Restriction map for
pFN21AE1198
##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII BalI BamHI BclI BglII
BlpI Bpu1102I BsaBI BsaI BsiWI
BsmBI BspDI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstZ17I CelII
Cfr10I ClaI DraI DrdI Eam1105I
EcoRV EcoT22I FbaI FseI FspI
HpaI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NheI NotI NsiI PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I RcaI SacII SalI SapI
SbfI SfiI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1502 GGTACC G^GTACC
AcyI 151 GGCGCC G[GA]^CG[CT]C
AflII 522 CTTAAG C^TTAAG
AocI 1453 CCTCAGG CC^T[ACGT]AGG
Aor51HI 991 AGCGCT AGC^GCT
Asp718I 1502 GGTACC G^GTACC
AspI 1974 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 975 CCTAGG C^CTAGG
BbeI 151 GGCGCC GGCGC^C
BbvCI 1283 CCTCAGC CCTCAGC(-5/-2)
BciVI 607 GTATCC GTATCC(6/5)
BfrI 522 CTTAAG C^TTAAG
BlnI 975 CCTAGG C^CTAGG
BsaHI 151 GGCGCC G[GA]^CG[CT]C
BsmI 868 GAATGC GAATGC(1/-1)
BspLU11I 1720 ACATGT A^CATGT
BsrDI 2520 CATTGC GCAATG(2/0)
BstAPI 441 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2486 TTCGAA TT^CGAA
BstEII 1277 GGTCACC G^GT[ACGT]ACC
BstPI 1277 GGTCACC G^GT[ACGT]ACC
BstYI 2711 AGATCC [GA]^GATC[CT]
Bsu36I 1453 CCTCAGG CC^T[ACGT]AGG
CpoI 1119 CGGACCG CG^G[AT]CCG
DraIII 2650 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 37 CGGCCG C^GGCCG
EclXI 37 CGGCCG C^GGCCG
Eco47III 991 AGCGCT AGC^GCT
Eco52I 37 CGGCCG C^GGCCG
Eco81I 1453 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1277 GGTCACC G^GT[ACGT]ACC
EcoRI 2404 GAATTC G^AATTC
Hin1I 151 GGCGCC G[GA]^CG[CT]C
HincII 2266 GTCAAC GT[CT]^[GA]AC
HindII 2266 GTCAAC GT[CT]^[GA]AC
HindIII 1691 AAGCTT A^AGCTT
KasI 151 GGCGCC G^GCGCC
KpnI 1502 GGTACC GGTAC^C
MflI 2711 AGATCC [GA]^GATC[CT]
MslI 2691 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 151 GGCGCC GG^CGCC
NruI 1086 TCGCGA TCG^CGA
NspV 2486 TTCGAA TT^CGAA
PflFI 1974 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2511 CGATCG CGAT^CG
RsrII 1119 CGGACCG CG^G[AT]CCG
ScaI 2210 AGTACT AGT^ACT
SexAI 323 ACCTGGT A^CC[AT]GGT
SfoI 151 GGCGCC GGC^GCC
SfuI 2486 TTCGAA TT^CGAA
SmaI 108 CCCGGG CCC^GGG
TfiI 72 GAATC G^A[AT]TC
Tth111I 1974 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 314 CCACAGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2711 AGATCC [GA]^GATC[CT]
XmaCI 108 CCCGGG C^CCGGG
XmaI 108 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 28 GTCTAC GT^[AC][GT]AC
1029 GTCTAC
Asp700I 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
784 GAACAGCTTC
BbrPI 2137 CACGTG CAC^GTG
2182 CACGTG
BsaAI 2137 CACGTG [CT]AC^GT[GA]
2182 CACGTG
BseRI 59 GAGGAG GAGGAG(10/8)
1954 GAGGAG
BsiEI 37 CGGCCG CG[GA][CT]^CG
2511 CGATCG
Bsp1407I 2133 TGTACA T^GTACA
2336 TGTACA
BspMI 1669 ACCTGC ACCTGC(4/8)
2633 ACCTGC
BsrGI 2133 TGTACA T^GTACA
2336 TGTACA
EarI 1315 CTCTTC CTCTTC(1/4)
2431 CTCTTC
EcoNI 330 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
555 CCTGAGCGAGG
Ksp632I 1315 CTCTTC CTCTTC(1/4)
2431 CTCTTC
MseI 523 TTAA T^TAA
545 TTAA
NcoI 740 CCATGG C^CATGG
2240 CCATGG
PmaCI 2137 CACGTG CAC^GTG
2182 CACGTG
PmlI 2137 CACGTG CAC^GTG
2182 CACGTG
PpuMI 1229 GGGACCT [GA]G^G[AT]CC[CT]
1263 GGGACCC
PstI 169 CTGCAG CTGCA^G
995 CTGCAG
SacI 1005 GAGCTC GAGCT^C
1712 GAGCTC
SphI 2318 GCATGC GCATG^C
2524 GCATGC
SspBI 2133 TGTACA T^GTACA
2336 TGTACA
StuI 641 AGGCCT AGG^CCT
2156 AGGCCT
Tru9I 523 TTAA T^TAA
545 TTAA
XmnI 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
784 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseAI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MroI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I