_G Cuts at: 1870 4346 1870 Size: 2476 4545 XmnI GAAnn'nnTTC Cuts at: 2714 5150 2714 Size: 2436 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4829 276 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BfrBI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI