(Circular) (Six-base) MAPSORT of: pFN21AE0600.seq Check: 253 from: 1 to: 20605 pFN21AE0600 20605 bp With 182 enzymes: SgfI * May 13, 2010 16:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3457 7420 8209 11608 16723 3457 Size: 3963 789 3399 5115 7339 Fragments arranged by size: 7339 5115 3963 3399 789 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 18415 278 Size: 53 83 186 936 16879 2468 Fragments arranged by size: 16879 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 5597 6320 8422 8917 10953 11305 11943 Size: 790 2870 723 2102 495 2036 352 638 Cuts at: 11943 12446 13512 17948 1937 Size: 503 1066 4436 4594 Fragments arranged by size: 4594 4436 2870 2102 2036 1066 790 723 638 503 495 352 Acc65I G'GTAC_C Cuts at: 17926 17926 Size: 20605 AclI AA'CG_TT Cuts at: 1563 2252 18732 19105 1563 Size: 689 16480 373 3063 Fragments arranged by size: 16480 3063 689 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3524 3765 4025 4363 4967 6120 6471 6575 Size: 1892 241 260 338 604 1153 351 104 Cuts at: 6575 6584 7068 7427 7445 8862 8930 9104 10064 Size: 9 484 359 18 1417 68 174 960 Cuts at: 10064 10341 10656 11313 11585 12857 12919 13527 13737 Size: 277 315 657 272 1272 62 608 210 Cuts at: 13737 14004 14090 14705 15491 15923 16119 16707 17766 Size: 267 86 615 786 432 196 588 1059 Cuts at: 17766 18668 20052 20320 1632 Size: 902 1384 268 1917 Fragments arranged by size: 1917 1892 1417 1384 1272 1153 1059 960 902 786 657 615 608 604 588 484 432 359 351 338 315 277 272 268 267 260 241 210 196 174 104 86 68 62 18 9 AfeI AGC'GCT Cuts at: 5735 17794 5735 Size: 12059 8546 AflII C'TTAA_G Cuts at: 820 1017 6166 14644 820 Size: 197 5149 8478 6781 Fragments arranged by size: 8478 6781 5149 197 AflIII A'CryG_T Cuts at: 1203 6283 6953 6955 9054 9573 12962 13341 16925 Size: 5080 670 2 2099 519 3389 379 3584 Cuts at: 16925 19504 20481 1203 Size: 2579 977 1327 Fragments arranged by size: 5080 3584 3389 2579 2099 1327 977 670 519 379 2 AgeI A'CCGG_T Cuts at: 3400 17187 19436 3400 Size: 13787 2249 4569 Fragments arranged by size: 13787 4569 2249 AhdI GACnn_n'nnGTC Cuts at: 2514 2801 7646 13905 14959 17892 19334 2514 Size: 287 4845 6259 1054 2933 1442 3785 Fragments arranged by size: 6259 4845 3785 2933 1442 1054 287 AleI CACnn'nnGTG Cuts at: 1166 2870 12945 14268 1166 Size: 1704 10075 1323 7503 Fragments arranged by size: 10075 7503 1704 1323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 3390 3854 5280 6555 8304 8791 10228 Size: 1206 1000 464 1426 1275 1749 487 1437 Cuts at: 10228 14951 1184 Size: 4723 6838 Fragments arranged by size: 6838 4723 1749 1437 1426 1275 1206 1000 487 464 AlwNI CAG_nnn'CTG Cuts at: 3484 4048 5042 5098 6412 6670 7111 7357 7369 Size: 564 994 56 1314 258 441 246 12 Cuts at: 7369 7889 9011 9181 9683 9826 9908 9991 10981 Size: 520 1122 170 502 143 82 83 990 Cuts at: 10981 12182 13122 15901 15976 18348 19920 3484 Size: 1201 940 2779 75 2372 1572 4169 Fragments arranged by size: 4169 2779 2372 1572 1314 1201 1122 994 990 940 564 520 502 441 258 246 170 143 83 82 75 56 12 ApaI G_GGCC'C Cuts at: 9555 13075 9555 Size: 3520 17085 ApaLI G'TGCA_C Cuts at: 15809 18662 19818 15809 Size: 2853 1156 16596 Fragments arranged by size: 16596 2853 1156 ApoI r'AATT_y Cuts at: 1493 3093 3161 3949 4055 5214 5635 6017 7276 Size: 1600 68 788 106 1159 421 382 1259 Cuts at: 7276 7460 8795 9298 9309 9387 9617 9939 11259 Size: 184 1335 503 11 78 230 322 1320 Cuts at: 11259 13733 15077 15085 15098 15307 16181 16805 17042 Size: 2474 1344 8 13 209 874 624 237 Cuts at: 17042 17279 17342 17914 18140 18292 20401 1493 Size: 237 63 572 226 152 2109 1697 Fragments arranged by size: 2474 2109 1697 1600 1344 1335 1320 1259 1159 874 788 624 572 503 421 382 322 237 237 230 226 209 184 152 106 78 68 63 13 11 8 AseI AT'TA_AT Cuts at: 160 1032 11642 19159 160 Size: 872 10610 7517 1606 Fragments arranged by size: 10610 7517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 20605 AvaI C'yCGr_G Cuts at: 1870 1943 2379 3714 5792 7141 7547 13137 13710 Size: 73 436 1335 2078 1349 406 5590 573 Cuts at: 13710 16872 17418 17930 1870 Size: 3162 546 512 4545 Fragments arranged by size: 5590 4545 3162 2078 1349 1335 573 546 512 436 406 73 AvrII C'CTAG_G Cuts at: 15643 15643 Size: 20605 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 12524 12557 12524 Size: 33 20572 BamHI G'GATC_C Cuts at: 10555 11593 13935 16419 17935 18302 10555 Size: 1038 2342 2484 1516 367 12858 Fragments arranged by size: 12858 2484 2342 1516 1038 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4035 4747 5772 12637 17630 Size: 325 217 627 2248 712 1025 6865 4993 Cuts at: 17630 17926 19381 618 Size: 296 1455 1842 Fragments arranged by size: 6865 4993 2248 1842 1455 1025 712 627 325 296 217 BanII G_rGCy'C Cuts at: 1391 3905 6881 8447 8595 8663 9555 10395 10918 Size: 2514 2976 1566 148 68 892 840 523 Cuts at: 10918 11838 13075 13116 13381 14404 14529 16312 16398 Size: 920 1237 41 265 1023 125 1783 86 Cuts at: 16398 17924 1391 Size: 1526 4072 Fragments arranged by size: 4072 2976 2514 1783 1566 1526 1237 1023 920 892 840 523 265 148 125 86 68 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 2868 3530 4485 5004 8616 8747 Size: 971 102 867 662 955 519 3612 131 Cuts at: 8747 10095 10500 12093 15542 17870 928 Size: 1348 405 1593 3449 2328 3663 Fragments arranged by size: 3663 3612 3449 2328 1593 1348 971 955 867 662 519 405 131 102 BbvCI CC'TCA_GC Cuts at: 4945 11034 12473 15795 4945 Size: 6089 1439 3322 9755 Fragments arranged by size: 9755 6089 3322 1439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4997 5031 5681 5715 12203 12237 16097 Size: 34 3060 34 650 34 6488 34 3860 Cuts at: 16097 16131 18796 18830 1903 Size: 34 2665 34 3678 Fragments arranged by size: 6488 3860 3678 3060 2665 650 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5831 15661 18497 19707 5831 Size: 9830 2836 1210 6729 Fragments arranged by size: 9830 6729 2836 1210 BclI T'GATC_A Cuts at: 1548 4719 10662 13174 15300 1548 Size: 3171 5943 2512 2126 6853 Fragments arranged by size: 6853 5943 3171 2512 2126 BfrBI ATG'CAT Cuts at: 5267 7528 8768 5267 Size: 2261 1240 17104 Fragments arranged by size: 17104 2261 1240 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6726 9281 19216 Size: 107 122 71 631 605 5054 2555 9935 Cuts at: 19216 136 Size: 1525 Fragments arranged by size: 9935 5054 2555 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2317 2547 5569 6471 9031 10857 20600 2317 Size: 230 3022 902 2560 1826 9743 2322 Fragments arranged by size: 9743 3022 2560 2322 1826 902 230 BlpI GC'TnA_GC Cuts at: 5951 6875 7959 8106 11810 12126 12867 13115 16338 Size: 924 1084 147 3704 316 741 248 3223 Cuts at: 16338 16392 16503 18022 5951 Size: 54 111 1519 8534 Fragments arranged by size: 8534 3704 3223 1519 1084 924 741 316 248 147 111 54 Bme1580I G_kGCm'C Cuts at: 1790 9555 12642 13075 13144 15813 18666 19822 1790 Size: 7765 3087 433 69 2669 2853 1156 2573 Fragments arranged by size: 7765 3087 2853 2669 2573 1156 433 69 BmgBI CAC'GTC Cuts at: 1318 11266 16549 16928 1318 Size: 9948 5283 379 4995 Fragments arranged by size: 9948 5283 4995 379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2807 4297 7288 Size: 416 53 83 399 24 1390 1490 2991 Cuts at: 7288 8938 9808 11971 12532 13399 15264 15881 18336 Size: 1650 870 2163 561 867 1865 617 2455 Cuts at: 18336 19294 442 Size: 958 1753 Fragments arranged by size: 2991 2455 2163 1865 1753 1650 1490 1390 958 870 867 617 561 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 7152 1056 Size: 6096 14509 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2124 2377 3919 7156 9181 11167 12639 2124 Size: 253 1542 3237 2025 1986 1472 10090 Fragments arranged by size: 10090 3237 2025 1986 1542 1472 253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2692 2829 2929 3461 3833 4007 4147 4211 Size: 1453 137 100 532 372 174 140 64 Cuts at: 4211 4418 4472 5204 5603 6263 6692 6926 6995 Size: 207 54 732 399 660 429 234 69 Cuts at: 6995 7538 7898 7926 8186 9705 9710 9815 10103 Size: 543 360 28 260 1519 5 105 288 Cuts at: 10103 10374 10922 11129 11204 11498 11894 12041 12664 Size: 271 548 207 75 294 396 147 623 Cuts at: 12664 13149 13481 13943 14646 15953 16772 16811 16852 Size: 485 332 462 703 1307 819 39 41 Cuts at: 16852 17431 17478 17800 17874 19265 1239 Size: 579 47 322 74 1391 2579 Fragments arranged by size: 2579 1519 1453 1391 1307 819 732 703 660 623 579 548 543 532 485 462 429 399 396 372 360 332 322 294 288 271 260 234 207 207 174 147 140 137 105 100 75 74 69 64 54 47 41 39 28 5 Bpu10I CC'TnA_GC Cuts at: 4945 6921 7302 8257 9663 10790 11034 12473 13187 Size: 1976 381 955 1406 1127 244 1439 714 Cuts at: 13187 15275 15795 4945 Size: 2088 520 9755 Fragments arranged by size: 9755 2088 1976 1439 1406 1127 955 714 520 381 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3168 3889 4268 4429 6135 7639 8657 10016 10433 Size: 721 379 161 1706 1504 1018 1359 417 Cuts at: 10433 10863 10874 14084 16052 18730 19595 19893 3168 Size: 430 11 3210 1968 2678 865 298 3880 Fragments arranged by size: 3880 3210 2678 1968 1706 1504 1359 1018 865 721 430 417 379 298 161 11 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3455 5450 6356 7544 9923 11351 14482 Size: 617 1956 1995 906 1188 2379 1428 3131 Cuts at: 14482 14496 15222 16427 19268 882 Size: 14 726 1205 2841 2219 Fragments arranged by size: 3131 2841 2379 2219 1995 1956 1428 1205 1188 906 726 617 14 BsaAI yAC'GTr Cuts at: 493 1204 5495 6211 6884 6956 18396 493 Size: 711 4291 716 673 72 11440 2702 Fragments arranged by size: 11440 4291 2702 716 711 673 72 BsaBI GATnn'nnATC Cuts at: 7774 16298 17076 20599 7774 Size: 8524 778 3523 7780 Fragments arranged by size: 8524 7780 3523 778 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6238 18412 18794 19429 Size: 53 83 186 936 4705 12174 382 635 Cuts at: 19429 275 Size: 1451 Fragments arranged by size: 12174 4705 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3400 3585 4026 8799 17187 19038 19436 19710 19857 Size: 185 441 4773 8388 1851 398 274 147 Cuts at: 19857 20268 3400 Size: 411 3737 Fragments arranged by size: 8388 4773 3737 1851 441 411 398 274 185 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2648 2678 3908 3938 3980 Size: 30 165 30 1245 30 1230 30 42 Cuts at: 3980 4010 8159 8189 9032 9062 10283 10313 14672 Size: 30 4149 30 843 30 1221 30 4359 Cuts at: 14672 14702 14990 15020 16645 16675 1178 Size: 30 288 30 1625 30 5108 Fragments arranged by size: 5108 4359 4149 1625 1245 1230 1221 843 288 165 42 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3730 4118 4121 4208 5078 5144 5201 6260 Size: 2544 388 3 87 870 66 57 1059 Cuts at: 6260 6263 6266 6284 7358 7868 9158 11513 11846 Size: 3 3 18 1074 510 1290 2355 333 Cuts at: 11846 12335 12500 12503 12986 13376 13622 14717 16334 Size: 489 165 3 483 390 246 1095 1617 Cuts at: 16334 16538 16650 16808 1186 Size: 204 112 158 4983 Fragments arranged by size: 4983 2544 2355 1617 1290 1095 1074 1059 870 510 489 483 390 388 333 246 204 165 158 112 87 66 57 18 3 3 3 3 BseYI C'CCAG_C Cuts at: 3898 4258 5754 5787 5892 6024 6312 6738 7266 Size: 360 1496 33 105 132 288 426 528 Cuts at: 7266 7369 9501 9547 10140 10709 11043 14676 14880 Size: 103 2132 46 593 569 334 3633 204 Cuts at: 14880 15987 17503 17687 19808 20304 3898 Size: 1107 1516 184 2121 496 4199 Fragments arranged by size: 4199 3633 2132 2121 1516 1496 1107 593 569 528 496 426 360 334 288 204 184 132 105 103 46 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 7454 10583 11673 14108 14134 15344 Size: 573 705 5013 3129 1090 2435 26 1210 Cuts at: 15344 1163 Size: 6424 Fragments arranged by size: 6424 5013 3129 2435 1210 1090 705 573 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2096 3266 9512 10105 17011 18055 Size: 275 186 95 1170 6246 593 6906 1044 Cuts at: 18055 18816 19844 1540 Size: 761 1028 2301 Fragments arranged by size: 6906 6246 2301 1170 1044 1028 761 593 275 186 95 BsiHKAI G_wGCw'C Cuts at: 5292 6712 7965 8447 8595 8643 8663 9812 10014 Size: 1420 1253 482 148 48 20 1149 202 Cuts at: 10014 10395 10918 11838 12585 12625 13116 14529 15195 Size: 381 523 920 747 40 491 1413 666 Cuts at: 15195 15592 15813 15983 16132 16312 16398 17311 18666 Size: 397 221 170 149 180 86 913 1355 Cuts at: 18666 18751 19822 5292 Size: 85 1071 6075 Fragments arranged by size: 6075 1420 1413 1355 1253 1149 1071 920 913 747 666 523 491 482 397 381 221 202 180 170 149 148 86 85 48 40 20 BsmI GAATG_Cn' Cuts at: 6544 9586 18116 18209 6544 Size: 3042 8530 93 8940 Fragments arranged by size: 8940 8530 3042 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2018 14294 17750 2018 Size: 12276 3456 4873 Fragments arranged by size: 12276 4873 3456 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3905 5292 6712 6881 7965 8447 8595 Size: 399 2115 1387 1420 169 1084 482 148 Cuts at: 8595 8643 8663 9555 9812 10014 10395 10918 11838 Size: 48 20 892 257 202 381 523 920 Cuts at: 11838 12585 12625 12642 13075 13116 13144 13381 14404 Size: 747 40 17 433 41 28 237 1023 Cuts at: 14404 14529 15195 15592 15813 15983 16132 16312 16398 Size: 125 666 397 221 170 149 180 86 Cuts at: 16398 17311 17924 18666 18751 19822 1391 Size: 913 613 742 85 1071 2174 Fragments arranged by size: 2174 2115 1420 1387 1084 1071 1023 920 913 892 747 742 666 613 523 482 433 399 397 381 257 237 221 202 180 170 169 149 148 125 86 85 48 41 40 28 20 17 BspEI T'CCGG_A Cuts at: 4026 8799 20268 4026 Size: 4773 11469 4363 Fragments arranged by size: 11469 4773 4363 BspHI T'CATG_A Cuts at: 9100 13177 13429 15831 16185 18494 20224 9100 Size: 4077 252 2402 354 2309 1730 9481 Fragments arranged by size: 9481 4077 2402 2309 1730 354 252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 3457 3846 4491 6397 7420 8209 11608 Size: 1307 1306 389 645 1906 1023 789 3399 Cuts at: 11608 16723 17960 844 Size: 5115 1237 3489 Fragments arranged by size: 5115 3489 3399 1906 1307 1306 1237 1023 789 645 389 BsrBI CCG'CTC Cuts at: 1394 1823 2038 2056 2092 10237 10623 13069 15694 Size: 429 215 18 36 8145 386 2446 2625 Cuts at: 15694 16756 17388 18492 1394 Size: 1062 632 1104 3507 Fragments arranged by size: 8145 3507 2625 2446 1104 1062 632 429 386 215 36 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 3838 4749 5781 7232 8286 11804 Size: 1183 1234 1356 911 1032 1451 1054 3518 Cuts at: 11804 14608 19100 19274 65 Size: 2804 4492 174 1396 Fragments arranged by size: 4492 3518 2804 1451 1396 1356 1234 1183 1054 1032 911 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3265 3400 16026 17187 17718 19249 19436 Size: 110 1455 135 12626 1161 531 1531 187 Cuts at: 19436 1700 Size: 2869 Fragments arranged by size: 12626 2869 1531 1455 1161 531 187 135 110 BsrGI T'GTAC_A Cuts at: 96 3429 3572 4912 5148 7763 11671 17474 96 Size: 3333 143 1340 236 2615 3908 5803 3227 Fragments arranged by size: 5803 3908 3333 3227 2615 1340 236 143 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 20605 BssSI C'ACGA_G Cuts at: 4389 7998 9968 17310 18665 19677 4389 Size: 3609 1970 7342 1355 1012 5317 Fragments arranged by size: 7342 5317 3609 1970 1355 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2204 2419 4852 7354 10130 794 Size: 1410 215 2433 2502 2776 11269 Fragments arranged by size: 11269 2776 2502 2433 1410 215 BstBI TT'CG_AA Cuts at: 8409 19409 8409 Size: 11000 9605 BstEII G'GTnAC_C Cuts at: 1184 3553 8329 16558 1184 Size: 2369 4776 8229 5231 Fragments arranged by size: 8229 5231 4776 2369 BstXI CCAn_nnnn'nTGG Cuts at: 4798 5441 5683 4798 Size: 643 242 19720 Fragments arranged by size: 19720 643 242 BstYI r'GATC_y Cuts at: 1972 2317 2547 3472 3480 5569 6471 6676 6745 Size: 345 230 925 8 2089 902 205 69 Cuts at: 6745 7775 8583 9031 9042 10555 10857 11003 11593 Size: 1030 808 448 11 1513 302 146 590 Cuts at: 11593 11889 13642 13935 16419 17935 18302 18687 18704 Size: 296 1753 293 2484 1516 367 385 17 Cuts at: 18704 20156 20242 20254 20600 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2484 2089 1977 1753 1516 1513 1452 1030 925 902 808 590 448 385 367 346 345 302 296 293 230 205 146 86 69 17 12 11 8 Bsu36I CC'TnA_GG Cuts at: 2907 7341 10947 13296 2907 Size: 4434 3606 2349 10216 Fragments arranged by size: 10216 4434 3606 2349 BtgI C'CryG_G Cuts at: 513 1065 4497 5109 5854 9210 9512 10505 13554 Size: 552 3432 612 745 3356 302 993 3049 Cuts at: 13554 13600 16233 16700 513 Size: 46 2633 467 4418 Fragments arranged by size: 4418 3432 3356 3049 2633 993 745 612 552 467 302 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 10011 487 Size: 683 56 8785 11081 Fragments arranged by size: 11081 8785 683 56 BtsI GCAGTG_nn' Cuts at: 2147 3362 4316 5886 6990 7743 8376 9182 11128 Size: 1215 954 1570 1104 753 633 806 1946 Cuts at: 11128 17871 18121 18915 18935 2147 Size: 6743 250 794 20 3817 Fragments arranged by size: 6743 3817 1946 1570 1215 1104 954 806 794 753 633 250 20 ClaI AT'CG_AT Cuts at: 1554 7059 16941 1554 Size: 5505 9882 5218 Fragments arranged by size: 9882 5505 5218 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6802 6837 6870 6905 11362 11397 16250 Size: 35 6238 35 33 35 4457 35 4853 Cuts at: 16250 16285 17109 17144 529 Size: 35 824 35 3990 Fragments arranged by size: 6238 4853 4457 3990 824 35 35 35 35 35 35 33 DraI TTT'AAA Cuts at: 11569 12237 15135 17909 11569 Size: 668 2898 2774 14265 Fragments arranged by size: 14265 2898 2774 668 DraIII CAC_nnn'GTG Cuts at: 2262 2262 Size: 20605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 14510 16826 19612 809 Size: 507 13194 2316 2786 1802 Fragments arranged by size: 13194 2786 2316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 3099 3166 Size: 54 1425 50 275 18 499 770 67 Cuts at: 3166 3263 3729 4800 5139 6480 7287 7324 9509 Size: 97 466 1071 339 1341 807 37 2185 Cuts at: 9509 11119 11632 12102 12429 18052 18941 20570 8 Size: 1610 513 470 327 5623 889 1629 43 Fragments arranged by size: 5623 2185 1629 1610 1425 1341 1071 889 807 770 513 499 470 466 339 327 275 97 67 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 3263 9509 18052 1537 Size: 275 1451 6246 8543 4090 Fragments arranged by size: 8543 6246 4090 1451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2016 3207 3507 3789 3888 5055 6139 6261 Size: 663 1191 300 282 99 1167 1084 122 Cuts at: 6261 6414 6852 6903 6942 8906 9921 10260 10353 Size: 153 438 51 39 1964 1015 339 93 Cuts at: 10353 10404 10737 11316 11604 11691 12303 12938 13104 Size: 51 333 579 288 87 612 635 166 Cuts at: 13104 13890 13911 13988 14109 14463 14892 15048 15183 Size: 786 21 77 121 354 429 156 135 Cuts at: 15183 17061 18535 1353 Size: 1878 1474 3423 Fragments arranged by size: 3423 1964 1878 1474 1191 1167 1084 1015 786 663 635 612 579 438 429 354 339 333 300 288 282 166 156 153 135 122 121 99 93 87 77 51 51 39 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6982 19192 19566 19712 387 Size: 1064 138 5393 12210 374 146 1280 Fragments arranged by size: 12210 5393 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 8445 8593 8661 10393 10916 11836 13114 14527 16310 Size: 148 68 1732 523 920 1278 1413 1783 Cuts at: 16310 16396 8445 Size: 86 12654 Fragments arranged by size: 12654 1783 1732 1413 1278 920 523 148 86 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2692 2829 2929 3461 3524 3765 3833 Size: 393 1060 137 100 532 63 241 68 Cuts at: 3833 4007 4025 4147 4211 4363 4418 4472 4967 Size: 174 18 122 64 152 55 54 495 Cuts at: 4967 5204 5603 6120 6263 6471 6575 6584 6692 Size: 237 399 517 143 208 104 9 108 Cuts at: 6692 6926 6995 7068 7427 7445 7538 7898 7926 Size: 234 69 73 359 18 93 360 28 Cuts at: 7926 8186 8862 8930 9104 9705 9710 9815 10064 Size: 260 676 68 174 601 5 105 249 Cuts at: 10064 10103 10341 10374 10656 10922 11129 11204 11313 Size: 39 238 33 282 266 207 75 109 Cuts at: 11313 11498 11585 11894 12041 12664 12857 12919 13149 Size: 185 87 309 147 623 193 62 230 Cuts at: 13149 13481 13527 13737 13943 14004 14090 14646 14705 Size: 332 46 210 206 61 86 556 59 Cuts at: 14705 15491 15923 15953 16119 16707 16772 16811 16852 Size: 786 432 30 166 588 65 39 41 Cuts at: 16852 17431 17478 17766 17800 17874 18668 19265 20052 Size: 579 47 288 34 74 794 597 787 Cuts at: 20052 20320 1239 Size: 268 1524 Fragments arranged by size: 1524 1060 794 787 786 676 623 601 597 588 579 556 532 517 495 432 399 393 360 359 332 309 288 282 268 266 260 249 241 238 237 234 230 210 208 207 206 193 185 174 174 166 152 147 143 137 122 109 108 105 104 100 93 87 86 75 74 73 69 68 68 65 64 63 62 61 59 55 54 47 46 41 39 39 34 33 30 28 18 18 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 4487 5657 6548 10077 12848 13299 17354 17881 4487 Size: 1170 891 3529 2771 451 4055 527 7211 Fragments arranged by size: 7211 4055 3529 2771 1170 891 527 451 EcoO109I rG'GnC_Cy Cuts at: 2356 8814 9551 9552 9680 10173 10936 11509 12397 Size: 6458 737 1 128 493 763 573 888 Cuts at: 12397 15058 15817 15898 16150 16324 17894 2356 Size: 2661 759 81 252 174 1570 5067 Fragments arranged by size: 6458 5067 2661 1570 888 763 759 737 573 493 252 174 128 81 1 EcoRI G'AATT_C Cuts at: 5214 5635 8795 9298 15077 15085 16181 17914 18292 Size: 421 3160 503 5779 8 1096 1733 378 Cuts at: 18292 5214 Size: 7527 Fragments arranged by size: 7527 5779 3160 1733 1096 503 421 378 8 EcoRV GAT'ATC Cuts at: 15480 15480 Size: 20605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 8323 17751 8323 Size: 9428 11177 FspI TGC'GCA Cuts at: 19111 19111 Size: 20605 HaeII r_GCGC'y Cuts at: 1678 5737 6118 6941 10621 13600 17796 19752 1678 Size: 4059 381 823 3680 2979 4196 1956 2531 Fragments arranged by size: 4196 4059 3680 2979 2531 1956 823 381 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2500 2532 2787 2819 2825 Size: 32 135 32 928 32 255 32 6 Cuts at: 2825 2840 2857 2872 2907 2939 4385 4417 5686 Size: 15 17 15 35 32 1446 32 1269 Cuts at: 5686 5718 7048 7080 7632 7664 8756 8788 9206 Size: 32 1330 32 552 32 1092 32 418 Cuts at: 9206 9238 9721 9753 9989 10021 10424 10456 11137 Size: 32 483 32 236 32 403 32 681 Cuts at: 11137 11169 11692 11724 11991 12023 12131 12163 12850 Size: 32 523 32 267 32 108 32 687 Cuts at: 12850 12882 12991 13023 13515 13547 13891 13923 14893 Size: 32 109 32 492 32 344 32 970 Cuts at: 14893 14925 14945 14977 15219 15251 16056 16088 16205 Size: 32 20 32 242 32 805 32 117 Cuts at: 16205 16230 16237 16262 16912 16944 17348 17380 17747 Size: 25 7 25 650 32 404 32 367 Cuts at: 17747 17779 17878 17910 19246 19278 19320 19352 1373 Size: 32 99 32 1336 32 42 32 2626 Fragments arranged by size: 2626 1446 1336 1330 1269 1092 970 928 805 687 681 650 552 523 492 483 418 404 403 367 344 267 255 242 236 135 117 109 108 99 42 35 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 25 25 20 17 15 15 7 6 HincII GTy'rAC Cuts at: 669 1657 1938 2269 2508 4179 4861 5598 6891 Size: 988 281 331 239 1671 682 737 1293 Cuts at: 6891 11306 14590 14727 15763 16219 17949 18195 669 Size: 4415 3284 137 1036 456 1730 246 3079 Fragments arranged by size: 4415 3284 3079 1730 1671 1293 1036 988 737 682 456 331 281 246 239 137 HindIII A'AGCT_T Cuts at: 748 4934 5073 6908 7582 8456 10807 11763 12164 Size: 4186 139 1835 674 874 2351 956 401 Cuts at: 12164 14255 15414 748 Size: 2091 1159 5939 Fragments arranged by size: 5939 4186 2351 2091 1835 1159 956 874 674 401 139 HpaI GTT'AAC Cuts at: 4861 16219 18195 4861 Size: 11358 1976 7271 Fragments arranged by size: 11358 7271 1976 KpnI G_GTAC'C Cuts at: 17930 17930 Size: 20605 MfeI C'AATT_G Cuts at: 5312 6533 8133 8962 9920 11156 18204 5312 Size: 1221 1600 829 958 1236 7048 7713 Fragments arranged by size: 7713 7048 1600 1236 1221 958 829 MluI A'CGCG_T Cuts at: 20481 20481 Size: 20605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2027 2045 2331 7476 8507 8602 8769 9254 Size: 722 18 286 5145 1031 95 167 485 Cuts at: 9254 9270 9841 10161 12576 13431 15687 16065 16832 Size: 16 571 320 2415 855 2256 378 767 Cuts at: 16832 17558 17799 19719 19903 1305 Size: 726 241 1920 184 2007 Fragments arranged by size: 5145 2415 2256 2007 1920 1031 855 767 726 722 571 485 378 320 286 241 184 167 95 18 16 MscI TGG'CCA Cuts at: 10 64 1489 1832 2331 3101 3731 4802 5141 Size: 54 1425 343 499 770 630 1071 339 Cuts at: 5141 6482 7289 7326 11121 11634 12104 12431 10 Size: 1341 807 37 3795 513 470 327 8184 Fragments arranged by size: 8184 3795 1425 1341 1071 807 770 630 513 499 470 343 339 327 54 37 MslI CAynn'nnrTG Cuts at: 518 1166 2301 2870 3198 3236 3885 4796 5439 Size: 648 1135 569 328 38 649 911 643 Cuts at: 5439 7193 8043 8267 12945 14268 17240 18563 18922 Size: 1754 850 224 4678 1323 2972 1323 359 Cuts at: 18922 19081 518 Size: 159 2042 Fragments arranged by size: 4678 2972 2042 1754 1323 1323 1135 911 850 649 648 643 569 359 328 224 159 38 MspA1I CmG'CkG Cuts at: 1606 2630 3463 4076 4324 4702 4852 5758 7108 Size: 1024 833 613 248 378 150 906 1350 Cuts at: 7108 8404 11422 11814 11914 12019 12082 12125 12427 Size: 1296 3018 392 100 105 63 43 302 Cuts at: 12427 14884 15790 16450 16626 16725 17472 18021 18698 Size: 2457 906 660 176 99 747 549 677 Cuts at: 18698 19846 20091 1606 Size: 1148 245 2120 Fragments arranged by size: 3018 2457 2120 1350 1296 1148 1024 906 906 833 747 677 660 613 549 392 378 302 248 245 176 150 105 100 99 63 43 NaeI GCC'GGC Cuts at: 3267 16028 17720 3267 Size: 12761 1692 6152 Fragments arranged by size: 12761 6152 1692 NcoI C'CATG_G Cuts at: 513 1065 4497 5109 5854 9210 10505 16233 513 Size: 552 3432 612 745 3356 1295 5728 4885 Fragments arranged by size: 5728 4885 3432 3356 1295 745 612 552 NdeI CA'TA_TG Cuts at: 387 4120 11310 14865 387 Size: 3733 7190 3555 6127 Fragments arranged by size: 7190 6127 3733 3555 NgoMIV G'CCGG_C Cuts at: 3265 16026 17718 3265 Size: 12761 1692 6152 Fragments arranged by size: 12761 6152 1692 NheI G'CTAG_C Cuts at: 1052 7148 1052 Size: 6096 14509 NotI GC'GGCC_GC Cuts at: 18052 18052 Size: 20605 NsiI A_TGCA'T Cuts at: 5269 7530 8770 5269 Size: 2261 1240 17104 Fragments arranged by size: 17104 2261 1240 NspI r_CATG'y Cuts at: 1132 1229 4349 4859 6127 6287 7528 8048 9058 Size: 97 3120 510 1268 160 1241 520 1010 Cuts at: 9058 9577 10490 11950 12966 13345 14432 15014 16081 Size: 519 913 1460 1016 379 1087 582 1067 Cuts at: 16081 17963 19508 1132 Size: 1882 1545 2229 Fragments arranged by size: 3120 2229 1882 1545 1460 1268 1241 1087 1067 1016 1010 913 582 520 519 510 379 160 97 PasI CC'CwG_GG Cuts at: 1348 2120 2512 14743 17368 17675 1348 Size: 772 392 12231 2625 307 4278 Fragments arranged by size: 12231 4278 2625 772 392 307 PciI A'CATG_T Cuts at: 6283 9054 9573 12962 13341 19504 6283 Size: 2771 519 3389 379 6163 7384 Fragments arranged by size: 7384 6163 3389 2771 519 379 PflMI CCAn_nnn'nTGG Cuts at: 1108 6670 10726 13828 14498 17510 1108 Size: 5562 4056 3102 670 3012 4203 Fragments arranged by size: 5562 4203 4056 3102 3012 670 PfoI T'CCnGG_A Cuts at: 4488 5652 5823 6942 7068 7123 7914 8154 8247 Size: 1164 171 1119 126 55 791 240 93 Cuts at: 8247 8586 9831 10351 10695 10800 12336 13662 15969 Size: 339 1245 520 344 105 1536 1326 2307 Cuts at: 15969 16398 17816 4488 Size: 429 1418 7277 Fragments arranged by size: 7277 2307 1536 1418 1326 1245 1164 1119 791 520 429 344 339 240 171 126 105 93 55 PmeI GTTT'AAAC Cuts at: 17909 17909 Size: 20605 PmlI CAC'GTG Cuts at: 6211 6884 6956 6211 Size: 673 72 19860 Fragments arranged by size: 19860 673 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 18674 18706 20187 20219 18674 Size: 32 1481 32 19060 Fragments arranged by size: 19060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 8814 10173 15817 15898 16324 17894 2356 Size: 6458 1359 5644 81 426 1570 5067 Fragments arranged by size: 6458 5644 5067 1570 1359 426 81 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 20605 PsiI TTA'TAA Cuts at: 3063 14907 18175 3063 Size: 11844 3268 5493 Fragments arranged by size: 11844 5493 3268 PspOMI G'GGCC_C Cuts at: 9551 13071 9551 Size: 3520 17085 PstI C_TGCA'G Cuts at: 830 2200 2415 3369 3468 5010 5763 6642 7470 Size: 1370 215 954 99 1542 753 879 828 Cuts at: 7470 7839 8127 8379 8505 9177 9496 10432 10752 Size: 369 288 252 126 672 319 936 320 Cuts at: 10752 10935 13575 14736 16158 17800 17957 830 Size: 183 2640 1161 1422 1642 157 3478 Fragments arranged by size: 3478 2640 1642 1542 1422 1370 1161 954 936 879 828 753 672 369 320 319 288 252 215 183 157 126 99 PvuI CG_AT'CG Cuts at: 2001 10105 2001 Size: 8104 12501 PvuII CAG'CTG Cuts at: 2630 3463 4076 4324 4852 5758 7108 11422 11814 Size: 833 613 248 528 906 1350 4314 392 Cuts at: 11814 12019 12082 12125 12427 14884 15790 17472 2630 Size: 205 63 43 302 2457 906 1682 5763 Fragments arranged by size: 5763 4314 2457 1682 1350 906 906 833 613 528 392 302 248 205 63 43 SacI G_AGCT'C Cuts at: 8447 8595 8663 10395 10918 11838 13116 14529 16312 Size: 148 68 1732 523 920 1278 1413 1783 Cuts at: 16312 16398 8447 Size: 86 12654 Fragments arranged by size: 12654 1783 1732 1413 1278 920 523 148 86 68 SalI G'TCGA_C Cuts at: 1936 5596 11304 17947 1936 Size: 3660 5708 6643 4594 Fragments arranged by size: 6643 5708 4594 3660 SapI GCTCTTCn'nnn_ Cuts at: 3507 3888 6414 6903 10404 10737 12303 13104 13911 Size: 381 2526 489 3501 333 1566 801 807 Cuts at: 13911 13988 15183 17061 3507 Size: 77 1195 1878 7051 Fragments arranged by size: 7051 3501 2526 1878 1566 1195 807 801 489 381 333 77 SbfI CC_TGCA'GG Cuts at: 7839 17957 7839 Size: 10118 10487 ScaI AGT'ACT Cuts at: 5562 6032 8359 9096 18853 20565 5562 Size: 470 2327 737 9757 1712 5602 Fragments arranged by size: 9757 5602 2327 1712 737 470 SexAI A'CCwGG_T Cuts at: 8643 10175 8643 Size: 1532 19073 SfcI C'TryA_G Cuts at: 826 1045 2196 2411 3293 3365 3464 4071 4639 Size: 219 1151 215 882 72 99 607 568 Cuts at: 4639 5006 5759 6638 7466 7835 8058 8095 8123 Size: 367 753 879 828 369 223 37 28 Cuts at: 8123 8361 8375 8423 8501 8735 9173 9492 10241 Size: 238 14 48 78 234 438 319 749 Cuts at: 10241 10253 10428 10748 10885 10931 11455 11756 13571 Size: 12 175 320 137 46 524 301 1815 Cuts at: 13571 14732 16154 17796 17953 19088 19769 19960 826 Size: 1161 1422 1642 157 1135 681 191 1471 Fragments arranged by size: 1815 1642 1471 1422 1161 1151 1135 882 879 828 753 749 681 607 568 524 438 369 367 320 319 301 238 234 223 219 215 191 175 157 137 99 78 72 48 46 37 28 14 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 20605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 20605 SmaI CCC'GGG Cuts at: 1872 2381 17420 17932 1872 Size: 509 15039 512 4545 Fragments arranged by size: 15039 4545 512 509 SmlI C'TyrA_G Cuts at: 820 1017 1943 3183 3904 4247 4408 5792 6150 Size: 197 926 1240 721 343 161 1384 358 Cuts at: 6150 6166 7618 8636 10031 10448 10853 10878 14063 Size: 16 1452 1018 1395 417 405 25 3185 Cuts at: 14063 14644 16031 16872 18709 19610 19872 820 Size: 581 1387 841 1837 901 262 1553 Fragments arranged by size: 3185 1837 1553 1452 1395 1387 1384 1240 1018 926 901 841 721 581 417 405 358 343 262 197 161 25 16 SnaBI TAC'GTA Cuts at: 493 5495 18396 493 Size: 5002 12901 2702 Fragments arranged by size: 12901 5002 2702 SpeI A'CTAG_T Cuts at: 152 2461 12700 152 Size: 2309 10239 8057 Fragments arranged by size: 10239 8057 2309 SphI G_CATG'C Cuts at: 1132 6127 14432 17963 1132 Size: 4995 8305 3531 3774 Fragments arranged by size: 8305 4995 3774 3531 SspI AAT'ATT Cuts at: 5 52 2470 4442 10638 10819 18529 5 Size: 47 2418 1972 6196 181 7710 2081 Fragments arranged by size: 7710 6196 2418 2081 1972 181 47 StuI AGG'CCT Cuts at: 1517 6080 7307 7841 9275 11288 13589 16040 1517 Size: 4563 1227 534 1434 2013 2301 2451 6082 Fragments arranged by size: 6082 4563 2451 2301 2013 1434 1227 534 StyI C'CwwG_G Cuts at: 513 1065 2592 3102 3666 4497 5109 5670 5676 Size: 552 1527 510 564 831 612 561 6 Cuts at: 5676 5854 6724 7167 9019 9210 10078 10505 11796 Size: 178 870 443 1852 191 868 427 1291 Cuts at: 11796 12393 13939 14413 14892 15643 16233 16779 17271 Size: 597 1546 474 479 751 590 546 492 Cuts at: 17271 17517 17806 18044 513 Size: 246 289 238 3074 Fragments arranged by size: 3074 1852 1546 1527 1291 870 868 831 751 612 597 590 564 561 552 546 510 492 479 474 443 427 289 246 238 191 178 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4272 4792 5309 6304 6752 7307 9827 Size: 1255 2692 520 517 995 448 555 2520 Cuts at: 9827 9911 10154 11180 11546 13823 14351 16187 16497 Size: 84 243 1026 366 2277 528 1836 310 Cuts at: 16497 17025 18632 18649 18802 18987 19432 325 Size: 528 1607 17 153 185 445 1498 Fragments arranged by size: 2692 2520 2277 1836 1607 1498 1255 1026 995 555 528 528 520 517 448 445 366 310 243 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3429 3572 4912 Size: 274 80 33 51 1443 1452 143 1340 Cuts at: 4912 5148 5560 6030 7763 8357 9094 9712 11671 Size: 236 412 470 1733 594 737 618 1959 Cuts at: 11671 12693 17474 18851 20563 96 Size: 1022 4781 1377 1712 138 Fragments arranged by size: 4781 1959 1733 1712 1452 1443 1377 1340 1022 737 618 594 470 412 274 236 143 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2933 2965 3200 3232 8559 8591 8934 8966 9032 Size: 32 235 32 5327 32 343 32 66 Cuts at: 9032 9064 9330 9362 10283 10315 10540 10572 12711 Size: 32 266 32 921 32 225 32 2139 Cuts at: 12711 12743 13482 13514 14990 15022 20565 20597 2933 Size: 32 739 32 1476 32 5543 32 2941 Fragments arranged by size: 5543 5327 2941 2139 1476 921 739 343 266 235 225 66 32 32 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5435 13003 15827 15995 16070 16556 17761 1316 Size: 4119 7568 2824 168 75 486 1205 4160 Fragments arranged by size: 7568 4160 4119 2824 1205 486 168 75 XbaI T'CTAG_A Cuts at: 3565 17941 3565 Size: 14376 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 4270 8323 11811 12658 2509 Size: 1761 4053 3488 847 10456 Fragments arranged by size: 10456 4053 3488 1761 847 XhoI C'TCGA_G Cuts at: 1943 5792 16872 1943 Size: 3849 11080 5676 Fragments arranged by size: 11080 5676 3849 XmaI C'CCGG_G Cuts at: 1870 2379 17418 17930 1870 Size: 509 15039 512 4545 Fragments arranged by size: 15039 4545 512 509 XmnI GAAnn'nnTTC Cuts at: 2252 2687 3615 4441 4935 5001 6909 9117 10269 Size: 435 928 826 494 66 1908 2208 1152 Cuts at: 10269 12887 14213 16059 16407 18734 2252 Size: 2618 1326 1846 348 2327 4123 Fragments arranged by size: 4123 2618 2327 2208 1908 1846 1326 1152 928 826 494 435 348 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 18413 276 Size: 53 83 186 936 16879 2468 Fragments arranged by size: 16879 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BbeI BsiWI BstZ17I FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI