(Circular) (Six-base) MAPSORT of: pFN21AB5946.seq Check: 7702 from: 1 to: 6190 pFN21AB5946 6190 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 2151 3511 2151 Size: 1360 4830 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2420 2947 2984 3426 4253 5637 5905 1632 Size: 788 527 37 442 827 1384 268 1917 Fragments arranged by size: 1917 1384 827 788 527 442 268 37 AflII C'TTAA_G Cuts at: 820 1017 2438 2972 820 Size: 197 1421 534 4038 Fragments arranged by size: 4038 1421 534 197 AflIII A'CryG_T Cuts at: 1203 2228 5089 6066 1203 Size: 1025 2861 977 1327 Fragments arranged by size: 2861 1327 1025 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2627 1184 Size: 1443 4747 AlwNI CAG_nnn'CTG Cuts at: 2024 3933 5505 2024 Size: 1909 1572 2709 Fragments arranged by size: 2709 1909 1572 ApaI G_GGCC'C Cuts at: 2398 2398 Size: 6190 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 3221 3350 3499 3725 3877 5986 1493 Size: 1728 129 149 226 152 2109 1697 Fragments arranged by size: 2109 1728 1697 226 152 149 129 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2708 3515 1870 Size: 73 765 807 4545 Fragments arranged by size: 4545 807 765 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2215 2248 2215 Size: 33 6157 BamHI G'GATC_C Cuts at: 3068 3520 3887 3068 Size: 452 367 5371 Fragments arranged by size: 5371 452 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 3511 4966 618 Size: 325 217 627 364 1360 1455 1842 Fragments arranged by size: 1842 1455 1360 627 364 325 217 BanII G_rGCy'C Cuts at: 1391 2166 2398 2756 3021 3509 1391 Size: 775 232 358 265 488 4072 Fragments arranged by size: 4072 775 488 358 265 232 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 928 Size: 971 102 5117 Fragments arranged by size: 5117 971 102 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 3196 1548 Size: 1648 4542 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2107 6185 2107 Size: 4078 2112 BlpI GC'TnA_GC Cuts at: 2882 3607 2882 Size: 725 5465 Bme1580I G_kGCm'C Cuts at: 1790 2398 3447 4251 5407 1790 Size: 608 1049 804 1156 2573 Fragments arranged by size: 2573 1156 1049 804 608 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2034 3096 3921 Size: 416 53 83 399 24 617 1062 825 Cuts at: 3921 4879 442 Size: 958 1753 Fragments arranged by size: 1753 1062 958 825 617 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3152 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2742 3031 3173 4850 1239 Size: 1503 289 142 1677 2579 Fragments arranged by size: 2579 1677 1503 289 142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2799 4315 5180 5478 2799 Size: 1516 865 298 3511 Fragments arranged by size: 3511 1516 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 2106 6184 2106 Size: 4078 2112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3997 4379 5014 275 Size: 53 83 186 936 2464 382 635 1451 Fragments arranged by size: 2464 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2127 2157 2507 2537 2740 Size: 30 165 30 724 30 350 30 203 Cuts at: 2740 2770 1178 Size: 30 4598 Fragments arranged by size: 4598 724 350 203 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2156 1186 Size: 970 5220 BseYI C'CCAG_C Cuts at: 5393 5889 5393 Size: 496 5694 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5617 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2344 3640 4401 5429 1540 Size: 275 186 343 1296 761 1028 2301 Fragments arranged by size: 2301 1296 1028 761 343 275 186 BsiHKAI G_wGCw'C Cuts at: 2756 2888 3021 3234 4251 4336 5407 2756 Size: 132 133 213 1017 85 1071 3539 Fragments arranged by size: 3539 1071 1017 213 133 132 85 BsmI GAATG_Cn' Cuts at: 2352 3701 3794 2352 Size: 1349 93 4748 Fragments arranged by size: 4748 1349 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2398 2756 2888 3021 3234 3447 Size: 399 376 232 358 132 133 213 213 Cuts at: 3447 3509 4251 4336 5407 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 358 232 213 213 133 132 85 62 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 3147 4079 5809 3147 Size: 932 1730 3528 Fragments arranged by size: 3528 1730 932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3545 844 Size: 2701 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4685 4859 65 Size: 1183 3437 174 1396 Fragments arranged by size: 3437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4834 5021 1700 Size: 110 3024 187 2869 Fragments arranged by size: 3024 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2379 96 Size: 2283 3907 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 4250 5262 4250 Size: 1012 5178 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 2636 1184 Size: 1452 4738 BstXI CCAn_nnnn'nTGG Cuts at: 2648 3079 2648 Size: 431 5759 BstYI r'GATC_y Cuts at: 1972 2107 3068 3520 3887 4272 4289 5741 5827 Size: 135 961 452 367 385 17 1452 86 Cuts at: 5827 5839 6185 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 961 452 385 367 346 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5638 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 3706 4500 4520 3706 Size: 794 20 5376 Fragments arranged by size: 5376 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 2046 2080 3494 2046 Size: 34 1414 4742 Fragments arranged by size: 4742 1414 34 DraIII CAC_nnn'GTG Cuts at: 3324 3324 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2650 3637 4526 Size: 54 1425 50 275 18 820 987 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1425 987 889 820 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2930 4120 1353 Size: 756 821 1190 3423 Fragments arranged by size: 3423 1190 821 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4777 5151 5297 387 Size: 1064 138 3188 374 146 1280 Fragments arranged by size: 3188 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2754 3019 2754 Size: 265 5925 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2420 2742 2947 2984 3031 3173 3426 Size: 393 788 322 205 37 47 142 253 Cuts at: 3426 4253 4850 5637 5905 1239 Size: 827 597 787 268 1524 Fragments arranged by size: 1524 827 788 787 597 393 322 268 253 205 142 47 37 EcoO109I rG'GnC_Cy Cuts at: 2394 2395 2394 Size: 1 6189 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2740 2772 4831 Size: 32 135 32 557 32 579 32 2059 Cuts at: 4831 4863 4905 4937 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2059 579 557 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3780 669 Size: 988 281 1596 246 3079 Fragments arranged by size: 3079 1596 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 2155 3515 2155 Size: 1360 4830 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2293 5304 5488 1305 Size: 988 3011 184 2007 Fragments arranged by size: 3011 2007 988 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2652 10 Size: 54 1425 343 820 3548 Fragments arranged by size: 3548 1425 820 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2603 2609 3395 3432 3467 4148 4507 Size: 648 1437 6 786 37 35 681 359 Cuts at: 4507 4666 518 Size: 159 2042 Fragments arranged by size: 2042 1437 786 681 648 359 159 37 35 6 MspA1I CmG'CkG Cuts at: 1606 3271 3606 4283 5431 5676 1606 Size: 1665 335 677 1148 245 2120 Fragments arranged by size: 2120 1665 1148 677 335 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5638 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 3930 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2232 3479 3548 5093 1132 Size: 97 1003 1247 69 1545 2229 Fragments arranged by size: 2229 1545 1247 1003 97 69 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 2228 5089 2228 Size: 2861 3329 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6190 PfoI T'CCnGG_A Cuts at: 3285 3285 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2577 1613 Size: 964 5226 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 2394 2394 Size: 6190 PstI C_TGCA'G Cuts at: 830 3542 830 Size: 2712 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 3271 3271 Size: 6190 SacI G_AGCT'C Cuts at: 2756 3021 2756 Size: 265 5925 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2109 2109 Size: 6190 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2562 3538 4673 5354 5545 826 Size: 219 1517 976 1135 681 191 1471 Fragments arranged by size: 1517 1471 1135 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 3517 1872 Size: 1645 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 2814 2972 4294 5195 5457 Size: 197 926 495 376 158 1322 901 262 Cuts at: 5457 820 Size: 1553 Fragments arranged by size: 1553 1322 926 901 495 376 262 197 158 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 3479 3548 1132 Size: 2347 69 3774 Fragments arranged by size: 3774 2347 69 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 2532 1517 Size: 1015 5175 StyI C'CwwG_G Cuts at: 513 1065 3629 513 Size: 552 2564 3074 Fragments arranged by size: 3074 2564 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2358 4217 4234 4387 4572 5017 325 Size: 1255 778 1859 17 153 185 445 1498 Fragments arranged by size: 1859 1498 1255 778 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 4436 6148 Size: 274 80 33 51 1443 402 2057 1712 Cuts at: 6148 96 Size: 138 Fragments arranged by size: 2057 1712 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2507 2539 6150 6182 2127 Size: 32 348 32 3611 32 2135 Fragments arranged by size: 3611 2135 348 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 3515 1870 Size: 1645 4545 XmnI GAAnn'nnTTC Cuts at: 4319 4319 Size: 6190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI