################################## # NO CUT ENZYMES ( 29 enzymes) # ################################## AatII Acc65I AscI Asp718I AviII BbeI BsiWI BssHII Bst1107I BstZ17I CpoI FseI FspI KasI KpnI KspI MluI NarI NotI NruI PacI PmeI PshAI RsrII SacII SfiI SfoI SgrAI SwaI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AclI 246 AACGTT AA^CGTT AcyI 4232 GGCGTC G[GA]^CG[CT]C Alw44I 13804 GTGCAC G^TGCAC ApaLI 13804 GTGCAC G^TGCAC AseI 9636 ATTAAT AT^TAAT AsnI 9636 ATTAAT AT^TAAT AvrII 13638 CCTAGG C^CTAGG BlnI 13638 CCTAGG C^CTAGG BsaHI 4232 GGCGTC G[GA]^CG[CT]C BstBI 6403 TTCGAA TT^CGAA DraIII 252 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 13473 GATATC GAT^ATC Hin1I 4232 GGCGTC G[GA]^CG[CT]C NheI 5143 GCTAGC G^CTAGC NspV 6403 TTCGAA TT^CGAA PshBI 9636 ATTAAT AT^TAAT Psp1406I 246 AACGTT AA^CGTT PvuI 8097 CGATCG CGAT^CG SbfI 5829 CCTGCAGG CCTGCA^GG SfuI 6403 TTCGAA TT^CGAA SnaBI 3488 TACGTA TAC^GTA Sse8387I 5829 CCTGCAGG CCTGCA^GG XbaI 1560 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 2021 TCCGGA T^CCGGA 6794 TCCGGA AflII 4161 CTTAAG C^TTAAG 12639 CTTAAG AgeI 1395 ACCGGT A^CCGGT 15182 ACCGGT Aor51HI 3728 AGCGCT AGC^GCT 15787 AGCGCT ApaI 7546 GGGCCC GGGCC^C 11066 GGGCCC BciVI 13662 GGATAC GTATCC(6/5) 3815 GTATCC BfrI 4161 CTTAAG C^TTAAG 12639 CTTAAG BglI 4715 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 7270 GCCTTCCTGGC BseAI 2021 TCCGGA T^CCGGA 6794 TCCGGA BsmI 4533 GAATGC GAATGC(1/-1) 7575 GAATGC BspDI 5053 ATCGAT AT^CGAT 14935 ATCGAT BspEI 2021 TCCGGA T^CCGGA 6794 TCCGGA ClaI 5053 ATCGAT AT^CGAT 14935 ATCGAT DrdI 12499 GACCCAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 14815 GACTTTGATGTC EagI 1258 CGGCCG C^GGCCG 7504 CGGCCG EclXI 1258 CGGCCG C^GGCCG 7504 CGGCCG Eco47III 3728 AGCGCT AGC^GCT 15787 AGCGCT Eco52I 1258 CGGCCG C^GGCCG 7504 CGGCCG HpaI 2854 GTTAAC GTT^AAC 14212 GTTAAC MroI 2021 TCCGGA T^CCGGA 6794 TCCGGA PaeR7I 3787 CTCGAG C^TCGAG 14867 CTCGAG PinAI 1395 ACCGGT A^CCGGT 15182 ACCGGT SalI 3591 GTCGAC G^TCGAC 9299 GTCGAC SexAI 6638 ACCTGGT A^CC[AT]GGT 8170 ACCTGGT SmaI 374 CCCGGG CCC^GGG 15413 CCCGGG SpeI 456 ACTAGT A^CTAGT 10695 ACTAGT SphI 4118 GCATGC GCATG^C 12423 GCATGC XhoI 3787 CTCGAG C^TCGAG 14867 CTCGAG XmaCI 374 CCCGGG C^CCGGG 15413 CCCGGG XmaI 374 CCCGGG C^CCGGG 15413 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 176 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApoI Asp700I AspEI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbrPI BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NciI NcoI NdeI NdeII NgoMIV NlaIII NlaIV NsiI NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI