(Circular) (Six-base) MAPSORT of: pFN21AE0764.seq Check: 403 from: 1 to: 6016 pFN21AE0764 6016 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 2720 4143 4516 1563 Size: 1157 1423 373 3063 Fragments arranged by size: 3063 1423 1157 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 2718 2949 4079 5463 5731 1632 Size: 599 487 231 1130 1384 268 1917 Fragments arranged by size: 1917 1384 1130 599 487 268 231 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2391 4915 5892 1203 Size: 1188 2524 977 1327 Fragments arranged by size: 2524 1327 1188 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2323 1184 Size: 1139 4877 AlwNI CAG_nnn'CTG Cuts at: 3142 3759 5331 3142 Size: 617 1572 3827 Fragments arranged by size: 3827 1572 617 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 3307 3325 3551 3703 5812 1493 Size: 1814 18 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 3341 1870 Size: 73 1398 4545 Fragments arranged by size: 4545 1398 73 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3337 4792 618 Size: 325 217 627 1550 1455 1842 Fragments arranged by size: 1842 1550 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3335 1391 Size: 1944 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2624 928 Size: 971 725 4320 Fragments arranged by size: 4320 971 725 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2116 6011 2116 Size: 3895 2121 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 4077 5233 1790 Size: 2287 1156 2573 Fragments arranged by size: 2573 2287 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6016 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3747 4705 442 Size: 416 53 83 399 24 2330 958 1753 Fragments arranged by size: 2330 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2088 2088 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4676 1239 Size: 3437 2579 Bpu10I CC'TnA_GC Cuts at: 2177 2177 Size: 6016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2058 3260 4141 5006 5304 2058 Size: 1202 881 865 298 2770 Fragments arranged by size: 2770 1202 881 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4679 882 Size: 617 3180 2219 Fragments arranged by size: 3180 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2936 2966 1178 Size: 30 165 30 1533 30 4228 Fragments arranged by size: 4228 1533 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2502 2973 1186 Size: 927 389 471 4229 Fragments arranged by size: 4229 927 471 389 BseYI C'CCAG_C Cuts at: 2567 2624 5219 5715 2567 Size: 57 2595 496 2868 Fragments arranged by size: 2868 2595 496 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 2526 1163 Size: 573 663 127 4653 Fragments arranged by size: 4653 663 573 127 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2038 4077 4162 5233 2038 Size: 2039 85 1071 2821 Fragments arranged by size: 2821 2039 1071 85 BsmI GAATG_Cn' Cuts at: 2312 3527 3620 2312 Size: 1215 93 4708 Fragments arranged by size: 4708 1215 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 3335 4077 4162 5233 1391 Size: 399 248 1297 742 85 1071 2174 Fragments arranged by size: 2174 1297 1071 742 399 248 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 2857 3189 3905 5635 2857 Size: 332 716 1730 3238 Fragments arranged by size: 3238 1730 716 332 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2283 3371 844 Size: 1439 1088 3489 Fragments arranged by size: 3489 1439 1088 BsrBI CCG'CTC Cuts at: 1394 1823 3903 1394 Size: 429 2080 3507 Fragments arranged by size: 3507 2080 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 2952 96 Size: 2856 3160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2116 2479 3346 3713 4098 4115 5567 5653 Size: 144 363 867 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 867 385 367 363 346 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5464 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 2038 3532 4326 4346 2038 Size: 1494 794 20 3708 Fragments arranged by size: 3708 1494 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2923 5023 809 Size: 507 1607 2100 1802 Fragments arranged by size: 2100 1802 1607 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3123 3463 4352 Size: 54 1425 50 275 18 1293 340 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1293 889 340 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2602 2907 3946 1353 Size: 1249 305 1039 3423 Fragments arranged by size: 3423 1249 1039 305 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2718 2949 4079 4676 5463 5731 Size: 393 599 487 231 1130 597 787 268 Cuts at: 5731 1239 Size: 1524 Fragments arranged by size: 1524 1130 787 599 597 487 393 268 231 EcoO109I rG'GnC_Cy Cuts at: 2773 2773 Size: 6016 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 EcoRV GAT'ATC Cuts at: 2106 2106 Size: 6016 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 5163 1678 Size: 3485 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2323 2355 2645 2677 2839 Size: 32 135 32 751 32 290 32 162 Cuts at: 2839 2871 2883 2915 4657 4689 4731 4763 1373 Size: 32 12 32 1742 32 42 32 2626 Fragments arranged by size: 2626 1742 751 290 162 135 42 32 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 2272 2680 3360 3606 669 Size: 988 281 334 408 680 246 3079 Fragments arranged by size: 3079 988 680 408 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 2680 3606 2680 Size: 926 5090 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2075 5130 5314 1305 Size: 770 3055 184 2007 Fragments arranged by size: 3055 2007 770 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3125 10 Size: 54 1425 343 1293 2901 Fragments arranged by size: 2901 1425 1293 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2305 3103 3974 4333 4492 518 Size: 648 1139 798 871 359 159 2042 Fragments arranged by size: 2042 1139 871 798 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2369 3229 3432 4109 5257 5502 1606 Size: 763 860 203 677 1148 245 2120 Fragments arranged by size: 2120 1148 860 763 677 245 203 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 2324 3374 4919 1132 Size: 97 1095 1050 1545 2229 Fragments arranged by size: 2229 1545 1095 1050 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 3142 1108 Size: 2034 3982 PfoI T'CCnGG_A Cuts at: 3207 3207 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PstI C_TGCA'G Cuts at: 830 3368 830 Size: 2538 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2369 2369 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 3364 4499 5180 5371 826 Size: 219 2319 1135 681 191 1471 Fragments arranged by size: 2319 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2073 3275 4120 5021 5283 820 Size: 197 926 130 1202 845 901 262 1553 Fragments arranged by size: 1553 1202 926 901 845 262 197 130 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 2324 3374 1132 Size: 1192 1050 3774 Fragments arranged by size: 3774 1192 1050 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2137 3455 513 Size: 552 1072 1318 3074 Fragments arranged by size: 3074 1318 1072 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 2581 2638 3009 4043 4060 4213 Size: 1255 611 390 57 371 1034 17 153 Cuts at: 4213 4398 4843 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1034 611 445 390 371 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2952 4262 Size: 274 80 33 51 1443 249 726 1310 Cuts at: 4262 5974 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1310 726 274 249 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5976 6008 5976 Size: 32 5984 Tth111I GACn'n_nGTC Cuts at: 1316 2530 1316 Size: 1214 4802 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 2195 4145 2195 Size: 1950 4066 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI