(Circular) (Six-base) MAPSORT of: pFN21AB6207.seq Check: 3655 from: 1 to: 6700 pFN21AB6207 6700 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4510 278 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4043 1937 Size: 2106 4594 Acc65I G'GTAC_C Cuts at: 4021 4021 Size: 6700 AclI AA'CG_TT Cuts at: 1563 4827 5200 1563 Size: 3264 373 3063 Fragments arranged by size: 3264 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 2528 3112 3448 3503 3671 3700 3868 Size: 821 75 584 336 55 168 29 168 Cuts at: 3868 4763 6147 6415 1632 Size: 895 1384 268 1917 Fragments arranged by size: 1917 1384 895 821 584 336 268 168 168 75 55 29 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6503 AflIII A'CryG_T Cuts at: 1203 3826 5599 6576 1203 Size: 2623 1773 977 1327 Fragments arranged by size: 2623 1773 1327 977 AgeI A'CCGG_T Cuts at: 5531 5531 Size: 6700 AhdI GACnn_n'nnGTC Cuts at: 3621 5429 3621 Size: 1808 4892 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6700 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3196 1184 Size: 2012 4688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2964 2996 2964 Size: 32 6668 AlwNI CAG_nnn'CTG Cuts at: 4443 6015 4443 Size: 1572 5128 ApaI G_GGCC'C Cuts at: 3990 3990 Size: 6700 ApaLI G'TGCA_C Cuts at: 3572 4757 5913 3572 Size: 1185 1156 4359 Fragments arranged by size: 4359 1185 1156 ApoI r'AATT_y Cuts at: 1493 2994 3037 4009 4235 4387 6496 1493 Size: 1501 43 972 226 152 2109 1697 Fragments arranged by size: 2109 1697 1501 972 226 152 43 AseI AT'TA_AT Cuts at: 160 1032 5254 160 Size: 872 4222 1606 Fragments arranged by size: 4222 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 AvaI C'yCGr_G Cuts at: 1870 1943 2376 3071 4025 1870 Size: 73 433 695 954 4545 Fragments arranged by size: 4545 954 695 433 73 BamHI G'GATC_C Cuts at: 4030 4397 4030 Size: 367 6333 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2341 4021 5476 618 Size: 325 217 627 554 1680 1455 1842 Fragments arranged by size: 1842 1680 1455 627 554 325 217 BanII G_rGCy'C Cuts at: 1391 3726 3990 4019 1391 Size: 2335 264 29 4072 Fragments arranged by size: 4072 2335 264 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3167 3491 3801 928 Size: 971 1268 324 310 3827 Fragments arranged by size: 3827 1268 971 324 310 BbvCI CC'TCA_GC Cuts at: 2913 2913 Size: 6700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4891 4925 1903 Size: 34 2954 34 3678 Fragments arranged by size: 3678 2954 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3576 4592 5802 3576 Size: 1016 1210 4474 Fragments arranged by size: 4474 1210 1016 BclI T'GATC_A Cuts at: 1548 2734 1548 Size: 1186 5514 BfrBI ATG'CAT Cuts at: 3104 3776 3104 Size: 672 6028 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2729 5311 136 Size: 107 122 71 631 605 1057 2582 1525 Fragments arranged by size: 2582 1525 1057 631 605 122 107 71 BglII A'GATC_T Cuts at: 6695 6695 Size: 6700 BlpI GC'TnA_GC Cuts at: 4117 4117 Size: 6700 Bme1580I G_kGCm'C Cuts at: 1790 2346 3058 3072 3576 3990 4761 5917 1790 Size: 556 712 14 504 414 771 1156 2573 Fragments arranged by size: 2573 1156 771 712 556 504 414 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6700 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 2489 4431 Size: 416 53 83 399 24 728 344 1942 Cuts at: 4431 5389 442 Size: 958 1753 Fragments arranged by size: 1942 1753 958 728 416 399 344 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6700 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2945 2225 Size: 720 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2278 2381 2723 2940 3024 3444 3696 Size: 871 168 103 342 217 84 420 252 Cuts at: 3696 5360 1239 Size: 1664 2579 Fragments arranged by size: 2579 1664 871 420 342 252 217 168 103 84 Bpu10I CC'TnA_GC Cuts at: 2913 2913 Size: 6700 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2969 3652 4825 5690 5988 2969 Size: 683 1173 865 298 3681 Fragments arranged by size: 3681 1173 865 683 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2418 3844 3929 5363 882 Size: 617 919 1426 85 1434 2219 Fragments arranged by size: 2219 1434 1426 919 617 85 BsaAI yAC'GTr Cuts at: 493 1204 2528 3409 4491 493 Size: 711 1324 881 1082 2702 Fragments arranged by size: 2702 1324 1082 881 711 BsaBI GATnn'nnATC Cuts at: 6694 6694 Size: 6700 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2805 4507 4889 5524 Size: 53 83 186 936 1272 1702 382 635 Cuts at: 5524 275 Size: 1451 Fragments arranged by size: 1702 1451 1272 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3254 3549 5133 5531 5805 5952 6363 3254 Size: 295 1584 398 274 147 411 3591 Fragments arranged by size: 3591 1584 411 398 295 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3587 3617 1178 Size: 30 165 30 2184 30 4261 Fragments arranged by size: 4261 2184 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2092 2692 2793 3886 1186 Size: 906 600 101 1093 4000 Fragments arranged by size: 4000 1093 906 600 101 BseYI C'CCAG_C Cuts at: 2163 2167 5903 6399 2163 Size: 4 3736 496 2464 Fragments arranged by size: 3736 2464 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2131 2508 2844 1163 Size: 573 395 377 336 5019 Fragments arranged by size: 5019 573 395 377 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4150 4911 5939 1540 Size: 275 186 2149 761 1028 2301 Fragments arranged by size: 2301 2149 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3560 3576 4761 4846 5917 3560 Size: 16 1185 85 1071 4343 Fragments arranged by size: 4343 1185 1071 85 16 BsmI GAATG_Cn' Cuts at: 3446 3530 3791 4211 4304 3446 Size: 84 261 420 93 5842 Fragments arranged by size: 5842 420 261 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 3058 3072 3560 3576 3726 3990 Size: 399 556 712 14 488 16 150 264 Cuts at: 3990 4019 4761 4846 5917 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 556 488 399 264 150 85 29 16 14 BspEI T'CCGG_A Cuts at: 3549 6363 3549 Size: 2814 3886 BspHI T'CATG_A Cuts at: 4589 6319 4589 Size: 1730 4970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4055 844 Size: 3211 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4587 1394 Size: 429 2764 3507 Fragments arranged by size: 3507 2764 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2057 5195 5369 65 Size: 1183 809 3138 174 1396 Fragments arranged by size: 3138 1396 1183 809 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2385 5344 5531 1700 Size: 110 575 2959 187 2869 Fragments arranged by size: 2959 2869 575 187 110 BsrGI T'GTAC_A Cuts at: 96 3823 3907 96 Size: 3727 84 2889 Fragments arranged by size: 3727 2889 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6700 BssSI C'ACGA_G Cuts at: 3298 4760 5772 3298 Size: 1462 1012 4226 Fragments arranged by size: 4226 1462 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2341 794 Size: 1547 5153 BstBI TT'CG_AA Cuts at: 5504 5504 Size: 6700 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6700 BstXI CCAn_nnnn'nTGG Cuts at: 2652 3704 2652 Size: 1052 5648 BstYI r'GATC_y Cuts at: 1972 2272 2880 4030 4397 4782 4799 6251 6337 Size: 300 608 1150 367 385 17 1452 86 Cuts at: 6337 6349 6695 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1150 608 385 367 346 300 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2327 3398 3734 2327 Size: 1071 336 5293 Fragments arranged by size: 5293 1071 336 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2495 513 Size: 552 939 30 461 4718 Fragments arranged by size: 4718 939 552 461 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5961 Fragments arranged by size: 5961 683 56 BtsI GCAGTG_nn' Cuts at: 2792 4216 5010 5030 2792 Size: 1424 794 20 4462 Fragments arranged by size: 4462 1424 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6700 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2206 2241 3517 3552 529 Size: 35 1642 35 1276 35 3677 Fragments arranged by size: 3677 1642 1276 35 35 35 DraI TTT'AAA Cuts at: 4004 4004 Size: 6700 DraIII CAC_nnn'GTG Cuts at: 3842 3842 Size: 6700 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2804 5707 809 Size: 507 1488 2903 1802 Fragments arranged by size: 2903 1802 1488 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2387 2502 Size: 54 1425 50 275 18 201 356 115 Cuts at: 2502 4147 5036 6665 8 Size: 1645 889 1629 43 Fragments arranged by size: 1645 1629 1425 889 356 275 201 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4147 1537 Size: 275 2335 4090 Fragments arranged by size: 4090 2335 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 2349 3481 3647 3925 4630 1353 Size: 894 102 1132 166 278 705 3423 Fragments arranged by size: 3423 1132 894 705 278 166 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5287 5661 5807 387 Size: 1064 138 3698 374 146 1280 Fragments arranged by size: 3698 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2278 2381 2453 2528 2723 2940 Size: 393 478 168 103 72 75 195 217 Cuts at: 2940 3024 3112 3444 3448 3503 3671 3696 3700 Size: 84 88 332 4 55 168 25 4 Cuts at: 3700 3868 4763 5360 6147 6415 1239 Size: 168 895 597 787 268 1524 Fragments arranged by size: 1524 895 787 597 478 393 332 268 217 195 168 168 168 103 88 84 75 72 55 25 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 3298 3298 Size: 6700 EcoO109I rG'GnC_Cy Cuts at: 2115 3987 2115 Size: 1872 4828 EcoRI G'AATT_C Cuts at: 2994 4009 4387 2994 Size: 1015 378 5307 Fragments arranged by size: 5307 1015 378 FspI TGC'GCA Cuts at: 5206 5206 Size: 6700 HaeII r_GCGC'y Cuts at: 1678 5847 1678 Size: 4169 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2870 2902 3544 3547 3576 Size: 32 135 32 1298 32 642 3 29 Cuts at: 3576 3579 3607 3639 3713 3745 3841 3873 5341 Size: 3 28 32 74 32 96 32 1468 Cuts at: 5341 5373 5415 5447 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1468 1298 642 135 96 74 42 32 32 32 32 32 32 32 32 29 28 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 2840 4044 4290 669 Size: 988 281 902 1204 246 3079 Fragments arranged by size: 3079 1204 988 902 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6700 HpaI GTT'AAC Cuts at: 4290 4290 Size: 6700 KpnI G_GTAC'C Cuts at: 4025 4025 Size: 6700 MfeI C'AATT_G Cuts at: 4299 4299 Size: 6700 MluI A'CGCG_T Cuts at: 6576 6576 Size: 6700 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5814 5998 1305 Size: 4509 184 2007 Fragments arranged by size: 4509 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2504 10 Size: 54 1425 343 672 4206 Fragments arranged by size: 4206 1425 672 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2894 3271 3523 4658 5017 5176 Size: 648 1140 588 377 252 1135 359 159 Cuts at: 5176 518 Size: 2042 Fragments arranged by size: 2042 1140 1135 648 588 377 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2167 2338 4116 4793 5941 6186 1606 Size: 561 171 1778 677 1148 245 2120 Fragments arranged by size: 2120 1778 1148 677 561 245 171 NcoI C'CATG_G Cuts at: 513 1065 2004 2495 513 Size: 552 939 491 4718 Fragments arranged by size: 4718 939 552 491 NdeI CA'TA_TG Cuts at: 387 3520 387 Size: 3133 3567 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6700 NotI GC'GGCC_GC Cuts at: 4147 4147 Size: 6700 NsiI A_TGCA'T Cuts at: 3106 3778 3106 Size: 672 6028 NspI r_CATG'y Cuts at: 1132 1229 2305 3108 3664 3746 3780 3830 4058 Size: 97 1076 803 556 82 34 50 228 Cuts at: 4058 5603 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1076 803 556 228 97 82 50 34 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6700 PciI A'CATG_T Cuts at: 3826 5599 3826 Size: 1773 4927 PflMI CCAn_nnn'nTGG Cuts at: 1108 3983 1108 Size: 2875 3825 PfoI T'CCnGG_A Cuts at: 2046 2397 2046 Size: 351 6349 PmeI GTTT'AAAC Cuts at: 4004 4004 Size: 6700 PmlI CAC'GTG Cuts at: 2528 3409 2528 Size: 881 5819 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2740 4769 4801 6282 6314 2708 Size: 32 2029 32 1481 32 3094 Fragments arranged by size: 3094 2029 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2115 2115 Size: 6700 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6700 PsiI TTA'TAA Cuts at: 4270 4270 Size: 6700 PspOMI G'GGCC_C Cuts at: 3986 3986 Size: 6700 PstI C_TGCA'G Cuts at: 830 3175 4052 830 Size: 2345 877 3478 Fragments arranged by size: 3478 2345 877 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6700 PvuII CAG'CTG Cuts at: 2167 2338 2167 Size: 171 6529 SalI G'TCGA_C Cuts at: 1936 4042 1936 Size: 2106 4594 SbfI CC_TGCA'GG Cuts at: 4052 4052 Size: 6700 ScaI AGT'ACT Cuts at: 4948 6660 4948 Size: 1712 4988 SfcI C'TryA_G Cuts at: 826 1045 2710 3171 4048 5183 5864 6055 826 Size: 219 1665 461 877 1135 681 191 1471 Fragments arranged by size: 1665 1471 1135 877 681 461 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6700 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6700 SmaI CCC'GGG Cuts at: 1872 4027 1872 Size: 2155 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2376 2948 3631 4804 5705 5967 Size: 197 926 433 572 683 1173 901 262 Cuts at: 5967 820 Size: 1553 Fragments arranged by size: 1553 1173 926 901 683 572 433 262 197 SnaBI TAC'GTA Cuts at: 493 4491 493 Size: 3998 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6700 SphI G_CATG'C Cuts at: 1132 3780 4058 1132 Size: 2648 278 3774 Fragments arranged by size: 3774 2648 278 SspI AAT'ATT Cuts at: 5 52 3218 4624 5 Size: 47 3166 1406 2081 Fragments arranged by size: 3166 2081 1406 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6700 StyI C'CwwG_G Cuts at: 513 1065 2004 2408 2495 4139 513 Size: 552 939 404 87 1644 3074 Fragments arranged by size: 3074 1644 939 552 404 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2488 2644 4727 4744 4897 5082 5527 Size: 1255 908 156 2083 17 153 185 445 Cuts at: 5527 325 Size: 1498 Fragments arranged by size: 2083 1498 1255 908 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3823 3907 4946 Size: 274 80 33 51 1443 1846 84 1039 Cuts at: 4946 6658 96 Size: 1712 138 Fragments arranged by size: 1846 1712 1443 1039 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2197 2229 3742 3774 6660 6692 2197 Size: 32 1513 32 2886 32 2205 Fragments arranged by size: 2886 2205 1513 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6700 XbaI T'CTAG_A Cuts at: 4036 4036 Size: 6700 XhoI C'TCGA_G Cuts at: 1943 2376 1943 Size: 433 6267 XmaI C'CCGG_G Cuts at: 1870 4025 1870 Size: 2155 4545 XmnI GAAnn'nnTTC Cuts at: 3293 3377 3461 3881 4829 3293 Size: 84 84 420 948 5164 Fragments arranged by size: 5164 948 420 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4508 276 Size: 53 83 186 936 2974 2468 Fragments arranged by size: 2974 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI