Restriction map for
pFN21AE1198
(Linear) (Six-base) MAPSORT of: KIEE1198.seq Check: 1932 from: 1 to: 2754
KIEE1198.orf
With 182 enzymes: SgfI *
July 15, 2010 18:34 ..
AccI GT'mk_AC
Cuts at: 0 29 1030 2754
Size: 29 1001 1724
Fragments arranged by size:
1724 1001 29
Acc65I G'GTAC_C
Cuts at: 0 1502 2754
Size: 1502 1252
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 932 1778 1909 2418 2754
Size: 568 364 846 131 509 336
Fragments arranged by size:
846 568 509 364 336 131
AfeI AGC'GCT
Cuts at: 0 993 2754
Size: 993 1761
AflII C'TTAA_G
Cuts at: 0 522 2754
Size: 522 2232
AflIII A'CryG_T
Cuts at: 0 1720 2136 2181 2754
Size: 1720 416 45 573
Fragments arranged by size:
1720 573 416 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 451 2618 2754
Size: 451 2167 136
Fragments arranged by size:
2167 451 136
AlwNI CAG_nnn'CTG
Cuts at: 0 75 406 447 1196 1461 1971 2754
Size: 75 331 41 749 265 510 783
Fragments arranged by size:
783 749 510 331 265 75 41
ApaI G_GGCC'C
Cuts at: 0 115 508 1570 2754
Size: 115 393 1062 1184
Fragments arranged by size:
1184 1062 393 115
ApoI r'AATT_y
Cuts at: 0 1810 2404 2470 2754
Size: 1810 594 66 284
Fragments arranged by size:
1810 594 284 66
AvaI C'yCGr_G
Cuts at: 0 108 382 645 2754
Size: 108 274 263 2109
Fragments arranged by size:
2109 274 263 108
AvrII C'CTAG_G
Cuts at: 0 975 2754
Size: 975 1779
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1756 1789 2754
Size: 1756 33 965
Fragments arranged by size:
1756 965 33
BanI G'GyrC_C
Cuts at: 0 119 151 1502 2360 2754
Size: 119 32 1351 858 394
Fragments arranged by size:
1351 858 394 119 32
BanII G_rGCy'C
Cuts at: 0 115 508 654 1009 1570 1716 1881 2228
Size: 115 393 146 355 561 146 165 347
Cuts at: 2228 2754
Size: 526
Fragments arranged by size:
561 526 393 355 347 165 146 146
115
BbeI G_GCGC'C
Cuts at: 0 155 2754
Size: 155 2599
BbsI GAAGACnn'nnnn_
Cuts at: 0 1672 1731 1834 2754
Size: 1672 59 103 920
Fragments arranged by size:
1672 920 103 59
BbvCI CC'TCA_GC
Cuts at: 0 1284 2754
Size: 1284 1470
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1088 2068 2102 2595 2629 2754
Size: 1054 34 980 34 493 34 125
Fragments arranged by size:
1054 980 493 125 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 618 2754
Size: 618 2136
BglI GCCn_nnn'nGGC
Cuts at: 0 317 418 649 862 1201 1247 2754
Size: 317 101 231 213 339 46 1507
Fragments arranged by size:
1507 339 317 231 213 101 46
Bme1580I G_kGCm'C
Cuts at: 0 115 508 688 858 1532 1570 2708 2754
Size: 115 393 180 170 674 38 1138 46
Fragments arranged by size:
1138 674 393 180 170 115 46 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1532 2125 2754
Size: 1532 593 629
Fragments arranged by size:
1532 629 593
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 489 2709 2754
Size: 489 2220 45
Fragments arranged by size:
2220 489 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 428 1189 2256 2754
Size: 391 37 761 1067 498
Fragments arranged by size:
1067 761 498 391 37
Bpu10I CC'TnA_GC
Cuts at: 0 556 1284 2754
Size: 556 728 1470
Fragments arranged by size:
1470 728 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2354 2538 2754
Size: 2354 184 216
Fragments arranged by size:
2354 216 184
BsaAI yAC'GTr
Cuts at: 0 2139 2184 2754
Size: 2139 45 570
Fragments arranged by size:
2139 570 45
BsaHI Gr'CG_yC
Cuts at: 0 152 2754
Size: 152 2602
BsaWI w'CCGG_w
Cuts at: 0 459 1051 1588 2670 2754
Size: 459 592 537 1082 84
Fragments arranged by size:
1082 592 537 459 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 2754
Size: 401 30 2323
Fragments arranged by size:
2323 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 74 1969 2754
Size: 74 1895 785
Fragments arranged by size:
1895 785 74
BseYI C'CCAG_C
Cuts at: 0 18 501 2754
Size: 18 483 2253
Fragments arranged by size:
2253 483 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1277 2614 2754
Size: 28 1249 1337 140
Fragments arranged by size:
1337 1249 140 28
BsiEI CG_ry'CG
Cuts at: 0 40 2514 2754
Size: 40 2474 240
Fragments arranged by size:
2474 240 40
BsiHKAI G_wGCw'C
Cuts at: 0 910 1009 1059 1716 2754
Size: 910 99 50 657 1038
Fragments arranged by size:
1038 910 657 99 50
BsmI GAATG_Cn'
Cuts at: 0 874 2754
Size: 874 1880
Bsp1286I G_dGCh'C
Cuts at: 0 115 508 654 688 858 910 1009 1059
Size: 115 393 146 34 170 52 99 50
Cuts at: 1059 1532 1570 1716 1881 2228 2708 2754
Size: 473 38 146 165 347 480 46
Fragments arranged by size:
480 473 393 347 170 165 146 146
115 99 52 50 46 38 34
BspEI T'CCGG_A
Cuts at: 0 459 1051 1588 2670 2754
Size: 459 592 537 1082 84
Fragments arranged by size:
1082 592 537 459 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1678 2642 2754
Size: 1678 964 112
Fragments arranged by size:
1678 964 112
BsrDI GCAATG_nn'
Cuts at: 0 2519 2754
Size: 2519 235
BsrGI T'GTAC_A
Cuts at: 0 2133 2336 2754
Size: 2133 203 418
Fragments arranged by size:
2133 418 203
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 2754
Size: 447 2307
BstBI TT'CG_AA
Cuts at: 0 2487 2754
Size: 2487 267
BstEII G'GTnAC_C
Cuts at: 0 1277 2754
Size: 1277 1477
BstXI CCAn_nnnn'nTGG
Cuts at: 0 747 1342 2583 2754
Size: 747 595 1241 171
Fragments arranged by size:
1241 747 595 171
BstYI r'GATC_y
Cuts at: 0 2711 2754
Size: 2711 43
Bsu36I CC'TnA_GG
Cuts at: 0 1454 2754
Size: 1454 1300
BtgI C'CryG_G
Cuts at: 0 416 740 2240 2754
Size: 416 324 1500 514
Fragments arranged by size:
1500 514 416 324
BtsI GCAGTG_nn'
Cuts at: 0 734 1222 2422 2754
Size: 734 488 1200 332
Fragments arranged by size:
1200 734 488 332
DraIII CAC_nnn'GTG
Cuts at: 0 2655 2754
Size: 2655 99
EaeI y'GGCC_r
Cuts at: 0 37 419 863 1115 2754
Size: 37 382 444 252 1639
Fragments arranged by size:
1639 444 382 252 37
EagI C'GGCC_G
Cuts at: 0 37 2754
Size: 37 2717
EarI CTCTTCn'nnn_
Cuts at: 0 1321 2437 2754
Size: 1321 1116 317
Fragments arranged by size:
1321 1116 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1124 2754
Size: 1124 1630
EcoICRI GAG'CTC
Cuts at: 0 1007 1714 2754
Size: 1007 707 1040
Fragments arranged by size:
1040 1007 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 428 568 932 1189 1778 1909 2256
Size: 391 37 140 364 257 589 131 347
Cuts at: 2256 2418 2754
Size: 162 336
Fragments arranged by size:
589 391 364 347 336 257 162 140
131 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 334 559 2754
Size: 334 225 2195
Fragments arranged by size:
2195 334 225
EcoO109I rG'GnC_Cy
Cuts at: 0 112 310 505 1230 1264 1567 2754
Size: 112 198 195 725 34 303 1187
Fragments arranged by size:
1187 725 303 198 195 112 34
EcoRI G'AATT_C
Cuts at: 0 2404 2754
Size: 2404 350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1758 2754
Size: 1758 996
HaeII r_GCGC'y
Cuts at: 0 155 995 1196 2754
Size: 155 840 201 1558
Fragments arranged by size:
1558 840 201 155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 380 412 1570 1602 2754
Size: 380 32 1158 32 1152
Fragments arranged by size:
1158 1152 380 32 32
HincII GTy'rAC
Cuts at: 0 2268 2754
Size: 2268 486
HindIII A'AGCT_T
Cuts at: 0 1691 2754
Size: 1691 1063
KasI G'GCGC_C
Cuts at: 0 151 2754
Size: 151 2603
KpnI G_GTAC'C
Cuts at: 0 1506 2754
Size: 1506 1248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2754
Size: 743 2011
MslI CAynn'nnrTG
Cuts at: 0 2695 2754
Size: 2695 59
MspA1I CmG'CkG
Cuts at: 0 447 478 530 538 626 835 1072 1179
Size: 447 31 52 8 88 209 237 107
Cuts at: 1179 1261 1756 2754
Size: 82 495 998
Fragments arranged by size:
998 495 447 237 209 107 88 82
52 31 8
NarI GG'CG_CC
Cuts at: 0 152 2754
Size: 152 2602
NcoI C'CATG_G
Cuts at: 0 740 2240 2754
Size: 740 1500 514
Fragments arranged by size:
1500 740 514
NruI TCG'CGA
Cuts at: 0 1088 2754
Size: 1088 1666
NspI r_CATG'y
Cuts at: 0 807 1608 1724 2322 2528 2754
Size: 807 801 116 598 206 226
Fragments arranged by size:
807 801 598 226 206 116
PciI A'CATG_T
Cuts at: 0 1720 2754
Size: 1720 1034
PflMI CCAn_nnn'nTGG
Cuts at: 0 406 2246 2452 2754
Size: 406 1840 206 302
Fragments arranged by size:
1840 406 302 206
PfoI T'CCnGG_A
Cuts at: 0 329 1009 2024 2215 2272 2329 2582 2754
Size: 329 680 1015 191 57 57 253 172
Fragments arranged by size:
1015 680 329 253 191 172 57 57
PmlI CAC'GTG
Cuts at: 0 2139 2184 2754
Size: 2139 45 570
Fragments arranged by size:
2139 570 45
PpuMI rG'GwC_Cy
Cuts at: 0 1230 1264 2754
Size: 1230 34 1490
Fragments arranged by size:
1490 1230 34
PspOMI G'GGCC_C
Cuts at: 0 111 504 1566 2754
Size: 111 393 1062 1188
Fragments arranged by size:
1188 1062 393 111
PstI C_TGCA'G
Cuts at: 0 173 999 2754
Size: 173 826 1755
Fragments arranged by size:
1755 826 173
PvuI CG_AT'CG
Cuts at: 0 2514 2754
Size: 2514 240
PvuII CAG'CTG
Cuts at: 0 447 478 530 1072 2754
Size: 447 31 52 542 1682
Fragments arranged by size:
1682 542 447 52 31
RsrII CG'GwC_CG
Cuts at: 0 1120 2754
Size: 1120 1634
SacI G_AGCT'C
Cuts at: 0 1009 1716 2754
Size: 1009 707 1038
Fragments arranged by size:
1038 1009 707
SanDI GG'GwC_CC
Cuts at: 0 1264 2754
Size: 1264 1490
ScaI AGT'ACT
Cuts at: 0 2212 2754
Size: 2212 542
SexAI A'CCwGG_T
Cuts at: 0 323 2754
Size: 323 2431
SfcI C'TryA_G
Cuts at: 0 169 995 1424 1912 2754
Size: 169 826 429 488 842
Fragments arranged by size:
842 826 488 429 169
SfoI GGC'GCC
Cuts at: 0 153 2754
Size: 153 2601
SmaI CCC'GGG
Cuts at: 0 110 2754
Size: 110 2644
SmlI C'TyrA_G
Cuts at: 0 522 2369 2553 2754
Size: 522 1847 184 201
Fragments arranged by size:
1847 522 201 184
SphI G_CATG'C
Cuts at: 0 2322 2528 2754
Size: 2322 206 226
Fragments arranged by size:
2322 226 206
StuI AGG'CCT
Cuts at: 0 643 2158 2754
Size: 643 1515 596
Fragments arranged by size:
1515 643 596
StyI C'CwwG_G
Cuts at: 0 665 710 740 975 2153 2240 2754
Size: 665 45 30 235 1178 87 514
Fragments arranged by size:
1178 665 514 235 87 45 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 137 739 1688 2754
Size: 137 602 949 1066
Fragments arranged by size:
1066 949 602 137
TatI w'GTAC_w
Cuts at: 0 2133 2210 2336 2754
Size: 2133 77 126 418
Fragments arranged by size:
2133 418 126 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 2395 2427 2754
Size: 401 32 1962 32 327
Fragments arranged by size:
1962 401 327 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1977 2754
Size: 1977 777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 2754
Size: 321 2433
XmaI C'CCGG_G
Cuts at: 0 108 2754
Size: 108 2646
XmnI GAAnn'nnTTC
Cuts at: 0 76 788 2754
Size: 76 712 1966
Fragments arranged by size:
1966 712 76
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AflIII AlfI AlwNI
ApaI ApoI AvaI AvrII BaeI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI
NruI NspI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SanDI ScaI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI BamHI BclI BfrBI BglII BlpI
BmgBI BmtI BsaI BsaBI BsiWI BsmBI BspHI BsrBI
BsrFI BssHII BssSI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoRV FseI FspI FspAI HpaI MfeI MluI
MscI NaeI NdeI NgoMIV NheI NotI NsiI PacI
PasI PmeI PpiI PshAI PsiI PspXI PsrI SacII
SalI SapI SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI ZraI