(Linear) (Six-base) MAPSORT of: KIEE0600.seq Check: 969 from: 1 to: 15900 KIEE0600.orf With 182 enzymes: SgfI * May 13, 2010 15:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1452 5415 6204 9603 14718 15900 Size: 1452 3963 789 3399 5115 1182 Fragments arranged by size: 5115 3963 3399 1452 1182 789 AccI GT'mk_AC Cuts at: 0 722 3592 4315 6417 6912 8948 9300 9938 Size: 722 2870 723 2102 495 2036 352 638 Cuts at: 9938 10441 11507 15900 Size: 503 1066 4393 Fragments arranged by size: 4393 2870 2102 2036 1066 723 722 638 503 495 352 AclI AA'CG_TT Cuts at: 0 247 15900 Size: 247 15653 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1519 1760 2020 2358 2962 4115 4466 4570 Size: 1519 241 260 338 604 1153 351 104 Cuts at: 4570 4579 5063 5422 5440 6857 6925 7099 8059 Size: 9 484 359 18 1417 68 174 960 Cuts at: 8059 8336 8651 9308 9580 10852 10914 11522 11732 Size: 277 315 657 272 1272 62 608 210 Cuts at: 11732 11999 12085 12700 13486 13918 14114 14702 15761 Size: 267 86 615 786 432 196 588 1059 Cuts at: 15761 15900 Size: 139 Fragments arranged by size: 1519 1417 1272 1153 1059 960 786 657 615 608 604 588 484 432 359 351 338 315 277 272 267 260 241 210 196 174 139 104 86 68 62 18 9 AfeI AGC'GCT Cuts at: 0 3730 15789 15900 Size: 3730 12059 111 Fragments arranged by size: 12059 3730 111 AflII C'TTAA_G Cuts at: 0 4161 12639 15900 Size: 4161 8478 3261 Fragments arranged by size: 8478 4161 3261 AflIII A'CryG_T Cuts at: 0 4278 4948 4950 7049 7568 10957 11336 14920 Size: 4278 670 2 2099 519 3389 379 3584 Cuts at: 14920 15900 Size: 980 Fragments arranged by size: 4278 3584 3389 2099 980 670 519 379 2 AgeI A'CCGG_T Cuts at: 0 1395 15182 15900 Size: 1395 13787 718 Fragments arranged by size: 13787 1395 718 AhdI GACnn_n'nnGTC Cuts at: 0 509 796 5641 11900 12954 15887 15900 Size: 509 287 4845 6259 1054 2933 13 Fragments arranged by size: 6259 4845 2933 1054 509 287 13 AleI CACnn'nnGTG Cuts at: 0 865 10940 12263 15900 Size: 865 10075 1323 3637 Fragments arranged by size: 10075 3637 1323 865 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 385 1385 1849 3275 4550 6299 6786 8223 Size: 385 1000 464 1426 1275 1749 487 1437 Cuts at: 8223 12946 15900 Size: 4723 2954 Fragments arranged by size: 4723 2954 1749 1437 1426 1275 1000 487 464 385 AlwNI CAG_nnn'CTG Cuts at: 0 1479 2043 3037 3093 4407 4665 5106 5352 Size: 1479 564 994 56 1314 258 441 246 Cuts at: 5352 5364 5884 7006 7176 7678 7821 7903 7986 Size: 12 520 1122 170 502 143 82 83 Cuts at: 7986 8976 10177 11117 13896 13971 15900 Size: 990 1201 940 2779 75 1929 Fragments arranged by size: 2779 1929 1479 1314 1201 1122 994 990 940 564 520 502 441 258 246 170 143 83 82 75 56 12 ApaI G_GGCC'C Cuts at: 0 7550 11070 15900 Size: 7550 3520 4830 Fragments arranged by size: 7550 4830 3520 ApaLI G'TGCA_C Cuts at: 0 13804 15900 Size: 13804 2096 ApoI r'AATT_y Cuts at: 0 1088 1156 1944 2050 3209 3630 4012 5271 Size: 1088 68 788 106 1159 421 382 1259 Cuts at: 5271 5455 6790 7293 7304 7382 7612 7934 9254 Size: 184 1335 503 11 78 230 322 1320 Cuts at: 9254 11728 13072 13080 13093 13302 14176 14800 15037 Size: 2474 1344 8 13 209 874 624 237 Cuts at: 15037 15274 15337 15900 Size: 237 63 563 Fragments arranged by size: 2474 1344 1335 1320 1259 1159 1088 874 788 624 563 503 421 382 322 237 237 230 209 184 106 78 68 63 13 11 8 AseI AT'TA_AT Cuts at: 0 9637 15900 Size: 9637 6263 AvaI C'yCGr_G Cuts at: 0 374 1709 3787 5136 5542 11132 11705 14867 Size: 374 1335 2078 1349 406 5590 573 3162 Cuts at: 14867 15413 15900 Size: 546 487 Fragments arranged by size: 5590 3162 2078 1349 1335 573 546 487 406 374 AvrII C'CTAG_G Cuts at: 0 13638 15900 Size: 13638 2262 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 10519 10552 15900 Size: 10519 33 5348 Fragments arranged by size: 10519 5348 33 BamHI G'GATC_C Cuts at: 0 8550 9588 11930 14414 15900 Size: 8550 1038 2342 2484 1486 Fragments arranged by size: 8550 2484 2342 1486 1038 BanI G'GyrC_C Cuts at: 0 2030 2742 3767 10632 15625 15900 Size: 2030 712 1025 6865 4993 275 Fragments arranged by size: 6865 4993 2030 1025 712 275 BanII G_rGCy'C Cuts at: 0 1900 4876 6442 6590 6658 7550 8390 8913 Size: 1900 2976 1566 148 68 892 840 523 Cuts at: 8913 9833 11070 11111 11376 12399 12524 14307 14393 Size: 920 1237 41 265 1023 125 1783 86 Cuts at: 14393 15900 Size: 1507 Fragments arranged by size: 2976 1900 1783 1566 1507 1237 1023 920 892 840 523 265 148 125 86 68 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 863 1525 2480 2999 6611 6742 8090 8495 Size: 863 662 955 519 3612 131 1348 405 Cuts at: 8495 10088 13537 15865 15900 Size: 1593 3449 2328 35 Fragments arranged by size: 3612 3449 2328 1593 1348 955 863 662 519 405 131 35 BbvCI CC'TCA_GC Cuts at: 0 2940 9029 10468 13790 15900 Size: 2940 6089 1439 3322 2110 Fragments arranged by size: 6089 3322 2940 2110 1439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2992 3026 3676 3710 10198 10232 14092 14126 Size: 2992 34 650 34 6488 34 3860 34 Cuts at: 14126 15900 Size: 1774 Fragments arranged by size: 6488 3860 2992 1774 650 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3826 13656 15900 Size: 3826 9830 2244 Fragments arranged by size: 9830 3826 2244 BclI T'GATC_A Cuts at: 0 2714 8657 11169 13295 15900 Size: 2714 5943 2512 2126 2605 Fragments arranged by size: 5943 2714 2605 2512 2126 BfrBI ATG'CAT Cuts at: 0 3262 5523 6763 15900 Size: 3262 2261 1240 9137 Fragments arranged by size: 9137 3262 2261 1240 BglI GCCn_nnn'nGGC Cuts at: 0 4721 7276 15900 Size: 4721 2555 8624 Fragments arranged by size: 8624 4721 2555 BglII A'GATC_T Cuts at: 0 312 542 3564 4466 7026 8852 15900 Size: 312 230 3022 902 2560 1826 7048 Fragments arranged by size: 7048 3022 2560 1826 902 312 230 BlpI GC'TnA_GC Cuts at: 0 3946 4870 5954 6101 9805 10121 10862 11110 Size: 3946 924 1084 147 3704 316 741 248 Cuts at: 11110 14333 14387 14498 15900 Size: 3223 54 111 1402 Fragments arranged by size: 3946 3704 3223 1402 1084 924 741 316 248 147 111 54 Bme1580I G_kGCm'C Cuts at: 0 7550 10637 11070 11139 13808 15900 Size: 7550 3087 433 69 2669 2092 Fragments arranged by size: 7550 3087 2669 2092 433 69 BmgBI CAC'GTC Cuts at: 0 9261 14544 14923 15900 Size: 9261 5283 379 977 Fragments arranged by size: 9261 5283 977 379 BmrI ACTGGGnnnn_n' Cuts at: 0 802 2292 5283 6933 7803 9966 10527 11394 Size: 802 1490 2991 1650 870 2163 561 867 Cuts at: 11394 13259 13876 15900 Size: 1865 617 2024 Fragments arranged by size: 2991 2163 2024 1865 1650 1490 870 867 802 617 561 BmtI G_CTAG'C Cuts at: 0 5147 15900 Size: 5147 10753 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 119 372 1914 5151 7176 9162 10634 15900 Size: 119 253 1542 3237 2025 1986 1472 5266 Fragments arranged by size: 5266 3237 2025 1986 1542 1472 253 119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 687 824 924 1456 1828 2002 2142 2206 Size: 687 137 100 532 372 174 140 64 Cuts at: 2206 2413 2467 3199 3598 4258 4687 4921 4990 Size: 207 54 732 399 660 429 234 69 Cuts at: 4990 5533 5893 5921 6181 7700 7705 7810 8098 Size: 543 360 28 260 1519 5 105 288 Cuts at: 8098 8369 8917 9124 9199 9493 9889 10036 10659 Size: 271 548 207 75 294 396 147 623 Cuts at: 10659 11144 11476 11938 12641 13948 14767 14806 14847 Size: 485 332 462 703 1307 819 39 41 Cuts at: 14847 15426 15473 15795 15869 15900 Size: 579 47 322 74 31 Fragments arranged by size: 1519 1307 819 732 703 687 660 623 579 548 543 532 485 462 429 399 396 372 360 332 322 294 288 271 260 234 207 207 174 147 140 137 105 100 75 74 69 64 54 47 41 39 31 28 5 Bpu10I CC'TnA_GC Cuts at: 0 2940 4916 5297 6252 7658 8785 9029 10468 Size: 2940 1976 381 955 1406 1127 244 1439 Cuts at: 10468 11182 13270 13790 15900 Size: 714 2088 520 2110 Fragments arranged by size: 2940 2110 2088 1976 1439 1406 1127 955 714 520 381 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1163 1884 2263 2424 4130 5634 6652 8011 Size: 1163 721 379 161 1706 1504 1018 1359 Cuts at: 8011 8428 8858 8869 12079 14047 15900 Size: 417 430 11 3210 1968 1853 Fragments arranged by size: 3210 1968 1853 1706 1504 1359 1163 1018 721 430 417 379 161 11 BsaI GGTCTCn'nnnn_ Cuts at: 0 1450 3445 4351 5539 7918 9346 12477 12491 Size: 1450 1995 906 1188 2379 1428 3131 14 Cuts at: 12491 13217 14422 15900 Size: 726 1205 1478 Fragments arranged by size: 3131 2379 1995 1478 1450 1428 1205 1188 906 726 14 BsaAI yAC'GTr Cuts at: 0 3490 4206 4879 4951 15900 Size: 3490 716 673 72 10949 Fragments arranged by size: 10949 3490 716 673 72 BsaBI GATnn'nnATC Cuts at: 0 5769 14293 15071 15900 Size: 5769 8524 778 829 Fragments arranged by size: 8524 5769 829 778 BsaHI Gr'CG_yC Cuts at: 0 4233 15900 Size: 4233 11667 BsaWI w'CCGG_w Cuts at: 0 1395 1580 2021 6794 15182 15900 Size: 1395 185 441 4773 8388 718 Fragments arranged by size: 8388 4773 1395 718 441 185 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 643 673 1903 1933 1975 2005 6154 6184 Size: 643 30 1230 30 42 30 4149 30 Cuts at: 6184 7027 7057 8278 8308 12667 12697 12985 13015 Size: 843 30 1221 30 4359 30 288 30 Cuts at: 13015 14640 14670 15900 Size: 1625 30 1230 Fragments arranged by size: 4359 4149 1625 1230 1230 1221 843 643 288 42 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1725 2113 2116 2203 3073 3139 3196 4255 Size: 1725 388 3 87 870 66 57 1059 Cuts at: 4255 4258 4261 4279 5353 5863 7153 9508 9841 Size: 3 3 18 1074 510 1290 2355 333 Cuts at: 9841 10330 10495 10498 10981 11371 11617 12712 14329 Size: 489 165 3 483 390 246 1095 1617 Cuts at: 14329 14533 14645 14803 15900 Size: 204 112 158 1097 Fragments arranged by size: 2355 1725 1617 1290 1097 1095 1074 1059 870 510 489 483 390 388 333 246 204 165 158 112 87 66 57 18 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1893 2253 3749 3782 3887 4019 4307 4733 Size: 1893 360 1496 33 105 132 288 426 Cuts at: 4733 5261 5364 7496 7542 8135 8704 9038 12671 Size: 528 103 2132 46 593 569 334 3633 Cuts at: 12671 12875 13982 15498 15682 15900 Size: 204 1107 1516 184 218 Fragments arranged by size: 3633 2132 1893 1516 1496 1107 593 569 528 426 360 334 288 218 204 184 132 105 103 46 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 5449 8578 9668 12103 12129 13339 15900 Size: 436 5013 3129 1090 2435 26 1210 2561 Fragments arranged by size: 5013 3129 2561 2435 1210 1090 436 26 BsiEI CG_ry'CG Cuts at: 0 91 1261 7507 8100 15006 15900 Size: 91 1170 6246 593 6906 894 Fragments arranged by size: 6906 6246 1170 894 593 91 BsiHKAI G_wGCw'C Cuts at: 0 3287 4707 5960 6442 6590 6638 6658 7807 Size: 3287 1420 1253 482 148 48 20 1149 Cuts at: 7807 8009 8390 8913 9833 10580 10620 11111 12524 Size: 202 381 523 920 747 40 491 1413 Cuts at: 12524 13190 13587 13808 13978 14127 14307 14393 15306 Size: 666 397 221 170 149 180 86 913 Cuts at: 15306 15900 Size: 594 Fragments arranged by size: 3287 1420 1413 1253 1149 920 913 747 666 594 523 491 482 397 381 221 202 180 170 149 148 86 48 40 20 BsmI GAATG_Cn' Cuts at: 0 4539 7581 15900 Size: 4539 3042 8319 Fragments arranged by size: 8319 4539 3042 BsmBI CGTCTCn'nnnn_ Cuts at: 0 13 12289 15745 15900 Size: 13 12276 3456 155 Fragments arranged by size: 12276 3456 155 13 Bsp1286I G_dGCh'C Cuts at: 0 1900 3287 4707 4876 5960 6442 6590 6638 Size: 1900 1387 1420 169 1084 482 148 48 Cuts at: 6638 6658 7550 7807 8009 8390 8913 9833 10580 Size: 20 892 257 202 381 523 920 747 Cuts at: 10580 10620 10637 11070 11111 11139 11376 12399 12524 Size: 40 17 433 41 28 237 1023 125 Cuts at: 12524 13190 13587 13808 13978 14127 14307 14393 15306 Size: 666 397 221 170 149 180 86 913 Cuts at: 15306 15900 Size: 594 Fragments arranged by size: 1900 1420 1387 1084 1023 920 913 892 747 666 594 523 482 433 397 381 257 237 221 202 180 170 169 149 148 125 86 48 41 40 28 20 17 BspEI T'CCGG_A Cuts at: 0 2021 6794 15900 Size: 2021 4773 9106 Fragments arranged by size: 9106 4773 2021 BspHI T'CATG_A Cuts at: 0 7095 11172 11424 13826 14180 15900 Size: 7095 4077 252 2402 354 1720 Fragments arranged by size: 7095 4077 2402 1720 354 252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 146 1452 1841 2486 4392 5415 6204 9603 Size: 146 1306 389 645 1906 1023 789 3399 Cuts at: 9603 14718 15900 Size: 5115 1182 Fragments arranged by size: 5115 3399 1906 1306 1182 1023 789 645 389 146 BsrBI CCG'CTC Cuts at: 0 33 51 87 8232 8618 11064 13689 14751 Size: 33 18 36 8145 386 2446 2625 1062 Cuts at: 14751 15383 15900 Size: 632 517 Fragments arranged by size: 8145 2625 2446 1062 632 517 386 36 33 18 BsrDI GCAATG_nn' Cuts at: 0 477 1833 2744 3776 5227 6281 9799 12603 Size: 477 1356 911 1032 1451 1054 3518 2804 Cuts at: 12603 15900 Size: 3297 Fragments arranged by size: 3518 3297 2804 1451 1356 1054 1032 911 477 BsrFI r'CCGG_y Cuts at: 0 1260 1395 14021 15182 15713 15900 Size: 1260 135 12626 1161 531 187 Fragments arranged by size: 12626 1260 1161 531 187 135 BsrGI T'GTAC_A Cuts at: 0 1424 1567 2907 3143 5758 9666 15469 15900 Size: 1424 143 1340 236 2615 3908 5803 431 Fragments arranged by size: 5803 3908 2615 1424 1340 431 236 143 BssSI C'ACGA_G Cuts at: 0 2384 5993 7963 15305 15900 Size: 2384 3609 1970 7342 595 Fragments arranged by size: 7342 3609 2384 1970 595 BstAPI GCAn_nnn'nTGC Cuts at: 0 199 414 2847 5349 8125 15900 Size: 199 215 2433 2502 2776 7775 Fragments arranged by size: 7775 2776 2502 2433 215 199 BstBI TT'CG_AA Cuts at: 0 6404 15900 Size: 6404 9496 BstEII G'GTnAC_C Cuts at: 0 1548 6324 14553 15900 Size: 1548 4776 8229 1347 Fragments arranged by size: 8229 4776 1548 1347 BstXI CCAn_nnnn'nTGG Cuts at: 0 2793 3436 3678 15900 Size: 2793 643 242 12222 Fragments arranged by size: 12222 2793 643 242 BstYI r'GATC_y Cuts at: 0 312 542 1467 1475 3564 4466 4671 4740 Size: 312 230 925 8 2089 902 205 69 Cuts at: 4740 5770 6578 7026 7037 8550 8852 8998 9588 Size: 1030 808 448 11 1513 302 146 590 Cuts at: 9588 9884 11637 11930 14414 15900 Size: 296 1753 293 2484 1486 Fragments arranged by size: 2484 2089 1753 1513 1486 1030 925 902 808 590 448 312 302 296 293 230 205 146 69 11 8 Bsu36I CC'TnA_GG Cuts at: 0 902 5336 8942 11291 15900 Size: 902 4434 3606 2349 4609 Fragments arranged by size: 4609 4434 3606 2349 902 BtgI C'CryG_G Cuts at: 0 2492 3104 3849 7205 7507 8500 11549 11595 Size: 2492 612 745 3356 302 993 3049 46 Cuts at: 11595 14228 14695 15900 Size: 2633 467 1205 Fragments arranged by size: 3356 3049 2633 2492 1205 993 745 612 467 302 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 8006 15900 Size: 8006 7894 BtsI GCAGTG_nn' Cuts at: 0 142 1357 2311 3881 4985 5738 6371 7177 Size: 142 1215 954 1570 1104 753 633 806 Cuts at: 7177 9123 15866 15900 Size: 1946 6743 34 Fragments arranged by size: 6743 1946 1570 1215 1104 954 806 753 633 142 34 ClaI AT'CG_AT Cuts at: 0 5054 14936 15900 Size: 5054 9882 964 Fragments arranged by size: 9882 5054 964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 4797 4832 4865 4900 9357 9392 14245 14280 Size: 4797 35 33 35 4457 35 4853 35 Cuts at: 14280 15104 15139 15900 Size: 824 35 761 Fragments arranged by size: 4853 4797 4457 824 761 35 35 35 35 35 33 DraI TTT'AAA Cuts at: 0 9564 10232 13130 15900 Size: 9564 668 2898 2770 Fragments arranged by size: 9564 2898 2770 668 DraIII CAC_nnn'GTG Cuts at: 0 257 15900 Size: 257 15643 DrdI GACnn_nn'nnGTC Cuts at: 0 12505 14821 15900 Size: 12505 2316 1079 Fragments arranged by size: 12505 2316 1079 EaeI y'GGCC_r Cuts at: 0 324 1094 1161 1258 1724 2795 3134 4475 Size: 324 770 67 97 466 1071 339 1341 Cuts at: 4475 5282 5319 7504 9114 9627 10097 10424 15900 Size: 807 37 2185 1610 513 470 327 5476 Fragments arranged by size: 5476 2185 1610 1341 1071 807 770 513 470 466 339 327 324 97 67 37 EagI C'GGCC_G Cuts at: 0 1258 7504 15900 Size: 1258 6246 8396 Fragments arranged by size: 8396 6246 1258 EarI CTCTTCn'nnn_ Cuts at: 0 11 1202 1502 1784 1883 3050 4134 4256 Size: 11 1191 300 282 99 1167 1084 122 Cuts at: 4256 4409 4847 4898 4937 6901 7916 8255 8348 Size: 153 438 51 39 1964 1015 339 93 Cuts at: 8348 8399 8732 9311 9599 9686 10298 10933 11099 Size: 51 333 579 288 87 612 635 166 Cuts at: 11099 11885 11906 11983 12104 12458 12887 13043 13178 Size: 786 21 77 121 354 429 156 135 Cuts at: 13178 15056 15900 Size: 1878 844 Fragments arranged by size: 1964 1878 1191 1167 1084 1015 844 786 635 612 579 438 429 354 339 333 300 288 282 166 156 153 135 122 121 99 93 87 77 51 51 39 21 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 4977 15900 Size: 4977 10923 EcoICRI GAG'CTC Cuts at: 0 6440 6588 6656 8388 8911 9831 11109 12522 Size: 6440 148 68 1732 523 920 1278 1413 Cuts at: 12522 14305 14391 15900 Size: 1783 86 1509 Fragments arranged by size: 6440 1783 1732 1509 1413 1278 920 523 148 86 68 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 687 824 924 1456 1519 1760 1828 2002 Size: 687 137 100 532 63 241 68 174 Cuts at: 2002 2020 2142 2206 2358 2413 2467 2962 3199 Size: 18 122 64 152 55 54 495 237 Cuts at: 3199 3598 4115 4258 4466 4570 4579 4687 4921 Size: 399 517 143 208 104 9 108 234 Cuts at: 4921 4990 5063 5422 5440 5533 5893 5921 6181 Size: 69 73 359 18 93 360 28 260 Cuts at: 6181 6857 6925 7099 7700 7705 7810 8059 8098 Size: 676 68 174 601 5 105 249 39 Cuts at: 8098 8336 8369 8651 8917 9124 9199 9308 9493 Size: 238 33 282 266 207 75 109 185 Cuts at: 9493 9580 9889 10036 10659 10852 10914 11144 11476 Size: 87 309 147 623 193 62 230 332 Cuts at: 11476 11522 11732 11938 11999 12085 12641 12700 13486 Size: 46 210 206 61 86 556 59 786 Cuts at: 13486 13918 13948 14114 14702 14767 14806 14847 15426 Size: 432 30 166 588 65 39 41 579 Cuts at: 15426 15473 15761 15795 15869 15900 Size: 47 288 34 74 31 Fragments arranged by size: 786 687 676 623 601 588 579 556 532 517 495 432 399 360 359 332 309 288 282 266 260 249 241 238 237 234 230 210 208 207 206 193 185 174 174 166 152 147 143 137 122 109 108 105 104 100 93 87 86 75 74 73 69 68 68 65 64 63 62 61 59 55 54 47 46 41 39 39 34 33 31 30 28 18 18 9 5 EcoNI CCTnn'n_nnAGG Cuts at: 0 2482 3652 4543 8072 10843 11294 15349 15876 Size: 2482 1170 891 3529 2771 451 4055 527 Cuts at: 15876 15900 Size: 24 Fragments arranged by size: 4055 3529 2771 2482 1170 891 527 451 24 EcoO109I rG'GnC_Cy Cuts at: 0 351 6809 7546 7547 7675 8168 8931 9504 Size: 351 6458 737 1 128 493 763 573 Cuts at: 9504 10392 13053 13812 13893 14145 14319 15889 15900 Size: 888 2661 759 81 252 174 1570 11 Fragments arranged by size: 6458 2661 1570 888 763 759 737 573 493 351 252 174 128 81 11 1 EcoRI G'AATT_C Cuts at: 0 3209 3630 6790 7293 13072 13080 14176 15900 Size: 3209 421 3160 503 5779 8 1096 1724 Fragments arranged by size: 5779 3209 3160 1724 1096 503 421 8 EcoRV GAT'ATC Cuts at: 0 13475 15900 Size: 13475 2425 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 6318 15746 15900 Size: 6318 9428 154 Fragments arranged by size: 9428 6318 154 HaeII r_GCGC'y Cuts at: 0 3732 4113 4936 8616 11595 15791 15900 Size: 3732 381 823 3680 2979 4196 109 Fragments arranged by size: 4196 3732 3680 2979 823 381 109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 495 527 782 814 820 835 852 867 Size: 495 32 255 32 6 15 17 15 Cuts at: 867 902 934 2380 2412 3681 3713 5043 5075 Size: 35 32 1446 32 1269 32 1330 32 Cuts at: 5075 5627 5659 6751 6783 7201 7233 7716 7748 Size: 552 32 1092 32 418 32 483 32 Cuts at: 7748 7984 8016 8419 8451 9132 9164 9687 9719 Size: 236 32 403 32 681 32 523 32 Cuts at: 9719 9986 10018 10126 10158 10845 10877 10986 11018 Size: 267 32 108 32 687 32 109 32 Cuts at: 11018 11510 11542 11886 11918 12888 12920 12940 12972 Size: 492 32 344 32 970 32 20 32 Cuts at: 12972 13214 13246 14051 14083 14200 14225 14232 14257 Size: 242 32 805 32 117 25 7 25 Cuts at: 14257 14907 14939 15343 15375 15742 15774 15873 15900 Size: 650 32 404 32 367 32 99 27 Fragments arranged by size: 1446 1330 1269 1092 970 805 687 681 650 552 523 495 492 483 418 404 403 367 344 267 255 242 236 117 109 108 99 35 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 25 25 20 17 15 15 7 6 HincII GTy'rAC Cuts at: 0 264 503 2174 2856 3593 4886 9301 12585 Size: 264 239 1671 682 737 1293 4415 3284 Cuts at: 12585 12722 13758 14214 15900 Size: 137 1036 456 1686 Fragments arranged by size: 4415 3284 1686 1671 1293 1036 737 682 456 264 239 137 HindIII A'AGCT_T Cuts at: 0 2929 3068 4903 5577 6451 8802 9758 10159 Size: 2929 139 1835 674 874 2351 956 401 Cuts at: 10159 12250 13409 15900 Size: 2091 1159 2491 Fragments arranged by size: 2929 2491 2351 2091 1835 1159 956 874 674 401 139 HpaI GTT'AAC Cuts at: 0 2856 14214 15900 Size: 2856 11358 1686 Fragments arranged by size: 11358 2856 1686 MfeI C'AATT_G Cuts at: 0 3307 4528 6128 6957 7915 9151 15900 Size: 3307 1221 1600 829 958 1236 6749 Fragments arranged by size: 6749 3307 1600 1236 1221 958 829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 22 40 326 5471 6502 6597 6764 7249 Size: 22 18 286 5145 1031 95 167 485 Cuts at: 7249 7265 7836 8156 10571 11426 13682 14060 14827 Size: 16 571 320 2415 855 2256 378 767 Cuts at: 14827 15553 15794 15900 Size: 726 241 106 Fragments arranged by size: 5145 2415 2256 1031 855 767 726 571 485 378 320 286 241 167 106 95 22 18 16 MscI TGG'CCA Cuts at: 0 326 1096 1726 2797 3136 4477 5284 5321 Size: 326 770 630 1071 339 1341 807 37 Cuts at: 5321 9116 9629 10099 10426 15900 Size: 3795 513 470 327 5474 Fragments arranged by size: 5474 3795 1341 1071 807 770 630 513 470 339 327 326 37 MslI CAynn'nnrTG Cuts at: 0 296 865 1193 1231 1880 2791 3434 5188 Size: 296 569 328 38 649 911 643 1754 Cuts at: 5188 6038 6262 10940 12263 15235 15900 Size: 850 224 4678 1323 2972 665 Fragments arranged by size: 4678 2972 1754 1323 911 850 665 649 643 569 328 296 224 38 MspA1I CmG'CkG Cuts at: 0 625 1458 2071 2319 2697 2847 3753 5103 Size: 625 833 613 248 378 150 906 1350 Cuts at: 5103 6399 9417 9809 9909 10014 10077 10120 10422 Size: 1296 3018 392 100 105 63 43 302 Cuts at: 10422 12879 13785 14445 14621 14720 15467 15900 Size: 2457 906 660 176 99 747 433 Fragments arranged by size: 3018 2457 1350 1296 906 906 833 747 660 625 613 433 392 378 302 248 176 150 105 100 99 63 43 NaeI GCC'GGC Cuts at: 0 1262 14023 15715 15900 Size: 1262 12761 1692 185 Fragments arranged by size: 12761 1692 1262 185 NcoI C'CATG_G Cuts at: 0 2492 3104 3849 7205 8500 14228 15900 Size: 2492 612 745 3356 1295 5728 1672 Fragments arranged by size: 5728 3356 2492 1672 1295 745 612 NdeI CA'TA_TG Cuts at: 0 2115 9305 12860 15900 Size: 2115 7190 3555 3040 Fragments arranged by size: 7190 3555 3040 2115 NgoMIV G'CCGG_C Cuts at: 0 1260 14021 15713 15900 Size: 1260 12761 1692 187 Fragments arranged by size: 12761 1692 1260 187 NheI G'CTAG_C Cuts at: 0 5143 15900 Size: 5143 10757 NsiI A_TGCA'T Cuts at: 0 3264 5525 6765 15900 Size: 3264 2261 1240 9135 Fragments arranged by size: 9135 3264 2261 1240 NspI r_CATG'y Cuts at: 0 2344 2854 4122 4282 5523 6043 7053 7572 Size: 2344 510 1268 160 1241 520 1010 519 Cuts at: 7572 8485 9945 10961 11340 12427 13009 14076 15900 Size: 913 1460 1016 379 1087 582 1067 1824 Fragments arranged by size: 2344 1824 1460 1268 1241 1087 1067 1016 1010 913 582 520 519 510 379 160 PasI CC'CwG_GG Cuts at: 0 115 507 12738 15363 15670 15900 Size: 115 392 12231 2625 307 230 Fragments arranged by size: 12231 2625 392 307 230 115 PciI A'CATG_T Cuts at: 0 4278 7049 7568 10957 11336 15900 Size: 4278 2771 519 3389 379 4564 Fragments arranged by size: 4564 4278 3389 2771 519 379 PflMI CCAn_nnn'nTGG Cuts at: 0 4665 8721 11823 12493 15505 15900 Size: 4665 4056 3102 670 3012 395 Fragments arranged by size: 4665 4056 3102 3012 670 395 PfoI T'CCnGG_A Cuts at: 0 2483 3647 3818 4937 5063 5118 5909 6149 Size: 2483 1164 171 1119 126 55 791 240 Cuts at: 6149 6242 6581 7826 8346 8690 8795 10331 11657 Size: 93 339 1245 520 344 105 1536 1326 Cuts at: 11657 13964 14393 15811 15900 Size: 2307 429 1418 89 Fragments arranged by size: 2483 2307 1536 1418 1326 1245 1164 1119 791 520 429 344 339 240 171 126 105 93 89 55 PmlI CAC'GTG Cuts at: 0 4206 4879 4951 15900 Size: 4206 673 72 10949 Fragments arranged by size: 10949 4206 673 72 PpuMI rG'GwC_Cy Cuts at: 0 351 6809 8168 13812 13893 14319 15889 15900 Size: 351 6458 1359 5644 81 426 1570 11 Fragments arranged by size: 6458 5644 1570 1359 426 351 81 11 PsiI TTA'TAA Cuts at: 0 1058 12902 15900 Size: 1058 11844 2998 Fragments arranged by size: 11844 2998 1058 PspOMI G'GGCC_C Cuts at: 0 7546 11066 15900 Size: 7546 3520 4834 Fragments arranged by size: 7546 4834 3520 PstI C_TGCA'G Cuts at: 0 195 410 1364 1463 3005 3758 4637 5465 Size: 195 215 954 99 1542 753 879 828 Cuts at: 5465 5834 6122 6374 6500 7172 7491 8427 8747 Size: 369 288 252 126 672 319 936 320 Cuts at: 8747 8930 11570 12731 14153 15795 15900 Size: 183 2640 1161 1422 1642 105 Fragments arranged by size: 2640 1642 1542 1422 1161 954 936 879 828 753 672 369 320 319 288 252 215 195 183 126 105 99 PvuI CG_AT'CG Cuts at: 0 8100 15900 Size: 8100 7800 PvuII CAG'CTG Cuts at: 0 625 1458 2071 2319 2847 3753 5103 9417 Size: 625 833 613 248 528 906 1350 4314 Cuts at: 9417 9809 10014 10077 10120 10422 12879 13785 15467 Size: 392 205 63 43 302 2457 906 1682 Cuts at: 15467 15900 Size: 433 Fragments arranged by size: 4314 2457 1682 1350 906 906 833 625 613 528 433 392 302 248 205 63 43 SacI G_AGCT'C Cuts at: 0 6442 6590 6658 8390 8913 9833 11111 12524 Size: 6442 148 68 1732 523 920 1278 1413 Cuts at: 12524 14307 14393 15900 Size: 1783 86 1507 Fragments arranged by size: 6442 1783 1732 1507 1413 1278 920 523 148 86 68 SalI G'TCGA_C Cuts at: 0 3591 9299 15900 Size: 3591 5708 6601 Fragments arranged by size: 6601 5708 3591 SapI GCTCTTCn'nnn_ Cuts at: 0 1502 1883 4409 4898 8399 8732 10298 11099 Size: 1502 381 2526 489 3501 333 1566 801 Cuts at: 11099 11906 11983 13178 15056 15900 Size: 807 77 1195 1878 844 Fragments arranged by size: 3501 2526 1878 1566 1502 1195 844 807 801 489 381 333 77 SbfI CC_TGCA'GG Cuts at: 0 5834 15900 Size: 5834 10066 ScaI AGT'ACT Cuts at: 0 3557 4027 6354 7091 15900 Size: 3557 470 2327 737 8809 Fragments arranged by size: 8809 3557 2327 737 470 SexAI A'CCwGG_T Cuts at: 0 6638 8170 15900 Size: 6638 1532 7730 Fragments arranged by size: 7730 6638 1532 SfcI C'TryA_G Cuts at: 0 191 406 1288 1360 1459 2066 2634 3001 Size: 191 215 882 72 99 607 568 367 Cuts at: 3001 3754 4633 5461 5830 6053 6090 6118 6356 Size: 753 879 828 369 223 37 28 238 Cuts at: 6356 6370 6418 6496 6730 7168 7487 8236 8248 Size: 14 48 78 234 438 319 749 12 Cuts at: 8248 8423 8743 8880 8926 9450 9751 11566 12727 Size: 175 320 137 46 524 301 1815 1161 Cuts at: 12727 14149 15791 15900 Size: 1422 1642 109 Fragments arranged by size: 1815 1642 1422 1161 882 879 828 753 749 607 568 524 438 369 367 320 319 301 238 234 223 215 191 175 137 109 99 78 72 48 46 37 28 14 12 SmaI CCC'GGG Cuts at: 0 376 15415 15900 Size: 376 15039 485 Fragments arranged by size: 15039 485 376 SmlI C'TyrA_G Cuts at: 0 1178 1899 2242 2403 3787 4145 4161 5613 Size: 1178 721 343 161 1384 358 16 1452 Cuts at: 5613 6631 8026 8443 8848 8873 12058 12639 14026 Size: 1018 1395 417 405 25 3185 581 1387 Cuts at: 14026 14867 15900 Size: 841 1033 Fragments arranged by size: 3185 1452 1395 1387 1384 1178 1033 1018 841 721 581 417 405 358 343 161 25 16 SnaBI TAC'GTA Cuts at: 0 3490 15900 Size: 3490 12410 SpeI A'CTAG_T Cuts at: 0 456 10695 15900 Size: 456 10239 5205 Fragments arranged by size: 10239 5205 456 SphI G_CATG'C Cuts at: 0 4122 12427 15900 Size: 4122 8305 3473 Fragments arranged by size: 8305 4122 3473 SspI AAT'ATT Cuts at: 0 465 2437 8633 8814 15900 Size: 465 1972 6196 181 7086 Fragments arranged by size: 7086 6196 1972 465 181 StuI AGG'CCT Cuts at: 0 4075 5302 5836 7270 9283 11584 14035 15900 Size: 4075 1227 534 1434 2013 2301 2451 1865 Fragments arranged by size: 4075 2451 2301 2013 1865 1434 1227 534 StyI C'CwwG_G Cuts at: 0 587 1097 1661 2492 3104 3665 3671 3849 Size: 587 510 564 831 612 561 6 178 Cuts at: 3849 4719 5162 7014 7205 8073 8500 9791 10388 Size: 870 443 1852 191 868 427 1291 597 Cuts at: 10388 11934 12408 12887 13638 14228 14774 15266 15512 Size: 1546 474 479 751 590 546 492 246 Cuts at: 15512 15801 15900 Size: 289 99 Fragments arranged by size: 1852 1546 1291 870 868 831 751 612 597 590 587 564 561 546 510 492 479 474 443 427 289 246 191 178 99 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2267 2787 3304 4299 4747 5302 7822 7906 Size: 2267 520 517 995 448 555 2520 84 Cuts at: 7906 8149 9175 9541 11818 12346 14182 14492 15020 Size: 243 1026 366 2277 528 1836 310 528 Cuts at: 15020 15900 Size: 880 Fragments arranged by size: 2520 2277 2267 1836 1026 995 880 555 528 528 520 517 448 366 310 243 84 TatI w'GTAC_w Cuts at: 0 1424 1567 2907 3143 3555 4025 5758 6352 Size: 1424 143 1340 236 412 470 1733 594 Cuts at: 6352 7089 7707 9666 10688 15469 15900 Size: 737 618 1959 1022 4781 431 Fragments arranged by size: 4781 1959 1733 1424 1340 1022 737 618 594 470 431 412 236 143 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 928 960 1195 1227 6554 6586 6929 6961 Size: 928 32 235 32 5327 32 343 32 Cuts at: 6961 7027 7059 7325 7357 8278 8310 8535 8567 Size: 66 32 266 32 921 32 225 32 Cuts at: 8567 10706 10738 11477 11509 12985 13017 15900 Size: 2139 32 739 32 1476 32 2883 Fragments arranged by size: 5327 2883 2139 1476 928 921 739 343 266 235 225 66 32 32 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3430 10998 13822 13990 14065 14551 15756 15900 Size: 3430 7568 2824 168 75 486 1205 144 Fragments arranged by size: 7568 3430 2824 1205 486 168 144 75 XbaI T'CTAG_A Cuts at: 0 1560 15900 Size: 1560 14340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 504 2265 6318 9806 10653 15900 Size: 504 1761 4053 3488 847 5247 Fragments arranged by size: 5247 4053 3488 1761 847 504 XhoI C'TCGA_G Cuts at: 0 3787 14867 15900 Size: 3787 11080 1033 Fragments arranged by size: 11080 3787 1033 XmaI C'CCGG_G Cuts at: 0 374 15413 15900 Size: 374 15039 487 Fragments arranged by size: 15039 487 374 XmnI GAAnn'nnTTC Cuts at: 0 247 682 1610 2436 2930 2996 4904 7112 Size: 247 435 928 826 494 66 1908 2208 Cuts at: 7112 8264 10882 12208 14054 14402 15900 Size: 1152 2618 1326 1846 348 1498 Fragments arranged by size: 2618 2208 1908 1846 1498 1326 1152 928 826 494 435 348 247 66 Enzymes that do cut: AarI AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AloI AscI AsiSI BbeI BsiWI BssHII BstZ17I FseI FspI FspAI KasI KpnI MluI NarI NotI NruI PacI PmeI PpiI PshAI PspXI PsrI RsrII SacII SanDI SfiI SfoI SgfI SgrAI SrfI SwaI ZraI