Restriction map for
pFN21AE1600
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# NO CUT ENZYMES ( 41 enzymes) #
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AgeI Asp700I AspI AviII AvrII
BbrPI BcgI BlnI BsaBI BsiWI
Bst1107I BstBI BstZ17I DraI DraIII
FseI FspI HpaI MamI MluI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
SbfI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 5581 GACGTC GACGT^C
AccIII 3889 TCCGGA T^CCGGA
AclI 3334 AACGTT AA^CGTT
AflII 5493 CTTAAG C^TTAAG
Alw44I 3652 GTGCAC G^TGCAC
AocI 4846 CCTGAGG CC^T[ACGT]AGG
ApaLI 3652 GTGCAC G^TGCAC
AscI 262 GGCGCGCC GG^CGCGCC
AseI 4918 ATTAAT AT^TAAT
AsnI 4918 ATTAAT AT^TAAT
BfrI 5493 CTTAAG C^TTAAG
BsaAI 4899 TACGTG [CT]AC^GT[GA]
BseAI 3889 TCCGGA T^CCGGA
BspDI 5575 ATCGAT AT^CGAT
BspEI 3889 TCCGGA T^CCGGA
BspHI 4467 TCATGA T^CATGA
Bsu36I 4846 CCTGAGG CC^T[ACGT]AGG
ClaI 5575 ATCGAT AT^CGAT
Eco81I 4846 CCTGAGG CC^T[ACGT]AGG
HindIII 6426 AAGCTT A^AGCTT
MfeI 1502 CAATTG C^AATTG
MroI 3889 TCCGGA T^CCGGA
MunI 1502 CAATTG C^AATTG
NdeI 4069 CATATG CA^TATG
PaeR7I 5995 CTCGAG C^TCGAG
PshBI 4918 ATTAAT AT^TAAT
Psp1406I 3334 AACGTT AA^CGTT
RcaI 4467 TCATGA T^CATGA
SalI 3163 GTCGAC G^TCGAC
SapI 5542 GAAGAGC GCTCTTC(1/4)
ScaI 5000 AGTACT AGT^ACT
StuI 5853 AGGCCT AGG^CCT
XhoI 5995 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 3163 GTCGAC GT^[AC][GT]AC
6520 GTAGAC
BamHI 1380 GGATCC G^GATCC
3505 GGATCC
BclI 582 TGATCA T^GATCA
5079 TGATCA
BglII 1741 AGATCT A^GATCT
1824 AGATCT
BsaWI 3889 TCCGGA [AT]^CCGG[AT]
5684 ACCGGA
BsmBI 4 GAGACG CGTCTC(1/5)
5723 GAGACG
BsmI 1541 GAATGC GAATGC(1/-1)
2480 GAATGC
BspMI 2969 ACCTGC ACCTGC(4/8)
5503 GCAGGT
BssHII 263 GCGCGC G^CGCGC
1157 GCGCGC
BstEII 3568 GGTGACC G^GT[ACGT]ACC
4719 GGTCACC
BstPI 3568 GGTGACC G^GT[ACGT]ACC
4719 GGTCACC
CpoI 1344 CGGACCG CG^G[AT]CCG
4187 CGGACCG
DrdI 3525 GACCCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4569 GACGCCACCGTC
EcoO65I 3568 GGTGACC G^GT[ACGT]ACC
4719 GGTCACC
EcoRI 5033 GAATTC G^AATTC
6586 GAATTC
FbaI 582 TGATCA T^GATCA
5079 TGATCA
NheI 3004 GCTAGC G^CTAGC
6552 GCTAGC
PpuMI 3782 AGGACCC [GA]G^G[AT]CC[CT]
6156 GGGACCC
RsrII 1344 CGGACCG CG^G[AT]CCG
4187 CGGACCG
SspI 4921 AATATT AAT^ATT
5794 AATATT
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# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI Aor51HI ApaI ApoI Asp718I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRII EcoRV EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NotI
NsiI NspI PflMI PleI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI