(Circular) (Six-base) MAPSORT of: pFN21AB6232.seq Check: 2670 from: 1 to: 6733 pFN21AB6232 6733 bp With 182 enzymes: SgfI * April 15, 2010 09:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4543 278 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4076 1937 Size: 2139 4594 Acc65I G'GTAC_C Cuts at: 4054 4054 Size: 6733 AclI AA'CG_TT Cuts at: 1563 4860 5233 1563 Size: 3297 373 3063 Fragments arranged by size: 3297 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4796 6180 6448 1632 Size: 3164 1384 268 1917 Fragments arranged by size: 3164 1917 1384 268 AfeI AGC'GCT Cuts at: 2940 2940 Size: 6733 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6536 AflIII A'CryG_T Cuts at: 1203 2635 5632 6609 1203 Size: 1432 2997 977 1327 Fragments arranged by size: 2997 1432 1327 977 AgeI A'CCGG_T Cuts at: 5564 5564 Size: 6733 AhdI GACnn_n'nnGTC Cuts at: 5462 5462 Size: 6733 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2379 3170 3345 1184 Size: 1195 791 175 4572 Fragments arranged by size: 4572 1195 791 175 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2945 2977 2945 Size: 32 6701 AlwNI CAG_nnn'CTG Cuts at: 2267 3928 4476 6048 2267 Size: 1661 548 1572 2952 Fragments arranged by size: 2952 1661 1572 548 ApaI G_GGCC'C Cuts at: 2020 2067 2121 3184 2020 Size: 47 54 1063 5569 Fragments arranged by size: 5569 1063 54 47 ApaLI G'TGCA_C Cuts at: 2650 3654 4790 5946 2650 Size: 1004 1136 1156 3437 Fragments arranged by size: 3437 1156 1136 1004 ApoI r'AATT_y Cuts at: 1493 4042 4268 4420 6529 1493 Size: 2549 226 152 2109 1697 Fragments arranged by size: 2549 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5287 160 Size: 872 4255 1606 Fragments arranged by size: 4255 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2147 2595 2919 3017 3122 3401 Size: 73 117 87 448 324 98 105 279 Cuts at: 3401 3887 4058 1870 Size: 486 171 4545 Fragments arranged by size: 4545 486 448 324 279 171 117 105 98 87 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2624 2657 2624 Size: 33 6700 BamHI G'GATC_C Cuts at: 4063 4430 4063 Size: 367 6366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2272 2591 3991 4054 Size: 325 217 627 282 203 319 1400 63 Cuts at: 4054 5509 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1400 627 325 319 282 217 203 63 BanII G_rGCy'C Cuts at: 1391 2020 2058 2067 2121 2522 2852 3184 3329 Size: 629 38 9 54 401 330 332 145 Cuts at: 3329 3543 4052 1391 Size: 214 509 4072 Fragments arranged by size: 4072 629 509 401 332 330 214 145 54 38 9 BbeI G_GCGC'C Cuts at: 2073 3995 2073 Size: 1922 4811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5762 BbvCI CC'TCA_GC Cuts at: 2030 4011 2030 Size: 1981 4752 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4924 4958 1903 Size: 34 2987 34 3678 Fragments arranged by size: 3678 2987 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4625 5835 4625 Size: 1210 5523 BclI T'GATC_A Cuts at: 1548 1548 Size: 6733 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2062 3179 3802 Size: 107 122 71 631 605 390 1117 623 Cuts at: 3802 5344 136 Size: 1542 1525 Fragments arranged by size: 1542 1525 1117 631 623 605 390 122 107 71 BglII A'GATC_T Cuts at: 6728 6728 Size: 6733 BlpI GC'TnA_GC Cuts at: 2781 2879 3290 4150 2781 Size: 98 411 860 5364 Fragments arranged by size: 5364 860 411 98 Bme1580I G_kGCm'C Cuts at: 1790 2020 2067 2121 2349 2596 2654 3184 3658 Size: 230 47 54 228 247 58 530 474 Cuts at: 3658 4794 5950 1790 Size: 1136 1156 2573 Fragments arranged by size: 2573 1156 1136 530 474 247 230 228 58 54 47 BmgBI CAC'GTC Cuts at: 1318 3727 1318 Size: 2409 4324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2496 4464 5422 Size: 416 53 83 399 24 1079 1968 958 Cuts at: 5422 442 Size: 1753 Fragments arranged by size: 1968 1753 1079 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3343 3343 Size: 6733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2506 2825 3086 3116 3402 3581 4007 5393 Size: 1267 319 261 30 286 179 426 1386 Cuts at: 5393 1239 Size: 2579 Fragments arranged by size: 2579 1386 1267 426 319 286 261 179 30 Bpu10I CC'TnA_GC Cuts at: 2030 2516 3488 4011 2030 Size: 486 972 523 4752 Fragments arranged by size: 4752 972 523 486 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 4858 5723 6021 2234 Size: 2624 865 298 2946 Fragments arranged by size: 2946 2624 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2916 5396 882 Size: 617 1417 2480 2219 Fragments arranged by size: 2480 2219 1417 617 BsaAI yAC'GTr Cuts at: 493 1204 2542 4524 493 Size: 711 1338 1982 2702 Fragments arranged by size: 2702 1982 1338 711 BsaBI GATnn'nnATC Cuts at: 2454 6727 2454 Size: 4273 2460 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2143 2169 3426 Size: 53 83 186 936 537 73 26 1257 Cuts at: 3426 3597 3992 4540 4922 5557 275 Size: 171 395 548 382 635 1451 Fragments arranged by size: 1451 1257 936 635 548 537 395 382 186 171 83 73 53 26 BsaWI w'CCGG_w Cuts at: 2022 2379 3846 5166 5564 5838 5985 6396 2022 Size: 357 1467 1320 398 274 147 411 2359 Fragments arranged by size: 2359 1467 1320 411 398 357 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4010 4040 1178 Size: 30 165 30 2607 30 3871 Fragments arranged by size: 3871 2607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2780 3578 1186 Size: 1594 798 4341 Fragments arranged by size: 4341 1594 798 BseYI C'CCAG_C Cuts at: 2506 3243 3607 3902 5936 6432 2506 Size: 737 364 295 2034 496 2807 Fragments arranged by size: 2807 2034 737 496 364 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2458 4183 4944 5972 1540 Size: 275 186 457 1725 761 1028 2301 Fragments arranged by size: 2301 1725 1028 761 457 275 186 BsiHKAI G_wGCw'C Cuts at: 2522 2550 2654 2760 3329 3645 3658 4794 4879 Size: 28 104 106 569 316 13 1136 85 Cuts at: 4879 5950 2522 Size: 1071 3305 Fragments arranged by size: 3305 1136 1071 569 316 106 104 85 28 13 BsmI GAATG_Cn' Cuts at: 4244 4337 4244 Size: 93 6640 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2058 2067 2121 2349 2522 2550 Size: 399 230 38 9 54 228 173 28 Cuts at: 2550 2596 2654 2760 2852 3184 3329 3543 3645 Size: 46 58 106 92 332 145 214 102 Cuts at: 3645 3658 4052 4794 4879 5950 1391 Size: 13 394 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 394 332 230 228 214 173 145 106 102 92 85 58 54 46 38 28 13 9 BspEI T'CCGG_A Cuts at: 2022 2379 6396 2022 Size: 357 4017 2359 Fragments arranged by size: 4017 2359 357 BspHI T'CATG_A Cuts at: 4622 6352 4622 Size: 1730 5003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4088 844 Size: 3244 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4620 1394 Size: 429 2797 3507 Fragments arranged by size: 3507 2797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3831 5228 5402 65 Size: 1183 2583 1397 174 1396 Fragments arranged by size: 2583 1397 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2997 3630 5377 5564 1700 Size: 110 1187 633 1747 187 2869 Fragments arranged by size: 2869 1747 1187 633 187 110 BsrGI T'GTAC_A Cuts at: 96 2943 96 Size: 2847 3886 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6733 BssSI C'ACGA_G Cuts at: 3575 3998 4793 5805 3575 Size: 423 795 1012 4503 Fragments arranged by size: 4503 1012 795 423 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6733 BstBI TT'CG_AA Cuts at: 5537 5537 Size: 6733 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6733 BstYI r'GATC_y Cuts at: 1972 4063 4430 4815 4832 6284 6370 6382 6728 Size: 2091 367 385 17 1452 86 12 346 Cuts at: 6728 1972 Size: 1977 Fragments arranged by size: 2091 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2621 2802 3612 3820 3870 513 Size: 552 1556 181 810 208 50 3376 Fragments arranged by size: 3376 1556 810 552 208 181 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2898 487 Size: 683 56 1672 4322 Fragments arranged by size: 4322 1672 683 56 BtsI GCAGTG_nn' Cuts at: 3418 3597 4249 5043 5063 3418 Size: 179 652 794 20 5088 Fragments arranged by size: 5088 794 652 179 20 ClaI AT'CG_AT Cuts at: 1554 2454 1554 Size: 900 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6698 DraI TTT'AAA Cuts at: 4037 4037 Size: 6733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3736 5740 809 Size: 507 2420 2004 1802 Fragments arranged by size: 2420 2004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2799 3963 Size: 54 1425 50 275 18 217 752 1164 Cuts at: 3963 4180 5069 6698 8 Size: 217 889 1629 43 Fragments arranged by size: 1629 1425 1164 889 752 275 217 217 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4180 1537 Size: 275 2368 4090 Fragments arranged by size: 4090 2368 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3215 3845 4663 1353 Size: 1862 630 818 3423 Fragments arranged by size: 3423 1862 818 630 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3194 3649 5320 5694 5840 387 Size: 1064 138 1605 455 1671 374 146 1280 Fragments arranged by size: 1671 1605 1280 1064 455 374 146 138 EcoICRI GAG'CTC Cuts at: 2520 3327 2520 Size: 807 5926 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2506 2825 3086 3116 3402 3581 4007 Size: 393 874 319 261 30 286 179 426 Cuts at: 4007 4796 5393 6180 6448 1239 Size: 789 597 787 268 1524 Fragments arranged by size: 1524 874 789 787 597 426 393 319 286 268 261 179 30 EcoNI CCTnn'n_nnAGG Cuts at: 3212 3790 3212 Size: 578 6155 EcoO109I rG'GnC_Cy Cuts at: 2017 2117 2991 3136 3604 3925 2017 Size: 100 874 145 468 321 4825 Fragments arranged by size: 4825 874 468 321 145 100 EcoRI G'AATT_C Cuts at: 4042 4420 4042 Size: 378 6355 EcoRV GAT'ATC Cuts at: 2452 2452 Size: 6733 FspI TGC'GCA Cuts at: 5239 5239 Size: 6733 HaeII r_GCGC'y Cuts at: 1678 2073 2942 3095 3368 3995 5880 1678 Size: 395 869 153 273 627 1885 2531 Fragments arranged by size: 2531 1885 869 627 395 273 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3793 3825 5374 5406 5448 Size: 32 135 32 2221 32 1549 32 42 Cuts at: 5448 5480 1373 Size: 32 2626 Fragments arranged by size: 2626 2221 1549 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2179 2863 4077 4323 669 Size: 988 281 241 684 1214 246 3079 Fragments arranged by size: 3079 1214 988 684 281 246 241 HindIII A'AGCT_T Cuts at: 748 748 Size: 6733 HpaI GTT'AAC Cuts at: 4323 4323 Size: 6733 KasI G'GCGC_C Cuts at: 2069 3991 2069 Size: 1922 4811 KpnI G_GTAC'C Cuts at: 4058 4058 Size: 6733 MfeI C'AATT_G Cuts at: 4332 4332 Size: 6733 MluI A'CGCG_T Cuts at: 6609 6609 Size: 6733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5847 6031 1305 Size: 4542 184 2007 Fragments arranged by size: 4542 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3965 10 Size: 54 1425 343 2133 2778 Fragments arranged by size: 2778 2133 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 3675 4691 5050 5209 518 Size: 648 1201 1308 1016 359 159 2042 Fragments arranged by size: 2042 1308 1201 1016 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3496 4149 4826 5974 6219 1606 Size: 1890 653 677 1148 245 2120 Fragments arranged by size: 2120 1890 1148 677 653 245 NaeI GCC'GGC Cuts at: 2999 2999 Size: 6733 NarI GG'CG_CC Cuts at: 2070 3992 2070 Size: 1922 4811 NcoI C'CATG_G Cuts at: 513 1065 3820 3870 513 Size: 552 2755 50 3376 Fragments arranged by size: 3376 2755 552 50 NdeI CA'TA_TG Cuts at: 387 387 Size: 6733 NgoMIV G'CCGG_C Cuts at: 2997 2997 Size: 6733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6733 NotI GC'GGCC_GC Cuts at: 4180 4180 Size: 6733 NspI r_CATG'y Cuts at: 1132 1229 3151 4091 5636 1132 Size: 97 1922 940 1545 2229 Fragments arranged by size: 2229 1922 1545 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6733 PciI A'CATG_T Cuts at: 5632 5632 Size: 6733 PflMI CCAn_nnn'nTGG Cuts at: 1108 3555 1108 Size: 2447 4286 PfoI T'CCnGG_A Cuts at: 2522 3063 3213 2522 Size: 541 150 6042 Fragments arranged by size: 6042 541 150 PmeI GTTT'AAAC Cuts at: 4037 4037 Size: 6733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4802 4834 6315 6347 4802 Size: 32 1481 32 5188 Fragments arranged by size: 5188 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3136 3604 3136 Size: 468 6265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6733 PsiI TTA'TAA Cuts at: 4303 4303 Size: 6733 PspOMI G'GGCC_C Cuts at: 2016 2063 2117 3180 2016 Size: 47 54 1063 5569 Fragments arranged by size: 5569 1063 54 47 PspXI vC'TCGA_Gb Cuts at: 3401 3401 Size: 6733 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3179 3211 3179 Size: 32 6701 PstI C_TGCA'G Cuts at: 830 2877 4085 830 Size: 2047 1208 3478 Fragments arranged by size: 3478 2047 1208 PvuI CG_AT'CG Cuts at: 2001 2458 2001 Size: 457 6276 PvuII CAG'CTG Cuts at: 3496 3496 Size: 6733 SacI G_AGCT'C Cuts at: 2522 3329 2522 Size: 807 5926 SalI G'TCGA_C Cuts at: 1936 4075 1936 Size: 2139 4594 SanDI GG'GwC_CC Cuts at: 3604 3604 Size: 6733 SbfI CC_TGCA'GG Cuts at: 4085 4085 Size: 6733 ScaI AGT'ACT Cuts at: 3101 4981 6693 3101 Size: 1880 1712 3141 Fragments arranged by size: 3141 1880 1712 SexAI A'CCwGG_T Cuts at: 2698 2698 Size: 6733 SfcI C'TryA_G Cuts at: 826 1045 2689 2873 4081 5216 5897 6088 826 Size: 219 1644 184 1208 1135 681 191 1471 Fragments arranged by size: 1644 1471 1208 1135 681 219 191 184 SfiI GGCCn_nnn'nGGCC Cuts at: 3179 3179 Size: 6733 SfoI GGC'GCC Cuts at: 2071 3993 2071 Size: 1922 4811 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6733 SmaI CCC'GGG Cuts at: 1872 2149 2597 4060 1872 Size: 277 448 1463 4545 Fragments arranged by size: 4545 1463 448 277 SmlI C'TyrA_G Cuts at: 820 1017 1943 2213 2919 3122 3401 4837 5738 Size: 197 926 270 706 203 279 1436 901 Cuts at: 5738 6000 820 Size: 262 1553 Fragments arranged by size: 1553 1436 926 901 706 279 270 262 203 197 SnaBI TAC'GTA Cuts at: 493 4524 493 Size: 4031 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6733 SphI G_CATG'C Cuts at: 1132 3151 4091 1132 Size: 2019 940 3774 Fragments arranged by size: 3774 2019 940 SrfI GCCC'GGGC Cuts at: 2597 2597 Size: 6733 SspI AAT'ATT Cuts at: 5 52 4657 5 Size: 47 4605 2081 Fragments arranged by size: 4605 2081 47 StuI AGG'CCT Cuts at: 1517 2736 3344 1517 Size: 1219 608 4906 Fragments arranged by size: 4906 1219 608 StyI C'CwwG_G Cuts at: 513 1065 3511 3820 3870 4172 513 Size: 552 2446 309 50 302 3074 Fragments arranged by size: 3074 2446 552 309 302 50 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3673 4760 4777 4930 5115 5560 325 Size: 1255 2093 1087 17 153 185 445 1498 Fragments arranged by size: 2093 1498 1255 1087 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2943 3099 4979 Size: 274 80 33 51 1443 966 156 1880 Cuts at: 4979 6691 96 Size: 1712 138 Fragments arranged by size: 1880 1712 1443 966 274 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4010 4042 6693 6725 4010 Size: 32 2651 32 4018 Fragments arranged by size: 4018 2651 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6733 XbaI T'CTAG_A Cuts at: 4069 4069 Size: 6733 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2716 3556 2716 Size: 840 5893 XhoI C'TCGA_G Cuts at: 1943 2919 3122 3401 1943 Size: 976 203 279 5275 Fragments arranged by size: 5275 976 279 203 XmaI C'CCGG_G Cuts at: 1870 2147 2595 4058 1870 Size: 277 448 1463 4545 Fragments arranged by size: 4545 1463 448 277 XmnI GAAnn'nnTTC Cuts at: 4862 4862 Size: 6733 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4541 276 Size: 53 83 186 936 3007 2468 Fragments arranged by size: 3007 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI NruI NsiI PacI PmlI RsrII SacII SapI SwaI