(Circular) (Six-base) MAPSORT of: pFN21AE1600.seq Check: 1959 from: 1 to: 11398 pFN21AE1600 11398 bp With 182 enzymes: SgfI * July 13, 2010 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7590 9208 278 Size: 53 83 186 936 6054 1618 2468 Fragments arranged by size: 6054 2468 1618 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5169 8526 8741 1937 Size: 3232 3357 215 4594 Fragments arranged by size: 4594 3357 3232 215 Acc65I G'GTAC_C Cuts at: 2697 3702 6451 8719 2697 Size: 1005 2749 2268 5376 Fragments arranged by size: 5376 2749 2268 1005 AclI AA'CG_TT Cuts at: 1563 5340 9525 9898 1563 Size: 3777 4185 373 3063 Fragments arranged by size: 4185 3777 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5434 5600 7566 7638 9461 10845 11113 1632 Size: 3802 166 1966 72 1823 1384 268 1917 Fragments arranged by size: 3802 1966 1917 1823 1384 268 166 72 AfeI AGC'GCT Cuts at: 2539 3041 6632 2539 Size: 502 3591 7305 Fragments arranged by size: 7305 3591 502 AflII C'TTAA_G Cuts at: 820 1017 7498 820 Size: 197 6481 4720 Fragments arranged by size: 6481 4720 197 AflIII A'CryG_T Cuts at: 1203 3720 5874 6120 8019 10297 11274 1203 Size: 2517 2154 246 1899 2278 977 1327 Fragments arranged by size: 2517 2278 2154 1899 1327 977 246 AgeI A'CCGG_T Cuts at: 10229 10229 Size: 11398 AhdI GACnn_n'nnGTC Cuts at: 4311 5261 7114 10127 4311 Size: 950 1853 3013 5582 Fragments arranged by size: 5582 3013 1853 950 AleI CACnn'nnGTG Cuts at: 1166 5126 1166 Size: 3960 7438 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4665 5762 6377 8102 1184 Size: 3481 1097 615 1725 4480 Fragments arranged by size: 4480 3481 1725 1097 615 AlwNI CAG_nnn'CTG Cuts at: 3679 4820 5395 9141 10713 3679 Size: 1141 575 3746 1572 4364 Fragments arranged by size: 4364 3746 1572 1141 575 ApaI G_GGCC'C Cuts at: 2357 2659 2975 3995 4407 6191 6254 6392 7638 Size: 302 316 1020 412 1784 63 138 1246 Cuts at: 7638 2357 Size: 6117 Fragments arranged by size: 6117 1784 1246 1020 412 316 302 138 63 ApaLI G'TGCA_C Cuts at: 5657 9455 10611 5657 Size: 3798 1156 6444 Fragments arranged by size: 6444 3798 1156 ApoI r'AATT_y Cuts at: 1493 7038 7467 8279 8591 8707 8933 9085 11194 Size: 5545 429 812 312 116 226 152 2109 Cuts at: 11194 1493 Size: 1697 Fragments arranged by size: 5545 2109 1697 812 429 312 226 152 116 AscI GG'CGCG_CC Cuts at: 2268 2268 Size: 11398 AseI AT'TA_AT Cuts at: 160 1032 6924 9952 160 Size: 872 5892 3028 1606 Fragments arranged by size: 5892 3028 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 11398 AvaI C'yCGr_G Cuts at: 1870 1943 2350 2437 2483 2612 2975 3143 3530 Size: 73 407 87 46 129 363 168 387 Cuts at: 3530 3985 4097 4910 5506 6007 6184 6253 8000 Size: 455 112 813 596 501 177 69 1747 Cuts at: 8000 8723 1870 Size: 723 4545 Fragments arranged by size: 4545 1747 813 723 596 501 455 407 387 363 177 168 129 112 87 73 69 46 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3485 3518 3485 Size: 33 11365 BamHI G'GATC_C Cuts at: 3385 5510 8728 9095 3385 Size: 2125 3218 367 5688 Fragments arranged by size: 5688 3218 2125 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 2447 2697 3269 3341 Size: 325 217 627 525 135 250 572 72 Cuts at: 3341 3702 4375 4771 4789 5345 6030 6409 6451 Size: 361 673 396 18 556 685 379 42 Cuts at: 6451 6607 7482 8719 10174 618 Size: 156 875 1237 1455 1842 Fragments arranged by size: 1842 1455 1237 875 685 673 627 572 556 525 396 379 361 325 250 217 156 135 72 42 18 BanII G_rGCy'C Cuts at: 1391 2357 2659 2912 2939 2975 3338 3884 3995 Size: 966 302 253 27 36 363 546 111 Cuts at: 3995 4407 5369 5436 5781 5832 6191 6254 6392 Size: 412 962 67 345 51 359 63 138 Cuts at: 6392 7638 7719 8717 1391 Size: 1246 81 998 4072 Fragments arranged by size: 4072 1246 998 966 962 546 412 363 359 345 302 253 138 111 81 67 63 51 36 27 BbeI G_GCGC'C Cuts at: 2316 2451 3273 4775 4793 6413 6611 7486 2316 Size: 135 822 1502 18 1620 198 875 6228 Fragments arranged by size: 6228 1620 1502 875 822 198 135 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3792 5440 7185 7206 7217 7626 928 Size: 971 1893 1648 1745 21 11 409 4700 Fragments arranged by size: 4700 1893 1745 1648 971 409 21 11 BbvCI CC'TCA_GC Cuts at: 2274 2999 3447 3558 6330 6526 2274 Size: 725 448 111 2772 196 7146 Fragments arranged by size: 7146 2772 725 448 196 111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 9589 9623 1903 Size: 34 7652 34 3678 Fragments arranged by size: 7652 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3513 4076 5997 6234 7679 8231 9290 10500 3513 Size: 563 1921 237 1445 552 1059 1210 4411 Fragments arranged by size: 4411 1921 1445 1210 1059 563 552 237 BclI T'GATC_A Cuts at: 1548 2587 7084 1548 Size: 1039 4497 5862 Fragments arranged by size: 5862 4497 1039 BfrBI ATG'CAT Cuts at: 3962 4264 4438 7785 3962 Size: 302 174 3347 7575 Fragments arranged by size: 7575 3347 302 174 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2565 2654 2742 Size: 107 122 71 631 605 893 89 88 Cuts at: 2742 2864 2979 3020 3293 3302 3489 3615 6114 Size: 122 115 41 273 9 187 126 2499 Cuts at: 6114 6396 10009 136 Size: 282 3613 1525 Fragments arranged by size: 3613 2499 1525 893 631 605 282 273 187 126 122 122 115 107 89 88 71 41 9 BglII A'GATC_T Cuts at: 3746 3829 11393 3746 Size: 83 7564 3751 Fragments arranged by size: 7564 3751 83 BlpI GC'TnA_GC Cuts at: 2366 2933 3883 7776 8815 2366 Size: 567 950 3893 1039 4949 Fragments arranged by size: 4949 3893 1039 950 567 Bme1580I G_kGCm'C Cuts at: 1790 2357 2459 2484 2659 2975 3995 4380 4407 Size: 567 102 25 175 316 1020 385 27 Cuts at: 4407 4476 5661 6033 6191 6254 6392 7638 8126 Size: 69 1185 372 158 63 138 1246 488 Cuts at: 8126 9459 10615 1790 Size: 1333 1156 2573 Fragments arranged by size: 2573 1333 1246 1185 1156 1020 567 488 385 372 316 175 158 138 102 69 63 27 25 BmgBI CAC'GTC Cuts at: 1318 6496 1318 Size: 5178 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3184 3268 3682 Size: 416 53 83 399 24 1767 84 414 Cuts at: 3682 4374 4539 5118 5530 6967 8318 8455 9129 Size: 692 165 579 412 1437 1351 137 674 Cuts at: 9129 10087 442 Size: 958 1753 Fragments arranged by size: 1767 1753 1437 1351 958 692 674 579 416 414 412 399 165 137 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5013 8561 1056 Size: 3957 3548 3893 Fragments arranged by size: 3957 3893 3548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5218 5220 5218 Size: 2 11396 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3471 3961 4336 5468 6081 6301 6586 6659 Size: 2232 490 375 1132 613 220 285 73 Cuts at: 6659 7727 8219 10058 1239 Size: 1068 492 1839 2579 Fragments arranged by size: 2579 2232 1839 1132 1068 613 492 490 375 285 220 73 Bpu10I CC'TnA_GC Cuts at: 2274 2999 3447 3558 6330 6526 2274 Size: 725 448 111 2772 196 7146 Fragments arranged by size: 7146 2772 725 448 196 111 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4153 4282 5197 5335 8029 9523 10388 10686 4153 Size: 129 915 138 2694 1494 865 298 4865 Fragments arranged by size: 4865 2694 1494 915 865 298 138 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3397 7056 8195 10061 882 Size: 617 1898 3659 1139 1866 2219 Fragments arranged by size: 3659 2219 1898 1866 1139 617 BsaAI yAC'GTr Cuts at: 493 1204 6906 9189 493 Size: 711 5702 2283 2702 Fragments arranged by size: 5702 2702 2283 711 BsaBI GATnn'nnATC Cuts at: 11392 11392 Size: 11398 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 2448 3270 4772 Size: 53 83 186 936 780 135 822 1502 Cuts at: 4772 4790 6410 6575 6608 7257 7483 7587 9205 Size: 18 1620 165 33 649 226 104 1618 Cuts at: 9205 9587 10222 275 Size: 382 635 1451 Fragments arranged by size: 1620 1618 1502 1451 936 822 780 649 635 382 226 186 165 135 104 83 53 33 18 BsaWI w'CCGG_w Cuts at: 5894 7689 9831 10229 10503 10650 11061 5894 Size: 1795 2142 398 274 147 411 6231 Fragments arranged by size: 6231 2142 1795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 6760 6790 1178 Size: 30 165 30 1218 30 4109 30 5786 Fragments arranged by size: 5786 4109 1218 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2790 2793 2796 2808 2811 2814 2817 2820 Size: 1604 3 3 12 3 3 3 3 Cuts at: 2820 2823 2826 2841 3747 5008 5086 5444 7157 Size: 3 3 15 906 1261 78 358 1713 Cuts at: 7157 7199 7208 7464 7728 7958 8247 1186 Size: 42 9 256 264 230 289 4337 Fragments arranged by size: 4337 1713 1604 1261 906 358 289 264 256 230 78 42 15 12 9 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2573 3107 3158 3693 4360 4496 5000 5137 5593 Size: 534 51 535 667 136 504 137 456 Cuts at: 5593 6611 6616 7067 10601 11097 2573 Size: 1018 5 451 3534 496 2874 Fragments arranged by size: 3534 2874 1018 667 535 534 504 496 456 451 137 136 51 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5379 7385 7397 8093 8423 8557 1163 Size: 573 3643 2006 12 696 330 134 4004 Fragments arranged by size: 4004 3643 2006 696 573 330 134 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2425 2475 2508 2569 2859 3012 Size: 275 186 424 50 33 61 290 153 Cuts at: 3012 3240 3306 5173 5959 8848 9609 10637 1540 Size: 228 66 1867 786 2889 761 1028 2301 Fragments arranged by size: 2889 2301 1867 1028 786 761 424 290 275 228 186 153 66 61 50 33 BsiHKAI G_wGCw'C Cuts at: 2436 2939 3884 5369 5661 7437 7924 9459 9544 Size: 503 945 1485 292 1776 487 1535 85 Cuts at: 9544 10615 2436 Size: 1071 3219 Fragments arranged by size: 3219 1776 1535 1485 1071 945 503 487 292 85 BsmI GAATG_Cn' Cuts at: 3552 4491 8909 9002 3552 Size: 939 4418 93 5948 Fragments arranged by size: 5948 4418 939 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 7722 2003 Size: 5719 5679 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2357 2436 2459 2484 2659 2912 2939 Size: 399 567 79 23 25 175 253 27 Cuts at: 2939 2975 3338 3884 3995 4380 4407 4476 5369 Size: 36 363 546 111 385 27 69 893 Cuts at: 5369 5436 5661 5781 5832 6033 6191 6254 6392 Size: 67 225 120 51 201 158 63 138 Cuts at: 6392 7437 7638 7719 7924 8126 8717 9459 9544 Size: 1045 201 81 205 202 591 742 85 Cuts at: 9544 10615 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1045 893 742 591 567 546 399 385 363 253 225 205 202 201 201 175 158 138 120 111 85 81 79 69 67 63 51 36 27 27 25 23 BspEI T'CCGG_A Cuts at: 5894 11061 5894 Size: 5167 6231 BspHI T'CATG_A Cuts at: 6472 9287 11017 6472 Size: 2815 1730 6853 Fragments arranged by size: 6853 2815 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4983 7499 8753 844 Size: 4139 2516 1254 3489 Fragments arranged by size: 4139 3489 2516 1254 BsrBI CCG'CTC Cuts at: 1394 1823 2553 2837 8586 9285 1394 Size: 429 730 284 5749 699 3507 Fragments arranged by size: 5749 3507 730 699 429 284 BsrDI GCAATG_nn' Cuts at: 65 1248 5907 6361 7610 7837 9893 10067 65 Size: 1183 4659 454 1249 227 2056 174 1396 Fragments arranged by size: 4659 2056 1396 1249 1183 454 227 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2413 2470 2736 3014 3047 8610 10042 Size: 110 603 57 266 278 33 5563 1432 Cuts at: 10042 10229 1700 Size: 187 2869 Fragments arranged by size: 5563 2869 1432 603 278 266 187 110 57 33 BsrGI T'GTAC_A Cuts at: 96 5877 6123 6159 96 Size: 5781 246 36 5335 Fragments arranged by size: 5781 5335 246 36 BssHII G'CGCG_C Cuts at: 1925 2268 3162 1925 Size: 343 894 10161 Fragments arranged by size: 10161 894 343 BssSI C'ACGA_G Cuts at: 3907 6110 7898 8603 9458 10470 3907 Size: 2203 1788 705 855 1012 4835 Fragments arranged by size: 4835 2203 1788 1012 855 705 BstAPI GCAn_nnn'nTGC Cuts at: 794 3568 3679 3893 794 Size: 2774 111 214 8299 Fragments arranged by size: 8299 2774 214 111 BstBI TT'CG_AA Cuts at: 10202 10202 Size: 11398 BstEII G'GTnAC_C Cuts at: 1184 5573 6724 1184 Size: 4389 1151 5858 Fragments arranged by size: 5858 4389 1151 BstXI CCAn_nnnn'nTGG Cuts at: 4439 4463 5543 6980 4439 Size: 24 1080 1437 8857 Fragments arranged by size: 8857 1437 1080 24 BstYI r'GATC_y Cuts at: 1972 3365 3385 3746 3829 5510 6261 8143 8728 Size: 1393 20 361 83 1681 751 1882 585 Cuts at: 8728 9095 9480 9497 10949 11035 11047 11393 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1882 1681 1452 1393 751 585 385 367 361 346 86 83 20 17 12 Bsu36I CC'TnA_GG Cuts at: 6852 6852 Size: 11398 BtgI C'CryG_G Cuts at: 513 1065 2004 2859 2916 3240 3357 3537 3858 Size: 552 939 855 57 324 117 180 321 Cuts at: 3858 4797 5226 5556 6705 8343 8562 513 Size: 939 429 330 1149 1638 219 3349 Fragments arranged by size: 3349 1638 1149 939 939 855 552 429 330 324 321 219 180 117 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3344 7222 7236 7735 7795 487 Size: 683 56 2118 3878 14 499 60 4090 Fragments arranged by size: 4090 3878 2118 683 499 60 56 14 BtsI GCAGTG_nn' Cuts at: 4641 5362 6584 8914 9708 9728 4641 Size: 721 1222 2330 794 20 6311 Fragments arranged by size: 6311 2330 1222 794 721 20 ClaI AT'CG_AT Cuts at: 1554 7581 1554 Size: 6027 5371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 11363 DraI TTT'AAA Cuts at: 8702 8702 Size: 11398 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5536 6580 10405 809 Size: 507 4220 1044 3825 1802 Fragments arranged by size: 4220 3825 1802 1044 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2335 2422 2505 Size: 54 1425 50 275 18 505 87 83 Cuts at: 2505 2566 2856 3009 3049 3226 3237 3303 3540 Size: 61 290 153 40 177 11 66 237 Cuts at: 3540 5063 5611 7601 8034 8250 8544 8565 8845 Size: 1523 548 1990 433 216 294 21 280 Cuts at: 8845 9734 11363 8 Size: 889 1629 43 Fragments arranged by size: 1990 1629 1523 1425 889 548 505 433 294 290 280 275 237 216 177 153 87 83 66 61 54 50 43 40 21 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2422 2505 2566 2856 3009 3237 3303 Size: 275 610 83 61 290 153 228 66 Cuts at: 3303 8845 1537 Size: 5542 4090 Fragments arranged by size: 5542 4090 610 290 275 228 153 83 66 61 EarI CTCTTCn'nnn_ Cuts at: 1353 3395 5187 7542 9328 1353 Size: 2042 1792 2355 1786 3423 Fragments arranged by size: 3423 2355 2042 1792 1786 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2613 2623 2629 2787 9985 10359 Size: 1064 138 1024 10 6 158 7198 374 Cuts at: 10359 10505 387 Size: 146 1280 Fragments arranged by size: 7198 1280 1064 1024 374 158 146 138 10 6 EcoICRI GAG'CTC Cuts at: 2937 3882 5367 2937 Size: 945 1485 8968 Fragments arranged by size: 8968 1485 945 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3471 3961 4336 5434 5468 5600 6081 Size: 393 1839 490 375 1098 34 132 481 Cuts at: 6081 6301 6586 6659 7566 7638 7727 8219 9461 Size: 220 285 73 907 72 89 492 1242 Cuts at: 9461 10058 10845 11113 1239 Size: 597 787 268 1524 Fragments arranged by size: 1839 1524 1242 1098 907 787 597 492 490 481 393 375 285 268 220 132 89 73 72 34 EcoNI CCTnn'n_nnAGG Cuts at: 3002 6232 6420 3002 Size: 3230 188 7980 Fragments arranged by size: 7980 3230 188 EcoO109I rG'GnC_Cy Cuts at: 2655 2656 2972 3991 4403 5788 5999 6250 6326 Size: 1 316 1019 412 1385 211 251 76 Cuts at: 6326 6388 6389 7635 8162 2655 Size: 62 1 1246 527 5891 Fragments arranged by size: 5891 1385 1246 1019 527 412 316 251 211 76 62 1 1 EcoRI G'AATT_C Cuts at: 7038 8591 8707 9085 7038 Size: 1553 116 378 9351 Fragments arranged by size: 9351 1553 378 116 EcoRV GAT'ATC Cuts at: 6841 8623 8631 6841 Size: 1782 8 9608 Fragments arranged by size: 9608 1782 8 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4323 5809 7678 4323 Size: 1486 1869 8043 Fragments arranged by size: 8043 1869 1486 FspI TGC'GCA Cuts at: 9904 9904 Size: 11398 HaeII r_GCGC'y Cuts at: 1678 2316 2451 2541 3043 3273 4775 4793 5929 Size: 638 135 90 502 230 1502 18 1136 Cuts at: 5929 6109 6413 6611 6634 7486 8351 10545 1678 Size: 180 304 198 23 852 865 2194 2531 Fragments arranged by size: 2531 2194 1502 1136 865 852 638 502 304 230 198 180 135 90 23 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3073 3105 3559 3591 4297 Size: 32 135 32 1501 32 454 32 706 Cuts at: 4297 4329 4826 4858 4904 4936 5247 5279 5654 Size: 32 497 32 46 32 311 32 375 Cuts at: 5654 5686 6454 6486 7100 7132 7577 7609 10039 Size: 32 768 32 614 32 445 32 2430 Cuts at: 10039 10071 10113 10145 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2430 1501 768 706 614 497 454 445 375 311 135 46 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5170 5541 7351 8742 8988 669 Size: 988 281 3232 371 1810 1391 246 3079 Fragments arranged by size: 3232 3079 1810 1391 988 371 281 246 HindIII A'AGCT_T Cuts at: 748 8431 748 Size: 7683 3715 HpaI GTT'AAC Cuts at: 8988 8988 Size: 11398 KasI G'GCGC_C Cuts at: 2312 2447 3269 4771 4789 6409 6607 7482 2312 Size: 135 822 1502 18 1620 198 875 6228 Fragments arranged by size: 6228 1620 1502 875 822 198 135 18 KpnI G_GTAC'C Cuts at: 2701 3706 6455 8723 2701 Size: 1005 2749 2268 5376 Fragments arranged by size: 5376 2749 2268 1005 MfeI C'AATT_G Cuts at: 3507 8997 3507 Size: 5490 5908 MluI A'CGCG_T Cuts at: 11274 11274 Size: 11398 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 3503 5278 8177 10512 10696 1305 Size: 954 1244 1775 2899 2335 184 2007 Fragments arranged by size: 2899 2335 2007 1775 1244 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5065 8036 8252 8567 10 Size: 54 1425 343 3233 2971 216 315 2841 Fragments arranged by size: 3233 2971 2841 1425 343 315 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2094 2115 2966 5031 5126 5186 5744 Size: 648 928 21 851 2065 95 60 558 Cuts at: 5744 7790 8399 8492 9356 9715 9874 518 Size: 2046 609 93 864 359 159 2042 Fragments arranged by size: 2065 2046 2042 928 864 851 648 609 558 359 159 95 93 60 21 MspA1I CmG'CkG Cuts at: 1606 2362 2790 2861 2918 3094 3242 3284 3499 Size: 756 428 71 57 176 148 42 215 Cuts at: 3499 4395 4496 6304 7262 8636 8814 9491 10639 Size: 896 101 1808 958 1374 178 677 1148 Cuts at: 10639 10884 1606 Size: 245 2120 Fragments arranged by size: 2120 1808 1374 1148 958 896 756 677 428 245 215 178 176 148 101 71 57 42 NaeI GCC'GGC Cuts at: 2415 2738 3016 3049 2415 Size: 323 278 33 10764 Fragments arranged by size: 10764 323 278 33 NarI GG'CG_CC Cuts at: 2313 2448 3270 4772 4790 6410 6608 7483 2313 Size: 135 822 1502 18 1620 198 875 6228 Fragments arranged by size: 6228 1620 1502 875 822 198 135 18 NcoI C'CATG_G Cuts at: 513 1065 2004 3357 3537 4797 5556 8343 8562 Size: 552 939 1353 180 1260 759 2787 219 Cuts at: 8562 513 Size: 3349 Fragments arranged by size: 3349 2787 1353 1260 939 759 552 219 180 NdeI CA'TA_TG Cuts at: 387 6075 387 Size: 5688 5710 NgoMIV G'CCGG_C Cuts at: 2413 2736 3014 3047 2413 Size: 323 278 33 10764 Fragments arranged by size: 10764 323 278 33 NheI G'CTAG_C Cuts at: 1052 5009 8557 1052 Size: 3957 3548 3893 Fragments arranged by size: 3957 3893 3548 NotI GC'GGCC_GC Cuts at: 2422 2566 2856 3009 3237 3303 8845 2422 Size: 144 290 153 228 66 5542 4975 Fragments arranged by size: 5542 4975 290 228 153 144 66 NsiI A_TGCA'T Cuts at: 3964 4266 4440 7787 3964 Size: 302 174 3347 7575 Fragments arranged by size: 7575 3347 302 174 NspI r_CATG'y Cuts at: 1132 1229 3724 3962 4268 4561 5749 5869 5878 Size: 97 2495 238 306 293 1188 120 9 Cuts at: 5878 6088 6124 6133 6652 8497 8756 10301 1132 Size: 210 36 9 519 1845 259 1545 2229 Fragments arranged by size: 2495 2229 1845 1545 1188 519 306 293 259 238 210 120 97 36 9 9 PasI CC'CwG_GG Cuts at: 1348 2452 3370 4400 4477 7631 1348 Size: 1104 918 1030 77 3154 5115 Fragments arranged by size: 5115 3154 1104 1030 918 77 PciI A'CATG_T Cuts at: 3720 5874 6120 10297 3720 Size: 2154 246 4177 4821 Fragments arranged by size: 4821 4177 2154 246 PflMI CCAn_nnn'nTGG Cuts at: 1108 4496 4520 7120 1108 Size: 3388 24 2600 5386 Fragments arranged by size: 5386 3388 2600 24 PfoI T'CCnGG_A Cuts at: 2511 4519 8223 2511 Size: 2008 3704 5686 Fragments arranged by size: 5686 3704 2008 PmeI GTTT'AAAC Cuts at: 8702 8702 Size: 11398 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3733 3765 4733 4765 9467 9499 10980 11012 3733 Size: 32 968 32 4702 32 1481 32 4119 Fragments arranged by size: 4702 4119 1481 968 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5788 8162 5788 Size: 2374 9024 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 11398 PsiI TTA'TAA Cuts at: 8968 8968 Size: 11398 PspOMI G'GGCC_C Cuts at: 2353 2655 2971 3991 4403 6187 6250 6388 7634 Size: 302 316 1020 412 1784 63 138 1246 Cuts at: 7634 2353 Size: 6117 Fragments arranged by size: 6117 1784 1246 1020 412 316 302 138 63 PspXI vC'TCGA_Gb Cuts at: 8000 8000 Size: 11398 PstI C_TGCA'G Cuts at: 830 2682 6309 7267 7973 8134 8750 830 Size: 1852 3627 958 706 161 616 3478 Fragments arranged by size: 3627 3478 1852 958 706 616 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 11398 PvuII CAG'CTG Cuts at: 3094 4496 8636 3094 Size: 1402 4140 5856 Fragments arranged by size: 5856 4140 1402 RsrII CG'GwC_CG Cuts at: 3350 6193 3350 Size: 2843 8555 SacI G_AGCT'C Cuts at: 2939 3884 5369 2939 Size: 945 1485 8968 Fragments arranged by size: 8968 1485 945 SacII CC_GC'GG Cuts at: 2862 2919 3243 2862 Size: 57 324 11017 Fragments arranged by size: 11017 324 57 SalI G'TCGA_C Cuts at: 1936 5168 8740 1936 Size: 3232 3572 4594 Fragments arranged by size: 4594 3572 3232 SanDI GG'GwC_CC Cuts at: 8162 8162 Size: 11398 SapI GCTCTTCn'nnn_ Cuts at: 7542 7542 Size: 11398 SbfI CC_TGCA'GG Cuts at: 8750 8750 Size: 11398 ScaI AGT'ACT Cuts at: 7007 9646 11358 7007 Size: 2639 1712 7047 Fragments arranged by size: 7047 2639 1712 SfcI C'TryA_G Cuts at: 826 1045 2147 2678 2731 4020 4390 4726 4780 Size: 219 1102 531 53 1289 370 336 54 Cuts at: 4780 4933 6229 6305 7263 7969 8130 8746 9881 Size: 153 1296 76 958 706 161 616 1135 Cuts at: 9881 10562 10753 826 Size: 681 191 1471 Fragments arranged by size: 1471 1296 1289 1135 1102 958 706 681 616 531 370 336 219 191 161 153 76 54 53 SfiI GGCCn_nnn'nGGCC Cuts at: 2565 3302 3489 6396 2565 Size: 737 187 2907 7567 Fragments arranged by size: 7567 2907 737 187 SfoI GGC'GCC Cuts at: 2314 2449 3271 4773 4791 6411 6609 7484 2314 Size: 135 822 1502 18 1620 198 875 6228 Fragments arranged by size: 6228 1620 1502 875 822 198 135 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 11398 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 11398 SmaI CCC'GGG Cuts at: 1872 2352 2439 2485 2614 2977 3532 3987 4099 Size: 480 87 46 129 363 555 455 112 Cuts at: 4099 6255 8725 1872 Size: 2156 2470 4545 Fragments arranged by size: 4545 2470 2156 555 480 455 363 129 112 87 46 SmlI C'TyrA_G Cuts at: 820 1017 1943 4132 4297 5212 5350 7498 8000 Size: 197 926 2189 165 915 138 2148 502 Cuts at: 8000 8008 9502 10403 10665 820 Size: 8 1494 901 262 1553 Fragments arranged by size: 2189 2148 1553 1494 926 915 901 502 262 197 165 138 8 SnaBI TAC'GTA Cuts at: 493 9189 493 Size: 8696 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 11398 SphI G_CATG'C Cuts at: 1132 4268 5749 5869 8756 1132 Size: 3136 1481 120 2887 3774 Fragments arranged by size: 3774 3136 2887 1481 120 SspI AAT'ATT Cuts at: 5 52 6928 7801 9322 5 Size: 47 6876 873 1521 2081 Fragments arranged by size: 6876 2081 1521 873 47 StuI AGG'CCT Cuts at: 1517 7860 1517 Size: 6343 5055 StyI C'CwwG_G Cuts at: 513 1065 2004 2316 3357 3537 3916 4797 5556 Size: 552 939 312 1041 180 379 881 759 Cuts at: 5556 5995 7119 8343 8368 8547 8562 8837 513 Size: 439 1124 1224 25 179 15 275 3074 Fragments arranged by size: 3074 1224 1124 1041 939 881 759 552 439 379 312 275 180 179 25 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3367 5187 5973 7368 7658 8161 9425 Size: 1255 1787 1820 786 1395 290 503 1264 Cuts at: 9425 9442 9595 9780 10225 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1820 1787 1498 1395 1264 1255 786 503 445 290 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3147 5645 5877 Size: 274 80 33 51 1443 1170 2498 232 Cuts at: 5877 6123 6135 6159 7005 8674 9644 11356 96 Size: 246 12 24 846 1669 970 1712 138 Fragments arranged by size: 2498 1712 1669 1443 1170 970 846 274 246 232 138 80 51 33 24 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2621 2653 6261 6293 6760 6792 11358 11390 2621 Size: 32 3608 32 467 32 4566 32 2629 Fragments arranged by size: 4566 3608 2629 467 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 11398 XbaI T'CTAG_A Cuts at: 8734 8734 Size: 11398 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4040 4265 8569 4040 Size: 225 4304 6869 Fragments arranged by size: 6869 4304 225 XhoI C'TCGA_G Cuts at: 1943 8000 1943 Size: 6057 5341 XmaI C'CCGG_G Cuts at: 1870 2350 2437 2483 2612 2975 3530 3985 4097 Size: 480 87 46 129 363 555 455 112 Cuts at: 4097 6253 8723 1870 Size: 2156 2470 4545 Fragments arranged by size: 4545 2470 2156 555 480 455 363 129 112 87 46 XmnI GAAnn'nnTTC Cuts at: 9527 9527 Size: 11398 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7588 9206 276 Size: 53 83 186 936 6054 1618 2468 Fragments arranged by size: 6054 2468 1618 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BsiWI BstZ17I DraIII FseI FspAI NruI PacI PmlI PsrI SexAI SrfI SwaI