Restriction map for
pFN21AB5946
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaII AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BglI BlnI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiWI BsmBI BspDI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KspI MaeII MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StyI
SwaI TfiI Tsp45I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 146 GGTACC G^GTACC
AflIII 223 ACATGT A^C[GA][CT]GT
AlwNI 14 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 389 GGGCCC GGGCC^C
Asp718I 146 GGTACC G^GTACC
AvaI 703 CCCGAG C^[CT]CG[GA]G
BalI 645 TGGCCA TGG^CCA
BamHI 1063 GGATCC G^GATCC
BanI 146 GGTACC G^G[CT][GA]CC
BbsI 3 GTCTTC GAAGAC(2/6)
BclI 1191 TGATCA T^GATCA
BglII 102 AGATCT A^GATCT
BlpI 876 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 876 GCTGAGC GC^T[ACGT]AGC
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaBI 97 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 160 CTCCTC GAGGAG(10/8)
BsiEI 336 CGACCG CG[GA][CT]^CG
BsmI 341 GAATGC GAATGC(1/-1)
BsoBI 703 CCCGAG C^[CT]CG[GA]G
Bsp1407I 374 TGTACA T^GTACA
BspHI 1142 TCATGA T^CATGA
BspLU11I 223 ACATGT A^CATGT
BsrGI 374 TGTACA T^GTACA
BstEII 631 GGTTACC G^GT[ACGT]ACC
BstPI 631 GGTTACC G^GT[ACGT]ACC
CelII 876 GCTGAGC GC^T[ACGT]AGC
DraIII 1314 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 645 TGGCCA [CT]^GGCC[GA]
EcoO65I 631 GGTTACC G^GT[ACGT]ACC
FauI 1329 CCCGC CCCGC(4/6)
FbaI 1191 TGATCA T^GATCA
KpnI 146 GGTACC GGTAC^C
MamI 97 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 645 TGGCCA TGG^CCA
MscI 645 TGGCCA TGG^CCA
MspA1I 1264 CAGCTG C[AC]G^C[GT]G
NdeI 641 CATATG CA^TATG
PshAI 568 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1264 CAGCTG CAG^CTG
RcaI 1142 TCATGA T^CATGA
SapI 109 GAAGAGC GCTCTTC(1/4)
SfcI 557 CTACAG C^T[GA][CT]AG
SphI 1470 GCATGC GCATG^C
SspBI 374 TGTACA T^GTACA
StuI 525 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 1298 CCGC CCGC(-3/-1)
1330 CCGC
AcsI 1216 AAATTC [GA]^AATT[CT]
1345 AAATTT
AflII 433 CTTAAG C^TTAAG
967 CTTAAG
ApoI 1216 AAATTC [GA]^AATT[CT]
1345 AAATTT
BcnI 660 CCGGG CC^[GC]GG
1380 CCGGG
BfrI 433 CTTAAG C^TTAAG
967 CTTAAG
BsmFI 119 GTCCC GGGAC(10/14)
575 GTCCC
BstXI 636 CCAAACATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1067 CCATCTTCCTGG
BstYI 102 AGATCT [GA]^GATC[CT]
1063 GGATCC
DraI 39 TTTAAA TTT^AAA
73 TTTAAA
DraII 388 AGGGCCC [GA]G^G[ACGT]CC[CT]
389 GGGCCCT
EarI 919 CTCTTC CTCTTC(1/4)
109 GAAGAG
EcoO109I 388 AGGGCCC [GA]G^G[ACGT]CC[CT]
389 GGGCCCT
Ksp632I 919 CTCTTC CTCTTC(1/4)
109 GAAGAG
MflI 102 AGATCT [GA]^GATC[CT]
1063 GGATCC
NciI 660 CCGGG CC^[GC]GG
1380 CCGGG
NspI 223 ACATGT [GA]CATG^[CT]
1470 GCATGC
SacI 747 GAGCTC GAGCT^C
1012 GAGCTC
SfaNI 761 GCATC GCATC(5/9)
1111 GCATC
XhoII 102 AGATCT [GA]^GATC[CT]
1063 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AfaI AluI AlwI AspHI BanII
BbvI BfaI BmyI BpmI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SmlI
TaqI Tru9I TseI Tsp509I TthHB8I