################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII BalI BamHI BclI BglII BlpI Bpu1102I BsaBI BsaI BsiWI BsmBI BspDI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstZ17I CelII Cfr10I ClaI DraI DrdI Eam1105I EcoRV EcoT22I FbaI FseI FspI HpaI KspI MamI MfeI MluI MluNI MscI MunI NaeI NdeI NgoMIV NheI NotI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RcaI SacII SalI SapI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1499 GGTACC G^GTACC AcyI 151 GGCGCC G[GA]^CG[CT]C AflII 519 CTTAAG C^TTAAG AocI 1450 CCTCAGG CC^T[ACGT]AGG Aor51HI 988 AGCGCT AGC^GCT Asp718I 1499 GGTACC G^GTACC AspI 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 972 CCTAGG C^CTAGG BbeI 151 GGCGCC GGCGC^C BbvCI 1280 CCTCAGC CCTCAGC(-5/-2) BciVI 604 GTATCC GTATCC(6/5) BfrI 519 CTTAAG C^TTAAG BlnI 972 CCTAGG C^CTAGG BsaHI 151 GGCGCC G[GA]^CG[CT]C BsmI 865 GAATGC GAATGC(1/-1) BspLU11I 1717 ACATGT A^CATGT BsrDI 2517 CATTGC GCAATG(2/0) BstAPI 438 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2483 TTCGAA TT^CGAA BstEII 1274 GGTCACC G^GT[ACGT]ACC BstPI 1274 GGTCACC G^GT[ACGT]ACC BstYI 2708 AGATCC [GA]^GATC[CT] Bsu36I 1450 CCTCAGG CC^T[ACGT]AGG CpoI 1116 CGGACCG CG^G[AT]CCG DraIII 2647 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 37 CGGCCG C^GGCCG EclXI 37 CGGCCG C^GGCCG Eco47III 988 AGCGCT AGC^GCT Eco52I 37 CGGCCG C^GGCCG Eco81I 1450 CCTCAGG CC^T[ACGT]AGG EcoO65I 1274 GGTCACC G^GT[ACGT]ACC EcoRI 2401 GAATTC G^AATTC Hin1I 151 GGCGCC G[GA]^CG[CT]C HincII 2263 GTCAAC GT[CT]^[GA]AC HindII 2263 GTCAAC GT[CT]^[GA]AC HindIII 1688 AAGCTT A^AGCTT KasI 151 GGCGCC G^GCGCC KpnI 1499 GGTACC GGTAC^C MflI 2708 AGATCC [GA]^GATC[CT] MslI 2688 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 151 GGCGCC GG^CGCC NruI 1083 TCGCGA TCG^CGA NspV 2483 TTCGAA TT^CGAA PflFI 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 2508 CGATCG CGAT^CG RsrII 1116 CGGACCG CG^G[AT]CCG ScaI 2207 AGTACT AGT^ACT SexAI 320 ACCTGGT A^CC[AT]GGT SfoI 151 GGCGCC GGC^GCC SfuI 2483 TTCGAA TT^CGAA SmaI 108 CCCGGG CCC^GGG TfiI 72 GAATC G^A[AT]TC Tth111I 1971 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 311 CCACAGGCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 2708 AGATCC [GA]^GATC[CT] XmaCI 108 CCCGGG C^CCGGG XmaI 108 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 28 GTCTAC GT^[AC][GT]AC 1026 GTCTAC Asp700I 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 781 GAACAGCTTC BbrPI 2134 CACGTG CAC^GTG 2179 CACGTG BsaAI 2134 CACGTG [CT]AC^GT[GA] 2179 CACGTG BseRI 59 GAGGAG GAGGAG(10/8) 1951 GAGGAG BsiEI 37 CGGCCG CG[GA][CT]^CG 2508 CGATCG Bsp1407I 2130 TGTACA T^GTACA 2333 TGTACA BspMI 1666 ACCTGC ACCTGC(4/8) 2630 ACCTGC BsrGI 2130 TGTACA T^GTACA 2333 TGTACA EarI 1312 CTCTTC CTCTTC(1/4) 2428 CTCTTC EcoNI 327 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 552 CCTGAGCGAGG Ksp632I 1312 CTCTTC CTCTTC(1/4) 2428 CTCTTC MseI 520 TTAA T^TAA 542 TTAA NcoI 737 CCATGG C^CATGG 2237 CCATGG PmaCI 2134 CACGTG CAC^GTG 2179 CACGTG PmlI 2134 CACGTG CAC^GTG 2179 CACGTG PpuMI 1226 GGGACCT [GA]G^G[AT]CC[CT] 1260 GGGACCC PstI 169 CTGCAG CTGCA^G 992 CTGCAG SacI 1002 GAGCTC GAGCT^C 1709 GAGCTC SphI 2315 GCATGC GCATG^C 2521 GCATGC SspBI 2130 TGTACA T^GTACA 2333 TGTACA StuI 638 AGGCCT AGG^CCT 2153 AGGCCT Tru9I 520 TTAA T^TAA 542 TTAA XmnI 72 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 781 GAACAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BsaWI BseAI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspEI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MroI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I