(Linear) (Six-base) MAPSORT of: KIBB6270.seq Check: 6884 from: 1 to: 2316 KIBB6270.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2197 2316 Size: 2197 119 AfeI AGC'GCT Cuts at: 0 420 2316 Size: 420 1896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2020 2316 Size: 2020 296 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 922 954 2316 Size: 922 32 1362 Fragments arranged by size: 1362 922 32 AlwNI CAG_nnn'CTG Cuts at: 0 957 1255 2082 2316 Size: 957 298 827 234 Fragments arranged by size: 957 827 298 234 ApaI G_GGCC'C Cuts at: 0 195 220 2316 Size: 195 25 2096 Fragments arranged by size: 2096 195 25 ApaLI G'TGCA_C Cuts at: 0 1129 1921 2316 Size: 1129 792 395 Fragments arranged by size: 1129 792 395 AvaI C'yCGr_G Cuts at: 0 148 2316 Size: 148 2168 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2132 2165 2316 Size: 2132 33 151 Fragments arranged by size: 2132 151 33 BanI G'GyrC_C Cuts at: 0 22 1205 2316 Size: 22 1183 1111 Fragments arranged by size: 1183 1111 22 BanII G_rGCy'C Cuts at: 0 195 220 431 475 975 1568 2316 Size: 195 25 211 44 500 593 748 Fragments arranged by size: 748 593 500 211 195 44 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 898 2055 2164 2316 Size: 898 1157 109 152 Fragments arranged by size: 1157 898 152 109 BbvCI CC'TCA_GC Cuts at: 0 365 782 1791 2316 Size: 365 417 1009 525 Fragments arranged by size: 1009 525 417 365 BglI GCCn_nnn'nGGC Cuts at: 0 1727 1847 1951 2316 Size: 1727 120 104 365 Fragments arranged by size: 1727 365 120 104 BglII A'GATC_T Cuts at: 0 336 2316 Size: 336 1980 Bme1580I G_kGCm'C Cuts at: 0 195 220 1133 1663 1925 2316 Size: 195 25 913 530 262 391 Fragments arranged by size: 913 530 391 262 195 25 BmrI ACTGGGnnnn_n' Cuts at: 0 727 1152 1189 1301 2316 Size: 727 425 37 112 1015 Fragments arranged by size: 1015 727 425 112 37 BmtI G_CTAG'C Cuts at: 0 554 2316 Size: 554 1762 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 420 1587 2316 Size: 420 1167 729 Fragments arranged by size: 1167 729 420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 236 644 966 1072 1384 1476 2254 Size: 70 166 408 322 106 312 92 778 Cuts at: 2254 2316 Size: 62 Fragments arranged by size: 778 408 322 312 166 106 92 70 62 Bpu10I CC'TnA_GC Cuts at: 0 365 782 974 1791 2316 Size: 365 417 192 817 525 Fragments arranged by size: 817 525 417 365 192 BsaBI GATnn'nnATC Cuts at: 0 263 1438 2316 Size: 263 1175 878 Fragments arranged by size: 1175 878 263 BsaHI Gr'CG_yC Cuts at: 0 1860 2316 Size: 1860 456 BsaWI w'CCGG_w Cuts at: 0 92 1652 2316 Size: 92 1560 664 Fragments arranged by size: 1560 664 92 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 922 952 2316 Size: 922 30 1364 Fragments arranged by size: 1364 922 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 393 421 496 499 583 652 946 949 Size: 393 28 75 3 84 69 294 3 Cuts at: 949 1538 2245 2316 Size: 589 707 71 Fragments arranged by size: 707 589 393 294 84 75 71 69 28 3 3 BseYI C'CCAG_C Cuts at: 0 1174 1386 1567 1982 2180 2316 Size: 1174 212 181 415 198 136 Fragments arranged by size: 1174 415 212 198 181 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 793 2316 Size: 793 1523 BsiEI CG_ry'CG Cuts at: 0 1851 2316 Size: 1851 465 BsiHKAI G_wGCw'C Cuts at: 0 431 975 1133 1522 1925 2203 2316 Size: 431 544 158 389 403 278 113 Fragments arranged by size: 544 431 403 389 278 158 113 BsiWI C'GTAC_G Cuts at: 0 51 2316 Size: 51 2265 BsmI GAATG_Cn' Cuts at: 0 616 2316 Size: 616 1700 Bsp1286I G_dGCh'C Cuts at: 0 195 220 431 475 975 1133 1522 1568 Size: 195 25 211 44 500 158 389 46 Cuts at: 1568 1663 1925 2203 2316 Size: 95 262 278 113 Fragments arranged by size: 500 389 278 262 211 195 158 113 95 46 44 25 BspEI T'CCGG_A Cuts at: 0 92 2316 Size: 92 2224 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 965 2316 Size: 965 1351 BsrBI CCG'CTC Cuts at: 0 381 1967 2316 Size: 381 1586 349 Fragments arranged by size: 1586 381 349 BsrDI GCAATG_nn' Cuts at: 0 1716 2316 Size: 1716 600 BsrFI r'CCGG_y Cuts at: 0 1510 1757 2316 Size: 1510 247 559 Fragments arranged by size: 1510 559 247 BstXI CCAn_nnnn'nTGG Cuts at: 0 1202 1450 2152 2316 Size: 1202 248 702 164 Fragments arranged by size: 1202 702 248 164 BstYI r'GATC_y Cuts at: 0 258 336 1439 2316 Size: 258 78 1103 877 Fragments arranged by size: 1103 877 258 78 Bsu36I CC'TnA_GG Cuts at: 0 405 2316 Size: 405 1911 BtgI C'CryG_G Cuts at: 0 18 326 503 776 2316 Size: 18 308 177 273 1540 Fragments arranged by size: 1540 308 273 177 18 BtsI GCAGTG_nn' Cuts at: 0 545 1992 2316 Size: 545 1447 324 Fragments arranged by size: 1447 545 324 ClaI AT'CG_AT Cuts at: 0 2054 2316 Size: 2054 262 DraIII CAC_nnn'GTG Cuts at: 0 1197 2316 Size: 1197 1119 DrdI GACnn_nn'nnGTC Cuts at: 0 844 2316 Size: 844 1472 EaeI y'GGCC_r Cuts at: 0 1091 1403 1686 1848 2316 Size: 1091 312 283 162 468 Fragments arranged by size: 1091 468 312 283 162 EagI C'GGCC_G Cuts at: 0 1848 2316 Size: 1848 468 EarI CTCTTCn'nnn_ Cuts at: 0 3 442 676 1939 2116 2316 Size: 3 439 234 1263 177 200 Fragments arranged by size: 1263 439 234 200 177 3 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 244 2316 Size: 244 2072 EcoICRI GAG'CTC Cuts at: 0 429 973 2316 Size: 429 544 1343 Fragments arranged by size: 1343 544 429 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 70 236 644 966 1072 1384 1476 2197 Size: 70 166 408 322 106 312 92 721 Cuts at: 2197 2254 2316 Size: 57 62 Fragments arranged by size: 721 408 322 312 166 106 92 70 62 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 593 673 917 2316 Size: 593 80 244 1399 Fragments arranged by size: 1399 593 244 80 EcoO109I rG'GnC_Cy Cuts at: 0 46 191 192 217 270 528 1295 1628 Size: 46 145 1 25 53 258 767 333 Cuts at: 1628 2190 2316 Size: 562 126 Fragments arranged by size: 767 562 333 258 145 126 53 46 25 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 726 1461 2316 Size: 726 735 855 Fragments arranged by size: 855 735 726 FspI TGC'GCA Cuts at: 0 961 2316 Size: 961 1355 HaeII r_GCGC'y Cuts at: 0 422 2316 Size: 422 1894 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 49 81 472 504 999 1031 1518 1550 Size: 49 32 391 32 495 32 487 32 Cuts at: 1550 2316 Size: 766 Fragments arranged by size: 766 495 487 391 49 32 32 32 32 HincII GTy'rAC Cuts at: 0 1062 2316 Size: 1062 1254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 470 710 2316 Size: 470 240 1606 Fragments arranged by size: 1606 470 240 MscI TGG'CCA Cuts at: 0 1093 1405 1688 2316 Size: 1093 312 283 628 Fragments arranged by size: 1093 628 312 283 MslI CAynn'nnrTG Cuts at: 0 815 1448 1466 2316 Size: 815 633 18 850 Fragments arranged by size: 850 815 633 18 MspA1I CmG'CkG Cuts at: 0 114 328 369 944 2316 Size: 114 214 41 575 1372 Fragments arranged by size: 1372 575 214 114 41 NaeI GCC'GGC Cuts at: 0 1512 1759 2316 Size: 1512 247 557 Fragments arranged by size: 1512 557 247 NcoI C'CATG_G Cuts at: 0 18 503 2316 Size: 18 485 1813 Fragments arranged by size: 1813 485 18 NgoMIV G'CCGG_C Cuts at: 0 1510 1757 2316 Size: 1510 247 559 Fragments arranged by size: 1510 559 247 NheI G'CTAG_C Cuts at: 0 550 2316 Size: 550 1766 PasI CC'CwG_GG Cuts at: 0 1026 1582 2316 Size: 1026 556 734 Fragments arranged by size: 1026 734 556 PflMI CCAn_nnn'nTGG Cuts at: 0 340 1197 2316 Size: 340 857 1119 Fragments arranged by size: 1119 857 340 PfoI T'CCnGG_A Cuts at: 0 374 674 2231 2316 Size: 374 300 1557 85 Fragments arranged by size: 1557 374 300 85 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 922 954 2316 Size: 922 32 1362 Fragments arranged by size: 1362 922 32 PpuMI rG'GwC_Cy Cuts at: 0 46 270 528 1628 2190 2316 Size: 46 224 258 1100 562 126 Fragments arranged by size: 1100 562 258 224 126 46 PshAI GACnn'nnGTC Cuts at: 0 2164 2242 2316 Size: 2164 78 74 Fragments arranged by size: 2164 78 74 PspOMI G'GGCC_C Cuts at: 0 191 216 2316 Size: 191 25 2100 Fragments arranged by size: 2100 191 25 PstI C_TGCA'G Cuts at: 0 911 1544 2316 Size: 911 633 772 Fragments arranged by size: 911 772 633 PvuII CAG'CTG Cuts at: 0 369 944 2316 Size: 369 575 1372 Fragments arranged by size: 1372 575 369 SacI G_AGCT'C Cuts at: 0 431 975 2316 Size: 431 544 1341 Fragments arranged by size: 1341 544 431 SacII CC_GC'GG Cuts at: 0 329 2316 Size: 329 1987 SfcI C'TryA_G Cuts at: 0 907 1540 1605 2316 Size: 907 633 65 711 Fragments arranged by size: 907 711 633 65 StuI AGG'CCT Cuts at: 0 1789 2316 Size: 1789 527 StyI C'CwwG_G Cuts at: 0 18 503 1825 2316 Size: 18 485 1322 491 Fragments arranged by size: 1322 491 485 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 187 1117 1208 1508 2316 Size: 187 930 91 300 808 Fragments arranged by size: 930 808 300 187 91 TatI w'GTAC_w Cuts at: 0 761 2316 Size: 761 1555 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2182 2214 2316 Size: 2182 32 102 Fragments arranged by size: 2182 102 32 XmnI GAAnn'nnTTC Cuts at: 0 709 2316 Size: 709 1607 Enzymes that do cut: AcuI AfeI AlfI AloI AlwNI ApaI ApaLI AvaI BaeI BanI BanII BbsI BbvCI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SfcI StuI StyI TaqII TatI TstI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI ApoI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BlpI BmgBI BpuEI BsaI BsaAI BsmBI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraI EcoRI EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NotI NruI NsiI NspI PacI PciI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI