Restriction map for
pFN21AB5946
(Linear) (Six-base) MAPSORT of: KIBB5946.seq Check: 959 from: 1 to: 1485
KIBB5946.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 146 1485
Size: 146 1339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 942 979 1421 1485
Size: 415 527 37 442 64
Fragments arranged by size:
527 442 415 64 37
AflII C'TTAA_G
Cuts at: 0 433 967 1485
Size: 433 534 518
Fragments arranged by size:
534 518 433
AflIII A'CryG_T
Cuts at: 0 223 1485
Size: 223 1262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 622 1485
Size: 622 863
AlwNI CAG_nnn'CTG
Cuts at: 0 19 1485
Size: 19 1466
ApaI G_GGCC'C
Cuts at: 0 393 1485
Size: 393 1092
ApoI r'AATT_y
Cuts at: 0 1216 1345 1485
Size: 1216 129 140
Fragments arranged by size:
1216 140 129
AvaI C'yCGr_G
Cuts at: 0 703 1485
Size: 703 782
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 210 243 1485
Size: 210 33 1242
Fragments arranged by size:
1242 210 33
BamHI G'GATC_C
Cuts at: 0 1063 1485
Size: 1063 422
BanI G'GyrC_C
Cuts at: 0 146 1485
Size: 146 1339
BanII G_rGCy'C
Cuts at: 0 161 393 751 1016 1485
Size: 161 232 358 265 469
Fragments arranged by size:
469 358 265 232 161
BclI T'GATC_A
Cuts at: 0 1191 1485
Size: 1191 294
BglII A'GATC_T
Cuts at: 0 102 1485
Size: 102 1383
BlpI GC'TnA_GC
Cuts at: 0 877 1485
Size: 877 608
Bme1580I G_kGCm'C
Cuts at: 0 393 1442 1485
Size: 393 1049 43
Fragments arranged by size:
1049 393 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 29 1091 1485
Size: 29 1062 394
Fragments arranged by size:
1062 394 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1485
Size: 1147 338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1026 1168 1485
Size: 737 289 142 317
Fragments arranged by size:
737 317 289 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1485
Size: 794 691
BsaBI GATnn'nnATC
Cuts at: 0 101 1485
Size: 101 1384
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 122 152 502 532 735 765 1485
Size: 122 30 350 30 203 30 720
Fragments arranged by size:
720 350 203 122 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 1485
Size: 151 1334
BsiEI CG_ry'CG
Cuts at: 0 339 1485
Size: 339 1146
BsiHKAI G_wGCw'C
Cuts at: 0 751 883 1016 1229 1485
Size: 751 132 133 213 256
Fragments arranged by size:
751 256 213 133 132
BsmI GAATG_Cn'
Cuts at: 0 347 1485
Size: 347 1138
Bsp1286I G_dGCh'C
Cuts at: 0 161 393 751 883 1016 1229 1442 1485
Size: 161 232 358 132 133 213 213 43
Fragments arranged by size:
358 232 213 213 161 133 132 43
BspHI T'CATG_A
Cuts at: 0 1142 1485
Size: 1142 343
BsrGI T'GTAC_A
Cuts at: 0 374 1485
Size: 374 1111
BstEII G'GTnAC_C
Cuts at: 0 631 1485
Size: 631 854
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 1074 1485
Size: 643 431 411
Fragments arranged by size:
643 431 411
BstYI r'GATC_y
Cuts at: 0 102 1063 1485
Size: 102 961 422
Fragments arranged by size:
961 422 102
DraI TTT'AAA
Cuts at: 0 41 75 1485
Size: 41 34 1410
Fragments arranged by size:
1410 41 34
DraIII CAC_nnn'GTG
Cuts at: 0 1319 1485
Size: 1319 166
EaeI y'GGCC_r
Cuts at: 0 645 1485
Size: 645 840
EarI CTCTTCn'nnn_
Cuts at: 0 104 925 1485
Size: 104 821 560
Fragments arranged by size:
821 560 104
EcoICRI GAG'CTC
Cuts at: 0 749 1014 1485
Size: 749 265 471
Fragments arranged by size:
749 471 265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 737 942 979 1026 1168 1421 1485
Size: 415 322 205 37 47 142 253 64
Fragments arranged by size:
415 322 253 205 142 64 47 37
EcoO109I rG'GnC_Cy
Cuts at: 0 389 390 1485
Size: 389 1 1095
Fragments arranged by size:
1095 389 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 735 767 1485
Size: 124 32 579 32 718
Fragments arranged by size:
718 579 124 32 32
KpnI G_GTAC'C
Cuts at: 0 150 1485
Size: 150 1335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 1485
Size: 288 1197
MscI TGG'CCA
Cuts at: 0 647 1485
Size: 647 838
MslI CAynn'nnrTG
Cuts at: 0 598 604 1390 1427 1462 1485
Size: 598 6 786 37 35 23
Fragments arranged by size:
786 598 37 35 23 6
MspA1I CmG'CkG
Cuts at: 0 1266 1485
Size: 1266 219
NdeI CA'TA_TG
Cuts at: 0 642 1485
Size: 642 843
NspI r_CATG'y
Cuts at: 0 227 1474 1485
Size: 227 1247 11
Fragments arranged by size:
1247 227 11
PciI A'CATG_T
Cuts at: 0 223 1485
Size: 223 1262
PfoI T'CCnGG_A
Cuts at: 0 1280 1485
Size: 1280 205
PshAI GACnn'nnGTC
Cuts at: 0 572 1485
Size: 572 913
PspOMI G'GGCC_C
Cuts at: 0 389 1485
Size: 389 1096
PvuII CAG'CTG
Cuts at: 0 1266 1485
Size: 1266 219
SacI G_AGCT'C
Cuts at: 0 751 1016 1485
Size: 751 265 469
Fragments arranged by size:
751 469 265
SapI GCTCTTCn'nnn_
Cuts at: 0 104 1485
Size: 104 1381
SfcI C'TryA_G
Cuts at: 0 557 1485
Size: 557 928
SmlI C'TyrA_G
Cuts at: 0 433 809 967 1485
Size: 433 376 158 518
Fragments arranged by size:
518 433 376 158
SphI G_CATG'C
Cuts at: 0 1474 1485
Size: 1474 11
StuI AGG'CCT
Cuts at: 0 527 1485
Size: 527 958
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 353 1485
Size: 353 1132
TatI w'GTAC_w
Cuts at: 0 374 1485
Size: 374 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 122 154 502 534 1485
Size: 122 32 348 32 951
Fragments arranged by size:
951 348 122 32 32
Enzymes that do cut:
Acc65I AcuI AflII AflIII AlfI AlwNI ApaI ApoI
AvaI BaeI BamHI BanI BanII BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaBI BsaXI BseRI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrGI BstEII BstXI
BstYI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I
Hin4I KpnI MmeI MscI MslI MspA1I NdeI NspI
PciI PfoI PshAI PspOMI PvuII SacI SapI SfcI
SmlI SphI StuI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BmgBI BmtI Bpu10I
BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StyI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI